LOCUS       sequence-33075-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP416
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        58..339
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttataac
       61 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
      121 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
      181 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat
      241 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg
      301 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca
      361 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt
      421 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca
      481 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca
      541 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct
      601 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa
      661 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac
      721 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa
      781 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa
      841 gagagccccg aaagcttaca ttttatgtt
//
