LOCUS       sequence-330804-       10164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: APT3-VAMP7
FEATURES             Location/Qualifiers
     source          1..10164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1355..1807
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4784..5764)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5828..6907)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6907..8181)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     promoter        complement(8362..8380)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      8944..9166
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9214..10164
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaaaatggct cgtgtacagt ttaaacaacg
     1381 tgaatctact gacgcaatct ttgttcactg ctcggctacc aagccaagtc agaatgttgg
     1441 tgtccgtgag attcgccagt ggcacaaaga gcagggttgg ctcgatgtgg gataccactt
     1501 tatcatcaag cgagacggta ctgtggaggc aggacgagat gagatggctg taggctctca
     1561 cgctaagggt tacaaccaca actctatcgg cgtctgcctt gttggtggta tcgacgataa
     1621 aggtaagttc gacgctaact ttacgccagc ccaaatgcaa tcccttcgct cactgcttgt
     1681 cacactgctg gctaagtacg aaggcgctgt gcttcgcgcc catcatgagg tggcgccgaa
     1741 ggcttcccct tcgttcgacc ttaagcgttg gtgggagaag aacgaactgg tcacatctga
     1801 ccgtggcggc tctggcggct ctggcggctc taaaggtctg gacaaagtta tggaaaccca
     1861 ggctcaggtt gacgaactga aaggtatcat ggttcgtaac atcgacctgg ttgctcagcg
     1921 tggtgaacgt ctggaactgc tgatcgacaa aaccgaaaac ctggttgact cttctgttac
     1981 cttcaaaacc acctctcgta acctggctcg tggcggctct ggcggctctg gcggctctaa
     2041 cacgattaac atcgctaaga acgacttctc tgacatcgaa ctggctgcta tcccgttcaa
     2101 cactctggct gaccattacg gtgagcgttt agctcgcgaa cagttggccc ttgagcatga
     2161 gtcttacgag atgggtgaag cacgcttccg caagatgttt gagcgtcaac ttaaagctgg
     2221 tgaggttgcg gataacgctg ccgccaagcc tctcatcact accctactcc ctaagatgat
     2281 tgcacgcatc aacgactggt ttgaggaagt gaaagctaag ctcggcaggc gcccgacagc
     2341 cttccagttc ctgcaagaaa tcaagccgga agccgtagcg tacatcacca ttaagaccac
     2401 tctggcttgc ctaaccagtg ctgacaatac aaccgttcag gctgtagcaa gcgcaatcgg
     2461 tcgggccatt gaggacgagg ctcgcttcgg tcgtatccgt gaccttgaag ctaagcactt
     2521 caagaaaaac gttgaggaac aactcaacaa gcgcgtaggg cacgtctaca agaaagcatt
     2581 tatgcaagtt gtcgaggctg acatgctctc taagggtcta ctcggtggcg aggcgtggtc
     2641 ttcgtggtat aagaaagact ctattcatgt aggagtacgc tgcatcgaga tgctcattaa
     2701 gtcaactgga atggttagct tacaccgcca aaatgctggc gtagtaggtc aagactctga
     2761 gactatcgaa ctcgcacctg aatacgctga ggctatcgca acccgtgcag gtgcgctggc
     2821 tggcatctct ccgatgttcc aaccttgcgt agttcctcct aagccgtgga ctggcattac
     2881 tggtggtggc tattgggcta acggtcgtcg tcctctggcg ctggtgcgta ctcacagtaa
     2941 gaaagcactg atgcgctacg aagacgttta catgcctgag gtgtacaaag cgattaacat
     3001 tgcgcaaaac accgcatgga aaatcaacaa gaaagtccta gcggtcgcca acgtaatcac
     3061 caagtggaag cattgtccgg tcgaggacat ccctgcgatt gagcgtgaag aactcccgat
     3121 gaaaccggaa gacatcgaca tgaatcctga ggctctcacc gcgtggaaac gtgctgccgc
     3181 tgctgtgtac cgcaaggaca aggctcgcaa gtctcgccgt atcagacttg agttcatgct
     3241 tgagcaagcc aataagtttg ctaaccataa ggccatctgg ttcccttaca acatggactg
     3301 gcgcggtcgt gtttacgctg tgtcaatgtt caacccgcaa ggtaacgata tgaccaaagg
     3361 actgcttacg ctggcgaaag gtaaaccaat cggtaaggaa ggttactact ggctgaaaat
     3421 ccacggtgca aactgtgcgg gtgtcgataa ggttccgttc cctgagcgca tcaagttcat
     3481 tgaggaaaac cacgagaaca tcatggcttg cgctaagtct ccactggaga acacttggtg
     3541 ggctgagcaa gattctccgt tctgcttcct tgcgttctgc tttgagtacg ctggggtaca
     3601 gcaccacggc ctgagctata actgctccct tccgctggcg tttgacgggt cttgctctgt
     3661 catccagcac ttctccgcga tgctccgaga tgaggtaggt ggtcgcgcgg ttaacttgct
     3721 tcctagtgaa accgttcagg acatctacgg gattgttgct aagaaagtca acgagattct
     3781 acaagcagac gcaatcaatg ggaccgataa cgaagtagtt accgtgaccg atgagaacac
     3841 tggtgaaatc tctgagaaag tcaagctggg cactaaggca ctggctggtc aatggctggc
