LOCUS       sequence-330723-       14217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins. Two BsaI sites for spacer 
            cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 14217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS21: pHelper(PmcCAST)_entry
FEATURES             Location/Qualifiers
     source          1..14217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(348..1430)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1392..1411
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1431..1508)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1554..1572
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1573..1597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1840..1858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1859..1883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5782..5829
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5975..5993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5994..6018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        11342..11360
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11361..11385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12453..12500
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12621..13412)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13413..13504)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaggtggtt ttttcgaagg ttaagtcagt tggggaactg cttaaccttc gatcaaacca
       61 cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct
      121 caagaagatc ctttgatctt ttctactgaa ccgctctaga tttcagtgca atttatctct
      181 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
      241 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
      301 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
      361 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      421 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
      481 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
      541 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
      601 tcggtatcgt cgtatcccac taccgagatg tccgcaccaa cgcgcagccc ggactcggta
      661 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
      721 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
      781 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
      841 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
      901 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
      961 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     1021 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     1081 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     1141 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     1201 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     1261 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     1321 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     1381 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     1441 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     1501 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga aattaatacg
     1561 actcactata ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa
     1621 ctttaataag gagatatacc atggtgagtc gtggctttat gccgttaggc gttgctcaaa
     1681 gagacctctg gtctcagtga gtcgtggctt tatgccgtta ggcgttgctc aagatccgaa
     1741 ttcgagctcg gcgcgcctgc aggtcgacaa gcttgcggcc gcataatgct taagtcgaac
     1801 agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc actatagggg
     1861 aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa gaaggagata
     1921 tacatatgac agctatatta attcaaagtg aggaatatgt agacttaaca tttaagctaa
     1981 gaggcgcacc gattcctctt gacaacggtt atcttaccta cgctgcctta tcccgtattt
     2041 gtcccccact acatgaacta aagtctattg ggattcatcc gattgctgga ataccgacca
