LOCUS       sequence-330940-       10886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product
            in frame with a self-cleaving eGFP. APEX biotin ligase can 
            biotynilate RNAs in proximity of the RLR RIG-I sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pLenti-RIGI-Apex-
            T2A-EGFP
FEATURES             Location/Qualifiers
     source          1..10886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1713..1733
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    1952..2069
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2138..2349
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2389..3138
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     CDS             5947..6003
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6004..6006,6007..6009,6010..6720)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6049..6070)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6310..6329)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6657..6678
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6739..7327
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6792..6812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7210..7221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7399..7632
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7710..7831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7747..7766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7801..7820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7871..8006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8026..8045)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8027..8045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8055..8072)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8055..8071)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8064..8086)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8213..8668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8300..8319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8510..8531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8694..8798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8799..8867,8868..9659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9017..9036)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9830..10418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10319..10338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10572..10589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10706..10727
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10742..10747,10748..10765,10766..10772)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10780..10796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10785..10807
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10804..10820
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10804..10820
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10839..10859
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10841..10859
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg attccttcat
     1741 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     1801 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     1861 ttttaaaatt atgttttaaa atggactatc atacattcca tcgatgtagt caattctaga
     1921 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     1981 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2041 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     2101 tttggcgccg gctcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     2161 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     2221 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     2281 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     2341 agaacacagg tgtcgtgacg cgggatcctg tacatacgta ggccggccat gggaaagtct
     2401 tacccaactg tgagtgctga ttaccaggac gccgttgaga aggcgaagaa gaagctcaga
     2461 ggcttcatcg ctgagaagag atgcgctcct ctaatgctcc gtttggcatt ccactctgct
     2521 ggaacctttg acaagggcac gaagaccggt ggacccttcg gaaccatcaa gcaccctgcc
     2581 gaactggctc acagcgctaa caacggtctt gacatcgctg ttaggctttt ggagccactc
     2641 aaggcggagt tccctatttt gagctacgcc gatttctacc agttggctgg cgttgttgcc
     2701 gttgaggtca cgggtggacc taaggttcca ttccaccctg gaagagagga caagcctgag
     2761 ccaccaccag agggtcgctt gcccgatccc actaagggtt ctgaccattt gagagatgtg
     2821 tttggcaaag ctatggggct tactgaccaa gatatcgttg ctctatctgg gggtcacact
     2881 attggagctg cacacaagga gcgttctgga tttgagggtc cctggacctc taatcctctt
     2941 attttcgaca actcatactt cacggagttg ttgagtggtg agaaggaagg tctccttcag
     3001 ctaccttctg acaaggctct tttgtctgac cctgtattcc gccctctcgt tgacaaatat
     3061 gcagcggacg aagatgcctt ctttgctgat tacgctgagg ctcaccaaaa gctttccgag
     3121 cttgggtttg ctgatgccgg atccatgacc accgagcagc gacgcagcct gcaagccttc
     3181 caggattata tccggaagac cctggaccct acctacatcc tgagctacat ggccccctgg
     3241 tttagggagg aagaggtgca gtatattcag gctgagaaaa acaacaaggg cccaatggag
     3301 gctgccacac tttttctcaa gttcctgttg gagctccagg aggaaggctg gttccgtggc
     3361 tttttggatg ccctagacca tgcaggttat tctggacttt atgaagccat tgaaagttgg
     3421 gatttcaaaa aaattgaaaa gttggaggag tatagattac ttttaaaacg tttacaacca
     3481 gaatttaaaa ccagaattat cccaaccgat atcatttctg atctgtctga atgtttaatt
     3541 aatcaggaat gtgaagaaat tctacagatt tgctctacta aggggatgat ggcaggtgca
     3601 gagaaattgg tggaatgcct tctcagatca gacaaggaaa actggcccaa aactttgaaa
     3661 cttgctttgg agaaagaaag gaacaagttc agtgaactgt ggattgtaga gaaaggtata
     3721 aaagatgttg aaacagaaga tcttgaggat aagatggaaa cttctgacat acagattttc
     3781 taccaagaag atccagaatg ccagaatctt agtgagaatt catgtccacc ttcagaagtg
     3841 tctgatacaa acttgtacag cccatttaaa ccaagaaatt accaattaga gcttgctttg
     3901 cctgctatga aaggaaaaaa cacaataata tgtgctccta caggttgtgg aaaaaccttt
     3961 gtttcactgc ttatatgtga acatcatctt aaaaaattcc cacaaggaca aaaggggaaa
     4021 gttgtctttt ttgcgaatca gatcccagtg tatgaacagc agaaatctgt attctcaaaa
     4081 tactttgaaa gacatgggta tagagttaca ggcatttctg gagcaacagc tgagaatgtc
     4141 ccagtggaac agattgttga gaacaatgac atcatcattt taactccaca gattcttgtg
     4201 aacaacctta aaaagggaac gattccatca ctatccatct ttactttgat gatatttgat
     4261 gaatgccaca acactagtaa acaacacccg tacaatatga tcatgtttaa ttatctagat
     4321 cagaaacttg gaggatcttc aggcccactg ccccaggtca ttgggctgac tgcctcggtt
     4381 ggtgttgggg atgccaaaaa cacagatgaa gccttggatt atatctgcaa gctgtgtgct
