LOCUS       sequence-330858-       14825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins (except TniQ) with crRNA 
            targeting AvPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14825)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 14825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS2: pHelper(AvCAST)_AvPSP1_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..14825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     559..578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(598..675)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        721..739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    740..764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        1025..1043
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1044..1068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5047..5094
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5243..5261
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5262..5286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10115..10133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10134..10158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12147..12194
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12396..13187)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13188..13279)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(14340..14825,1..597))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
       61 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
      121 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
      181 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
      241 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
      301 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
      361 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
      421 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
      481 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
      541 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
      601 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
      661 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaaat
      721 taatacgact cactataggg gaattgtgag cggataacaa ttcccctgta gaaataattt
      781 tgtttaactt taataaggag atataccatg gtgctttaac attagatgtc gttaggcgtt
      841 gagcagagaa gtcatttaat aaggccactg ttaaacgtag atgtgcttta acattagatg
      901 tcgttaggcg ttgagcagat ccgaattcga gctcggcgcg cctgcaggtc gacaagcttg
      961 cggccgcata atgcttaagt cgaacagaaa gtaatcgtat tgtacacggc cgcataatcg
     1021 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc atcttagtat
     1081 attagttaag tataagaagg agatatacat atgattattg atagtaactt agttacgcag
     1141 cttccctcag aatcatcttc aaagccaaga gagcttttaa tgtctcagtt tccatatatg
     1201 gatttaactt ttagccttac gggtgagaca ttacctttcg accacggcta tgaattattt
     1261 tctgcgatcg cccactttga acccaaacta catacattag acacgttggg aattcagacc
     1321 attgcaggta ttcccaagga tggtgtaatt aatctgaccc aaaattctag gcttcgtatt
     1381 cggataccag taaatcaggt acgtttggtt tatccattgg cgggtaagtc actgagaatt
     1441 ggcaagcata caattcgttt gggtattcct gacatctgtt tgcttcaacc tgcggagaaa
     1501 ctgcgatcgc gcattgtggt aataagaggc tatgaagaac ctgaaagttt tttagtcgta
     1561 gcgcagcgtc agctagaaca actgggtatc caagcaattg ctagcattcc caccaaagcc
     1621 aatggcaaac caatacgtag gactatcaaa attaagcgat ttacagtcgt gggttttgga
     1681 ttagaagtta taaatttaag cgatgaagac tcactgaggt tgcaaataca tggtgtagga
     1741 ggaaagcaaa agatgggttg tggactcttc atgcctatta aggaaaggca atagcaatta
     1801 ctcgaaagca gatgatgaag agagcagata accccaaaat tcagcttaat ctcggcgatc
     1861 ctagtatgac tttattgcat cgtgctgggg ttgcaggact ttggatgacg ctcaaaaaac
     1921 tggaaaaaat ctatccaaca cctgctgaac gtattggtaa tttaacttgg ttgctaaccc
     1981 ctcactatat caatcttgat tgggaaggac aggatttcac agttttagac tggttgctga
     2041 agcaatcatt tcaaatcagc gatgagggtt taatttccct gacagggtta gattctcaaa