     2101 ggaacaattt gcttgagcta actgctcaat ctcgtctaaa aattcggatt tatcatcagc
     2161 aaattccttt aatttatcct tatttagcgg gtcaagcttt tcatataggt caaaattttt
     2221 atcaactaga tatccccgac tacaaaccgt taatttcttc agaaagtgtc tattccagat
     2281 tagtgataat taaagggttc caagactcta ctaactttat tgaagctgtg caacggcaaa
     2341 tggataattt aggaatacaa ggaaaaattg aacttcttac ccgacaagat ggaacacctc
     2401 aacgaagaca attaaccatc aataaagaag ggaaacagtt taaagttaga ggatttggcg
     2461 taaaaatcag tgaactcaat cctgaagatt ccttaacctt gcaagaacag ggaattggcg
     2521 ggaaacgaaa gatgatgtgt ggaatatttg tcccagcgac tcgcagcaag gaagaagagg
     2581 aaacctgatg atagtctcta cccaacccaa aatttcccta tctctccatg ctgcggatac
     2641 gacaataatg caccgcgtcg gaatgaccgg actttacatg actttaaagc gattggaaaa
     2701 gcaatatccc ttatctcgtc aacggggagg acatatatca tggtttttga ctgctgatac
     2761 tattgaatta ttctgggaag gaagtgattt catcgcctta tcttggttaa ttaatgagtc
     2821 ttttcaatta gatgatacag gtttaattca tttagtagga ttagacaatg atcgaataga
     2881 tttaaggcag aaaatccata ttcatgaggg aatatgcggt gtttttctac gtcttaataa
     2941 gttttatcaa gcaggagaga taattaacac tgaactaagg tttgaagaaa aacaagtcga
     3001 gtatcaatat aaatccctaa cttggtacgc acatcaaacc tttgcagaga agttatgcga
     3061 ggcagatacc caacaactca gacatgatta cattcaaata accagttggt tgtatttagg
     3121 gggaattgtt cgccatgctc ggacgcagaa tactacaaaa ctagaagaga aacctgaata
     3181 cgctttagca ttattatttg taccagtagt ttgtcattat tgcttacttc atataccatc
     3241 agaagactta aaagaaagga aaccccatcg ctacttggtg gtgattccag aaataaagga
     3301 ttttgaagac gcttctcaaa ggagatggcg attacagcaa ttagaaacta aacagtttca
     3361 tgttagtagc cttggggaag caggattact ttattacagc ttagacgata ttcagcctga
     3421 agtggcctac tatcaagctt gtcaagtttg gttatatgaa aaaacaaata aagcttctcg
     3481 tcaacggaca ttgatgagta tagaagaaat taaaattgat aagaatattt taatcactta
     3541 tcaacaggtg caaaaatact ttaaaactaa ttatcagata attaaatata agcaaatttt
     3601 tattaaggtc aatccgattc gctccctgat tgctgataat ttagtgaaag gaatccattg
     3661 gtggtctaat ttttgggaaa aattggtgat agaggattca aaagaatatt tatttaacca
     3721 gttattttcc aatcgagaag gattcatcat aatggcagag aatagtgaag aagacaagca
     3781 gtatcttatt tttattaagg tatttcagca agctatgaag ggcaactttg ctaaaattta
     3841 tgccaaaaca gaggaaggta aagatcctcc aattaagaag aaagttgaac gattaagggc
     3901 agagctaaac tattgttatg atgaattgtc gtttaaggaa tatttgtctg actttttagt
     3961 aagaggggga ttaaataagt attttaatga gcatcaggaa gagattgcac tactaattaa
     4021 aaaatcacct tggcaagaaa taagaatttg gtcattgtta gcgatcgcca gttataaacc
     4081 caaagataaa ctcactaata gggatgatag ttcattgtca aacaatcaaa aactagaaga
     4141 agtgaatgat gactcagaag aagaatgaca gtaatatccc aaattattat ctttacggaa
     4201 cagtcctcac tcgttatggt ttagcttcct taaatcatga cattagacga ggaaataaga
     4261 ctattctgca aaaaggctat tggaataatg gtaaaattca ttcttttgta ggttcaagtg
     4321 caattcgttg ggcgttacgt ttttatcttc aaaagcaagg ttatttagtt aatcgagtct
     4381 gggatgagga agaacatatt aatcggttaa caagtgaaga tttcgatcca gaaaaatttt
     4441 atgatgatga tatttttggg tttgctttat tagagtctgc tgaaacagag gaggacactt
     4501 caacaacaaa gagaaaaaag aaacaaacaa agaccagcac tcctaatcag cgaatgggtg
     4561 ctttagggat gaatatggct gtttcgctaa cgccttatga tggtgcagtt aagttagggg
     4621 caaaaagtgg cagagagaag gatagtacat ctctccattt tacagaatat catgcaactc
     4681 gatatcaata ttattttgga attgatgcca ctcatctcaa agatttttcg cggattttac
     4741 ctatgataga tgggattatg aatctgccca aggtaggagg gagtagtaat atttttaatt
     4801 atcctttctg tcccgatagt ttggtatttc aatggacaaa tcattttgct tcatatattt
     4861 cctattgctt tgaatattgc gatcctaaga gtaaggaagc aaaactttca caagagttta
     4921 tagatgaagt ggaatgcgga cagattgacc ctagtaaatt gtggattggg ggaacaattg
     4981 ttaaggattt acagcaattg gataattttg aaagttcccc tttgaacaag gcgcatattt
     5041 atcgtaatcg aaatgaaatg attgaagctt tgaaaacagt cattaaaaga gatttgggct