     4441 tctcttgatg cgtcagtgat agcaacagtc aaacacaatc tggaggaact ggagcaagtt
     4501 gtttataagc cccagaagtt tttcaggaaa gtggaatcac ggattagcga caaatttaaa
     4561 tacatcatag ctcagctgat gagggacaca gagagtctgg caaagagaat ctgcaaagac
     4621 ctcgaaaact tatctcaaat tcaaaatagg gaatttggaa cacagaaata tgaacaatgg
     4681 attgttacag ttcagaaagc atgcatggtg ttccagatgc cagacaaaga tgaagagagc
     4741 aggatttgta aagccctgtt tttatacact tcacatttgc ggaaatataa tgatgccctc
     4801 attatcagtg agcatgcacg aatgaaagat gctctggatt acttgaaaga cttcttcagc
     4861 aatgtccgag cagcaggatt cgatgagatt gagcaagatc ttactcagag atttgaagaa
     4921 aagctgcagg aactagaaag tgtttccagg gaccccagca atgagaatcc taaacttgaa
     4981 gacctctgct tcatcttaca agaagagtac cacttaaacc cagagacaat aacaattctc
     5041 tttgtgaaaa ccagagcact tgtggacgct ttaaaaaatt ggattgaagg aaatcctaaa
     5101 ctcagttttc taaaacctgg catattgact ggacgtggca aaacaaatca gaacacagga
     5161 atgaccctcc cggcacagaa gtgtatattg gatgcattca aagccagtgg agatcacaat
     5221 attctgattg ccacctcagt tgctgatgaa ggcattgaca ttgcacagtg caatcttgtc
     5281 atcctttatg agtatgtggg caatgtcatc aaaatgatcc aaaccagagg cagaggaaga
     5341 gcaagaggta gcaagtgctt ccttctgact agtaatgctg gtgtaattga aaaagaacaa
     5401 ataaacatgt acaaagaaaa aatgatgaat gactctattt tacgccttca gacatgggac
     5461 gaagcagtat ttagggaaaa gattctgcat atacagactc atgaaaaatt catcagagat
     5521 agtcaagaaa aaccaaaacc tgtacctgat aaggaaaata aaaaactgct ctgcagaaag
     5581 tgcaaagcct tggcatgtta cacagctgac gtaagagtga tagaggaatg ccattacact
     5641 gtgcttggag atgcttttaa ggaatgcttt gtgagtagac cacatcccaa gccaaagcag
     5701 ttttcaagtt ttgaaaaaag agcaaagata ttctgtgccc gacagaactg cagccatgac
     5761 tggggaatcc atgtgaagta caagacattt gagattccag ttataaaaat tgaaagtttt
     5821 gtggtggagg atattgcaac tggagttcag acactgtact cgaagtggaa ggactttcat
     5881 tttgagaaga taccatttga tccagcagaa atgtccaaac ctgcaggtcg agctagcggc
     5941 agcggcgcca ccaacttcag cctgctgaag caggccggcg acgtggagga gaaccccggc
     6001 cccatggtgt ctaagggcga agagctgttc accggggtgg tgcccatcct ggtcgagctg
     6061 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6121 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6181 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6241 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6301 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6361 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6421 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     6481 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6541 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6601 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     6661 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     6721 taaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6901 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6961 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7021 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7081 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     7141 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7201 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7261 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7321 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7381 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     7441 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7501 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7561 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7621 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     7681 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7861 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7921 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7981 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     8041 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     8101 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     8161 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     8221 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8281 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8401 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8521 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8581 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8641 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8701 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8761 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8821 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8881 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8941 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9001 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     9061 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9121 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9181 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9241 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9301 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9361 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9421 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9481 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9541 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9601 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9661 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9721 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9781 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9841 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9901 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9961 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10021 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10081 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10141 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10201 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10261 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10381 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10441 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10501 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10561 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10621 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    10681 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10741 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10801 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
    10861 aacaaaagct ggagctgcaa gcttaa
//
