LOCUS       sequence-330728-       14235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins with crRNA targeting 
            PmcPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14235)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 14235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS18: pHelper(PmcCAST)_PmcPSP1
FEATURES             Location/Qualifiers
     source          1..14235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        107..125
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    126..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      4049..4096
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4242..4260
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4261..4285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9609..9627
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9628..9652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      10720..10767
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10888..11679)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(11680..11771)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(12832..13914)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13876..13895
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13915..13992)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        14038..14056
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    14057..14081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atccgaattc gagctcggcg cgcctgcagg tcgacaagct tgcggccgca taatgcttaa
       61 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
      121 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
      181 ggagatatac atatgacagc tatattaatt caaagtgagg aatatgtaga cttaacattt
      241 aagctaagag gcgcaccgat tcctcttgac aacggttatc ttacctacgc tgccttatcc
      301 cgtatttgtc ccccactaca tgaactaaag tctattggga ttcatccgat tgctggaata
      361 ccgaccagga acaatttgct tgagctaact gctcaatctc gtctaaaaat tcggatttat
      421 catcagcaaa ttcctttaat ttatccttat ttagcgggtc aagcttttca tataggtcaa
      481 aatttttatc aactagatat ccccgactac aaaccgttaa tttcttcaga aagtgtctat
      541 tccagattag tgataattaa agggttccaa gactctacta actttattga agctgtgcaa
      601 cggcaaatgg ataatttagg aatacaagga aaaattgaac ttcttacccg acaagatgga
      661 acacctcaac gaagacaatt aaccatcaat aaagaaggga aacagtttaa agttagagga
      721 tttggcgtaa aaatcagtga actcaatcct gaagattcct taaccttgca agaacaggga
      781 attggcggga aacgaaagat gatgtgtgga atatttgtcc cagcgactcg cagcaaggaa
      841 gaagaggaaa cctgatgata gtctctaccc aacccaaaat ttccctatct ctccatgctg
      901 cggatacgac aataatgcac cgcgtcggaa tgaccggact ttacatgact ttaaagcgat
      961 tggaaaagca atatccctta tctcgtcaac ggggaggaca tatatcatgg tttttgactg
     1021 ctgatactat tgaattattc tgggaaggaa gtgatttcat cgccttatct tggttaatta
     1081 atgagtcttt tcaattagat gatacaggtt taattcattt agtaggatta gacaatgatc
     1141 gaatagattt aaggcagaaa atccatattc atgagggaat atgcggtgtt tttctacgtc
     1201 ttaataagtt ttatcaagca ggagagataa ttaacactga actaaggttt gaagaaaaac
     1261 aagtcgagta tcaatataaa tccctaactt ggtacgcaca tcaaaccttt gcagagaagt
     1321 tatgcgaggc agatacccaa caactcagac atgattacat tcaaataacc agttggttgt
     1381 atttaggggg aattgttcgc catgctcgga cgcagaatac tacaaaacta gaagagaaac
     1441 ctgaatacgc tttagcatta ttatttgtac cagtagtttg tcattattgc ttacttcata
     1501 taccatcaga agacttaaaa gaaaggaaac cccatcgcta cttggtggtg attccagaaa
     1561 taaaggattt tgaagacgct tctcaaagga gatggcgatt acagcaatta gaaactaaac
     1621 agtttcatgt tagtagcctt ggggaagcag gattacttta ttacagctta gacgatattc
     1681 agcctgaagt ggcctactat caagcttgtc aagtttggtt atatgaaaaa acaaataaag
     1741 cttctcgtca acggacattg atgagtatag aagaaattaa aattgataag aatattttaa
     1801 tcacttatca acaggtgcaa aaatacttta aaactaatta tcagataatt aaatataagc
