LOCUS       sequence-330805-       10163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10163)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: AP-VAMP7(low)
FEATURES             Location/Qualifiers
     source          1..10163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1353..1805
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4782..5762)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5826..6905)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6905..8179)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     primer_bind     complement(8360..8379)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8361..8379)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8943..9165
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9213..10163
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaatggctcg tgtacagttt aaacaacgtg
     1381 aatctactga cgcaatcttt gttcactgct cggctaccaa gccaagtcag aatgttggtg
     1441 tccgtgagat tcgccagtgg cacaaagagc agggttggct cgatgtggga taccacttta
     1501 tcatcaagcg agacggtact gtggaggcag gacgagatga gatggctgta ggctctcacg
     1561 ctaagggtta caaccacaac tctatcggcg tctgccttgt tggtggtatc gacgataaag
     1621 gtaagttcga cgctaacttt acgccagccc aaatgcaatc ccttcgctca ctgcttgtca
     1681 cactgctggc taagtacgaa ggcgctgtgc ttcgcgccca tcatgaggtg gcgccgaagg
     1741 cttccccttc gttcgacctt aagcgttggt gggagaagaa cgaactggtc acatctgacc
     1801 gtggcggctc tggcggctct ggcggctcta aaggtctgga caaagttatg gaaacccagg
     1861 ctcaggttga cgaactgaaa ggtatcatgg ttcgtaacat cgacctggtt gctcagcgtg
     1921 gtgaacgtct ggaactgctg atcgacaaaa ccgaaaacct ggttgactct tctgttacct
     1981 tcaaaaccac ctctcgtaac ctggctcgtg gcggctctgg cggctctggc ggctctaaca
     2041 cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca
     2101 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt
     2161 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg
     2221 aggttgcgga taacgctgcc gctaagcctc tcatcactac cctactccct aagatgattg
     2281 cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct
     2341 tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc
     2401 tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc
     2461 gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca
     2521 agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta
     2581 tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt
     2641 cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt
     2701 caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga
     2761 ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg
     2821 gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg
     2881 gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga
     2941 aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg
     3001 cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca
     3061 agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga
     3121 aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg
     3181 ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg
     3241 agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc
     3301 gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac
     3361 tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc
     3421 acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc aagttcattg
     3481 aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg
     3541 ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct ggggtacagc
     3601 accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca
     3661 tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc
     3721 ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac
     3781 aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg
     3841 gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa tggctggctt
     3901 acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag
     3961 agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca
     4021 agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg
     4081 aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg
     4141 ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt
     4201 gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta
     4261 ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca
     4321 ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg
     4381 tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg
     4441 gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga
     4501 acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg
     4561 ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag
     4621 cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg
     4681 cgtaatggag attttcaaca tgacgccatg ggcattaaaa aatgccctct tgggttatca
     4741 agagggtcat tatatttgct gaggagtgcc gttaattaag tttaggtata ttccgtgtgg
     4801 tacttcttaa tattatcatc aacaatattg attacatttt tttggctcat caaatcattc
     4861 attggttcaa aggacagcaa tacacttttc gcaccacact tttcaattgc caacttagcc
     4921 gcagttatac actccgtata atttccgaca gcgttttctg caattatttc ttcaagttta
     4981 ttttcgaaat tttcattagg gtgcatttca agaacataat cactaataaa tgcacgcgtc
     5041 tcttgtttag ctttattact atcttcgtta tagttaacta atatcattaa ctgatggtct
     5101 atctctgata ggtcaacgtc atatttatcc gcaacggctt tatatctttc agcatattca
     5161 tatctaacat cattagaatc atcccactta aagatgagag gaataccttt tttggccgcc
     5221 cactcaacaa tatgatgact ggttgctgtt acatatttcc gaggtccgcc tggcgtataa
     5281 gcatggggat ttacagatat tttagggaag ctataaaaat cgttatctgg attacaatag
     5341 cctgttgtta aagcatcgtt aatgatttca taacactctt caaatagttg ctgttgatat
     5401 tcaaccgggc gattaaaaaa atgcatttca tctttttttt cgcaatcact aaaccctaaa