     5101 tggaagaatc aaaatgattg caccattaat tctctatttg gatgttccat ttacgacttt
     5161 tcgggagtcc catgcaaggg aaatgggaaa aacctatcct gttcctcctc cagcgacagt
     5221 gtatgggatg ttgttgtctt tggtaggaga aacgaatgtc tatcgccact gtggggtaga
     5281 attggccatt gcaatgttat ctagccccaa aaagtcgcga attttgcgtc aaatgagacg
     5341 gtttaagaat gctgatttta gccatccaga gaatgttatt ccctgttatc aagaaatttt
     5401 gtctaatctg aagtgtttga tttgggttcg ttctgacgag gaaaagatac aaccaagttt
     5461 aagggagagg atacagttag cgtttgacca tcctgagcta gtaagacggt ttggttgttt
     5521 atttttggga gaaagtgacc agttgattaa aacgatcaaa cttgcaaggg aagattatct
     5581 agagggagtg agacagtggg cgattagaga taatcgaggc aggttaactt taccctattg
     5641 ggttgaccat gtggggtcaa gaaatacccg atttttgagg tatcggattg aggaaatgga
     5701 tagattatca cctcctgatt tagcctggac aatggttcaa tctccgattt gacctaggct
     5761 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     5821 ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc
     5881 aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctc gggatctcga
     5941 cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat aggggaattg
     6001 tgagcggata acaattcccc tgtagaaata attttgttta actttaataa ggagatatac
     6061 catgaaagag caatttgaac aatgggttgc tcgccttcaa atatccgagg cagggcaaaa
     6121 aattattgag aaagttcgct catctgagcc atcccgtcgc gttggtggcg gtagcaagaa
     6181 tgtttcggga cgctacccta gtcgcaagat gggagtcacg attcaatttg agtctcatcg
     6241 ggttgaactg ccaattatct atcagttaga acatgataaa gatgttctag aattctacga
     6301 tcaaccaccg caaattaaac ttgactatca agcaagtaac ggacgtagat taggcgtttt
     6361 acacacccca gatttatttg tcatccgaac caattcggct ggttgggaag agtgtaaaac
     6421 tgagcaggat ttgaaaaaac tagcagagaa aaaccccaat cgttactttt actctgaaga
     6481 aaataatcaa tggcattcct tgccaggaga agtttatgct aaccagtttg ggctttatca
     6541 ccgcattcat tcagatcgcg aaatcaattg ggttctgcaa cgtaatttgc aatttttaga
     6601 agattattat cgctcagaat ctctagtagt ggaagaagcg atcgctcaat cattacttgc
     6661 aattgtgtca tctcaaccag gaatcacttt agcagaatta ctcaatcggt caacaggagc
     6721 taaatctgac gatatctaca ccttaattat tcaagagcaa atctatattg atttaaatgc
     6781 tacaccacta gctgaaccag aaagatgtct aatcttccgt gacgagcaaa ctgctagtgc
     6841 ttatagatta atggttgagc aaccaagtgt aagtcttccg gctatctctc ctgtagtcaa
     6901 tattgttact ggagcaactg ttagttggga cggcaaaggt ttaaatatca tccatgttgg
     6961 agaaacagag gttatacttg gcgcagaaaa taatcagtta attgaactca aaaaagctat
     7021 atttgagaat ctggttcgcc aaggaaaaat cacaagtctc caaacaccag aaaaaacagc
     7081 tattagcaca gaatcttggc agcgatttca tcaagctagt cctgaagacc aagcagaagc
     7141 ccttgaacgc tacaaaacta tcgaacctta cttaaacgga catcctccag aaaacgaaac
     7201 aataccagcc cgtaccattc gtcactggaa agctaaatat ttaattgcaa ttcaaaaata
     7261 cggttgcggc tacattgggc ttttgtccca tcgcagtgtc aaaggtaatc gacagcgcaa
     7321 gctgcccgaa gacaccttag caattatgga gaagtttatt ttagaagact atgaaactct
     7381 taaacaaaaa agaatgtggg aggttcacgc ggctttagtt cgagcttgcg aagaagctgg
     7441 tgtaattgct ccgagctata aagcatttac caaagaagta caacgccgga ctggttacga
     7501 acagactaaa aaacgccaag gtcgtcgtgc cgcttatcag catgaatcct tctattggga
     7561 gcttgctata actaccccaa gacacggcga tcgccctttt gaaattgggc atatagatca
     7621 tactgagtta gatgtagagc tagtttgctc tgatacaggt cgcaatcttg gcagaccttg
     7681 ggcaacattt ctcgtagatg cttattcacg gcgattgctg gcagtttacc taacgtttga
     7741 ctcaccctca tatcgttctt gcttaatggt gctaagaatt tgtgtcaagc gtcatggcag
     7801 gcttccccaa attgtagtgg tagataacgg tgcagagttt cacagcgttt actttgaaac
     7861 actactagct acttttgaat gtacaaaaaa gcaaagaccc ccagccaaag ctcgttttgg
     7921 ttcggtttgc gaacgcctat ttggaacttc caatactcaa tttgtccata acttacttgg
     7981 taatacacaa atcactcgta atgtccgcca agtcactaag tcagttaacc ctaaaaatct
     8041 agctgtatgg acacttggtt tactttatga atatctttgt gcttgggctt acgaagttta