     2101 acatggatat tcaaaatcaa ataattatcc atcaaggtat cacagccact tttacacaac
     2161 ataataaatt ttttaagtct gcgggtcaac aatctaagca actaattatt gatggtattc
     2221 aggttagggt tgattataag aaagctgctt cctatgctca tcaacatttt gctaaacatt
     2281 tatgtgacca gtacggacaa ttgctaaaag agccaattgg tatcagggga tggctctatc
     2341 caggagcagt agttcgccat tatgctttta aagaacaaac agcatttgaa gaaaaagcag
     2401 aatatgctct agcgttgttg ttcgctccta tggcttgtca atattttgtg ctgcgatcgc
     2461 acaccgaacc atcacataca aactatgttt tggttattcc tgaagtaatt gatttagaac
     2521 tttatgcaca ttttttgtgg tgtttgggta acttagatta taaacatttt cacgtatcta
     2581 gcttaggaga tgctggatta aaatttctta attataaaac aacacaaatg cctacttcca
     2641 attttttaaa acgatgtcaa ataatctttt ttagtacaaa agcttggtca gaacagcaaa
     2701 aaactcgtgc agaaatagca atcatagaat taaattcttc agttaattat ctttataagc
     2761 ttagttgtat ttgttttcca gaatgtcaaa ttgttttata taaaaatcaa cattatttgc
     2821 ttccaagttt aataaaagga ataatcgcca ataatctagc aatgggtcgg tcttggtggg
     2881 ctaattggat ttcatttatt aaaaataaaa aatcatttaa acaatttact gatgagtata
     2941 aaggaattaa taagatgatt caggactcag aatgggatat agaagctcaa aagctattta
     3001 ttcaagcttg tcatgaagca ttaagaaaaa tatatgccaa aatttatagc agaacaaaag
     3061 aaggtgaata cgctcaaata gaacgagaaa atacacgcat tttatctcag ttaaaacgct
     3121 gcaccaatac tgacaatttc cgaaaattta ttgcagagtt ttggggaagg gcagggcaaa
     3181 tagcaattct ggaagaacat tggatggaat tacttccatt gactacaggg ataggcaatt
     3241 ggaaagtggc cagggactta acttttattg caatggctag ctacccaaag aataaaataa
     3301 cagaggtaga ggtaaaacca tcacaaatat cggagctaaa tactgaataa acttcatatt
     3361 ttttgatttg attaagaaca aaatattaat tgtttaggta atggtggaat gtttcatctg
     3421 tttggtaata ttttgactag ttatggaacc gccgctaata atcgtggtga aaatgaaggt
     3481 aataccacta cgttacaaaa attaatttgg aaaggtgaag ttcactctac tgtttcatct
     3541 gaggcaattc gttgggcatt gcgatattat tggcaaaatg ctggctatcc tgtgaataga
     3601 cgttgggatg aaaatgctca accagtaccc gatcacatct tgcaagatcc taattttgat
     3661 gatattcgct tcattgatga tgatgtcttg ggatttatgc aagcggaagc agcaaaagtt
     3721 gaagcagcag ttgaatccga agttgaaatc cagaactcaa ctcaagatga ggacaaaaaa
     3781 ctttctcaag gtaaggagaa agaaactgca aaacgtaagc gtaaggaaaa acctaaaggg
     3841 aaaataacta ctagacgagg tgtattagaa gtcactcgtg cagtttcaac aattccttat
     3901 gctggtgatc ttactttcaa tgctatgagt ggaaagaaag gacgaacatc tctttattct
     3961 acagaagttc atgctactcg atatcaatat ggcttcgcat taacacccaa tagcctcaaa
     4021 gataaatctc gcattaatgc tgtcctcgat agcttaattt ctattggaga agtggcagga
     4081 aatcacgctc gttttctcta tgatttttct cctgatagca tcatattacg gtggacacac
     4141 gatttctcac ctcggttact ttactgtttt gaagaagatg aacttagaaa tatatcaaca
     4201 ccggatttag taaggcgggt agaatctgga gatatagacc ctaaagaatt atggatagga
     4261 ggggcaattt ctagcactct ggaagattta ggggcaaatg tgtttcccgg agtgaagaca
     4321 acagttgaag cattaaaaca ggtgattatt gaagatttgc agttgtcgtt aaggagttca
     4381 tgatatgacg acaattgcct taaaagtaga agttcccata gcttgttttc ggcagtctcg
     4441 tgctagggaa tatggcgaaa cttaccctgt accaccacca tcgacagtat atggaatgtt
     4501 actatctctt gttggagaag tggatagata caaacactgc ggtgtgaagc tggcgatcgc
     4561 acttctatct gaaccagaaa agtcaactgt tattcgcacc tttcaccgat ttaagacgaa
     4621 gaatatccat gactctaaaa ataataaacc tgattatcag gagttattaa ctaatataga
     4681 atttatagtt tgggtggatg ctggagtaga taaagctaaa ccaaatttag tggaacgttt
     4741 agcagaggca ttgactaacc cggcttctat taataggttt ggtggattat gtttaggtga
     4801 aagtcgggat ttagtgaatg atgttaccct tttacctaaa aattactatg cagaatcaat
     4861 gcgatggtta attcgagatg aatatgggtt actaacttta ccttactggg tagaccatgt
     4921 aggttcgcgg ggaacgcgat ggctacgtta cgagattcag aaatgtccag tctttcaacc
     4981 acctgaatta tcttggactt cagttcaaag taattagcct aggctgctgc caccgctgag
     5041 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     5101 cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac
     5161 cagcaataga cataagcggc tatttaacga ccctgccctg gatctcgacg ctctccctta