     3901 ttacggtgtt actcgcagtg tgactaagcg ttcagtcatg acgctggctt acgggtccaa
     3961 agagttcggc ttccgtcaac aagtgctgga cgataccatt cagccagcta ttgattccgg
     4021 caagggtctg atgttcaccc agccgaatca ggctgctaga tacatggcta agctgatttg
     4081 ggaatctgtg agcgtgacgg tggtagctgc ggttgaagca atgaactggc ttaagtctgc
     4141 tgctaagctg ctggctgctg aggtcaaaga taagaagact ggagagattc ttcgcaagcg
     4201 ttgcgctgtg cattggataa ctcctgatgg tttccctgtg tggcaggaat acaagaagcc
     4261 tattcagacg cgcttggacc tgatgttcct cggtcagttc cgcttacagc ttaccattaa
     4321 caccaacaaa gatagcgaga ttgatgcaca caaacaggag gctggtatcg ctcctaactt
     4381 tgtacacagc caagacggta gccaccttcg taagactgta gtgtgggcac acgagaagta
     4441 cggaatcgaa tcttttgcac tgattcacga ctccttcggt accattccgg ctgacgctgc
     4501 gaacctgttc aaagcagtgc gcgaaactat ggttgacaca tatgagtctt gtgatgtact
     4561 ggctgatttc tacgaccagt tcgctgacca gttgcacgag tctcaattgg acaaaatgcc
     4621 agcacttccg gctaaaggta acttgaacct ccgtgacatc ttagagtcgg acttcgcgtt
     4681 cgcgtaatgg agattttcaa catgacgcca tgggcattaa aaaatgccct cttgggttat
     4741 caagagggtc attatatttg ctgaggagtg ccgttaatta agtttaggta tattccgtgt
     4801 ggtacttctt aatattatca tcaacaatat tgattacatt tttttggctc atcaaatcat
     4861 tcattggttc aaaggacagc aatacacttt tcgcaccaca cttttcaatt gccaacttag
     4921 ccgcagttat acactccgta taatttccga cagcgttttc tgcaattatt tcttcaagtt
     4981 tattttcgaa attttcatta gggtgcattt caagaacata atcactaata aatgcacgcg
     5041 tctcttgttt agctttatta ctatcttcgt tatagttaac taatatcatt aactgatggt
     5101 ctatctctga taggtcaacg tcatatttat ccgcaacggc tttatatctt tcagcatatt
     5161 catatctaac atcattagaa tcatcccact taaagatgag aggaatacct tttttggccg
     5221 cccactcaac aatatgatga ctggttgctg ttacatattt ccgaggtccg cctggcgtat
     5281 aagcatgggg atttacagat attttaggga agctataaaa atcgttatct ggattacaat
     5341 agcctgttgt taaagcatcg ttaatgattt cataacactc ttcaaatagt tgctgttgat
     5401 attcaaccgg gcgattaaaa aaatgcattt catctttttt ttcgcaatca ctaaacccta
     5461 aaataaatct cccttcactt aactgatcca ataagcaagc ttcctccgct atgcggacag
     5521 gatgatgagt tgtaatgatg tgatttaatg aaccaatttt aattttctct gttaaaccga
     5581 gcagaaaacc agaaacagtc agaggagcgc cgacaacacc attatctgaa aaatgatttt
     5641 catacactaa aatctgttca aaattcaact tatcaacata ctccgttatt tcctgcatgc
     5701 gaactatact ctgttcttga acagttgttg aattgatgaa gttaaggaag aacaatccaa
     5761 atttgctacc gccaccgccg ctaccgccac cgccgctacc gccaccgccg ctaccgccac
     5821 cgccataata taatagcgaa cgttgttttt ctttaagaaa tggcatgaca tcagactgga
     5881 agagcttcat ggaagcaata atttcgtcta ctgttccatt agcttcaaat ccacaacaaa
     5941 tatttgatat tcctgtagca tcaatgtctt tttgaattat gtcaatacat tcctgcggcg
     6001 ttcccacggg attgatttcg taactgtaat caatacggcg attagtatct ttatgtcctt
     6061 ttaatacaaa gtcacgccac tgccctttat tgaaatcata acctcttgtt tggtctgaat
     6121 catcaaaaat agtcgtagca ttcacataag aatcatacca atgccccaga aatttccggc
     6181 aaatctcttt cgctttaatt gagtcatgat ctacagatgt tatatatgat aagcaatggt
     6241 cgatattatg aatatcgtgc ccatattctt gagccacttc attataaagc tcaagttgtg
     6301 ctttcttttc gttagtattt ataatccaac ttaatatcat cggtaggcca aattgagcag
     6361 cccactcagt cgtcgaagct gattcagcca ccacataaac cggtgcgcca cctctgctat
     6421 acgccgcggg gtttactttt accttatgga acttgatatg ttcattatca gcttccatat
     6481 atccctctgt catgccattc tttatcagcc cgtaccagca ttccgctaag gcgcgactgt
     6541 tattcatatc tgtgccgaat acgcgaaagt ccttgttgta aagccctcgg caaataccaa
     6601 accgaaatcg tccttttgac atttgatcca ataaattcac atcttcaagt tggcgtactg
     6661 gatgggctgt gggaagaaca atagcggcag ttcctacatt caatttttta gtcgcgccaa
     6721 gtaaatatgc agcagcgaca taagggttac caagcaaacc aaactccgtg aaatgatgct
     6781 ccagtaacca tacggtatca aaaccacact cctcagagat gcgacctaat ttaaccaaac
     6841 gtttcattac ctctgtttgg gaaaattggg gaggttggta tgtaagcaaa aagtttccaa
     6901 atttcattaa gactccttat tacgcagtat gttagcaaac gtagaaaata catacataaa
     6961 ggtggcaaca tataaaagaa acgcaaagac accgcggaac aggttgatct tatcgcagtc
     7021 gatactgaac tcgtaaggtt taccagcgcc aaagacaaaa gggcgacatt caaccgattg
