LOCUS       sequence-330742-       10408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CodA-based vector for insertion of DNA by recombination downstream 
            of the pyrE gene in C. difficile.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Fuchs M, Lamm-Schmidt V, Sulzer J, Ponath F, Jenniches L, Kirk JA, 
            Fagan RP, Barquist L, Vogel J, Faber F
  TITLE     An RNA-centric global view of Clostridioides difficile reveals broad
            activity of Hfq in a clinically important gram-positive bacterium.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 22;118(25). pii: 2103579118. doi:
            10.1073/pnas.2103579118.
  PUBMED    34131082
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-22"; volume: "118(25). pii: 
            2103579118. doi"; pages: " 10.1073/pnas.2103579118"
COMMENT     Sequence Label: pJAK080
FEATURES             Location/Qualifiers
     source          1..10408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      125..713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     614..633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     867..884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            1034..1143
                     /note="incP origin of transfer"
     CDS             1176..1547
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     primer_bind     1744..1760
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1744..1760
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1935..1959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1978..1986
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2002..3282
                     /codon_start=1
                     /gene="codA"
                     /product="E. coli cytosine deaminase"
                     /label=codA
                     /note="confers sensitivity to 5-fluorocytosine"
                     /translation="SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENS
                     LDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRA
                     WQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYP
                     NGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQ
                     SRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIH
                     LQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQL
                     MGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVR
                     GGKVIASTQPAQTTVYLEQPEAIDYKR"
     primer_bind     complement(3382..3399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3382..3398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3391..3413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(7272..7898)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     7803..7822
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    8005..8023
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 attgatttta aattttatta atgttatata ctatcctttt tgataatctc atgaccaaaa
       61 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      901 caatacgcag ggccccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct
      961 ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc
     1021 aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct
     1081 tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc
     1141 cggctaccgc cggcgtaaca gatgagggca agcggatggc tgatgaaacc aagccaacca
     1201 ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga gcgattgagg
     1261 aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc ggccagggct
     1321 acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc atcaatggcg
     1381 acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg cgcacggcgc
