LOCUS       sequence-330740-       12857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins (except TnsD) with crRNA 
            targeting PmcPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 12857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS20: pHelper(PmcCAST)_PmcPSP1_Delta-TnsD
FEATURES             Location/Qualifiers
     source          1..12857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(39..1121)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1083..1102
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1122..1199)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        1245..1263
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1264..1288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1549..1567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1568..1592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5491..5538
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5684..5702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5703..5727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9673..9691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9692..9716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      10784..10831
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10952..11743)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(11744..11835)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
       61 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      121 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
      181 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
      241 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
      301 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
      361 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
      421 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
      481 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
      541 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
      601 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
      661 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
      721 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
      781 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
      841 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
      901 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
      961 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     1021 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     1081 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     1141 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     1201 gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac gactcactat
     1261 aggggaattg tgagcggata acaattcccc tgtagaaata attttgttta actttaataa
     1321 ggagatatac catggtgagt cgtggcttta tgccgttagg cgttgctcaa gagaagtcat
     1381 ttaataaggc cactgttaaa cgtaggtgag tcgtggcttt atgccgttag gcgttgctca
     1441 agatccgaat tcgagctcgg cgcgcctgca ggtcgacaag cttgcggccg cataatgctt
     1501 aagtcgaaca gaaagtaatc gtattgtaca cggccgcata atcgaaatta atacgactca
     1561 ctatagggga attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag
     1621 aaggagatat acatatgaca gctatattaa ttcaaagtga ggaatatgta gacttaacat
     1681 ttaagctaag aggcgcaccg attcctcttg acaacggtta tcttacctac gctgccttat
     1741 cccgtatttg tcccccacta catgaactaa agtctattgg gattcatccg attgctggaa
     1801 taccgaccag gaacaatttg cttgagctaa ctgctcaatc tcgtctaaaa attcggattt
     1861 atcatcagca aattccttta atttatcctt atttagcggg tcaagctttt catataggtc
     1921 aaaattttta tcaactagat atccccgact acaaaccgtt aatttcttca gaaagtgtct
     1981 attccagatt agtgataatt aaagggttcc aagactctac taactttatt gaagctgtgc
     2041 aacggcaaat ggataattta ggaatacaag gaaaaattga acttcttacc cgacaagatg
     2101 gaacacctca acgaagacaa ttaaccatca ataaagaagg gaaacagttt aaagttagag
     2161 gatttggcgt aaaaatcagt gaactcaatc ctgaagattc cttaaccttg caagaacagg
     2221 gaattggcgg gaaacgaaag atgatgtgtg gaatatttgt cccagcgact cgcagcaagg
     2281 aagaagagga aacctgatga tagtctctac ccaacccaaa atttccctat ctctccatgc
     2341 tgcggatacg acaataatgc accgcgtcgg aatgaccgga ctttacatga ctttaaagcg
     2401 attggaaaag caatatccct tatctcgtca acggggagga catatatcat ggtttttgac
     2461 tgctgatact attgaattat tctgggaagg aagtgatttc atcgccttat cttggttaat
