LOCUS       sequence-330782-       10109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: AP-VAMP1
FEATURES             Location/Qualifiers
     source          1..10109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1353..1802
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDR"
     CDS             complement(4728..5708)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5772..6851)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6851..8125)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     primer_bind     complement(8306..8325)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8307..8325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8889..9111
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9159..10109
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaatggctcg tgtacagttt aaacaacgtg
     1381 aatctactga cgcaatcttt gttcactgct cggctaccaa gccaagtcag aatgttggtg
     1441 tccgtgagat tcgccagtgg cacaaagagc agggttggct cgatgtggga taccacttta
     1501 tcatcaagcg agacggtact gtggaggcag gacgagatga gatggctgta ggctctcacg
     1561 ctaagggtta caaccacaac tctatcggcg tctgccttgt tggtggtatc gacgataaag
     1621 gtaagttcga cgctaacttt acgccagccc aaatgcaatc ccttcgctca ctgcttgtca
     1681 cactgctggc taagtacgaa ggcgctgtgc ttcgcgccca tcatgaggtg gcgccgaagg
     1741 cttccccttc gttcgacctt aagcgttggt gggagaagaa cgaactggtc acatctgacc
     1801 gtacctctaa ccgtcgtctg cagcagaccc aggctcaggt tgaagaagtt gttgacatca
     1861 tccgtgttaa cgttgacaaa gttctggaac gtgaccagaa actgtctgaa ctggacgacc
     1921 gtgctgacgc tctgcaggct ggtgcttctc agttcgaatc ttctgctgct aaactgaaac
     1981 gtaacacgat taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt
     2041 tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc
     2101 atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag
     2161 ctggtgaggt tgcggataac gctgccgcta agcctctcat cactacccta ctccctaaga
     2221 tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga
     2281 cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga
     2341 ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa
     2401 tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc
     2461 acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagaaag
     2521 catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt ggcgaggcgt
     2581 ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc gagatgctca
     2641 ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta ggtcaagact
     2701 ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc
     2761 tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg tggactggca
     2821 ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca
     2881 gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac aaagcgatta
     2941 acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc gccaacgtaa
     3001 tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt gaagaactcc
     3061 cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg
     3121 ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc cttgagttca
     3181 tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct tacaacatgg
     3241 actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca
     3301 aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac tactggctga
     3361 aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt
     3421 tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg gagaacactt
     3481 ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag tacgctgggg
     3541 tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac gggtcttgct
     3601 ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc gcggttaact
     3661 tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa gtcaacgaga
     3721 ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg accgatgaga
     3781 acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct ggtcaatggc
     3841 tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg gcttacgggt
     3901 ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca gctattgatt
     3961 ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga
     4021 tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt
     4081 ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca
     4141 agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga
     4201 agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta cagcctacca
     4261 ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta
     4321 actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga
     4381 agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt ccggctgacg
     4441 ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg
     4501 tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa
     4561 tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg
     4621 cgttcgcgta atggagattt tcaacatgac gccatgggca ttaaaaaatg ccctcttggg
     4681 ttatcaagag ggtcattata tttgctgagg agtgccgtta attaagttta ggtatattcc
     4741 gtgtggtact tcttaatatt atcatcaaca atattgatta catttttttg gctcatcaaa
     4801 tcattcattg gttcaaagga cagcaataca cttttcgcac cacacttttc aattgccaac
     4861 ttagccgcag ttatacactc cgtataattt ccgacagcgt tttctgcaat tatttcttca
     4921 agtttatttt cgaaattttc attagggtgc atttcaagaa cataatcact aataaatgca
     4981 cgcgtctctt gtttagcttt attactatct tcgttatagt taactaatat cattaactga
     5041 tggtctatct ctgataggtc aacgtcatat ttatccgcaa cggctttata tctttcagca
     5101 tattcatatc taacatcatt agaatcatcc cacttaaaga tgagaggaat accttttttg
     5161 gccgcccact caacaatatg atgactggtt gctgttacat atttccgagg tccgcctggc
     5221 gtataagcat ggggatttac agatatttta gggaagctat aaaaatcgtt atctggatta
     5281 caatagcctg ttgttaaagc atcgttaatg atttcataac actcttcaaa tagttgctgt
     5341 tgatattcaa ccgggcgatt aaaaaaatgc atttcatctt ttttttcgca atcactaaac