     5461 ataaatctcc cttcacttaa ctgatccaat aagcaagctt cctccgctat gcggacagga
     5521 tgatgagttg taatgatgtg atttaatgaa ccaattttaa ttttctctgt taaaccgagc
     5581 agaaaaccag aaacagtcag aggagcgccg acaacaccat tatctgaaaa atgattttca
     5641 tacactaaaa tctgttcaaa attcaactta tcaacatact ccgttatttc ctgcatgcga
     5701 actatactct gttcttgaac agttgttgaa ttgatgaagt taaggaagaa caatccaaat
     5761 ttgctaccgc caccgccgct accgccaccg ccgctaccgc caccgccgct accgccaccg
     5821 ccataatata atagcgaacg ttgtttttct ttaagaaatg gcatgacatc agactggaag
     5881 agcttcatgg aagcaataat ttcgtctact gttccattag cttcaaatcc acaacaaata
     5941 tttgatattc ctgtagcatc aatgtctttt tgaattatgt caatacattc ctgcggcgtt
     6001 cccacgggat tgatttcgta actgtaatca atacggcgat tagtatcttt atgtcctttt
     6061 aatacaaagt cacgccactg ccctttattg aaatcataac ctcttgtttg gtctgaatca
     6121 tcaaaaatag tcgtagcatt cacataagaa tcataccaat gccccagaaa tttccggcaa
     6181 atctctttcg ctttaattga gtcatgatct acagatgtta tatatgataa gcaatggtcg
     6241 atattatgaa tatcgtgccc atattcttga gccacttcat tataaagctc aagttgtgct
     6301 ttcttttcgt tagtatttat aatccaactt aatatcatcg gtaggccaaa ttgagcagcc
     6361 cactcagtcg tcgaagctga ttcagccacc acataaaccg gtgcgccacc tctgctatac
     6421 gccgcggggt ttacttttac cttatggaac ttgatatgtt cattatcagc ttccatatat
     6481 ccctctgtca tgccattctt tatcagcccg taccagcatt ccgctaaggc gcgactgtta
     6541 ttcatatctg tgccgaatac gcgaaagtcc ttgttgtaaa gccctcggca aataccaaac
     6601 cgaaatcgtc cttttgacat ttgatccaat aaattcacat cttcaagttg gcgtactgga
     6661 tgggctgtgg gaagaacaat agcggcagtt cctacattca attttttagt cgcgccaagt
     6721 aaatatgcag cagcgacata agggttacca agcaaaccaa actccgtgaa atgatgctcc
     6781 agtaaccata cggtatcaaa accacactcc tcagagatgc gacctaattt aaccaaacgt
     6841 ttcattacct ctgtttggga aaattgggga ggttggtatg taagcaaaaa gtttccaaat
     6901 ttcattaaga ctccttatta cgcagtatgt tagcaaacgt agaaaataca tacataaagg
     6961 tggcaacata taaaagaaac gcaaagacac cgcggaacag gttgatctta tcgcagtcga
     7021 tactgaactc gtaaggttta ccagcgccaa agacaaaagg gcgacattca accgattgag
     7081 ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca ccgtcaccga
     7141 cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta ccattagcaa
     7201 ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta gcgacagaat
     7261 caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg gtcatagccc
     7321 ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag ccaccaccgg
     7381 aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca ccaccctcag
     7441 agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggt tgaggcaggt
     7501 cagacgattg gccttgatat tcacaaacga atggatcctc attaaagcca gaatggaaag
     7561 cgcagtctct gaatttaccg ttccagtaag cgtcatacat ggcttttgat gatacaggag
     7621 tgtactggta ataagtttta acggggtcag tgccttgagt aacagtgccc gtataaacag
     7681 ttaatgcccc ctgcctattt cggaacctat tattctgaaa catgaaagta ttaagaggct
     7741 gagactcctc aagagaagga ttaggattag cggggttttg ctcagtacca ggcggataag
     7801 tgccgtcgag agggttgata taagtatagc ccggaatagg tgtatcaccg tactcaggag
     7861 gtttagtacc gccaccctca gaaccgccac cctcagaacc gccaccctca gagccaccac
     7921 cctcattttc agggatagca agcccaatag gaacccatgt accgtaacac tgagtttcgt
     7981 caccagtaca aactacaacg cctgtagcat tccacagaca gccctcatag ttagcgtaac
     8041 gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta tggggttttg
     8101 ctaaacaact ttcaacagtt tcagcggagt gagaatagaa aggaacaact aaaggaattg
     8161 cgaataataa ttttttcatt tttttttcct ttactgcacc tgcaggtctt tcttatgagt
     8221 tacctcctga acttggtaaa cagaacaaca aagctgcctg atagtggcct ttatttttgg
     8281 cataaataac agaataaaca ctgcactgtg tattcattcc aacgagtgaa tacacggagc
     8341 aatgtcgctc cttctttctc cctatagtga gtcgtattag tagggtccct aagtctcctc
     8401 agcaaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgac cggatgtcct
     8461 cttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc
     8521 agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag
     8581 gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc
     8641 tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa
     8701 aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg
     8761 aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca
     8821 gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt
     8881 gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta
     8941 gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc
     9001 tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta
     9061 cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac
     9121 ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata
     9181 gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa
     9241 atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct
     9301 gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt
     9361 tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat
     9421 tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta
     9481 aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat
     9541 tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata
     9601 atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat
     9661 taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat
     9721 ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg
     9781 ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata
     9841 agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc
     9901 tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca
     9961 ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg
    10021 caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg
    10081 atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac
    10141 tggctaaata cggaaggatc tga
//
