LOCUS       sequence-33083-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP622
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgactctgag gatccccggg ataacttcgt atagcataca ttatacgaag ttattancca
       61 ttattttttt cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc
      121 gatgactgga aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact
      181 gaaaaattgg gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag
      241 agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat attatttaag
      301 gacctattgt ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct
      361 tttacatttc agcaatatat atatatattt caaggatata ccattctaat gtctgcccct
      421 aagaagatcg tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt
      481 aaggttctta aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat
      541 ttaattggtg gtgctgctat cgaagctaca ggtgtcccac ttccagatga ggcgctggaa
      601 gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtaca
      661 ggtagtgtta gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc
      721 aacttaagac catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca
      781 caatttgcta aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt
      841 ggtaagagaa aggaagatga tggtgatggt gtcgcttggg atagtgaaca atac
//
