LOCUS       sequence-330871-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CPSF6 fused to eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, 
            Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
  TITLE     Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection 
            in a Cyclophilin A-Dependent Manner.
  JOURNAL   mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 
            10.1128/mBio.03142-20.
  PUBMED    33758083
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-23"; volume: "12(2). pii: mBio.03142-20. doi"; pages:
            " 10.1128/mBio.03142-20"
COMMENT     Sequence Label: pSICO-CPSF6-eGFP
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    209..334
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    831..1064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1249..1293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1591..1708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1998..2018
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2036..2069)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        2169..2472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2473..2676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4554..4563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4560..4562,4563..4565,4566..5279)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4560..4562,4563..4565,4566..5276)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4605..4626)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4866..4885)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5213..5234
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5287..5320)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     5347..5363
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5438..6026
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5491..5511)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5909..5920)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6029..6045)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6555..6735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6797..7385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6877..6896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7556..8347,8348..8416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8179..8198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8417..8521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8596..8615)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8787..9166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9168..9366
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             join(9384..9402,1..162)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
       61 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      121 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      181 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      241 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      301 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      361 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
      421 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
      481 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
      541 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
      601 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
      661 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
      721 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      781 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      841 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
      901 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
      961 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1021 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1081 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1141 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1201 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1261 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1321 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1381 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1441 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1501 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     1561 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     1621 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     1681 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     1741 ttggttagta ccgggcccgc tctagagatc cgacgcgcca tctctaggcc cgcgccggcc
     1801 ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat
     1861 aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt
     1921 ttaatactag ctacatttta catgataggc ttggatttct ataagagata caaatactaa
     1981 attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag taattataac
     2041 ttcgtatagt ataaattata cgaagttata agccttggtt ttttgaattc cgtattaccg
     2101 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2161 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2221 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2281 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2341 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2401 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2461 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2521 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2581 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2641 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcggatccca
     2701 gtgtggtggt acgggaattc aggaagatgg cggacggcgt ggaccacata gacatttacg
     2761 cggatgtcgg cgaagagttc aaccaggaag ctgaatatgg tgggcatgat cagatagatt
     2821 tgtatgacga tgtcatatct ccatctgcaa ataatggaga tgccccagaa gaccgagatt
     2881 acatggatac tctcccacca actgttggtg atgatgtggg taaaggagca gcaccaaatg
     2941 ttgtctatac atatactgga aagagaattg cattatatat tggaaatcta acatggtgga
     3001 caacagatga agacttaact gaagcagttc attctttggg agtaaatgat attttggaga
     3061 taaaattttt tgaaaatcgg gcaaatggcc agtcaaaggg gtttgccctt gttggtgttg
     3121 gatctgaagc atcttcaaaa aagttaatgg atctgttacc taaaagagaa cttcatggtc
     3181 agaatcctgt tgtaactcca tgcaataaac agttcctgag tcaatttgaa atgcagtcca
     3241 ggaaaactac acaatcagga caaatgtctg gggaaggtaa agctggtcct ccaggaggca
     3301 gttcccgtgc agcatttcca caaggtggta gaggacgggg ccgttttcca ggggctgttc
     3361 ctggtgggga cagatttcct gggccagcag gaccaggagg gccaccccca ccttttccag
     3421 gaaatttgat caagcatctt gttaaaggaa ctcggccttt gttcctggaa actaggattc
     3481 catggcatat ggggcacagc atagaggaaa tacccatttt tggcctaaaa gctggacaga
     3541 ctccaccacg tccaccctta ggtcctccag gcccacctgg tccaccaggt cctccacctc
     3601 ctggtcaggt tctgcctcct cctctagctg ggcctcctaa tcgaggagat cgccctccac
     3661 caccagttct ttttcctgga caaccttttg ggcagcctcc attgggtcca cttcctcctg
     3721 gccctccacc tccagttcca ggctacggcc cccctcctgg cccaccacct ccacaacagg
     3781 gaccacctcc acctccaggc ccctttccac ctcgtccacc cggtccactt gggccacccc
     3841 ttacactagc tcctcctccg catcttcctg gaccacctcc aggtgcccca ccgccagctc
     3901 cgcatgtgaa cccagctttc tttcctccac caactaacag tggcatgcct acatcagata
     3961 gccgaggtcc accaccaaca gatccatatg ggcgacctcc accatatgat aggggtgact
     4021 atggcccccc tggaagggaa atggatactg caagaacgcc attgagtgaa gctgaatttg
     4081 aagaaatcat gaatagaaat agggcaatct caagcagtgc tatttcgaga gctgtgtctg
     4141 atgccagtgc tggtgattat gggagtgcta ttgagacact ggtaactgca atttctttaa
     4201 ttaaacaatc caaagtatct gctgatgatc gttgcaaagt tcttattagt tctttgcaag
     4261 attgccttca tggaattgag tccaagtctt atggttctgg atcaagacgt gaacgatcaa
     4321 gagagaggga ccatagtaga tcacgagaaa agagtcgacg tcataaatcc cgtagtagag
     4381 accgtcatga cgattattac agagagagaa gcagagaacg agagaggcac cgggatcgtg
     4441 accgagaccg tgaccgagag cgtgaccgag agcgcgaata tcgtcatcgt gaggtaccgc
     4501 gggcccggga tccgctagcg ctaccggttg cggatccgct agcgctaccg gtcgccacca
     4561 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     4621 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     4681 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4741 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     4801 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4861 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4921 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4981 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     5041 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     5101 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5161 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5221 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtagc
     5281 ggccgcataa cttcgtatag tataaattat acgaagttat aagccttgtt aacgcgcggt
     5341 gaccctcgag gtcgacggta tcgataagct cgcttcacga gatcatgttt aagggttccg
     5401 gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     5461 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5521 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5581 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5641 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5701 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5761 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5821 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5881 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5941 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6001 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     6061 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6121 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6181 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6241 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6301 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     6361 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     6421 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6481 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     6541 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6721 ggaaaatctc tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7201 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7861 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7921 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7981 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8041 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8101 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8161 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8221 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8281 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8341 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8401 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8461 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8521 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8581 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8641 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     8701 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     8761 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     8821 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     8881 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     8941 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     9001 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     9061 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     9121 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     9181 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     9241 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     9301 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     9361 aagcagcgcg ttttgcctgt actgggtctc tctggttaga cc
//