     5221 tgcgactcct gcattaggaa attaatacga ctcactatag gggaattgtg agcggataac
     5281 aattcccctg tagaaataat tttgtttaac tttaataagg agatatacca tggttagggg
     5341 tagaagggaa tggactcaag ctaaatatga gcgatatata aaagaaggtc gtggccgagg
     5401 aagtggtaag cactataaac cttggcttac aatccaagat gttccctcta aaggacgttc
     5461 ttctagaact cctggttgga aaacaaatcg agtacatcat ttcttctcag accatgaaaa
     5521 acggctattt tatttgtttg agtggtcaga tatagtcaca gatataagag agcaatttcc
     5581 tttagacaat ctcgatctag ccatgagtat tgctacagac ataggtataa agtaccctgt
     5641 agatacacaa agtggcactc ctcacgtttt aacaagcgac tttatgctta cagtcaaccg
     5701 cgatgggaag cagatgcaga ttgcccggac tgttaagccg tcaacagaac tggagaaaaa
     5761 gcaagttaat gagaaattcg agatagaaag acaatattac ttagcaaagg gtattgactg
     5821 gggaattatc acagaaaaag aaattcccag gcttttagct gaaaatgttg agtgggttca
     5881 ttctgcttat aggctagaag caaattctga aatggacact gcacaactga gtgaattgtc
     5941 aacaatttta aaatctaagc ttcaggaaga agatacaact ataaatagag taactactgc
     6001 tttagacaaa gaaatgaata taaattccgg tacatcactt tatgtattta aacatttaat
     6061 tgctaggaaa gaaatccttg tggatctagt agcaataaaa ttatctgctt gtccgtcaac
     6121 taaagttatt gaaaaaataa tattttagac ccacatgata ggtaaataat atggttaaag
     6181 atttatttgt caatgattta atagagtgga ttgtatacca aaacactcat atagtcgaaa
     6241 gaattatttg gatagatgaa ggttacatta ttgcatttgt tatagatatt aatgctaaga
     6301 aaggtctacc tgaaccaagg aaaatatcag atattttaga agctataact gaaggtattg
     6361 cttttaagca gcaacaagat ccttgggcaa gaattattcg ggatgaaaat ttgacagata
     6421 aggaaaagga atacagagat aaagcttggg agataatttc atcattaatt atagaagaac
     6481 catcaatata ttatagaaat tttagaggat cgatggttaa agaagcggtt gaaaagtaca
     6541 acgaagaaag ggataaagat aaattagtag aaaaaaccgt ctatggttat ttgagaagat
     6601 tttggcagag aggaaaaact ataaatgctc tcatacctga ttttatcaat tcatcaggta
     6661 agggcaagat tcgaggacaa tcgggcaaga agagaggaag accaagaaaa tatgcacatg
     6721 acccagatat tggtgaaggt gtaaatgtta cagaagaaga tagaaagatt tttagagtat
     6781 ctataagcaa attttacaat aatccaaaag aaaactactt aacaacagcc tatgaattaa
     6841 tggtgaaaga atattacaag gaaggaatta gatatgacga gaatggtgtt caaaaatcta
     6901 tattaacccc aactgataaa atacctacaa ttactcaatt caaatactgg tatcaacttg
     6961 aacaggatga tgttaccaaa actttaacat ctcgaaaagg agccaagcga gtagctttag
     7021 agaatagagc aattttggga aactctaaga tggaaactat tggccctggt tctagatatc
     7081 aaattgatgc cacagttgct gatgtatatt tagtttcaaa atataaccgt gactggataa
     7141 ttggtagacc agttatctat gtaattattg atgtttttag tagaatgatt acaggtgttt
     7201 atgtcggttt agaaggccca tcttggatag gagcaatgat ggcactagcc aatgtagcta
     7261 cagataaaac cctgttttgc caggaatata acattaatat tactgaaaat gaatggcctt
     7321 gcaatcaaat gccagatgca attttaggag ataggggaga attagcaggt atgacagtag
     7381 aaacattaat ccctaatctt tatgttcgta ttgaaaatgc agcttcctat agagcagatt
     7441 ggaaagggtt agtagaaaga cactttcgta taattcatga atatgtaaaa ccttttttac
     7501 ctggttatgt ggatacggat tttaggcaaa gaggtgcaag ggattatagg cttgatggca
     7561 aacttgatat agatcaattt actgagatta ttattcgttt aattgttcac cataacaata
     7621 aacctcttac tgaatatgaa agagatgaag caatgattgc tgatgatacc cctcctatac
     7681 ctagagagtt gtggaagtgg ggtatcgcca atcgttctgg cagactcaga actttttctg
     7741 aagatattgt caaattgaac ttgatgccta ctgagaaagc aactattact gctaaaggca
     7801 tcaaaatgag aggtaaggag atgtactata cctgtgatag tgctaaaaat gagcaatggt
     7861 ttgaaaaagc tagaagtaat ctcctttcta agtcagaaaa atcattgatt gtatcttatg
     7921 acatcagaaa gccaaacttc atttatcttc catctagtga tggtagagat tttgaaaaat
     7981 gttttcttat tgacttagaa gggcggtatg ctaataaaaa ctttcatgat attgaatatc
     8041 tgcttgctta tgaagaattg caaagacaga aaagccaagg taaacagcta caagaaaagg
     8101 tagatttaat agctgatatt gaaagcatag tagatagagc agaaaaaatg actgatgctt
     8161 ctcaatatca taatttaagt aagagcaaga aagtttctgg aataagagat aatagggctt
     8221 ttgaaaaggc taaacgacga gagaatgaag gatttgaatt agcaattaca gataatcaga
     8281 gcagttttca ggttgcagag agtacagcag aaacctccga acagccagaa tcactacagc