     2521 taatgagtct tttcaattag atgatacagg tttaattcat ttagtaggat tagacaatga
     2581 tcgaatagat ttaaggcaga aaatccatat tcatgaggga atatgcggtg tttttctacg
     2641 tcttaataag ttttatcaag caggagagat aattaacact gaactaaggt ttgaagaaaa
     2701 acaagtcgag tatcaatata aatccctaac ttggtacgca catcaaacct ttgcagagaa
     2761 gttatgcgag gcagataccc aacaactcag acatgattac attcaaataa ccagttggtt
     2821 gtatttaggg ggaattgttc gccatgctcg gacgcagaat actacaaaac tagaagagaa
     2881 acctgaatac gctttagcat tattatttgt accagtagtt tgtcattatt gcttacttca
     2941 tataccatca gaagacttaa aagaaaggaa accccatcgc tacttggtgg tgattccaga
     3001 aataaaggat tttgaagacg cttctcaaag gagatggcga ttacagcaat tagaaactaa
     3061 acagtttcat gttagtagcc ttggggaagc aggattactt tattacagct tagacgatat
     3121 tcagcctgaa gtggcctact atcaagcttg tcaagtttgg ttatatgaaa aaacaaataa
     3181 agcttctcgt caacggacat tgatgagtat agaagaaatt aaaattgata agaatatttt
     3241 aatcacttat caacaggtgc aaaaatactt taaaactaat tatcagataa ttaaatataa
     3301 gcaaattttt attaaggtca atccgattcg ctccctgatt gctgataatt tagtgaaagg
     3361 aatccattgg tggtctaatt tttgggaaaa attggtgata gaggattcaa aagaatattt
     3421 atttaaccag ttattttcca atcgagaagg attcatcata atggcagaga atagtgaaga
     3481 agacaagcag tatcttattt ttattaaggt atttcagcaa gctatgaagg gcaactttgc
     3541 taaaatttat gccaaaacag aggaaggtaa agatcctcca attaagaaga aagttgaacg
     3601 attaagggca gagctaaact attgttatga tgaattgtcg tttaaggaat atttgtctga
     3661 ctttttagta agagggggat taaataagta ttttaatgag catcaggaag agattgcact
     3721 actaattaaa aaatcacctt ggcaagaaat aagaatttgg tcattgttag cgatcgccag
     3781 ttataaaccc aaagataaac tcactaatag ggatgatagt tcattgtcaa acaatcaaaa
     3841 actagaagaa gtgaatgatg actcagaaga agaatgacag taatatccca aattattatc
     3901 tttacggaac agtcctcact cgttatggtt tagcttcctt aaatcatgac attagacgag
     3961 gaaataagac tattctgcaa aaaggctatt ggaataatgg taaaattcat tcttttgtag
     4021 gttcaagtgc aattcgttgg gcgttacgtt tttatcttca aaagcaaggt tatttagtta
     4081 atcgagtctg ggatgaggaa gaacatatta atcggttaac aagtgaagat ttcgatccag
     4141 aaaaatttta tgatgatgat atttttgggt ttgctttatt agagtctgct gaaacagagg
     4201 aggacacttc aacaacaaag agaaaaaaga aacaaacaaa gaccagcact cctaatcagc
     4261 gaatgggtgc tttagggatg aatatggctg tttcgctaac gccttatgat ggtgcagtta
     4321 agttaggggc aaaaagtggc agagagaagg atagtacatc tctccatttt acagaatatc
     4381 atgcaactcg atatcaatat tattttggaa ttgatgccac tcatctcaaa gatttttcgc
     4441 ggattttacc tatgatagat gggattatga atctgcccaa ggtaggaggg agtagtaata
     4501 tttttaatta tcctttctgt cccgatagtt tggtatttca atggacaaat cattttgctt
     4561 catatatttc ctattgcttt gaatattgcg atcctaagag taaggaagca aaactttcac
     4621 aagagtttat agatgaagtg gaatgcggac agattgaccc tagtaaattg tggattgggg
     4681 gaacaattgt taaggattta cagcaattgg ataattttga aagttcccct ttgaacaagg
     4741 cgcatattta tcgtaatcga aatgaaatga ttgaagcttt gaaaacagtc attaaaagag
     4801 atttgggctt ggaagaatca aaatgattgc accattaatt ctctatttgg atgttccatt
     4861 tacgactttt cgggagtccc atgcaaggga aatgggaaaa acctatcctg ttcctcctcc
     4921 agcgacagtg tatgggatgt tgttgtcttt ggtaggagaa acgaatgtct atcgccactg
     4981 tggggtagaa ttggccattg caatgttatc tagccccaaa aagtcgcgaa ttttgcgtca
     5041 aatgagacgg tttaagaatg ctgattttag ccatccagag aatgttattc cctgttatca
     5101 agaaattttg tctaatctga agtgtttgat ttgggttcgt tctgacgagg aaaagataca
     5161 accaagttta agggagagga tacagttagc gtttgaccat cctgagctag taagacggtt
     5221 tggttgttta tttttgggag aaagtgacca gttgattaaa acgatcaaac ttgcaaggga
     5281 agattatcta gagggagtga gacagtgggc gattagagat aatcgaggca ggttaacttt
     5341 accctattgg gttgaccatg tggggtcaag aaatacccga tttttgaggt atcggattga
     5401 ggaaatggat agattatcac ctcctgattt agcctggaca atggttcaat ctccgatttg
     5461 acctaggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     5521 gtcttgaggg gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt
     5581 ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctcg
     5641 ggatctcgac gctctccctt atgcgactcc tgcattagga aattaatacg actcactata
     5701 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
     5761 gagatatacc atgaaagagc aatttgaaca atgggttgct cgccttcaaa tatccgaggc
     5821 agggcaaaaa attattgaga aagttcgctc atctgagcca tcccgtcgcg ttggtggcgg
