LOCUS       sequence-33078-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP422
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..54
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gactctagag gatccccggg ataacttcgt ataatgtatg ctatacgaag ttatggattt
       61 tcttaacttc ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat
      121 caccagttct gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt
      181 cttcaggcaa gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag
      241 ggttgacctt attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa
      301 atgcggtgtt cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac
      361 ctgggataac ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa
      421 taccatttag gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat
      481 gttgaacctt caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata
      541 atcttgaaga ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat
      601 gttgtagggc catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt
      661 gttcactatc ccaagcgaca ccatcaccat catcttcctt tctcttacca aagtaaatac
      721 ctcccactaa ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg
      781 gagataagtc taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt
      841 gaagttcttt acggattttt agtaaacct
//
