LOCUS       sequence-330806-       10053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: AP-SNAP25
FEATURES             Location/Qualifiers
     source          1..10053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        107..211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(212..280,281..1072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(430..449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1123..1169
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1354..1806
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4672..5652)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5716..6795)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6795..8069)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     primer_bind     complement(8250..8269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8251..8269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8833..9055
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9103..10053
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 acggcctatt tggcctaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      121 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      181 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      241 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      301 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      361 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      421 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      481 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      541 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      601 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      661 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      721 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
      781 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      841 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
      901 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
      961 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1021 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aacgtaatgc
     1081 tctgccagtg ttacaaccaa ttaaccaatt ctgatttccg gtcaaataaa acgaaaggct
     1141 cagtcgaaag actgggcctt tcgttttatt ctagagcaca gctaacacca cgtcgtccct
     1201 atctgctgcc ctaggtctat gagtggttgc tggataactt tacgggcatg cataaggctc
     1261 gtaatatata ttcagggaga ccacaacggt ttccctctac aaataatttt gtttaacttt
     1321 tactagagtt ccttcctcga aaggaaaaaa aaaatggctc gtgtacagtt taaacaacgt
     1381 gaatctactg acgcaatctt tgttcactgc tcggctacca agccaagtca gaatgttggt
     1441 gtccgtgaga ttcgccagtg gcacaaagag cagggttggc tcgatgtggg ataccacttt
     1501 atcatcaagc gagacggtac tgtggaggca ggacgagatg agatggctgt aggctctcac
     1561 gctaagggtt acaaccacaa ctctatcggc gtctgccttg ttggtggtat cgacgataaa
     1621 ggtaagttcg acgctaactt tacgccagcc caaatgcaat cccttcgctc actgcttgtc
     1681 acactgctgg ctaagtacga aggcgctgtg cttcgcgccc atcatgaggt ggcgccgaag
     1741 gcttcccctt cgttcgacct taagcgttgg tgggagaaga acgaactggt cacttctgac
     1801 cgtggtagcg gcggtggtgc gagtggtggt gcggacatgg gtaacgaaat cgacacccag
     1861 aaccgtcaga tcgaccgtat catggaaaaa gctgacggcg gtagcgcagg cagtggagcg
     1921 ggcggtaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc
     1981 ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt
     2041 gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt
     2101 aaagctggtg aggttgcgga taacgctgcc gctaagcctc tcatcactac cctactccct
     2161 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc
     2221 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt
     2281 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc
     2341 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct
     2401 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag
     2461 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag
     2521 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg
     2581 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa
     2641 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt
     2701 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact
     2761 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact
     2821 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg
     2881 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac
     2941 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa
     3001 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt
     3061 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag
     3121 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac
     3181 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg
     3241 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg
     3301 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc
     3361 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac
     3421 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct
     3481 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct
     3541 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt
     3601 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac
     3661 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat
     3721 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa
     3781 tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac gctggcttac
     3841 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt
     3901 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag
     3961 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt
     4021 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt
     4081 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac
     4141 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct
     4201 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct
     4261 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac
     4321 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac cattccggct
     4381 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt
     4441 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac
     4501 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac
     4561 ttcgcgttcg cgtaatggag attttcaaca tgacgccatg ggcattaaaa aatgccctct
     4621 tgggttatca agagggtcat tatatttgct gaggagtgcc gttaattaag tttaggtata
     4681 ttccgtgtgg tacttcttaa tattatcatc aacaatattg attacatttt tttggctcat
     4741 caaatcattc attggttcaa aggacagcaa tacacttttc gcaccacact tttcaattgc
     4801 caacttagcc gcagttatac actccgtata atttccgaca gcgttttctg caattatttc
