LOCUS       sequence-330941-       13134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression construct encoding a TagBFP in frame with a 
            self-cleaving wild-type RLR sensor RIG-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A RIGI WT
FEATURES             Location/Qualifiers
     source          1..13134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          63..128
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             258..278
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    703..837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(740..759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     794..813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1005..1638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1685..1810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2303..2536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2721..2765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3058..3175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3315..3722
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3741..4439
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4449..4505
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7558..7738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9266..9285)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9499..9518)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9618..9640
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9678..9696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9764..9868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9869..9937,9938..10729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10087..10106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10900..11488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11389..11408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11642..11659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11729..11749)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11734..12063
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11914..12049
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
       61 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
      121 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
      181 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
      241 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
      301 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
      361 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
      421 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
      481 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
      541 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
      601 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      661 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      721 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      781 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
      841 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
      901 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
      961 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     1021 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     1081 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     1141 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     1201 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     1261 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     1321 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     1381 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     1441 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1501 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1561 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1621 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1681 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1741 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1801 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1861 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1921 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1981 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     2041 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     2101 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     2161 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2221 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2281 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2341 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2401 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2461 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2521 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2581 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2641 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2701 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2761 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2821 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2881 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2941 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     3001 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt
     3061 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3121 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3181 ttattacagg gacagcagag atccactttg gctgatacgc gtctgcagcc ccgataaaat
     3241 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     3301 gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa ccaagaatag
     3361 agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa acaggatatc
     3421 tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc gcagtttcgg
     3481 ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc agcagtttct
     3541 taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct gcgccttatt
     3601 tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt cccgagctct
     3661 ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact gagtcgcccg
     3721 ggccgcgggc tagcgccacc atgagcgagc tgattaagga gaacatgcac atgaagctgt
     3781 acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc gaaggcaagc
     3841 cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg
     3901 ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc aaccacaccc
     3961 agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg gagagagtca
     4021 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     4081 gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc cctgtgatgc
     4141 agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct gacggcggcc
     4201 tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg
     4261 ccaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct ggcgtctact
     4321 atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac gtcgagcagc
     4381 acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac aagcttaatg
     4441 gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg
     4501 gacctggatc catgaccacc gagcagcgac gcagcctgca agccttccag gattatatcc
     4561 ggaagaccct ggaccctacc tacatcctga gctacatggc cccctggttt agggaggaag
     4621 aggtgcagta tattcaggct gagaaaaaca acaagggccc aatggaggct gccacacttt
     4681 ttctcaagtt cctgttggag ctccaggagg aaggctggtt ccgtggcttt ttggatgccc
     4741 tagaccatgc aggttattct ggactttatg aagccattga aagttgggat ttcaaaaaaa
     4801 ttgaaaagtt ggaggagtat agattacttt taaaacgttt acaaccagaa tttaaaacca
     4861 gaattatccc aaccgatatc atttctgatc tgtctgaatg tttaattaat caggaatgtg
     4921 aagaaattct acagatttgc tctactaagg ggatgatggc aggtgcagag aaattggtgg
     4981 aatgccttct cagatcagac aaggaaaact ggcccaaaac tttgaaactt gctttggaga
     5041 aagaaaggaa caagttcagt gaactgtgga ttgtagagaa aggtataaaa gatgttgaaa
     5101 cagaagatct tgaggataag atggaaactt ctgacataca gattttctac caagaagatc
     5161 cagaatgcca gaatcttagt gagaattcat gtccaccttc agaagtgtct gatacaaact
     5221 tgtacagccc atttaaacca agaaattacc aattagagct tgctttgcct gctatgaaag