     8101 tgatacagac gaacatccgg cacttttcca gagtccgagg gatgcgtttg ctgcgggtat
     8161 ggcgattgga ggcagtcggg ttcaccgcat gattccttac gatgaaaact ttcagattct
     8221 taccctacct acaacctcag agggaaaagc aaaagttcaa gttggacgcg gcataaaaat
     8281 taactacata tactactggt ctaatagttt ccgcgatcct caaattgaaa atacctctgt
     8341 gcagataaga tacgacccgt ttaatattgg catcgcttac gcctttgttc gaggacagtg
     8401 ggtacagtgt atctctcaat attatgcaga actccaaggt cgttcagaaa aagaactgaa
     8461 gttagctagt atcgagttac gcaaacacta ttcaaatcat acacaacaat ctaaagtttc
     8521 ggctaagaat ttagcagaat ttttagcatc ggtagaagct caagaagctc tattagaaca
     8581 gcgttcctat gatgcagaag ttaaggaagt ctttagggta atcgagggtg gaaaagcgac
     8641 aacaagccgt aacgaagaac caaaacttat tcaagttact tttgccaata ccgattttca
     8701 agcggatgaa gatgaagcga tcgtcccaga aacacttgta gtgtatgagg agttttaact
     8761 atgacaaaat ctactggatt tccgcttgaa ctcctcacac ggcctgctac agaaaggctt
     8821 gcttactttg aaaattacac cgtagcgcat cctcgactta aagaagtcta tgaaattctc
     8881 atgcgaacga ttgccgaacc agcaggagct tcatttatct ttgtttacgg tgctagtggt
     8941 gttggtaaaa caactttgcg actccgcgtt gagcagaaat taacagaact agcattacct
     9001 aaattggaaa gcgatcgcgc tcgtgttcct gttgttggta ttgaagctat agcgccagaa
     9061 tcgagatact tcaactggaa agaatactac acacgcgcct taattacttt ggaagaacca
     9121 ttaatcgatc acaagtttga ttacggtgtg cgtggtatct ctcgcgataa ttttggcaaa
     9181 attaatgttg agtcgaaagt agttgcacct gctctacgca gagctttaga aaatgctctc
     9241 atccatcgtc atccagacgt attttttgta gatgaagcac aacattttgg taaggtggca
     9301 agcggataca aactacaaga tcaattagac tgtctcaaat ctttagcaaa tatgacaggc
     9361 atcttgcact gtctattagg aacatacgaa ctacttacat tccgtaattt gagcggtcaa
     9421 ctctcacgtc gtagtgttga tattcatttc cgccgttatt gtgctgatag cccagaagat
     9481 gtacaggctt ttaagagtgt gttgttaact tttcaacagc atctgccact agcagaaact
     9541 ccaaatttag tcgatcattg ggaatatttt tacgaacgta ctttaggatg tattggcaca
     9601 ctgaaagatt ggctgaagcg tgttcttagt gatgctttgg atcgagaagc aacaacaatc
     9661 actctcaaag atttacaaaa acgtgctttg tcggttgctc agtgtcagaa aatgttcaag
     9721 gaaattcaag agggtgaacg acaattatct gaaacagaag cagatgtgca aaacttacgc
     9781 tctgcattag gacttggcgc aaaaccaatt gtgctacccg aagaaacccc aaaaactact
     9841 cgtccgccag gaaaggttgg caaacgcaaa cctaaaagag atcccatcgg agtgcagcaa
     9901 gatgttagct agtgtattag aaacatacga atcttggaat ttaaaaaagc cattaattcc
     9961 acaacgtagt cgcctctatc aacctcaacc tgtaggtatt gggactcctt acatagagag
    10021 tttaacaggt tatatcacac ggatagcaga gttgcatgga gttcttccag gagtcttgat
    10081 gactagagaa attgctccgc ttgtcaataa aatatatttc caaaatggag ctaatcgagg
    10141 ttttcgtgaa atattcaacc gtagtcaagc gttaaatggc atgggagaga tggcagcaga
    10201 tttagttcaa gtgttgcaaa aattgacgct tagagatgac ttgcgttttt taaccatgct
    10261 gttttggtct aacattttga ctccacgcaa tttatttcgt accagaaaag cttggtgtcc
    10321 aatttgttat caagagcggc atcaaaatgg gctggtagtc tatgagcaat tgctttggac
    10381 aattaaccta attacaattt gcccgcagca tcaaaaaccc ttggttgaac tttgccctca
    10441 ctgtaatcat gaatcacctt tgttaaactg gcgctcaaga cctggttact gttcaaagtg
    10501 tggagagtgg ttaggcgcaa atcaatgcct taagacattt acagatggtg aaggatcaat
    10561 aaaattacag ttggagtggc agtattggac tgccaatgtt gtaggagagc taattttagc
    10621 ttctcaatgc tttgaatctg ccccatcaaa agaaaatatt acaaaatcat taaatatagt
    10681 tattgataaa gttgcggaaa ataatgcagc tgcattttct cgtcttattg gagtaccaaa
    10741 aaatagtttg tggatgtggc agtctactaa aaccttgcca gagctaaata cacttctgaa
    10801 aatttgttac gaattagaga tatcgttggt agaatttctt actccaaaaa atctaattac
    10861 taaatctttt acaaaaatct cgcagaagca cctccaactt tctcgcacac caagggtatc
    10921 tccaaaatct ttcgaccaat atcaagtaaa agatgcttta ttagcaatac ttgcaggtaa
    10981 tgaagaacca ccaccaacaa tggaagaagt tggaaagcgt ttaggtcatc acaacagaac
    11041 tatttctaga cactttccag atttgtgtag tgctatttct gctaaatgtc gtaattacaa