     1861 aaatttttat taaggtcaat ccgattcgct ccctgattgc tgataattta gtgaaaggaa
     1921 tccattggtg gtctaatttt tgggaaaaat tggtgataga ggattcaaaa gaatatttat
     1981 ttaaccagtt attttccaat cgagaaggat tcatcataat ggcagagaat agtgaagaag
     2041 acaagcagta tcttattttt attaaggtat ttcagcaagc tatgaagggc aactttgcta
     2101 aaatttatgc caaaacagag gaaggtaaag atcctccaat taagaagaaa gttgaacgat
     2161 taagggcaga gctaaactat tgttatgatg aattgtcgtt taaggaatat ttgtctgact
     2221 ttttagtaag agggggatta aataagtatt ttaatgagca tcaggaagag attgcactac
     2281 taattaaaaa atcaccttgg caagaaataa gaatttggtc attgttagcg atcgccagtt
     2341 ataaacccaa agataaactc actaataggg atgatagttc attgtcaaac aatcaaaaac
     2401 tagaagaagt gaatgatgac tcagaagaag aatgacagta atatcccaaa ttattatctt
     2461 tacggaacag tcctcactcg ttatggttta gcttccttaa atcatgacat tagacgagga
     2521 aataagacta ttctgcaaaa aggctattgg aataatggta aaattcattc ttttgtaggt
     2581 tcaagtgcaa ttcgttgggc gttacgtttt tatcttcaaa agcaaggtta tttagttaat
     2641 cgagtctggg atgaggaaga acatattaat cggttaacaa gtgaagattt cgatccagaa
     2701 aaattttatg atgatgatat ttttgggttt gctttattag agtctgctga aacagaggag
     2761 gacacttcaa caacaaagag aaaaaagaaa caaacaaaga ccagcactcc taatcagcga
     2821 atgggtgctt tagggatgaa tatggctgtt tcgctaacgc cttatgatgg tgcagttaag
     2881 ttaggggcaa aaagtggcag agagaaggat agtacatctc tccattttac agaatatcat
     2941 gcaactcgat atcaatatta ttttggaatt gatgccactc atctcaaaga tttttcgcgg
     3001 attttaccta tgatagatgg gattatgaat ctgcccaagg taggagggag tagtaatatt
     3061 tttaattatc ctttctgtcc cgatagtttg gtatttcaat ggacaaatca ttttgcttca
     3121 tatatttcct attgctttga atattgcgat cctaagagta aggaagcaaa actttcacaa
     3181 gagtttatag atgaagtgga atgcggacag attgacccta gtaaattgtg gattggggga
     3241 acaattgtta aggatttaca gcaattggat aattttgaaa gttccccttt gaacaaggcg
     3301 catatttatc gtaatcgaaa tgaaatgatt gaagctttga aaacagtcat taaaagagat
     3361 ttgggcttgg aagaatcaaa atgattgcac cattaattct ctatttggat gttccattta
     3421 cgacttttcg ggagtcccat gcaagggaaa tgggaaaaac ctatcctgtt cctcctccag
     3481 cgacagtgta tgggatgttg ttgtctttgg taggagaaac gaatgtctat cgccactgtg
     3541 gggtagaatt ggccattgca atgttatcta gccccaaaaa gtcgcgaatt ttgcgtcaaa
     3601 tgagacggtt taagaatgct gattttagcc atccagagaa tgttattccc tgttatcaag
     3661 aaattttgtc taatctgaag tgtttgattt gggttcgttc tgacgaggaa aagatacaac
     3721 caagtttaag ggagaggata cagttagcgt ttgaccatcc tgagctagta agacggtttg
     3781 gttgtttatt tttgggagaa agtgaccagt tgattaaaac gatcaaactt gcaagggaag
     3841 attatctaga gggagtgaga cagtgggcga ttagagataa tcgaggcagg ttaactttac
     3901 cctattgggt tgaccatgtg gggtcaagaa atacccgatt tttgaggtat cggattgagg
     3961 aaatggatag attatcacct cctgatttag cctggacaat ggttcaatct ccgatttgac
     4021 ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     4081 cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc
     4141 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctcggg
     4201 atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactatagg
     4261 ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact ttaataagga
     4321 gatataccat gaaagagcaa tttgaacaat gggttgctcg ccttcaaata tccgaggcag
     4381 ggcaaaaaat tattgagaaa gttcgctcat ctgagccatc ccgtcgcgtt ggtggcggta
     4441 gcaagaatgt ttcgggacgc taccctagtc gcaagatggg agtcacgatt caatttgagt
     4501 ctcatcgggt tgaactgcca attatctatc agttagaaca tgataaagat gttctagaat
     4561 tctacgatca accaccgcaa attaaacttg actatcaagc aagtaacgga cgtagattag
     4621 gcgttttaca caccccagat ttatttgtca tccgaaccaa ttcggctggt tgggaagagt
     4681 gtaaaactga gcaggatttg aaaaaactag cagagaaaaa ccccaatcgt tacttttact
     4741 ctgaagaaaa taatcaatgg cattccttgc caggagaagt ttatgctaac cagtttgggc
     4801 tttatcaccg cattcattca gatcgcgaaa tcaattgggt tctgcaacgt aatttgcaat
     4861 ttttagaaga ttattatcgc tcagaatctc tagtagtgga agaagcgatc gctcaatcat
