LOCUS       sequence-330864-        9007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human GPT2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 9007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC2-GPT2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1833..1853
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2142..2345
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2296..2316
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3544..3553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3988..4053
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4101..4218
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4267..4777
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4696..4714
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4799..5398
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4799..4818)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5295..5315
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5526..5759
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5831..5952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5868..5887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5922..5941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5992..6127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6147..6166)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6148..6166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6176..6193)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6176..6192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6185..6207)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6334..6789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6421..6440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6631..6652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6815..6919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6920..6988,6989..7780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7138..7157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7951..8539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8440..8459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8693..8710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8827..8848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8863..8868,8869..8886,8887..8893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8901..8917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8906..8928
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8925..8941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8925..8941
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8960..8980
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8962..8980
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcagca gcccccttca ccgagggcct atttcccatg attccttcat
     1861 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     1921 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1981 ttttaaaatt atgttttaaa atggactatc atatgccaag tacgccccct attgacgtca
     2041 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2101 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2161 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2221 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2281 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2341 gagctggttt agtgaaccgt cagatccgct agcgctaccg gtttaattaa gcgcgcgatg
     2401 cagcgggcgg cagcactggt caggcgaggc tgtggtcctc ggactccaag ctcctggggc
     2461 agaagccaga gtagcgctgc tgccgaagcc tcggcggtgc tcaaggtgcg gcccgagcgc
     2521 agccggcgcg agcgcatcct cacgctggag tccatgaacc cgcaggtgaa ggcggtggag
     2581 tacgccgtgc ggggacccat cgtgctcaag gccggcgaga tcgagctcga gctgcagcgg
     2641 ggtatcaaaa agccattcac agaggtcatc cgagccaaca tcggggacgc ccaggctatg
     2701 gggcagcagc caatcacctt cctccggcag gtgatggcac tatgcaccta cccaaacctg
     2761 ctggacagcc ccagcttccc agaagatgct aagaaacgtg cccggcggat cctgcaggct
     2821 tgtggcggga acagcctggg gtcctacagt gctagccagg gtgtcaactg catccgtgaa
     2881 gatgtggctg cctacatcac caggagggat ggcggtgtgc ctgcggaccc cgacaacatc
     2941 tacctgacca cgggagctag tgacggcatt tctacgatcc tgaagatcct cgtctccggg
     3001 ggcggcaagt cacggacagg tgtgatgatc cccatcccac aatatcccct ctattcagct
     3061 gtcatctctg agctcgacgc catccaggtg aattactacc tggacgagga gaactgctgg
     3121 gcgctgaatg tgaatgagct ccggcgggcg gtgcaggagg ccaaagacca ctgtgatcct
     3181 aaggtgctct gcataatcaa ccctgggaac cccacaggcc aggtacaaag cagaaagtgc
     3241 atagaagatg tgatccactt tgcctgggaa gagaagctct ttctcctggc tgatgaggtg
     3301 taccaggaca acgtgtactc tccagattgc agattccact ccttcaagaa ggtgctgtac
     3361 gagatggggc ccgagtactc cagcaacgtg gagctcgcct ccttccactc cacctccaag
     3421 ggctacatgg gcgagtgtgg ttacagagga ggctacatgg aggtgatcaa cctgcaccct
     3481 gagatcaagg gccagctggt gaagctgctg tcggtgcgcc tgtgcccccc agtgtctggg
     3541 caggccgcca tggacattgt cgtgaacccc ccggtggcag gagaggagtc ctttgagcaa
     3601 ttcagccgag agaaggagtc ggtcctgggt aatctggcca aaaaagcaaa gctgacggaa
     3661 gacctgttta accaagtccc aggaattcac tgcaacccct tgcagggggc catgtacgcc
     3721 ttccctcgga tcttcattcc tgccaaagct gtggaggctg ctcaggccca tcaaatggct
     3781 ccagacatgt tctactgcat gaagctcctg gaggagactg gcatctgtgt cgtgcccggc
     3841 agtggctttg ggcagaggga aggcacttac cacttcagga tgactatcct ccctccagtg
     3901 gagaagctga aaacggtgct gcagaaggtg aaagacttcc acatcaactt cctggagaag
     3961 tacgcgggag gcagcgcggc cgcaggcgac tacaaagacc atgacggtga ttataaagat
     4021 catgacattg attacaagga tgacgatgac aagtaataag aattctcgac ctcgagacaa
     4081 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     4141 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4201 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
     4261 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     4321 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     4381 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     4441 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     4501 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     4561 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     4621 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     4681 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     4741 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat
     4801 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4861 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4921 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4981 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5041 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5101 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5161 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5221 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5281 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5341 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     5401 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc
     5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5521 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5581 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5641 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5701 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5761 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     5821 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5881 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5941 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     6001 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6061 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6121 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     6181 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6241 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6301 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6361 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6421 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6481 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6541 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6601 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6661 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6721 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6781 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6901 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6961 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7021 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7081 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7141 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7201 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7261 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7321 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7381 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7441 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     7501 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7561 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7621 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7681 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7741 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7801 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7861 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7921 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7981 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8041 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8101 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8161 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8221 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8281 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8341 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8401 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8461 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8521 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8581 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8641 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8701 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8761 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8821 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8881 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8941 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     9001 agcttaa
//
