LOCUS       sequence-330876-        9210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of HA-TCAB1 (WDR79) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, 
            Lin S, Lee B, Pech MF, Venteicher AS, Das R, Chang HY, Artandi SE
  TITLE     An Activity Switch in Human Telomerase Based on RNA Conformation and
            Shaped by TCAB1.
  JOURNAL   Cell. 2018 Jun 28;174(1):218-230.e13. doi: 
            10.1016/j.cell.2018.04.039. Epub 2018 May 24.
  PUBMED    29804836
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.04.039"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "218-230.e13"
COMMENT     Sequence Label: pMGIB-HA-hTCAB1
FEATURES             Location/Qualifiers
     source          1..9210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    478..835
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             908..1324
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1254..1276
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1292..1308
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1638..1654
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     promoter        1684..1702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1763..1772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1784..1810
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1814..1840
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1844..1870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3643..4104
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3699..3716)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3926..3945
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4844..5313
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6009..6026)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6009..6025)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6018..6040)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6234..6253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6353..6375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6413..6431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6499..6603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6604..6672,6673..7464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6822..6841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7635..8223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8124..8143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8377..8394
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8547..8552,8553..8570,8571..8577)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8585..8601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8590..8612
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8609..8625
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8609..8625
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             join(9151..9210,1..413)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
       61 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      241 aaaagagccc acaacccctc actcggcgcc gcgccagtcc tccgattgac tgagtcgccc
      301 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
      361 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg
      421 gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag
      481 ctggccagca acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg
      541 cctgcgtcgg tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga
      601 cgagttcgga acacccggcc gcaaccctgg gagacgtccc agggacttcg ggggccgttt
      661 ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg actctttggt gcacccccct
      721 tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
      781 ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca
      841 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat atgggcccgc
      901 cgcggcgggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
      961 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     1021 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     1081 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     1141 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     1201 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     1261 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     1321 cgcccccata tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg
     1381 acatgacaag agttactaac agcccctctc tccaagctca cttacaggct ctctacttag
     1441 tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg
     1501 tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga
     1561 acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca
     1621 aagtagacgg catcgcagct tggatacacg ccgcccacgt aaaacaattg aagatctagt
     1681 cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccaatt
     1741 cgagctcggt acccgtttaa acgccaccat ggggcaacaa ggatacccgt atgatgttcc
     1801 ggattacgct ggctacccat acgacgtccc agactacgct ggctacccat acgacgtccc
     1861 agactacgct caaggagtgc ggccgcgggg gcgcgccaag actttggaga ctcaaccgtt
     1921 agctccggac tgctgtcctt cagaccagga cccagctcca gcccatcctt ctccccacgc
     1981 ttccccgatg aataaaaatg cggactctga actgatgcca ccgcctcccg aaagggggga
     2041 tccgccccgg ttgtccccag atcctgtggc tggctcagct gtgtcccagg agctacggga
     2101 gggggaccca gtttctctct ccactcccct ggaaacagag tttggttccc ctagtgagtt
     2161 gagtcctcga atcgaggagc aagaactttc tgaaaataca agccttcctg cagaagaagc
     2221 aaacgggagc ctttctgaag aagaagcgaa cgggccagag ttggggtctg gaaaagccat
     2281 ggaagatacc tctggggaac ccgctgcaga ggacgaggga gacaccgctt ggaactacag
     2341 cttctcccag ctgcctcgat ttctcagtgg ttcctggtca gagttcagca cccaacctga