     4921 tacttgcaat tgtgtcatct caaccaggaa tcactttagc agaattactc aatcggtcaa
     4981 caggagctaa atctgacgat atctacacct taattattca agagcaaatc tatattgatt
     5041 taaatgctac accactagct gaaccagaaa gatgtctaat cttccgtgac gagcaaactg
     5101 ctagtgctta tagattaatg gttgagcaac caagtgtaag tcttccggct atctctcctg
     5161 tagtcaatat tgttactgga gcaactgtta gttgggacgg caaaggttta aatatcatcc
     5221 atgttggaga aacagaggtt atacttggcg cagaaaataa tcagttaatt gaactcaaaa
     5281 aagctatatt tgagaatctg gttcgccaag gaaaaatcac aagtctccaa acaccagaaa
     5341 aaacagctat tagcacagaa tcttggcagc gatttcatca agctagtcct gaagaccaag
     5401 cagaagccct tgaacgctac aaaactatcg aaccttactt aaacggacat cctccagaaa
     5461 acgaaacaat accagcccgt accattcgtc actggaaagc taaatattta attgcaattc
     5521 aaaaatacgg ttgcggctac attgggcttt tgtcccatcg cagtgtcaaa ggtaatcgac
     5581 agcgcaagct gcccgaagac accttagcaa ttatggagaa gtttatttta gaagactatg
     5641 aaactcttaa acaaaaaaga atgtgggagg ttcacgcggc tttagttcga gcttgcgaag
     5701 aagctggtgt aattgctccg agctataaag catttaccaa agaagtacaa cgccggactg
     5761 gttacgaaca gactaaaaaa cgccaaggtc gtcgtgccgc ttatcagcat gaatccttct
     5821 attgggagct tgctataact accccaagac acggcgatcg cccttttgaa attgggcata
     5881 tagatcatac tgagttagat gtagagctag tttgctctga tacaggtcgc aatcttggca
     5941 gaccttgggc aacatttctc gtagatgctt attcacggcg attgctggca gtttacctaa
     6001 cgtttgactc accctcatat cgttcttgct taatggtgct aagaatttgt gtcaagcgtc
     6061 atggcaggct tccccaaatt gtagtggtag ataacggtgc agagtttcac agcgtttact
     6121 ttgaaacact actagctact tttgaatgta caaaaaagca aagaccccca gccaaagctc
     6181 gttttggttc ggtttgcgaa cgcctatttg gaacttccaa tactcaattt gtccataact
     6241 tacttggtaa tacacaaatc actcgtaatg tccgccaagt cactaagtca gttaacccta
     6301 aaaatctagc tgtatggaca cttggtttac tttatgaata tctttgtgct tgggcttacg
     6361 aagtttatga tacagacgaa catccggcac ttttccagag tccgagggat gcgtttgctg
     6421 cgggtatggc gattggaggc agtcgggttc accgcatgat tccttacgat gaaaactttc
     6481 agattcttac cctacctaca acctcagagg gaaaagcaaa agttcaagtt ggacgcggca
     6541 taaaaattaa ctacatatac tactggtcta atagtttccg cgatcctcaa attgaaaata
     6601 cctctgtgca gataagatac gacccgttta atattggcat cgcttacgcc tttgttcgag
     6661 gacagtgggt acagtgtatc tctcaatatt atgcagaact ccaaggtcgt tcagaaaaag
     6721 aactgaagtt agctagtatc gagttacgca aacactattc aaatcataca caacaatcta
     6781 aagtttcggc taagaattta gcagaatttt tagcatcggt agaagctcaa gaagctctat
     6841 tagaacagcg ttcctatgat gcagaagtta aggaagtctt tagggtaatc gagggtggaa
     6901 aagcgacaac aagccgtaac gaagaaccaa aacttattca agttactttt gccaataccg
     6961 attttcaagc ggatgaagat gaagcgatcg tcccagaaac acttgtagtg tatgaggagt
     7021 tttaactatg acaaaatcta ctggatttcc gcttgaactc ctcacacggc ctgctacaga
     7081 aaggcttgct tactttgaaa attacaccgt agcgcatcct cgacttaaag aagtctatga
     7141 aattctcatg cgaacgattg ccgaaccagc aggagcttca tttatctttg tttacggtgc
     7201 tagtggtgtt ggtaaaacaa ctttgcgact ccgcgttgag cagaaattaa cagaactagc
     7261 attacctaaa ttggaaagcg atcgcgctcg tgttcctgtt gttggtattg aagctatagc
     7321 gccagaatcg agatacttca actggaaaga atactacaca cgcgccttaa ttactttgga
     7381 agaaccatta atcgatcaca agtttgatta cggtgtgcgt ggtatctctc gcgataattt
     7441 tggcaaaatt aatgttgagt cgaaagtagt tgcacctgct ctacgcagag ctttagaaaa
     7501 tgctctcatc catcgtcatc cagacgtatt ttttgtagat gaagcacaac attttggtaa
     7561 ggtggcaagc ggatacaaac tacaagatca attagactgt ctcaaatctt tagcaaatat
     7621 gacaggcatc ttgcactgtc tattaggaac atacgaacta cttacattcc gtaatttgag
     7681 cggtcaactc tcacgtcgta gtgttgatat tcatttccgc cgttattgtg ctgatagccc
     7741 agaagatgta caggctttta agagtgtgtt gttaactttt caacagcatc tgccactagc
     7801 agaaactcca aatttagtcg atcattggga atatttttac gaacgtactt taggatgtat
     7861 tggcacactg aaagattggc tgaagcgtgt tcttagtgat gctttggatc gagaagcaac