     7081 agggagggaa ggtaaatatt gacggaaatt attcattaaa ggtgaattat caccgtcacc
     7141 gacttgagcc atttgggaat tagagccagc aaaatcacca gtagcaccat taccattagc
     7201 aaggccggaa acgtcaccaa tgaaaccatc gatagcagca ccgtaatcag tagcgacaga
     7261 atcaagtttg cctttagcgt cagactgtag cgcgttttca tcggcatttt cggtcatagc
     7321 ccccttatta gcgtttgcca tcttttcata atcaaaatca ccggaaccag agccaccacc
     7381 ggaaccgcct ccctcagagc cgccaccctc agaaccgcca ccctcagagc caccaccctc
     7441 agagccgcca ccagaaccac caccagagcc gccgccagca ttgacaggag gttgaggcag
     7501 gtcagacgat tggccttgat attcacaaac gaatggatcc tcattaaagc cagaatggaa
     7561 agcgcagtct ctgaatttac cgttccagta agcgtcatac atggcttttg atgatacagg
     7621 agtgtactgg taataagttt taacggggtc agtgccttga gtaacagtgc ccgtataaac
     7681 agttaatgcc ccctgcctat ttcggaacct attattctga aacatgaaag tattaagagg
     7741 ctgagactcc tcaagagaag gattaggatt agcggggttt tgctcagtac caggcggata
     7801 agtgccgtcg agagggttga tataagtata gcccggaata ggtgtatcac cgtactcagg
     7861 aggtttagta ccgccaccct cagaaccgcc accctcagaa ccgccaccct cagagccacc
     7921 accctcattt tcagggatag caagcccaat aggaacccat gtaccgtaac actgagtttc
     7981 gtcaccagta caaactacaa cgcctgtagc attccacaga cagccctcat agttagcgta
     8041 acgatctaaa gttttgtcgt ctttccagac gttagtaaat gaattttctg tatggggttt
     8101 tgctaaacaa ctttcaacag tttcagcgga gtgagaatag aaaggaacaa ctaaaggaat
     8161 tgcgaataat aattttttca ttttttcctt tttactgcac ctgcaggtct ttcttatgag
     8221 ttacctcctg aacttggtaa acagaacaac aaagctgcct gatagtggcc tttatttttg
     8281 gcataaataa cagaataaac actgcactgt gtattcattc caacgagtga atacacggag
     8341 caatgtcgct ccttctttct ccctttagtg agggttaatt gtagggtccc taagtctcct
     8401 cagcaaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga ccggatgtcc
     8461 tcttgacagt aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc
     8521 cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca
     8581 ggaactgctg aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc
     8641 ctgagaagcc cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata
     8701 aaaggcgcct gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact
     8761 gaatgtcacg aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc
     8821 agcgatttgc ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt
     8881 tgtagaggag caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt
     8941 agtgagttat acacagggct gggatctatt ctttttatct ttttttattc tttctttatt
     9001 ctataaatta taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt
     9061 acagagggtc tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata
     9121 cccacaactc aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat
     9181 agtgattaaa atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca
     9241 aatgaactag cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc
     9301 tgtgtggcac tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg
     9361 ttcacttata accaatacgc tcagatgatg aacatcagta gggaaaatgc ttatggtgta
     9421 ttagctaaag caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt
     9481 aaaggctttg agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa
     9541 ttagttttta gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat
     9601 aatctggaac atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta
     9661 ttaaaagaac taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa
     9721 tttaagttca tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg
     9781 gttttgaaac caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat
     9841 aagcgaggcc gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat
     9901 ctcgtaaccg aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc
     9961 attacatcag attcctacct acgtaacgga ctaagaaaaa cactacacga tgctttaact
    10021 gcaaaaattc agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagcat
    10081 gatctcaatg gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata
    10141 ctggctaaat acggaaggat ctga
//