     2401 gaacttcttg aaaggctgta agtgggctcc tgacggaagt tgtattctaa caaatagtgc
     2461 tgataacatc ttgcgaattt ataacctgcc cccagagctg taccatgagg gggagcaggt
     2521 ggaatatgca gaaatggtcc ctgtccttcg aatggtggaa ggtgatacca tctatgatta
     2581 ctgctggtat tctctgatgt cctcagccca gccagacacc tcctacgtgg ccagcagcag
     2641 cagagaaaat ccaatccaca tctgggacgc attcactgga gagctccggg cttcctttcg
     2701 cgcctacaac cacctggatg agctgacggc agcccattcg ctctgcttct ccccggatgg
     2761 ctcccagctc ttctgtggct tcaaccggac tgtgcgtgtt ttttccacgg cccggcctgg
     2821 ccgagactgc gaggtccgag ccacatttgc aaaaaagcag ggccagagcg gcatcatctc
     2881 ctgcatagcc ttcagcccag cccagcccct ctatgcctgt ggctcctacg gccgctccct
     2941 gggtctgtat gcctgggatg atggctcccc tctcgccttg ctgggagggc accaaggggg
     3001 catcacccac ctctgctttc atcccgatgg caaccgcttc ttctcaggag cccgcaagga
     3061 tgctgagctc ctgtgctggg atctccggca gtctggttac ccactgtggt ccctgggtcg
     3121 agaggtgacc accaatcagc gcatctactt cgatctggac ccgaccgggc agttcctagt
     3181 gagtggcagc acgagcgggg ctgtctctgt gtgggacacg gacgggcctg gcaatgatgg
     3241 gaagccggag cccgtgttga gttttctgcc ccagaaggac tgcaccaatg gcgtgagcct
     3301 gcaccctagc ctgcctctcc tggccactgc ctccggtcag cgtgtgtttc ctgagcccac
     3361 agagagtggg gacgaaggag aggagctggg ccttcccttg ctctccacgc gccacgtcca
     3421 ccttgaatgt cggcttcagc tctggtggtg tgggggggcg ccagactcca gcatccctga
     3481 tgatcaccag ggcgagaaag ggcagggagg aacggaggga ggtgtgggtg agctgatata
     3541 attaattaag tctagagtcg actgtttaaa cctgcaggca tgcaagctcg acctcgacca
     3601 attccggtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3661 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3721 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3781 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3841 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3901 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3961 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4021 tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     4081 ttcctttgaa aaacacgatg ataataccat gaaaacattt aacatttctc aacaggatct
     4141 agaattagta gaagtagcga cagagaagat tacaatgctt tatgaggata ataaacatca
     4201 tgtgggagcg gcaattcgta cgaaaacagg agaaatcatt tcggcagtac atattgaagc
     4261 gtatatagga cgagtaactg tttgtgcaga agccattgcg attggtagtg cagtttcgaa
     4321 tggacaaaag gattttgaca cgattgtagc tgttagacac ccttattctg acgaagtaga
     4381 tagaagtatt cgagtggtaa gtccttgtgg tatgtgtagg gagttgattt cagactatgc
     4441 accagattgt tttgtgttaa tagaaatgaa tggcaagtta gtcaaaacta cgattgaaga
     4501 actcattcca ctcaaatata cccgaaatta aaagttttac cataccaagc tcatcgggcc
     4561 gccaggtaca agtaaggatc cggattagtc caatttgtta aagacaggat atcagtggtc
     4621 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     4681 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     4741 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     4801 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     4861 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     4921 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     4981 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     5041 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     5101 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     5161 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     5221 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     5281 agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg
     5341 gttttttttc ttaagtattt acattaaatg gccatagtac ttaaagttac attggcttcc
     5401 ttgaaataaa catggagtat tcagaatgtg tcataaatat ttctaatttt aagatagtat
     5461 ctccattggc tttctacttt ttcttttatt tttttttgtc ctctgtcttc catttgttgt
     5521 tgttgttgtt tgtttgtttg tttgttggtt ggttggttaa ttttttttta aagatcctac
     5581 actatagttc aagctagact attagctact ctgtaaccca gggtgacctt gaagtcatgg
     5641 gtagcctgct gttttagcct tcccacatct aagattacag gtatgagcta tcatttttgg
     5701 tatattgatt gattgattga ttgatgtgtg tgtgtgtgat tgtgtttgtg tgtgtgattg
     5761 tgtatatgtg tgtatggttg tgtgtgattg tgtgtatgta tgtttgtgtg tgattgtgtg
     5821 tgtgtgattg tgcatgtgtg tgtgtgtgat tgtgtttatg tgtatgattg tgtgtgtgtg
     5881 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgttgtgt atatatattt atggtagtga
     5941 gaggcaacgc tccggctcag gtgtcaggtt ggtttttgag acagagtctt tcacttagct
     6001 tggaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6061 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     6181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     6241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     6421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7741 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8401 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8461 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8521 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8581 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8641 ctttgctctt aggagtttcc taatacatcc caaactcaaa tatataaagc atttgacttg
     8701 ttctatgccc tagggggcgg ggggaagcta agccagcttt ttttaacatt taaaatgtta
     8761 attccatttt aaatgcacag atgtttttat ttcataaggg tttcaatgtg catgaatgct
     8821 gcaatattcc tgttaccaaa gctagtataa ataaaaatag ataaacgtgg aaattactta
     8881 gagtttctgt cattaacgtt tccttcctca gttgacaaca taaatgcgct gctgagaagc
     8941 cagtttgcat ctgtcaggat caatttccca ttatgccagt catattaatt actagtcaat
     9001 tagttgattt ttatttttga catatacatg tgaaagaccc cacctgtagg tttggcaagc
     9061 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagaaaagt
     9121 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     9181 taagcagttc ctgccccggc tcagggccaa
//
