LOCUS       sequence-33090-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in 
            light-inducible protein interaction modules for targeting to the 
            plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
  TITLE     Rapid blue-light-mediated induction of protein interactions in 
            living cells.
  JOURNAL   Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524
  PUBMED    21037589
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2010-12"; volume: "7"; issue: 
            "12"; pages: "973-5"
COMMENT     Sequence Label: pCIBN(deltaNLS)-pmGFP
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(40..549)
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     promoter        complement(584..787)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(613..633)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctctcgccc ttgctcacca tggtggcgac cggtacatga atataatccg ttttctccaa
       61 ttccttcgtc actttagatg aatcattaga gaaattgttc tcttctttct tggctttgtg
      121 tttcatcttc ttgatgcttt ttgtgctccc attgttttgc tctgttattt tcgacttctc
      181 ttcttctcct tcttctacta ggtcatctct gttcatcgtc atcttcttcg ccgcctcatt
      241 acaatcctta gtctctgtat caaacttcgc tgccttgaaa tttccagtcc caagcgtcgt
      301 ttccggcgaa attgagagtc ttgaatcacc ttccaccgtc gtctcaccgt acatcatcgg
      361 aagattcaaa ccggcagtcg aaagatagct gtccatctcg ccgccggtaa tcattgaaga
      421 atcggcaaag aagccggcga gaggagaatc aaaggtggga ttgaggtact tgaggtgagc
      481 cctttggcgc tctaggaccg acatgtcagg aaaattgagc aaaaggtcac ctcctatagc
      541 tccattcatg gtggcgctag cggatctgac ggttcactaa accagctctg cttatataga
      601 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      661 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      721 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
      781 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
      841 at
//
