LOCUS       sequence-33082-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP618
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(59..92)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccat
       61 aacttcgtat agcatacatt atacgaagtt atcccgggta ccgagctcga attcaacgaa
      121 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
      181 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
      241 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
      301 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
      361 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
      421 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
      481 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
      541 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
      601 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
      661 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
      721 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
      781 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
      841 tactagagat aaacataaaa aatgtagagg tcga
//