     1441 ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag caggacgagc
     1501 ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt ttttagccgc
     1561 taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc catcaagaag
     1621 agcgacttcg cggagctggt gaagtacatc accgacgagc aaggcaagac cgatcgggcc
     1681 ccctgcagga taaaaaaatt gtagataaat tttataaaat agttttatct acaatttttt
     1741 tatcaggaaa cagctatgac cgcggccgct gtatccatat gaccatgatt acgaattccc
     1801 cggatcgaga tagtatatga tgcatattct ttaaatatag ataaagttat agaagcaata
     1861 gaagatttag gatttactgt aatataaatt acacttttaa aaagtttaaa aacatgatac
     1921 aataagttat ggttggaatt gttatccgct cacaattcca acttatgatt aaaattttaa
     1981 ggaggtgtat ttcataatgt gtcgaataac gctttacaaa caattattaa cgcccggtta
     2041 ccaggcgaag aggggctgtg gcagattcat ctgcaggacg gaaaaatcag cgccattgat
     2101 gcgcaatccg gcgtgatgcc cataactgaa aacagcctgg atgccgaaca aggtttagtt
     2161 ataccgccgt ttgtggagcc acatattcac ctggacacca cgcaaaccgc cggacaaccg
     2221 aactggaatc agtccggcac gctgtttgaa ggcattgaac gctgggccga gcgcaaagcg
     2281 ttattaaccc atgacgatgt gaaacaacgc gcatggcaaa cgctgaaatg gcagattgcc
     2341 aacggcattc agcatgtgcg tacccatgtc gatgtttcgg atgcaacgct aactgcgctg
     2401 aaagcaatgc tggaagtgaa gcaggaagtc gcgccgtgga ttgatctgca aatcgtcgcc
     2461 ttccctcagg aagggatttt gtcgtatccc aacggtgaag cgttgctgga agaggcgtta
     2521 cgcttagggg cagatgtagt gggggcgatt ccgcattttg aatttacccg tgaatacggc
     2581 gtggagtcgc tgcataaaac cttcgccctg gcgcaaaaat acgaccgtct catcgacgtt
     2641 cactgtgatg agatcgatga cgagcagtcg cgctttgtcg aaaccgttgc tgccctggcg
     2701 caccatgaag gcatgggcgc acgagtcacc gccagccaca ccacggcaat gcactcctat
     2761 aacggggcgt atacctcacg cctgttccgc ttgctgaaaa tgtccggtat taactttgtc
     2821 gccaacccgc tggtcaatat tcatctgcaa ggacgtttcg atacgtatcc aaaacgtcgc
     2881 ggcatcacgc gcgttaaaga gatgctggag tccggcatta acgtctgctt tggtcacgat
     2941 gatgtcttcg atccgtggta tccgctggga acggcgaata tgctgcaagt gctgcatatg
     3001 gggctgcatg tttgccagtt gatgggctac gggcagatta acgatggcct gaatttaatc
     3061 acccaccaca gcgcaaggac gttgaatttg caggattacg gcattgccgc cggaaacagc
     3121 gccaacctga ttatcctgcc ggctgaaaat gggtttgatg cgctgcgccg tcaggttccg
     3181 gtacgttatt cggtacgtgg cggcaaggtg attgccagca cacaaccggc acaaaccacc
     3241 gtatatctgg agcagccaga agccatcgat tacaaacgtt gaacgactgg gttacagcga
     3301 gcttagttta aagggcgaat tcctagagtc gacgtcacgc gtccatggag atctcgaggc
     3361 ctgcagacat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     3541 ctagcataaa aataagaagc ctgcatttgc aggcttctta tttttatggc gcgccgccat
     3601 tatttttttg aacaattgac aattcatttc ttatttttta ttaagtgata gtcaaaaggc
     3661 ataacagtgc tgaatagaaa gaaatttaca gaaaagaaaa ttatagaatt tagtatgatt
     3721 aattatactc atttatgaat gtttaattga atacaaaaaa aaatacttgt tatgtattca
     3781 attacgggtt aaaatataga caagttgaaa aatttaataa aaaaataagt cctcagctct
     3841 tatatattaa gctaccaact tagtatataa gccaaaactt aaatgtgcta ccaacacatc
     3901 aagccgttag agaactctat ctatagcaat atttcaaatg taccgacata caagagaaac
     3961 attaactata tatattcaat ttatgagatt atcttaacag atataaatgt aaattgcaat
     4021 aagtaagatt tagaagttta tagcctttgt gtattggaag cagtacgcaa aggctttttt
     4081 atttgataaa aattagaagt atatttattt tttcataatt aatttatgaa aatgaaaggg
     4141 ggtgagcaaa gtgacagagg aaagcagtat cttatcaaat aacaaggtat tagcaatatc
     4201 attattgact ttagcagtaa acattatgac ttttatagtg cttgtagcta agtagtacga
     4261 aagggggagc tttaaaaagc tccttggaat acatagaatt cataaattaa tttatgaaaa
     4321 gaagggcgta tatgaaaact tgtaaaaatt gcaaagagtt tattaaagat actgaaatat
     4381 gcaaaataca ttcgttgatg attcatgata aaacagtagc aacctattgc agtaaataca
     4441 atgagtcaag atgtttacat aaagggaaag tccaatgtat taattgttca aagatgaacc
