LOCUS       sequence-330811-        6682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter for mouse IRENE-div lncRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Hall JM, Kainth AS, Rowton MJ, Lu E, Haddad G, Keplinger AJ, 
            Scepanovic J , Perez-Cervantes C, Chan SSK, Piccirilli JA, Moskowitz
            IP, Ruthenburg AJ
  TITLE     The cardiac lncRNA Chantico directly regulates Cxcl1 chemokine 
            transcription
  JOURNAL   bioRxiv 2021.12.23.474040
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.12.23.474040"
COMMENT     Sequence Label: pIRENE-div-Firefly
FEATURES             Location/Qualifiers
     source          1..6682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2420..2451
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2478..2487
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2484..4136
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    4180..4301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4191..4210)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4245..4264
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4549..4566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4720..5308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4800..4819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5508..6299,6300..6368))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6473..6521
                     /note="synthetic polyadenylation signal"
     misc_feature    6535..6626
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6575..6594
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 agatatatag aattatatat atatgtatat tctacttgtt tctggggact ggactcgggg
       61 agcctcatat gctggtcaag tgctgagtca ctttattgag acatagtaag tctgaactac
      121 aattaacagg gtcataagca tactgtccca tgagtctgga catgtggaaa agctcgttga
      181 aagttcggtg aaatatagga accgctgaca ttttaaatga gcgcgtcccc gttagtcagt
      241 ccagggtctg tcacagactt gaagaagtac atcttccgca ggttttattt gtatgtttat
      301 ggaaaactca aatagcagaa tgtgcgggtt ttttttttca aattctgtgt gcttctttat
      361 atttttgcac ctaaaattta ttgttagttt cttgctgctc acactttcct cgtgcttttg
      421 gtggtgggga tatttccaaa acgcaaaatt tctctttggt cacttggggc tgtgattgat
      481 ttgatggatc cgatgggagc tgctttccga cctctgcagc ccttttcctt atctccgttt
      541 tctttcccct cagctccggg ctccgcagtg ccagtaacaa ggtcctccac ctgccctgac
      601 tgctgcaaac cgctacagcg ctacagcata aacggcggcg ctcaaaggtg ggggctctgc
      661 cggttccgaa ggatcgcgtc tcagaaggtg ataatttgaa aaatcaggtg acattgaagt
      721 gggtcgaggc ttcaaggtgc cttcagagct ctgctagcgg tggcttggag ctctgggaag
      781 cggctctgca gacgctttgg gcttgagagc tcagattgag cgacgcgcag ctgcgggcca
      841 ttcgctccta gcttcgcgga cgagccgggt ctccgagtgt gccgcgggac gtggtcagag
      901 tccaagagtc actgcccgaa cctggtggct gaaacccaac cggaagcctc ggagcctccg
      961 cctccctcca ggaccgctgg ggtctccggt gggcatgaat caggttgtaa gtggtcgctg
     1021 cacagtccca ggatcatcgc tgccccttct cccgctcctc cccgccgcac catctttttc
     1081 tttcagaccg tgctttacag cccttaatgt gcataaaata aactgtgtcc cacggaacat
     1141 gccgccttct ctcacgcggg ccgagaggat tgtgaaccgg taggcggcaa agtgtcacac
     1201 ggggaaagcc cagactacgg actgctggga atctgggtgt acccggagcc tccctgggtg
     1261 gcccgggact tcgaggggcc acctctgctc ccacgggatt cggccagggc acgaattggt
     1321 gaaaagcggg atggagacgc tagaggaaca caatgtcaca cgccaagctt tcaaggtgca
     1381 catgacggct gcagacactc aagcccttaa aaggagcatt taaacttggg ggggggggag
     1441 tacggatgga actcagaggc acaccaatta gtgtgaacac aacactcgcg gggactttgc
     1501 gggagcgttc acaattccgg agtgcacggg gaccgggagg tggctccgcc ccgcctcgct
     1561 ccagtcaaac ttcagtctct gccggtagaa aggcgtctct gcgctcccag ggatctactc
     1621 gcgcgtcgcc tgtacaaacc ctctgctgtg tggccttgta tctttaggta gggggacatc
     1681 ttttgtctct cggcaagaag ttttcctccg gggaagataa agtaatcgat agggcctttt
     1741 taaatagctc cgagtttcct gtcgggccag gagtatcagc gcgcacacca aatctgctct
     1801 ggtatgtcat cttatctccc ttccccgctg ttgtccccaa acgctgcctg tcaagagaga
     1861 cgccacccgc attaggaccc agactggggc ccttccgctc ggggtcacgc gctgccccct
     1921 cggctccgct gtccttcgcg ttcctggagg gtgggaagga gatgctgcag cccgcatgaa
     1981 gattctggga catggagcgg gtcactgggg cacactgtca ctcagaacgg gtgtgtgtgg
     2041 accgcttttc aaccacaaac acaaggaaca gccaaacagc gcattgactg cttgtctgtg
     2101 tgaagaaaaa ccaggctcat tcttcgaagg ggccattgga tctcagagcg ctccaggctg
     2161 gccccagctt tccctaactc caggatgact ttggaagggg agaggagagg gtctggcctc
     2221 agaatcctgc cgttttcctt tgcttcaact gctccacttc caacctgcag ggaaccggag
     2281 tggggttgct tagccaacac agaaaggcca aggtagtggt gacaagtgac caccgcttgg
     2341 gcagttcttt ctttctttct ctctttcttt ctttctttct tgatatcaag atctggcctc
     2401 ggcggccaag cttagacact agagggtata taatggaagc tcgacttcca gcttggcaat
