LOCUS       sequence-330934-        3258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway attB5r/attB1r entry clone for generation of N-terminal 
            SNAP-tagged fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
  TITLE     Combinatorial assembly of clone libraries using site-specific 
            recombination.
  JOURNAL   Methods Mol Biol. 2014;1116:193-208. doi: 
            10.1007/978-1-62703-764-8_14.
  PUBMED    24395366
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1116:193-208. doi"; pages: " 
            10.1007/978-1-62703-764-8_14"
COMMENT     Sequence Label: C514-E44: attB5r-SNAP-nostop-attB1r
FEATURES             Location/Qualifiers
     source          1..3258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(44..167)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    744..868
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(940..959)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(941..959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(964..980)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(964..980)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1405..2196
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2289..2877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2778..2797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3012..3039)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3131..3217)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agctcgaatt ctacagtcac taataccatc taagtagttg attcatagtg actgcatatg
       61 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
      121 atttatatca ttttacgttt ctcgttcaac ttttgtatac aaagtggacc atggacaagg
      181 actgcgagat gaaacggact acccttgatt cgcccctcgg aaagctcgaa ctcagcggct
      241 gtgaacaggg tctgcacgag atcaagctgc tgggaaaggg cacctccgcc gccgacgccg
      301 tggaagtgcc agctcccgcc gccgtgcttg gaggccccga gccgctgatg caggccactg
      361 cctggctcaa cgcgtacttt caccaaccgg aagccatcga ggaattccct gtgcccgcac
      421 tgcaccaccc ggtgttccag caggagtcct tcacccgcca agtcctgtgg aagctgttga
      481 aggtcgtgaa gttcggcgaa gtgatcagct accagcagct ggcagccctg gccgggaacc
      541 ctgccgcgac cgctgctgtc aagaccgcgc tgtccggcaa cccagtgccg attctgattc
      601 cctgccatcg ggtggtgtca tcctcgggag ccgtgggagg ctacgaagga ggtttggcgg
      661 tcaaggagtg gctgctggca catgaggggc acagactcgg aaagccggga ctggggcctg
      721 ccgggatcgg tgcccctggc tccacaactt tgtacaaaaa agttgaacga gaaacgtaaa
      781 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      841 gtaaaacaca acatatgcag tcactatgaa ccaactactt agatggtatt agtgacctgt
      901 agaattcgag ctctagagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac
      961 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     1021 gcacaagatc ggtacccggg gatcctctag accagccagg acagaaatgc ctcgacttcg
     1081 ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     1141 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     1201 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     1261 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     1321 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     1381 cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa
     1441 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     1501 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     1561 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     1621 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     1681 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     1741 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     1801 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1861 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1921 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1981 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     2041 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     2101 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     2161 gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa
     2221 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2881 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcca ggaagagttt
     3001 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3061 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3121 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3181 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3241 gcgttaacgc taccatgg
//
