LOCUS       sequence-33080-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP522
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(17..50)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctagaggatc cccgggataa cttcgtatag catacattat acgaagttat taaccattat
       61 ttttttcctc aacataacga gaacacacag gggcgctatc gcacagaatc aaattcgatg
      121 actggaaatt ttttgttaat ttcagaggtc gcctgacgca tatacctttt tcaactgaaa
      181 aattgggaga aaaaggaaag gtgagagcgc cggaaccggc ttttcatata gaatagagaa
      241 gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta tttaaggacc
      301 tattgttttt ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta
      361 catttcagca atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga
      421 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
      481 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
      541 ttggtggtgc tgctatcgaa gctacaggtg tcccacttcc agatgaggcg ctggaagcct
      601 ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtacaggta
      661 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
      721 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
      781 ttgctaaagg tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta
      841 agagaaagga agatgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
      901 aagtgcaaag aatcacaaga atggcgcttc tggctcac
//