     2461 ccggtactgt tggtaaagcc accatggaag atgccaaaaa cattaagaag ggcccagcgc
     2521 cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc atgaagcgct
     2581 acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg gacattacct
     2641 acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc tatgggctga
     2701 atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc atgcccgtgt
     2761 tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac aacgagcgcg
     2821 agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc aagaaagggc
     2881 tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc atcatcatgg
     2941 atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact tcccatttgc
     3001 cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac aaaaccatcg
     3061 ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc ctaccgcacc
     3121 gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac cagatcatcc
     3181 ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc
     3241 tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag gaggagctat
     3301 tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc acactattta
     3361 gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg cacgagatcg
     3421 ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa cgcttccacc
     3481 taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt ctgatcaccc
     3541 ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg
     3601 tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc
     3661 gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac gctctcatcg
     3721 acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac gagcacttct
     3781 tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta gccccagccg
     3841 aactggagag catcctgctg caacacccca acatcttcga cgccggggtc gccggcctgc
     3901 ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca
     3961 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc aagaagctgc
     4021 gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag ttggacgccc
     4081 gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc gtgtaataat
     4141 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     4201 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4321 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     4381 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
     4441 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     4501 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     4561 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4621 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4681 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4741 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4801 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4861 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4921 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4981 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5041 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5101 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5161 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5221 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5281 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5461 gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg
     5521 atcagtgagg caccgatctc agcgatctgc ctatttcgtt cgtccatagt ggcctgactc
     5581 cccgtcgtgt agatcactac gattcgtgag ggcttaccat caggccccag cgcagcaatg
     5641 atgccgcgag agccgcgttc accggccccc gatttgtcag caatgaacca gccagcaggg
     5701 agggccgagc gaagaagtgg tcctgctact ttgtccgcct ccatccagtc tatgagctgc
     5761 tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt tccgaagagt tgtggccatt
     5821 gctactggca tcgtggtatc acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc
     5881 cagcggtcaa gccgggtcac atgatcaccc atattatgaa gaaatgcagt cagctcctta
     5941 gggcctccga tcgttgtcag aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca
     6001 gcactacaca attctcttac cgtcatgcca tccgtaagat gcttttccgt gaccggcgag
     6061 tactcaacca agtcgttttg tgagtagtgt atacggcgac caagctgctc ttgcccggcg
     6121 tctatacggg acaacaccgc gccacatagc agtactttga aagtgctcat catcgggaat
     6181 cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat tgagatccag ttcgatatag
     6241 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt
     6301 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg
     6361 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc
     6421 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
     6481 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     6541 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     6601 aagtgcaggt gccagaacat ttctctggcc taactggccg gtacctgagc tcgctagcct
     6661 cgaggatatc gataaggatc cg
//