     4861 ttcaagttta ttttcgaaat tttcattagg gtgcatttca agaacataat cactaataaa
     4921 tgcacgcgtc tcttgtttag ctttattact atcttcgtta tagttaacta atatcattaa
     4981 ctgatggtct atctctgata ggtcaacgtc atatttatcc gcaacggctt tatatctttc
     5041 agcatattca tatctaacat cattagaatc atcccactta aagatgagag gaataccttt
     5101 tttggccgcc cactcaacaa tatgatgact ggttgctgtt acatatttcc gaggtccgcc
     5161 tggcgtataa gcatggggat ttacagatat tttagggaag ctataaaaat cgttatctgg
     5221 attacaatag cctgttgtta aagcatcgtt aatgatttca taacactctt caaatagttg
     5281 ctgttgatat tcaaccgggc gattaaaaaa atgcatttca tctttttttt cgcaatcact
     5341 aaaccctaaa ataaatctcc cttcacttaa ctgatccaat aagcaagctt cctccgctat
     5401 gcggacagga tgatgagttg taatgatgtg atttaatgaa ccaattttaa ttttctctgt
     5461 taaaccgagc agaaaaccag aaacagtcag aggagcgccg acaacaccat tatctgaaaa
     5521 atgattttca tacactaaaa tctgttcaaa attcaactta tcaacatact ccgttatttc
     5581 ctgcatgcga actatactct gttcttgaac agttgttgaa ttgatgaagt taaggaagaa
     5641 caatccaaat ttgctaccgc caccgccgct accgccaccg ccgctaccgc caccgccgct
     5701 accgccaccg ccataatata atagcgaacg ttgtttttct ttaagaaatg gcatgacatc
     5761 agactggaag agcttcatgg aagcaataat ttcgtctact gttccattag cttcaaatcc
     5821 acaacaaata tttgatattc ctgtagcatc aatgtctttt tgaattatgt caatacattc
     5881 ctgcggcgtt cccacgggat tgatttcgta actgtaatca atacggcgat tagtatcttt
     5941 atgtcctttt aatacaaagt cacgccactg ccctttattg aaatcataac ctcttgtttg
     6001 gtctgaatca tcaaaaatag tcgtagcatt cacataagaa tcataccaat gccccagaaa
     6061 tttccggcaa atctctttcg ctttaattga gtcatgatct acagatgtta tatatgataa
     6121 gcaatggtcg atattatgaa tatcgtgccc atattcttga gccacttcat tataaagctc
     6181 aagttgtgct ttcttttcgt tagtatttat aatccaactt aatatcatcg gtaggccaaa
     6241 ttgagcagcc cactcagtcg tcgaagctga ttcagccacc acataaaccg gtgcgccacc
     6301 tctgctatac gccgcggggt ttacttttac cttatggaac ttgatatgtt cattatcagc
     6361 ttccatatat ccctctgtca tgccattctt tatcagcccg taccagcatt ccgctaaggc
     6421 gcgactgtta ttcatatctg tgccgaatac gcgaaagtcc ttgttgtaaa gccctcggca
     6481 aataccaaac cgaaatcgtc cttttgacat ttgatccaat aaattcacat cttcaagttg
     6541 gcgtactgga tgggctgtgg gaagaacaat agcggcagtt cctacattca attttttagt
     6601 cgcgccaagt aaatatgcag cagcgacata agggttacca agcaaaccaa actccgtgaa
     6661 atgatgctcc agtaaccata cggtatcaaa accacactcc tcagagatgc gacctaattt
     6721 aaccaaacgt ttcattacct ctgtttggga aaattgggga ggttggtatg taagcaaaaa
     6781 gtttccaaat ttcattaaga ctccttatta cgcagtatgt tagcaaacgt agaaaataca
     6841 tacataaagg tggcaacata taaaagaaac gcaaagacac cgcggaacag gttgatctta
     6901 tcgcagtcga tactgaactc gtaaggttta ccagcgccaa agacaaaagg gcgacattca
     6961 accgattgag ggagggaagg taaatattga cggaaattat tcattaaagg tgaattatca
     7021 ccgtcaccga cttgagccat ttgggaatta gagccagcaa aatcaccagt agcaccatta
     7081 ccattagcaa ggccggaaac gtcaccaatg aaaccatcga tagcagcacc gtaatcagta
     7141 gcgacagaat caagtttgcc tttagcgtca gactgtagcg cgttttcatc ggcattttcg
     7201 gtcatagccc ccttattagc gtttgccatc ttttcataat caaaatcacc ggaaccagag
     7261 ccaccaccgg aaccgcctcc ctcagagccg ccaccctcag aaccgccacc ctcagagcca
     7321 ccaccctcag agccgccacc agaaccacca ccagagccgc cgccagcatt gacaggaggt
     7381 tgaggcaggt cagacgattg gccttgatat tcacaaacga atggatcctc attaaagcca
     7441 gaatggaaag cgcagtctct gaatttaccg ttccagtaag cgtcatacat ggcttttgat
     7501 gatacaggag tgtactggta ataagtttta acggggtcag tgccttgagt aacagtgccc
     7561 gtataaacag ttaatgcccc ctgcctattt cggaacctat tattctgaaa catgaaagta
     7621 ttaagaggct gagactcctc aagagaagga ttaggattag cggggttttg ctcagtacca
     7681 ggcggataag tgccgtcgag agggttgata taagtatagc ccggaatagg tgtatcaccg
     7741 tactcaggag gtttagtacc gccaccctca gaaccgccac cctcagaacc gccaccctca
     7801 gagccaccac cctcattttc agggatagca agcccaatag gaacccatgt accgtaacac
     7861 tgagtttcgt caccagtaca aactacaacg cctgtagcat tccacagaca gccctcatag
     7921 ttagcgtaac gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta
     7981 tggggttttg ctaaacaact ttcaacagtt tcagcggagt gagaatagaa aggaacaact
     8041 aaaggaattg cgaataataa ttttttcatt tttttttcct ttactgcacc tgcaggtctt
     8101 tcttatgagt tacctcctga acttggtaaa cagaacaaca aagctgcctg atagtggcct
     8161 ttatttttgg cataaataac agaataaaca ctgcactgtg tattcattcc aacgagtgaa
     8221 tacacggagc aatgtcgctc cttctttctc cctatagtga gtcgtattag tagggtccct
     8281 aagtctcctc agcaaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgac
     8341 cggatgtcct cttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg
     8401 tgcattagcc agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat
     8461 cagagggcag gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca
     8521 taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat
     8581 gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg
     8641 cagcgaactg aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa
     8701 ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa
     8761 ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact
     8821 gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt tttttattct
     8881 ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta
     8941 gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat
     9001 ttacagatac ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc
     9061 aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt
     9121 ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta
     9181 attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg
     9241 acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct
     9301 tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat
     9361 cctttggtta aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa
     9421 aaattagaat tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc
     9481 ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat
     9541 gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc
     9601 cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt
     9661 aaccaatggg ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg
     9721 gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga actagataga
     9781 caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata
     9841 ccaacaacca ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat
     9901 gctttaactg caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa
     9961 agtaagcatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta
    10021 gagaacatac tggctaaata cggaaggatc tga
//