     8341 caaaccatct gaatcttctt aagaaaaagc gacaggagcg aaaacgtgga cagaattgag
     8401 agtgaactgg ttaaaattcc taatggagaa ctagcagtta ttgccagata tatagatcaa
     8461 aaactgccag aatacaacac taatccatta attcaggcat tacctcctat cctatcagca
     8521 gcagaattta tagataaagt agccagagaa ccaaattttg atgaacaaga gagagagcta
     8581 gaaccacatt atagatttca ttgtattgag aggttatcta gatattttga ccctcaaaat
     8641 aaaactcttg aattacaaaa agttatttgt acgcttatca tgacaggcta tctgtcaagg
     8701 aaccttttaa aacctgaata tgcaaatcgc tctaggcaaa tttataatgc aataggtaat
     8761 ggaggaggta aaaatttaga aaactatgtt aatcttccta catctgcgtc cggcttaact
     8821 ctaattggcc catcaggtat tggaaagact acaaatttca aaaatattct tgagctttat
     8881 ccccaaataa tttttcatcc tgattatagt gtttaccaaa tagtttggct gcgggtagat
     8941 tgccctaaaa ctttaaaggg gttatgtata gatacttttg caggtatcga taaattgtta
     9001 ggaactaatt tattaaagaa atttaaccca gcacgcacat ctgaagatta tctattggct
     9061 caacttgcac aaacagcaca cactcatcat ctaggagttt tggtgattga tgaaatacaa
     9121 aatttagcaa ctgcaaaaag aaatagagac gaaatattaa attttttagt aaagctcgat
     9181 aatactattg gaattccagt tattcgtgta ggtacgaatg aagccattag ttttcttcaa
     9241 ggtaatctta gaaatgctag aagaaatact ggagaaggaa gtagaatgtg ggatagaatg
     9301 gtaaataatg atgaatggtg ttacttttta gaaggaatat gggaatacca atggacaaaa
     9361 acacctgttc atttctcgga tgaaataaaa gaagtttttt atgatgaaag ccagggaatt
     9421 attgatattg ctataaaact ttacaaaatg gttcaatgga gagcgatttc aataggtgac
     9481 caagaaatta ttacggttga tctgattcgt caagtagcta aagaaggatt gtatttagta
     9541 aagccaatgc tagatgcaat taggttaggg gacagagagt ggatgattaa atataaggat
     9601 attgctcctc tcgatattac ggaatatcac aatagatgtg tatctgagat tgatgctaaa
     9661 gatttgacag agattaagag attagctaaa aagcagcaga atcagcagaa agtatctccg
     9721 aagctcaatt atgtaattag tgagctgttg aaattggaag tagaatctgg taaagcaaag
     9781 gaatgtgcag aacaaattgt ttatagtcat gaagatccag acattccaat tccatcttta
     9841 gttaataagg catatgctct tgctttgcaa ggatgtcaag taaatgagag tcttgctaaa
     9901 acaagtacca ggaaagctaa attaaagaca aagtaccaag agaatgatat tcggcgaatt
     9961 gtcgagaatg ccaagaaaga caaaaaatct gcatacgagg atttgaaatt agcaggtata
    10021 atcaaaaatc cagttgaaga ttttatcaat atttagcttc taacttaagt cgaacagaaa
    10081 gtaatcgtat tgtacacggc cgcataatcg aaattaatac gactcactat aggggaattg
    10141 tgagcggata acaattcccc atcttagtat attagttaag tataagaagg agatatacat
    10201 ggtgagtttt ttccctcaac cttatccaga tgaaatttta tatagtgtta ttgctcgtta
    10261 tcacattaga agtggcaata cgagtccaaa aattacatta caagatttat ttaagtcaca
    10321 gactactgtg gcgacagtag atttaccatc gaaccttagt aatttaatta aaaacctaca
    10381 atttatctca aattaccaag ttgaagagtt gatatataaa catactctct accctttata
    10441 tagccctttt ttgccagcaa acaggtctag tcaggtactg gaatctatga aaggagatta
    10501 tgggggagat attcatacaa gagccggaat tatggcaagc tcaattacta tgccgaaata
    10561 tttgcggttt tgtccaactt gctgggaaga agacttaaaa aattatggcg aaccttactg
    10621 gcatcgcatc catcaaatac cagggatttt agtttgtcca tttcatggtg aagttttgca
    10681 agatagccta gctcctttac atggttttaa taagcatgaa tattatgcag ctagtacaga
    10741 caactgttct attactaaag aacccagtat tcttagccct gacacactga aaaaattact
    10801 aatattagcc aaagattttt ggtggttact caatagtgaa ataccttgcc aagagccaca
    10861 gtggtttcgc aaacagtaca caaatctgat gattgagcag ggttttgcaa ctccaactgg
    10921 tcgaatttac cagaacaaac tactagataa ttttttgttc ttttatggtc aagaaatgct
    10981 cagtgctgtt gattcaatgg taaatcataa tgattcaaac aactgggtta ctagcatcgt
    11041 caggaaatat aggaaatctt tccatcctct tagacatctg ctaatgatgc gttttttgaa
    11101 aacatcagta gaggaatttt ttcaaaaaga tactgaatac aagccttttg gcgaaccccc
    11161 ctggttgtgt ctcaatgctg ccgcagaaca ttatctacaa ccagtgatta ccaatttagt
    11221 cattacttta tgctgcgata caaaaaagcc tgtcggtact ttttcttgtg cttgcggaat
    11281 ggtttattgc cggactgggc ctgatgaaac ggatgaagac aaagttcgca ttggtaaagt
    11341 caaggcattc ggtcaaatat gggagcaaaa gctgaaagaa ttggtggaag ttgaaaagtt
    11401 gggtttgcgg gagacagcca gaagattaaa tgttgatgcc agaactatca aacgttatgt