     5281 gaaaaaacac aataatatgt gctcctacag gttgtggaaa aacctttgtt tcactgctta
     5341 tatgtgaaca tcatcttaaa aaattcccac aaggacaaaa ggggaaagtt gtcttttttg
     5401 cgaatcagat cccagtgtat gaacagcaga aatctgtatt ctcaaaatac tttgaaagac
     5461 atgggtatag agttacaggc atttctggag caacagctga gaatgtccca gtggaacaga
     5521 ttgttgagaa caatgacatc atcattttaa ctccacagat tcttgtgaac aaccttaaaa
     5581 agggaacgat tccatcacta tccatcttta ctttgatgat atttgatgaa tgccacaaca
     5641 ctagtaaaca acacccgtac aatatgatca tgtttaatta tctagatcag aaacttggag
     5701 gatcttcagg cccactgccc caggtcattg ggctgactgc ctcggttggt gttggggatg
     5761 ccaaaaacac agatgaagcc ttggattata tctgcaagct gtgtgcttct cttgatgcgt
     5821 cagtgatagc aacagtcaaa cacaatctgg aggaactgga gcaagttgtt tataagcccc
     5881 agaagttttt caggaaagtg gaatcacgga ttagcgacaa atttaaatac atcatagctc
     5941 agctgatgag ggacacagag agtctggcaa agagaatctg caaagacctc gaaaacttat
     6001 ctcaaattca aaatagggaa tttggaacac agaaatatga acaatggatt gttacagttc
     6061 agaaagcatg catggtgttc cagatgccag acaaagatga agagagcagg atttgtaaag
     6121 ccctgttttt atacacttca catttgcgga aatataatga tgccctcatt atcagtgagc
     6181 atgcacgaat gaaagatgct ctggattact tgaaagactt cttcagcaat gtccgagcag
     6241 caggattcga tgagattgag caagatctta ctcagagatt tgaagaaaag ctgcaggaac
     6301 tagaaagtgt ttccagggac cccagcaatg agaatcctaa acttgaagac ctctgcttca
     6361 tcttacaaga agagtaccac ttaaacccag agacaataac aattctcttt gtgaaaacca
     6421 gagcacttgt ggacgcttta aaaaattgga ttgaaggaaa tcctaaactc agttttctaa
     6481 aacctggcat attgactgga cgtggcaaaa caaatcagaa cacaggaatg accctcccgg
     6541 cacagaagtg tatattggat gcattcaaag ccagtggaga tcacaatatt ctgattgcca
     6601 cctcagttgc tgatgaaggc attgacattg cacagtgcaa tcttgtcatc ctttatgagt
     6661 atgtgggcaa tgtcatcaaa atgatccaaa ccagaggcag aggaagagca agaggtagca
     6721 agtgcttcct tctgactagt aatgctggtg taattgaaaa agaacaaata aacatgtaca
     6781 aagaaaaaat gatgaatgac tctattttac gccttcagac atgggacgaa gcagtattta
     6841 gggaaaagat tctgcatata cagactcatg aaaaattcat cagagatagt caagaaaaac
     6901 caaaacctgt acctgataag gaaaataaaa aactgctctg cagaaagtgc aaagccttgg
     6961 catgttacac agctgacgta agagtgatag aggaatgcca ttacactgtg cttggagatg
     7021 cttttaagga atgctttgtg agtagaccac atcccaagcc aaagcagttt tcaagttttg
     7081 aaaaaagagc aaagatattc tgtgcccgac agaactgcag ccatgactgg ggaatccatg
     7141 tgaagtacaa gacatttgag attccagtta taaaaattga aagttttgtg gtggaggata
     7201 ttgcaactgg agttcagaca ctgtactcga agtggaagga ctttcatttt gagaagatac
     7261 catttgatcc agcagaaatg tccaaatgac tcgagaccta gaaaaacatg gagcaatcac
     7321 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7381 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     7441 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     7501 acgaagacaa aatcgtcgag agatgctgca tataagcagc tgctttttgc ttgtactggg
     7561 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7621 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7681 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7741 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     7801 agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac
     7861 aggcaaagct gcagaagtac ttggaagaag ccaccagaga tactcacgat tctgcacata
     7921 cctggctaat cccagatcct aaggattaca ttaagtttac taacatttat ataatgattt
     7981 atagtttaaa gtataaactt atctaattta ctattctgac agatattaat taatcctcaa
     8041 atatcataag agatgattac tattatcccc atttaacaca agaggaaact gagagggaaa
     8101 gatgttgaag taattttccc acaattacag catccgttag ttacgactct atgatcttct
     8161 gacacaaatt ccatttactc ctcaccctat gactcagtcg aatatatcaa agttatggac
     8221 attatgctaa gtaacaaatt acccttttat atagtaaata ctgagtagat tgagagaaga
     8281 aattgtttgc aaacctgaat agcttcaaga agaagagaag tgaggataag aataacagtt
     8341 gtcatttaac aagttttaac aagtaacttg gttagaaagg gattcaaatg cataaagcaa
     8401 gggataaatt tttctggcaa caagactata caatataacc ttaaatatga cttcaaataa
     8461 ttgttggaac ttgataaaac taattaaata ttattgaaga ttatcaatat tataaatgta
     8521 atttactttt aaaaagggaa catagaaatg tgtatcatta gagtagaaaa caatccttat
     8581 tatcacaatt tgtcaaaaca agtttgttat taacacaagt agaatactgc attcaattaa
     8641 gttgactgca gattttgtgt tttgttaaaa ttagaaagag ataacaacaa tttgaattat
     8701 tgaaagtaac atgtaaatag ttctacatac gttcttttga catcttgttc aatcattgat
     8761 cgaagttctt tatcttggaa gaatttgttc caaagactct gaaataagga aaacaatcta
     8821 ttatatagtc tcacaccttt gttttacttt tagtgatttc aatttaataa tgtaaatggt
     8881 taaaatttat tcttctctga gatcatttca cattgcagat agaaaacctg agactggggt
     8941 aatttttatt aaaatctaat ttaatctcag aaacacatct ttattctaac atcaattttt
     9001 ccagtttgat attatcatat aaagtcagcc ttcctcatct gcaggttcca caacaaaaat
     9061 ccaaccaact gtggatcaaa aatattggga aaaaattaaa aatagcaata caacaataaa
     9121 aaaatacaaa tcagaaaaac agcacagtat aacaacttta tttagcattt acaatctatt
     9181 aggtattata agtaatctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc
     9241 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     9301 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     9361 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
     9421 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
     9481 cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     9541 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9601 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9661 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9721 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
    10021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
    10081 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
    10141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10381 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    10441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    11041 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    11101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    11161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    11341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    11401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    11461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    11521 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11581 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11641 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11701 gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
    11761 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
    11821 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    11881 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    11941 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    12001 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
    12061 aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc
    12121 gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc
    12181 gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga
    12241 taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca
    12301 gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat
    12361 tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt
    12421 catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg
    12481 ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc
    12541 ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc
    12601 gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc
    12661 tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa
    12721 tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata
    12781 gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct
    12841 gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg
    12901 cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat
    12961 gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca
    13021 ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat
    13081 tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg aagg
//
