LOCUS       sequence-330763-       10041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: APT3-VAMP4
FEATURES             Location/Qualifiers
     source          1..10041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1355..1807
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4661..5641)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5705..6784)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6784..8058)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     promoter        complement(8239..8257)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      8821..9043
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9091..10041
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaaaatggct cgtgtacagt ttaaacaacg
     1381 tgaatctact gacgcaatct ttgttcactg ctcggctacc aagccaagtc agaatgttgg
     1441 tgtccgtgag attcgccagt ggcacaaaga gcagggttgg ctcgatgtgg gataccactt
     1501 tatcatcaag cgagacggta ctgtggaggc aggacgagat gagatggctg taggctctca
     1561 cgctaagggt tacaaccaca actctatcgg cgtctgcctt gttggtggta tcgacgataa
     1621 aggtaagttc gacgctaact ttacgccagc ccaaatgcaa tcccttcgct cactgcttgt
     1681 cacactgctg gctaagtacg aaggcgctgt gcttcgcgcc catcatgagg tggcgccgaa
     1741 ggcttcccct tcgttcgacc ttaagcgttg gtgggagaag aacgaactgg tcacatctga
     1801 ccgtggtagc ggcggtggtg cgagtggtgg tgcgattgag cgtggagaac gccttgacga
     1861 attgcaagac aaatcggagt ctcttggcgg tagcgcaggc agtggagcgg gcggtaacac
     1921 gattaacatc gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac
     1981 tctggctgac cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc
     2041 ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga
     2101 ggttgcggat aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc
     2161 acgcatcaac gactggtttg aggaagtgaa agctaagctc ggcaggcgcc cgacagcctt
     2221 ccagttcctg caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct
     2281 ggcttgccta accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg
     2341 ggccattgag gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa
     2401 gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat
     2461 gcaagttgtc gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc
     2521 gtggtataag aaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattaagtc
     2581 aactggaatg gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac
     2641 tatcgaactc gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg
     2701 catctctccg atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg
     2761 tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa
     2821 agcactgatg cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc
     2881 gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa
     2941 gtggaagcat tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa
     3001 accggaagac atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc
     3061 tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc agacttgagt tcatgcttga
     3121 gcaagccaat aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg
     3181 cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact
     3241 gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca
     3301 cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga
     3361 ggaaaaccac gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc
     3421 tgagcaagat tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca
     3481 ccacggcctg agctataact gctcccttcc gctggcgttt gacgggtctt gctctgtcat
     3541 ccagcacttc tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc
     3601 tagtgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca
     3661 agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg
     3721 tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta
     3781 cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga
     3841 gttcggcttc cgtcaacaag tgctggacga taccattcag ccagctattg attccggcaa
     3901 gggtctgatg ttcacccagc cgaatcaggc tgctagatac atggctaagc tgatttggga
     3961 atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc
     4021 taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg
     4081 cgctgtgcat tggataactc ctgatggttt ccctgtgtgg caggaataca agaagcctat
     4141 tcagacgcgc ttggacctga tgttcctcgg tcagttccgc ttacagctta ccattaacac
     4201 caacaaagat agcgagattg atgcacacaa acaggaggct ggtatcgctc ctaactttgt
     4261 acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg
     4321 aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa
     4381 cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc
     4441 tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc
     4501 acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc
     4561 gtaatggaga ttttcaacat gacgccatgg gcattaaaaa atgccctctt gggttatcaa
     4621 gagggtcatt atatttgctg aggagtgccg ttaattaagt ttaggtatat tccgtgtggt
     4681 acttcttaat attatcatca acaatattga ttacattttt ttggctcatc aaatcattca
     4741 ttggttcaaa ggacagcaat acacttttcg caccacactt ttcaattgcc aacttagccg
     4801 cagttataca ctccgtataa tttccgacag cgttttctgc aattatttct tcaagtttat
     4861 tttcgaaatt ttcattaggg tgcatttcaa gaacataatc actaataaat gcacgcgtct
     4921 cttgtttagc tttattacta tcttcgttat agttaactaa tatcattaac tgatggtcta
     4981 tctctgatag gtcaacgtca tatttatccg caacggcttt atatctttca gcatattcat
     5041 atctaacatc attagaatca tcccacttaa agatgagagg aatacctttt ttggccgccc
     5101 actcaacaat atgatgactg gttgctgtta catatttccg aggtccgcct ggcgtataag
     5161 catggggatt tacagatatt ttagggaagc tataaaaatc gttatctgga ttacaatagc
     5221 ctgttgttaa agcatcgtta atgatttcat aacactcttc aaatagttgc tgttgatatt
     5281 caaccgggcg attaaaaaaa tgcatttcat cttttttttc gcaatcacta aaccctaaaa
     5341 taaatctccc ttcacttaac tgatccaata agcaagcttc ctccgctatg cggacaggat