     5401 cctaaaataa atctcccttc acttaactga tccaataagc aagcttcctc cgctatgcgg
     5461 acaggatgat gagttgtaat gatgtgattt aatgaaccaa ttttaatttt ctctgttaaa
     5521 ccgagcagaa aaccagaaac agtcagagga gcgccgacaa caccattatc tgaaaaatga
     5581 ttttcataca ctaaaatctg ttcaaaattc aacttatcaa catactccgt tatttcctgc
     5641 atgcgaacta tactctgttc ttgaacagtt gttgaattga tgaagttaag gaagaacaat
     5701 ccaaatttgc taccgccacc gccgctaccg ccaccgccgc taccgccacc gccgctaccg
     5761 ccaccgccat aatataatag cgaacgttgt ttttctttaa gaaatggcat gacatcagac
     5821 tggaagagct tcatggaagc aataatttcg tctactgttc cattagcttc aaatccacaa
     5881 caaatatttg atattcctgt agcatcaatg tctttttgaa ttatgtcaat acattcctgc
     5941 ggcgttccca cgggattgat ttcgtaactg taatcaatac ggcgattagt atctttatgt
     6001 ccttttaata caaagtcacg ccactgccct ttattgaaat cataacctct tgtttggtct
     6061 gaatcatcaa aaatagtcgt agcattcaca taagaatcat accaatgccc cagaaatttc
     6121 cggcaaatct ctttcgcttt aattgagtca tgatctacag atgttatata tgataagcaa
     6181 tggtcgatat tatgaatatc gtgcccatat tcttgagcca cttcattata aagctcaagt
     6241 tgtgctttct tttcgttagt atttataatc caacttaata tcatcggtag gccaaattga
     6301 gcagcccact cagtcgtcga agctgattca gccaccacat aaaccggtgc gccacctctg
     6361 ctatacgccg cggggtttac ttttacctta tggaacttga tatgttcatt atcagcttcc
     6421 atatatccct ctgtcatgcc attctttatc agcccgtacc agcattccgc taaggcgcga
     6481 ctgttattca tatctgtgcc gaatacgcga aagtccttgt tgtaaagccc tcggcaaata
     6541 ccaaaccgaa atcgtccttt tgacatttga tccaataaat tcacatcttc aagttggcgt
     6601 actggatggg ctgtgggaag aacaatagcg gcagttccta cattcaattt tttagtcgcg
     6661 ccaagtaaat atgcagcagc gacataaggg ttaccaagca aaccaaactc cgtgaaatga
     6721 tgctccagta accatacggt atcaaaacca cactcctcag agatgcgacc taatttaacc
     6781 aaacgtttca ttacctctgt ttgggaaaat tggggaggtt ggtatgtaag caaaaagttt
     6841 ccaaatttca ttaagactcc ttattacgca gtatgttagc aaacgtagaa aatacataca
     6901 taaaggtggc aacatataaa agaaacgcaa agacaccgcg gaacaggttg atcttatcgc
     6961 agtcgatact gaactcgtaa ggtttaccag cgccaaagac aaaagggcga cattcaaccg
     7021 attgagggag ggaaggtaaa tattgacgga aattattcat taaaggtgaa ttatcaccgt
     7081 caccgacttg agccatttgg gaattagagc cagcaaaatc accagtagca ccattaccat
     7141 tagcaaggcc ggaaacgtca ccaatgaaac catcgatagc agcaccgtaa tcagtagcga
     7201 cagaatcaag tttgccttta gcgtcagact gtagcgcgtt ttcatcggca ttttcggtca
     7261 tagccccctt attagcgttt gccatctttt cataatcaaa atcaccggaa ccagagccac
     7321 caccggaacc gcctccctca gagccgccac cctcagaacc gccaccctca gagccaccac
     7381 cctcagagcc gccaccagaa ccaccaccag agccgccgcc agcattgaca ggaggttgag
     7441 gcaggtcaga cgattggcct tgatattcac aaacgaatgg atcctcatta aagccagaat
     7501 ggaaagcgca gtctctgaat ttaccgttcc agtaagcgtc atacatggct tttgatgata
     7561 caggagtgta ctggtaataa gttttaacgg ggtcagtgcc ttgagtaaca gtgcccgtat
     7621 aaacagttaa tgccccctgc ctatttcgga acctattatt ctgaaacatg aaagtattaa
     7681 gaggctgaga ctcctcaaga gaaggattag gattagcggg gttttgctca gtaccaggcg
     7741 gataagtgcc gtcgagaggg ttgatataag tatagcccgg aataggtgta tcaccgtact
     7801 caggaggttt agtaccgcca ccctcagaac cgccaccctc agaaccgcca ccctcagagc
     7861 caccaccctc attttcaggg atagcaagcc caataggaac ccatgtaccg taacactgag
     7921 tttcgtcacc agtacaaact acaacgcctg tagcattcca cagacagccc tcatagttag
     7981 cgtaacgatc taaagttttg tcgtctttcc agacgttagt aaatgaattt tctgtatggg
     8041 gttttgctaa acaactttca acagtttcag cggagtgaga atagaaagga acaactaaag
     8101 gaattgcgaa taataatttt ttcatttttt tttcctttac tgcacctgca ggtctttctt
     8161 atgagttacc tcctgaactt ggtaaacaga acaacaaagc tgcctgatag tggcctttat
     8221 ttttggcata aataacagaa taaacactgc actgtgtatt cattccaacg agtgaataca
     8281 cggagcaatg tcgctccttc tttctcccta tagtgagtcg tattagtagg gtccctaagt
     8341 ctcctcagca aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgaccgga
     8401 tgtcctcttg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca
     8461 ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga
     8521 gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa
     8581 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat
     8641 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc
     8701 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa
     8761 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc
     8821 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact
     8881 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct
     8941 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg
     9001 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac
     9061 agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc catctcaatt
     9121 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca
     9181 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt
     9241 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg
     9301 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg
     9361 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt
     9421 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat
     9481 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa
     9541 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt
     9601 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg
     9661 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc
     9721 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg
     9781 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa
     9841 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa
     9901 caaccattac atcagattcc tacctacgta acggactaag aaaaacacta cacgatgctt
     9961 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta
    10021 agcatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga
    10081 acatactggc taaatacgga aggatctga
//