     5881 tagcaagaat gtttcgggac gctaccctag tcgcaagatg ggagtcacga ttcaatttga
     5941 gtctcatcgg gttgaactgc caattatcta tcagttagaa catgataaag atgttctaga
     6001 attctacgat caaccaccgc aaattaaact tgactatcaa gcaagtaacg gacgtagatt
     6061 aggcgtttta cacaccccag atttatttgt catccgaacc aattcggctg gttgggaaga
     6121 gtgtaaaact gagcaggatt tgaaaaaact agcagagaaa aaccccaatc gttactttta
     6181 ctctgaagaa aataatcaat ggcattcctt gccaggagaa gtttatgcta accagtttgg
     6241 gctttatcac cgcattcatt cagatcgcga aatcaattgg gttctgcaac gtaatttgca
     6301 atttttagaa gattattatc gctcagaatc tctagtagtg gaagaagcga tcgctcaatc
     6361 attacttgca attgtgtcat ctcaaccagg aatcacttta gcagaattac tcaatcggtc
     6421 aacaggagct aaatctgacg atatctacac cttaattatt caagagcaaa tctatattga
     6481 tttaaatgct acaccactag ctgaaccaga aagatgtcta atcttccgtg acgagcaaac
     6541 tgctagtgct tatagattaa tggttgagca accaagtgta agtcttccgg ctatctctcc
     6601 tgtagtcaat attgttactg gagcaactgt tagttgggac ggcaaaggtt taaatatcat
     6661 ccatgttgga gaaacagagg ttatacttgg cgcagaaaat aatcagttaa ttgaactcaa
     6721 aaaagctata tttgagaatc tggttcgcca aggaaaaatc acaagtctcc aaacaccaga
     6781 aaaaacagct attagcacag aatcttggca gcgatttcat caagctagtc ctgaagacca
     6841 agcagaagcc cttgaacgct acaaaactat cgaaccttac ttaaacggac atcctccaga
     6901 aaacgaaaca ataccagccc gtaccattcg tcactggaaa gctaaatatt taattgcaat
     6961 tcaaaaatac ggttgcggct acattgggct tttgtcccat cgcagtgtca aaggtaatcg
     7021 acagcgcaag ctgcccgaag acaccttagc aattatggag aagtttattt tagaagacta
     7081 tgaaactctt aaacaaaaaa gaatgtggga ggttcacgcg gctttagttc gagcttgcga
     7141 agaagctggt gtaattgctc cgagctataa agcatttacc aaagaagtac aacgccggac
     7201 tggttacgaa cagactaaaa aacgccaagg tcgtcgtgcc gcttatcagc atgaatcctt
     7261 ctattgggag cttgctataa ctaccccaag acacggcgat cgcccttttg aaattgggca
     7321 tatagatcat actgagttag atgtagagct agtttgctct gatacaggtc gcaatcttgg
     7381 cagaccttgg gcaacatttc tcgtagatgc ttattcacgg cgattgctgg cagtttacct
     7441 aacgtttgac tcaccctcat atcgttcttg cttaatggtg ctaagaattt gtgtcaagcg
     7501 tcatggcagg cttccccaaa ttgtagtggt agataacggt gcagagtttc acagcgttta
     7561 ctttgaaaca ctactagcta cttttgaatg tacaaaaaag caaagacccc cagccaaagc
     7621 tcgttttggt tcggtttgcg aacgcctatt tggaacttcc aatactcaat ttgtccataa
     7681 cttacttggt aatacacaaa tcactcgtaa tgtccgccaa gtcactaagt cagttaaccc
     7741 taaaaatcta gctgtatgga cacttggttt actttatgaa tatctttgtg cttgggctta
     7801 cgaagtttat gatacagacg aacatccggc acttttccag agtccgaggg atgcgtttgc
     7861 tgcgggtatg gcgattggag gcagtcgggt tcaccgcatg attccttacg atgaaaactt
     7921 tcagattctt accctaccta caacctcaga gggaaaagca aaagttcaag ttggacgcgg
     7981 cataaaaatt aactacatat actactggtc taatagtttc cgcgatcctc aaattgaaaa
     8041 tacctctgtg cagataagat acgacccgtt taatattggc atcgcttacg cctttgttcg
     8101 aggacagtgg gtacagtgta tctctcaata ttatgcagaa ctccaaggtc gttcagaaaa
     8161 agaactgaag ttagctagta tcgagttacg caaacactat tcaaatcata cacaacaatc
     8221 taaagtttcg gctaagaatt tagcagaatt tttagcatcg gtagaagctc aagaagctct
     8281 attagaacag cgttcctatg atgcagaagt taaggaagtc tttagggtaa tcgagggtgg
     8341 aaaagcgaca acaagccgta acgaagaacc aaaacttatt caagttactt ttgccaatac
     8401 cgattttcaa gcggatgaag atgaagcgat cgtcccagaa acacttgtag tgtatgagga
     8461 gttttaacta tgacaaaatc tactggattt ccgcttgaac tcctcacacg gcctgctaca
     8521 gaaaggcttg cttactttga aaattacacc gtagcgcatc ctcgacttaa agaagtctat
     8581 gaaattctca tgcgaacgat tgccgaacca gcaggagctt catttatctt tgtttacggt
     8641 gctagtggtg ttggtaaaac aactttgcga ctccgcgttg agcagaaatt aacagaacta
     8701 gcattaccta aattggaaag cgatcgcgct cgtgttcctg ttgttggtat tgaagctata
     8761 gcgccagaat cgagatactt caactggaaa gaatactaca cacgcgcctt aattactttg
     8821 gaagaaccat taatcgatca caagtttgat tacggtgtgc gtggtatctc tcgcgataat
     8881 tttggcaaaa ttaatgttga gtcgaaagta gttgcacctg ctctacgcag agctttagaa
     8941 aatgctctca tccatcgtca tccagacgta ttttttgtag atgaagcaca acattttggt
     9001 aaggtggcaa gcggatacaa actacaagat caattagact gtctcaaatc tttagcaaat
     9061 atgacaggca tcttgcactg tctattagga acatacgaac tacttacatt ccgtaatttg
     9121 agcggtcaac tctcacgtcg tagtgttgat attcatttcc gccgttattg tgctgatagc
     9181 ccagaagatg tacaggcttt taagagtgtg ttgttaactt ttcaacagca tctgccacta
     9241 gcagaaactc caaatttagt cgatcattgg gaatattttt acgaacgtac tttaggatgt