    11101 taaagcttgc cgtctcaaaa gcatagaaaa actttgcagc gaagttagag agattgtcct
    11161 tagccttaat gctcagggtg tgtatcccac agaaggtcgt gtttgtgaat taatgccaaa
    11221 tccggggtgt tttagatata aacaagtccg cgctgctttt aatgatgcac gacgtgagtt
    11281 tggtttgtaa cttaagtcga acagaaagta atcgtattgt acacggccgc ataatcgaaa
    11341 ttaatacgac tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt
    11401 agttaagtat aagaaggaga tatacatgac tatttactca gaacattgga gcctaaatcc
    11461 tctagaaatc ccgcaacgga gtcggttatt ttctcttgaa cctgtggcag tgggaactcc
    11521 ctatgcagaa agtcttagta gctatctcca tcgtttagcg caggcgcatt gtttaacctc
    11581 tgagaagctc gttatgggag aaattgcgcc acttattctc aaagatgagg ataaatctga
    11641 gctattgtca aaaaatctga gtcacctatt gggaaatagc gacgctaaac cggctattaa
    11701 cggaatgcga gaaatgacag aaaagttagt cactgtctta gaagagttaa caatgcgtca
    11761 ggatttacgc tttttgaccc ttttaagctg gaaaggaatg atttatgaca agggattatt
    11821 tcggaactat cgcgcttggt gtccttgttg ttgtgaagag tggatgcaga agaacaaaac
    11881 tatctatgaa ccattatcat ggtcatttaa ggacgtagaa ttttgtttga ttcacaaaca
    11941 gcgattaata gaagaatgtt ctcattgtgg tgcccgttta cctgtgatgg ctaggctttc
    12001 acctgcggga ttttgttctc gttgttacgg gtggttagga caggaaatta aaggcgaaga
    12061 agaaatagag aaatatcgag ttaatattca aggaattagc gaattaattg cacttactcc
    12121 ccaattaggt tataaaccca tcccgatcga gttaacccga aaattacaat tgattttgtt
    12181 agtgtttgaa caagcaattg gaaaggatgt gaagttattg ggagatttag gaggaatcat
    12241 ggaatcgttg aggatagcct caacaacaaa tcaaagtcaa ccttatcatt tggtgaagtt
    12301 gattattcct gtgtgcgaga aggcgaagat tagtgtattc cagttgtttg ggtcagattt
    12361 taaggaattg ggcaagatat tgttcgggaa ttttagtctg gaattaaagt tatagttaat
    12421 taacctaggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
    12481 gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc
    12541 ttccggtagt gccctgaacc gacgaccggg tcatcgtggc cggatcttgc ggcccctcgg
    12601 cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag
    12661 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
    12721 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
    12781 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
    12841 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
    12901 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
    12961 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
    13021 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
    13081 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
    13141 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
    13201 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
    13261 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
    13321 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
    13381 gtcgatactt cggcgatcac cgcttccctc atactcttcc tttttcaata ttattgaagc
    13441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13501 caaatagcta gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
    13561 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
    13621 acagcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
    13681 taaacagacg cttttccggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
    13741 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggcggg tcgctccctc
    13801 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgttccgcct ttctccctta
    13861 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
    13921 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
    13981 cggtaactgt tcacttgagt ccaacccgga aaagcacggt aaaacgccac tggcagcagc
    14041 cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt gctcttgaag
    14101 tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct ctgcgaaagc
    14161 cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac cacctcc
//