     5401 gatgagttgt aatgatgtga tttaatgaac caattttaat tttctctgtt aaaccgagca
     5461 gaaaaccaga aacagtcaga ggagcgccga caacaccatt atctgaaaaa tgattttcat
     5521 acactaaaat ctgttcaaaa ttcaacttat caacatactc cgttatttcc tgcatgcgaa
     5581 ctatactctg ttcttgaaca gttgttgaat tgatgaagtt aaggaagaac aatccaaatt
     5641 tgctaccgcc accgccgcta ccgccaccgc cgctaccgcc accgccgcta ccgccaccgc
     5701 cataatataa tagcgaacgt tgtttttctt taagaaatgg catgacatca gactggaaga
     5761 gcttcatgga agcaataatt tcgtctactg ttccattagc ttcaaatcca caacaaatat
     5821 ttgatattcc tgtagcatca atgtcttttt gaattatgtc aatacattcc tgcggcgttc
     5881 ccacgggatt gatttcgtaa ctgtaatcaa tacggcgatt agtatcttta tgtcctttta
     5941 atacaaagtc acgccactgc cctttattga aatcataacc tcttgtttgg tctgaatcat
     6001 caaaaatagt cgtagcattc acataagaat cataccaatg ccccagaaat ttccggcaaa
     6061 tctctttcgc tttaattgag tcatgatcta cagatgttat atatgataag caatggtcga
     6121 tattatgaat atcgtgccca tattcttgag ccacttcatt ataaagctca agttgtgctt
     6181 tcttttcgtt agtatttata atccaactta atatcatcgg taggccaaat tgagcagccc
     6241 actcagtcgt cgaagctgat tcagccacca cataaaccgg tgcgccacct ctgctatacg
     6301 ccgcggggtt tacttttacc ttatggaact tgatatgttc attatcagct tccatatatc
     6361 cctctgtcat gccattcttt atcagcccgt accagcattc cgctaaggcg cgactgttat
     6421 tcatatctgt gccgaatacg cgaaagtcct tgttgtaaag ccctcggcaa ataccaaacc
     6481 gaaatcgtcc ttttgacatt tgatccaata aattcacatc ttcaagttgg cgtactggat
     6541 gggctgtggg aagaacaata gcggcagttc ctacattcaa ttttttagtc gcgccaagta
     6601 aatatgcagc agcgacataa gggttaccaa gcaaaccaaa ctccgtgaaa tgatgctcca
     6661 gtaaccatac ggtatcaaaa ccacactcct cagagatgcg acctaattta accaaacgtt
     6721 tcattacctc tgtttgggaa aattggggag gttggtatgt aagcaaaaag tttccaaatt
     6781 tcattaagac tccttattac gcagtatgtt agcaaacgta gaaaatacat acataaaggt
     6841 ggcaacatat aaaagaaacg caaagacacc gcggaacagg ttgatcttat cgcagtcgat
     6901 actgaactcg taaggtttac cagcgccaaa gacaaaaggg cgacattcaa ccgattgagg
     6961 gagggaaggt aaatattgac ggaaattatt cattaaaggt gaattatcac cgtcaccgac
     7021 ttgagccatt tgggaattag agccagcaaa atcaccagta gcaccattac cattagcaag
     7081 gccggaaacg tcaccaatga aaccatcgat agcagcaccg taatcagtag cgacagaatc
     7141 aagtttgcct ttagcgtcag actgtagcgc gttttcatcg gcattttcgg tcatagcccc
     7201 cttattagcg tttgccatct tttcataatc aaaatcaccg gaaccagagc caccaccgga
     7261 accgcctccc tcagagccgc caccctcaga accgccaccc tcagagccac caccctcaga
     7321 gccgccacca gaaccaccac cagagccgcc gccagcattg acaggaggtt gaggcaggtc
     7381 agacgattgg ccttgatatt cacaaacgaa tggatcctca ttaaagccag aatggaaagc
     7441 gcagtctctg aatttaccgt tccagtaagc gtcatacatg gcttttgatg atacaggagt
     7501 gtactggtaa taagttttaa cggggtcagt gccttgagta acagtgcccg tataaacagt
     7561 taatgccccc tgcctatttc ggaacctatt attctgaaac atgaaagtat taagaggctg
     7621 agactcctca agagaaggat taggattagc ggggttttgc tcagtaccag gcggataagt
     7681 gccgtcgaga gggttgatat aagtatagcc cggaataggt gtatcaccgt actcaggagg
     7741 tttagtaccg ccaccctcag aaccgccacc ctcagaaccg ccaccctcag agccaccacc
     7801 ctcattttca gggatagcaa gcccaatagg aacccatgta ccgtaacact gagtttcgtc
     7861 accagtacaa actacaacgc ctgtagcatt ccacagacag ccctcatagt tagcgtaacg
     7921 atctaaagtt ttgtcgtctt tccagacgtt agtaaatgaa ttttctgtat ggggttttgc
     7981 taaacaactt tcaacagttt cagcggagtg agaatagaaa ggaacaacta aaggaattgc
     8041 gaataataat tttttcattt tttccttttt actgcacctg caggtctttc ttatgagtta
     8101 cctcctgaac ttggtaaaca gaacaacaaa gctgcctgat agtggccttt atttttggca
     8161 taaataacag aataaacact gcactgtgta ttcattccaa cgagtgaata cacggagcaa
     8221 tgtcgctcct tctttctccc tttagtgagg gttaattgta gggtccctaa gtctcctcag
     8281 caaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaccg gatgtcctct
     8341 tgacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg cattagccag
     8401 tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca gagggcagga
     8461 actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata aaacgccctg
     8521 agaagcccgt gacgggcttt tcttgtatta tgggtagttt ccttgcatga atccataaaa
     8581 ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca gcgaactgaa
     8641 tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc
     8701 gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg tctgttttgt
     8761 agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga ctaaagtagt
     8821 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
     8881 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
     8941 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
     9001 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
     9061 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
     9121 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
     9181 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
     9241 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
     9301 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
     9361 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
     9421 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
     9481 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
     9541 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
     9601 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
     9661 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
     9721 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
     9781 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     9841 acatcagatt cctacctacg taacggacta agaaaaacac tacacgatgc tttaactgca
     9901 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagcatgat
     9961 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
    10021 gctaaatacg gaaggatctg a
//