     7921 aacaatcact ctcaaagatt tacaaaaacg tgctttgtcg gttgctcagt gtcagaaaat
     7981 gttcaaggaa attcaagagg gtgaacgaca attatctgaa acagaagcag atgtgcaaaa
     8041 cttacgctct gcattaggac ttggcgcaaa accaattgtg ctacccgaag aaaccccaaa
     8101 aactactcgt ccgccaggaa aggttggcaa acgcaaacct aaaagagatc ccatcggagt
     8161 gcagcaagat gttagctagt gtattagaaa catacgaatc ttggaattta aaaaagccat
     8221 taattccaca acgtagtcgc ctctatcaac ctcaacctgt aggtattggg actccttaca
     8281 tagagagttt aacaggttat atcacacgga tagcagagtt gcatggagtt cttccaggag
     8341 tcttgatgac tagagaaatt gctccgcttg tcaataaaat atatttccaa aatggagcta
     8401 atcgaggttt tcgtgaaata ttcaaccgta gtcaagcgtt aaatggcatg ggagagatgg
     8461 cagcagattt agttcaagtg ttgcaaaaat tgacgcttag agatgacttg cgttttttaa
     8521 ccatgctgtt ttggtctaac attttgactc cacgcaattt atttcgtacc agaaaagctt
     8581 ggtgtccaat ttgttatcaa gagcggcatc aaaatgggct ggtagtctat gagcaattgc
     8641 tttggacaat taacctaatt acaatttgcc cgcagcatca aaaacccttg gttgaacttt
     8701 gccctcactg taatcatgaa tcacctttgt taaactggcg ctcaagacct ggttactgtt
     8761 caaagtgtgg agagtggtta ggcgcaaatc aatgccttaa gacatttaca gatggtgaag
     8821 gatcaataaa attacagttg gagtggcagt attggactgc caatgttgta ggagagctaa
     8881 ttttagcttc tcaatgcttt gaatctgccc catcaaaaga aaatattaca aaatcattaa
     8941 atatagttat tgataaagtt gcggaaaata atgcagctgc attttctcgt cttattggag
     9001 taccaaaaaa tagtttgtgg atgtggcagt ctactaaaac cttgccagag ctaaatacac
     9061 ttctgaaaat ttgttacgaa ttagagatat cgttggtaga atttcttact ccaaaaaatc
     9121 taattactaa atcttttaca aaaatctcgc agaagcacct ccaactttct cgcacaccaa
     9181 gggtatctcc aaaatctttc gaccaatatc aagtaaaaga tgctttatta gcaatacttg
     9241 caggtaatga agaaccacca ccaacaatgg aagaagttgg aaagcgttta ggtcatcaca
     9301 acagaactat ttctagacac tttccagatt tgtgtagtgc tatttctgct aaatgtcgta
     9361 attacaataa agcttgccgt ctcaaaagca tagaaaaact ttgcagcgaa gttagagaga
     9421 ttgtccttag ccttaatgct cagggtgtgt atcccacaga aggtcgtgtt tgtgaattaa
     9481 tgccaaatcc ggggtgtttt agatataaac aagtccgcgc tgcttttaat gatgcacgac
     9541 gtgagtttgg tttgtaactt aagtcgaaca gaaagtaatc gtattgtaca cggccgcata
     9601 atcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt ccccatctta
     9661 gtatattagt taagtataag aaggagatat acatgactat ttactcagaa cattggagcc
     9721 taaatcctct agaaatcccg caacggagtc ggttattttc tcttgaacct gtggcagtgg
     9781 gaactcccta tgcagaaagt cttagtagct atctccatcg tttagcgcag gcgcattgtt
     9841 taacctctga gaagctcgtt atgggagaaa ttgcgccact tattctcaaa gatgaggata
     9901 aatctgagct attgtcaaaa aatctgagtc acctattggg aaatagcgac gctaaaccgg
     9961 ctattaacgg aatgcgagaa atgacagaaa agttagtcac tgtcttagaa gagttaacaa
    10021 tgcgtcagga tttacgcttt ttgacccttt taagctggaa aggaatgatt tatgacaagg
    10081 gattatttcg gaactatcgc gcttggtgtc cttgttgttg tgaagagtgg atgcagaaga
    10141 acaaaactat ctatgaacca ttatcatggt catttaagga cgtagaattt tgtttgattc
    10201 acaaacagcg attaatagaa gaatgttctc attgtggtgc ccgtttacct gtgatggcta
    10261 ggctttcacc tgcgggattt tgttctcgtt gttacgggtg gttaggacag gaaattaaag
    10321 gcgaagaaga aatagagaaa tatcgagtta atattcaagg aattagcgaa ttaattgcac
    10381 ttactcccca attaggttat aaacccatcc cgatcgagtt aacccgaaaa ttacaattga
    10441 ttttgttagt gtttgaacaa gcaattggaa aggatgtgaa gttattggga gatttaggag
    10501 gaatcatgga atcgttgagg atagcctcaa caacaaatca aagtcaacct tatcatttgg
    10561 tgaagttgat tattcctgtg tgcgagaagg cgaagattag tgtattccag ttgtttgggt
    10621 cagattttaa ggaattgggc aagatattgt tcgggaattt tagtctggaa ttaaagttat
    10681 agttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
    10741 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
    10801 acactgcttc cggtagtgcc ctgaaccgac gaccgggtca tcgtggccgg atcttgcggc
    10861 ccctcggctt gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt
    10921 cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg
    10981 tccaagataa gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc
    11041 cattgcccag tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca
    11101 aatgcgggac aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt
    11161 ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa
    11221 gagttcctcc gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa
    11281 gatagccaga tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg
    11341 ctgccattct ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc
    11401 gtgcacaaca atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga
    11461 agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac
    11521 cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta
    11581 caaatgtacg gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga
    11641 tagttgagtc gatacttcgg cgatcaccgc ttccctcata ctcttccttt ttcaatatta
    11701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    11761 aaataaacaa atagctagct cactcggtcg ctacgctccg ggcgtgagac tgcggcgggc
    11821 gctgcggaca catacaaagt tacccacaga ttccgtggat aagcagggga ctaacatgtg
    11881 aggcaaaaca gcagggccgc gccggtggcg tttttccata ggctccgccc tcctgccaga
    11941 gttcacataa acagacgctt ttccggtgca tctgtgggag ccgtgaggct caaccatgaa
    12001 tctgacagta cgggcgaaac ccgacaggac ttaaagatcc ccaccgtttc cggcgggtcg
    12061 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
    12121 tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
    12181 aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
    12241 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
    12301 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
    12361 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
    12421 cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
    12481 ctccccaggt ggttttttcg aaggttaagt cagttgggga actgcttaac cttcgatcaa
    12541 accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg
    12601 atctcaagaa gatcctttga tcttttctac tgaaccgctc tagatttcag tgcaatttat
    12661 ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta
    12721 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
    12781 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
    12841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12901 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
    12961 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
    13021 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
    13081 gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc
    13141 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
    13201 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
    13261 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
    13321 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
    13381 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
    13441 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
    13501 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
    13561 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
    13621 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
    13681 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
    13741 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
    13801 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
    13861 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
    13921 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
    13981 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa
    14041 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt
    14101 ttaactttaa taaggagata taccatggtg agtcgtggct ttatgccgtt aggcgttgct
    14161 caagagaagt catttaataa ggccactgtt aaacgtaggt gagtcgtggc tttatgccgt
    14221 taggcgttgc tcaag
//