    11461 atctcgtcta aaattgacaa cttattggca gactagaaca gaaaatgact ctgtaggctt
    11521 acagggagtc ccagaaatag atcccaattt tgcaactgaa ctcaaattaa aacacaggca
    11581 aaattggatg gctttacaag tacagaaccc tgaagcttca aagacaactc tgagaaaact
    11641 agtaccagcc acctatattt ggctatacag gaatgacaga gaatggctca atcaaaactc
    11701 acccacttta caagttgccg ttccttccgt tgtgagagtt gattggcatg agcgagatca
    11761 gcaaattctc gctcaggttc aagatgcagt gcgatcgcta cttaaggctg atataccaga
    11821 gaaaatttct attagtaggg tagggaaaac tatcggctta cttgcattac tcgaaaaaca
    11881 tctcgaccag atgcctttaa caaaggctta tttggaatca gtcgttgaaa ctgtagaaga
    11941 atttcagatg cgccggatca agtgggcaat caagctgctt gatgaatgtg gagaagaaat
    12001 tatgcgctgg aaggtagtca gagttgctgc tttgcgagag gattgctccc aaagagtcag
    12061 tgcatttctg gaaagtgaac tggagaaagc atacaagaaa gagaggtgat taattaacct
    12121 aggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
    12181 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
    12241 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
    12301 aaccgacgac aagctgacga ccgctgccct gaaccgacga ccgggtcatc gtggccggat
    12361 cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
    12421 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
    12481 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
    12541 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
    12601 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
    12661 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
    12721 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
    12781 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
    12841 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
    12901 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
    12961 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
    13021 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
    13081 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
    13141 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatact cttccttttt
    13201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13261 atttagaaaa ataaacaaat agctagctca ctcggtcgct acgctccggg cgtgagactg
    13321 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
    13381 aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
    13441 ctgccagagt tcacataaac agacgctttt ccggtgcatc tgtgggagcc gtgaggctca
    13501 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
    13561 gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc
    13621 cgcctttctc ccttacggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg
    13681 ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga
    13741 ctgctgcgcc ttatccggta actgttcact tgagtccaac ccggaaaagc acggtaaaac
    13801 gccactggca gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg
    13861 cggttgctct tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct
    13921 gtgctctgcg aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat
    13981 caaaccacct ccccaggtgg ttttttcgaa ggttaagtca gttggggaac tgcttaacct
    14041 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
    14101 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgctcta gatttcagtg
    14161 caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa gttgtaattc
    14221 tcatgttagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg
    14281 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct
    14341 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    14401 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
    14461 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
    14521 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
    14581 catgagctgt cttcggtatc gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc
    14641 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
    14701 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
    14761 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
    14821 cagcc
//