     4501 gatatggatg gtgtgccata aaaatgagat gttttacaga ggaagaacag aaaaaagaac
     4561 gtacatgcat taaatattat gcaaggagct ttaaaaaagc tcatgtaaag aagagtaaaa
     4621 agaaaaaata atttatttat taatttaata ttgagagtgc cgacacagta tgcactaaaa
     4681 aatatatctg tggtgtagtg agccgataca aaaggatagt cactcgcatt ttcataatac
     4741 atcttatgtt atgattatgt gtcggtggga cttcacgacg aaaacccaca ataaaaaaag
     4801 agttcggggt agggttaagc atagttgagg caactaaaca atcaagctag gatatgcagt
     4861 agcagaccgt aaggtcgttg tttaggtgtg ttgtaataca tacgctatta agatgtaaaa
     4921 atacggatac caatgaaggg aaaagtataa tttttggatg tagtttgttt gttcatctat
     4981 gggcaaacta cgtccaaagc cgtttccaaa tctgctaaaa agtatatcct ttctaaaatc
     5041 aaagtcaagt atgaaatcat aaataaagtt taattttgaa gttattatga tattatgttt
     5101 ttctattaaa ataaattaag tatatagaat agtttaataa tagtatatac ttaatgtgat
     5161 aagtgtctga cagtgtcaca gaaaggatga ttgttatgga ttataagcgg ccggccagtg
     5221 ggcaagttga aaaattcaca aaaatgtggt ataatatctt tgttcattag agcgataaac
     5281 ttgaatttga gagggaactt agatggtatt tgaaaaaatt gataaaaata gttggaacag
     5341 aaaagagtat tttgaccact actttgcaag tgtaccttgt acctacagca tgaccgttaa
     5401 agtggatatc acacaaataa aggaaaaggg aatgaaacta tatcctgcaa tgctttatta
     5461 tattgcaatg attgtaaacc gccattcaga gtttaggacg gcaatcaatc aagatggtga
     5521 attggggata tatgatgaga tgataccaag ctatacaata tttcacaatg atactgaaac
     5581 attttccagc ctttggactg agtgtaagtc tgactttaaa tcatttttag cagattatga
     5641 aagtgatacg caacggtatg gaaacaatca tagaatggaa ggaaagccaa atgctccgga
     5701 aaacattttt aatgtatcta tgataccgtg gtcaaccttc gatggcttta atctgaattt
     5761 gcagaaagga tatgattatt tgattcctat ttttactatg gggaaatatt ataaagaaga
     5821 taacaaaatt atacttcctt tggcaattca agttcatcac gcagtatgtg acggatttca
     5881 catttgccgt tttgtaaacg aattgcagga attgataaat agttaacttc aggtttgtct
     5941 gtaactaaaa acaagtattt aagcaaaaac atcgtagaaa tacggtgttt tttgttaccc
     6001 taaatatatc ttgcccaaat gtcaaagctg gtggaatggc ttttggtata aaaaatgaag
     6061 tggcaagaga agttgttcgt gaggtaagaa acataacgaa actgccttta gtaataaaat
     6121 tatcacccaa tgcagaagat attgtaggaa tggcaaaagt ttgtgaagaa gaaggtgctg
     6181 atggagtttc attagttaat acttttaaag caatggcaat tgatataaaa aatagaagac
     6241 cagtatttga aaatgtatat gctgggttat ctggaccagc tataaaacct atagctttga
     6301 gaatggtaca tgaagtgtgt aaaaatgtaa atatacctgt aatggggatg ggcggaataa
     6361 ctaaagccac agatgctata gagtttataa tggcaggagc tacttgtatc caagttggaa
     6421 ctgcaaattt tattaatcct agaataggta ttgaaataat agatggaatt aatgaattta
     6481 tggatagaga aggaataaaa agtttagacg aaataagagg aattatataa ggtagaataa
     6541 atagtatttt aaagataaaa aatatagatt aaataaacaa taattttata acattaacat
     6601 ggaggaaaaa taatatgagt aaagtagatg tagtggatat attaaagaaa agtgatgcat
     6661 tgttggaggg acatttttta ttatcttcag gaaaacacag taatagatac gtacaatgtg
     6721 caaaagtatt aagattccca gaatatgctg ctgaggtatt aagtacagtt gttgaacaaa
     6781 taaaagactt agatatagac ttagtagtag gaccagctat gggtggagta atagtttctt
     6841 atgagttagg aagacaatta ggaaaagaag ctgtatttac tgagagaaaa gacaatacaa
     6901 tggagttaag aagaggattt gaagttaaaa aaggagcaaa gataataatt gctgaagacg
     6961 ttgtaactac tggtaaatca actatggaga caaaaagagt attagaagcc ttaggtggag
     7021 aagttgtagg tgttgcatgt atagcagata gaactaatca tgatataggt atgcctatat
     7081 atagtgctat aaaacttgat attcaagttt atgaatctga tgagtgtcct ttatgtaagg
     7141 aaggaaaatt accagttgtt aagcctggaa gtagagagtt caaggaatta gggatgtaat
     7201 aacgaattct gcatcaagct agcataaaaa taagaagcct gcatttgcag gcttcttatt
     7261 tttatggtac cttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc
     7321 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct
     7381 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact
     7441 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc gctgagtgca
     7501 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga
     7561 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga
     7621 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc
     7681 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg
     7741 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc
     7801 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg
     7861 ctgttaatca ctttactttt atctaatcta gacatcatta attcctcctt tttgttgaca
     7921 ttatatcatt gatagagtta tttgtcaaac tagtttttta tttcgatgcc ctggacttca
     7981 tgaaaaacta aaaaaaatat tgacactcta tcattgatag agtataatta aaataagctt
     8041 gatcgtagcg ttaacagatc tgagctcaga atgttttaaa catgattctt aatagtagga
     8101 aggagtaaaa tcatggctaa ggaaaaaaag aaagaaaaaa aaggtttttt tagttttaat
     8161 aagatgacaa aggatatggg aatagattta gggactgcaa atacattagt ttacataaaa
     8221 ggacaaggta ttgttgttag agaaccatca gttgtcgcaa taagagatga tagtaaagag
     8281 gtattagcag taggtgaaga agccaaaaaa atgataggta gaacaccagg aaatatagta
     8341 gctataagac ctatgaaaga tggagtaata gctgattttg acataactca atcaatgatt
     8401 agctatttta tacaaaaagc ggctgataaa aaaggtgttg taagtccaag aatagctata
     8461 tgtgttcctt ttggagttac agaagtagaa aagagagcta tagaagaggc tgcaagacaa
     8521 gcaggagcta aagatgcatt ccttatagaa gagccaatgg cagcggctat aggggctgga
     8581 ttaaaagttg aagaaccaga aggtaatatg gtagttgata ttggtggagg tacttcagaa
     8641 atagctgtaa tatctttagg aggaatcgtt actgctaaat ctataagaat aggtggagac
     8701 gaatttgacg agtctatagt ggcttatgta aaaaaagaat ataatcttat gataggtgaa
     8761 agaactgctg aaaatgtaaa aattaacata ggttctacat ttaaagatga tgaagaaata
     8821 aatatgcaaa taagaggtag agatttaatc tcaggattac ctaaaacaat agaaatatgc
     8881 tctacagaag ttagagaagc attaaaagaa cctgtaagtt caatagtaga tgcaataaaa
     8941 tctacactag aaagaactcc accagaatta gcctcagata taatggaaaa cggaataatg
     9001 ctaacaggag gaggcgcttt gcttagaggt cttgataagc ttattacaca ggaaactggt
     9061 atgtctgtac aaatagcaga aacacctctt gattgtgttg cacttggaac aggtaaatct
     9121 gttgaagacc aagaaatctt tgaaaaagta ttaatgatga atactaagaa ttagggatcc
     9181 tataagtttt aataaaactt taaatagaaa aaggcttctc tcatgagaag tcttttttat
     9241 ttaaacttaa ttatttatag tgttacttaa aaaatgtaaa ttttttagag taaaaatttg
     9301 taatataaca tataacttaa aaataaaaag cagttagcct tataaatgaa ggttagctgt
     9361 tttttttgtt aaaaatatat ttacaaaata agagatagta gcagtacaat ataagtataa
     9421 ggtaaaagta ttatatatta gggaggggta agtatgaaaa atcaattaaa tgaaaataat
     9481 aaaaaggttg tattttttgg agtaggtgct gtaggagcta cttttgcaga acaatttttt
     9541 aactctaaat atgattttaa aattctttgt gataatgaaa gaaagaaaag atatttagaa
     9601 gaaggattta taataaatgg aaaaaggtat gattttgatt atgtaactaa agatgagtat
     9661 aaacaagagg ctgattttat aattataggt ctaaaatata ataatttaaa agaaaatata
     9721 aaagaattag atggattagt tggaaaaaat acagttataa tgtctctgct aaatggagtt
     9781 gatagcgaag agataatagg agaaagattt ggaattgaaa aaatggtata ttcatatgtt
     9841 accaatatag atgcaaagaa aatcaataat aatattatac acactactaa tgggataatt
     9901 gtatttggta acaaagataa tagtgaagat agaaaaacta atataataac cgaagtcttt
     9961 gatgatgtaa atatagaata tactttatca aaagatattc aacgagatat gtggtggaag
    10021 tacatggtta atattggtgt aaatcaaact tcagctatac ttggtgcacc ttatggagtt
    10081 tttcagagtt ctgagcattt aagagaatta gcaaaatctg caatgaggga agttgttgct
    10141 atagcacaag caaaagacat atctcttaca gaagatgatg tggaacattc attacataga
    10201 atactagaac attcaaaaga aggaagaaca tcaatgcttc aagatgtgga agctcataga
    10261 cttacagaag tagatatgtt ttctaagaat atctgtaaac ttggaaaaaa atataacata
    10321 cctactccta taaatcagac tttcttttat atgataaaag taattgaaag tagattttaa
    10381 aggggctttg taagaaatat tgataatt
//