     9301 attggcacac tgaaagattg gctgaagcgt gttcttagtg atgctttgga tcgagaagca
     9361 acaacaatca ctctcaaaga tttacaaaaa cgtgctttgt cggttgctca gtgtcagaaa
     9421 atgttcaagg aaattcaaga gggtgaacga caattatctg aaacagaagc agatgtgcaa
     9481 aacttacgct ctgcattagg acttggcgca aaaccaattg tgctacccga agaaacccca
     9541 aaaactactc gtccgccagg aaaggttggc aaacgcaaac ctaaaagaga tcccatcgga
     9601 gtgcagcaag atgttagcta gcttaagtcg aacagaaagt aatcgtattg tacacggccg
     9661 cataatcgaa attaatacga ctcactatag gggaattgtg agcggataac aattccccat
     9721 cttagtatat tagttaagta taagaaggag atatacatga ctatttactc agaacattgg
     9781 agcctaaatc ctctagaaat cccgcaacgg agtcggttat tttctcttga acctgtggca
     9841 gtgggaactc cctatgcaga aagtcttagt agctatctcc atcgtttagc gcaggcgcat
     9901 tgtttaacct ctgagaagct cgttatggga gaaattgcgc cacttattct caaagatgag
     9961 gataaatctg agctattgtc aaaaaatctg agtcacctat tgggaaatag cgacgctaaa
    10021 ccggctatta acggaatgcg agaaatgaca gaaaagttag tcactgtctt agaagagtta
    10081 acaatgcgtc aggatttacg ctttttgacc cttttaagct ggaaaggaat gatttatgac
    10141 aagggattat ttcggaacta tcgcgcttgg tgtccttgtt gttgtgaaga gtggatgcag
    10201 aagaacaaaa ctatctatga accattatca tggtcattta aggacgtaga attttgtttg
    10261 attcacaaac agcgattaat agaagaatgt tctcattgtg gtgcccgttt acctgtgatg
    10321 gctaggcttt cacctgcggg attttgttct cgttgttacg ggtggttagg acaggaaatt
    10381 aaaggcgaag aagaaataga gaaatatcga gttaatattc aaggaattag cgaattaatt
    10441 gcacttactc cccaattagg ttataaaccc atcccgatcg agttaacccg aaaattacaa
    10501 ttgattttgt tagtgtttga acaagcaatt ggaaaggatg tgaagttatt gggagattta
    10561 ggaggaatca tggaatcgtt gaggatagcc tcaacaacaa atcaaagtca accttatcat
    10621 ttggtgaagt tgattattcc tgtgtgcgag aaggcgaaga ttagtgtatt ccagttgttt
    10681 gggtcagatt ttaaggaatt gggcaagata ttgttcggga attttagtct ggaattaaag
    10741 ttatagttaa ttaacctagg ctgctgccac cgctgagcaa taactagcat aaccccttgg
    10801 ggcctctaaa cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac
    10861 ggtcacactg cttccggtag tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg
    10921 cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
    10981 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
    11041 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
    11101 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
    11161 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
    11221 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
    11281 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
    11341 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
    11401 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
    11461 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
    11521 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
    11581 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
    11641 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
    11701 ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat
    11761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11821 agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg agactgcggc
    11881 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
    11941 tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc
    12001 cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga ggctcaacca
    12061 tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggcgg
    12121 gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc
    12181 tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg
    12241 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc
    12301 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg taaaacgcca
    12361 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
    12421 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
    12481 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
    12541 ccacctcccc aggtggtttt ttcgaaggtt aagtcagttg gggaactgct taaccttcga
    12601 tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa
    12661 aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt tcagtgcaat
    12721 ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg taattctcat
    12781 gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
    12841 cggtcgagat cccggtg
//
