LOCUS       sequence-33074-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP414
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..47)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaggatcccc gggataactt cgtatagcat acattatacg aagttatgcc atcctcatga
       61 aaactgtgta acataataac cgaagtgtcg aaaaggtggc accttgtcca attgaacacg
      121 ctcgatgaaa aaaataagat atatataagg ttaagtaaag cgtctgttag aaaggaagtt
      181 tttccttttt cttgctctct tgtcttttca tctactattt ccttcgtgta atacagggtc
      241 gtcagataca tagatacaat tctattaccc ccatccatac aatgccatct catttcgata
      301 ctgttcaact acacgccggc caagagaacc ctggtgacaa tgctcacaga tccagagctg
      361 taccaattta cgccaccact tcttatgttt tcgaaaactc taagcatggt tcgcaattgt
      421 ttggtctaga agttccaggt tacgtctatt cccgtttcca aaacccaacc agtaatgttt
      481 tggaagaaag aattgctgct ttagaaggtg gtgctgctgc tttggctgtt tcctccggtc
      541 aagccgctca aacccttgcc atccaaggtt tggcacacac tggtgacaac atcgtttcca
      601 cttcttactt atacggtggt acttataacc agttcaaaat ctcgttcaaa agatttggta
      661 tcgaggctag atttgttgaa ggtgacaatc cagaagaatt cgaaaaggtc tttgatgaaa
      721 gaaccaaggc tgtttatttg gaaaccattg gtaatccaaa gtacaatgtt ccggattttg
      781 aaaaaattgt tgcaattgct cacaaacacg gtattccagt tgtcgttgac aacacatttg
      841 gtgccggtgt ctcgcgcata
//
