LOCUS       sequence-330964-        8188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression of omega-dCas9 and sgRNA in E. coli: 
            lacUV2-omega-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and 
            UV5-BsaI-sgRNA (BsaI sites in place of the spacer).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Goldberg GW, Spencer JM, Giganti DO, Camellato BR, Agmon N, Ichikawa
            DM, Boeke JD, Noyes MB
  TITLE     Engineered dual selection for directed evolution of SpCas9 PAM 
            specificity.
  JOURNAL   Nat Commun. 2021 Jan 13;12(1):349. doi: 10.1038/s41467-020-20650-x.
  PUBMED    33441553
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-01-13"; volume: "12(1):349. doi"; pages: " 
            10.1038/s41467-020-20650-x"
COMMENT     Sequence Label: pGG369
FEATURES             Location/Qualifiers
     source          1..8188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..270
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             280..303
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(340..366,367..369,370..2856,2857..2859,2860..4443)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4462..4482
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4483..4572
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             4585..4611
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     misc_RNA        4704..4779
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(4791..4815)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      4945..5400
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5032..5051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5242..5263
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5426..5530
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5531..5599,5600..6391)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5749..5768)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6562..7150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7051..7070
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7304..7321
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7336..7476
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7402..7421)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7562..7584
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7578..7769)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    7934..7950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7939..7961
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
       61 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggaccc gctcgtaccg
      121 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
      181 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
      241 caagccgtta ccgctattgc tgaaggtcgt gccgcggccg actacaagga tgacgacgac
      301 aagttccgga ctggttccaa gacacccccc cacgggacca tggataagaa atactcaata
      361 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
      421 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      481 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
      541 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      601 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      661 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      721 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      781 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      841 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
      901 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
      961 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     1021 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     1081 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     1141 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     1201 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     1261 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     1321 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     1381 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagcg
     1441 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     1501 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     1561 ggctctattc cccatcaaat tcacttgggt gagctgcacg ctattttgag aagacaagaa
     1621 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcgc
     1681 atcccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     1741 aagtctgaag aaacaattac cccgtggaat tttgaagaag ttgtcgataa aggtgcttca
     1801 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     1861 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     1921 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     1981 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     2041 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     2101 aatgcttcat tagggacata ccatgatttg ctaaaaatta ttaaagataa agattttttg
     2161 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     2221 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     2281 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     2341 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     2401 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     2461 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     2521 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     2581 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     2641 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     2701 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     2761 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     2821 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     2881 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     2941 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     3001 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     3061 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     3121 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     3181 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     3241 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     3301 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     3361 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     3421 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     3481 acagaaatta cacttgcaaa tggagagatt cgcaagcggc cgctaatcga aactaatggg
     3541 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     3601 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agaccggtgg attctccaag
     3661 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggac
     3721 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     3781 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     3841 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     3901 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     3961 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     4021 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     4081 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     4141 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     4201 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     4261 aatattattc atttatttac gttgacgaat cttggagcgc ccgctgcgtt caagtacttc
     4321 gacaccacga tcgatcgtaa acgatatacg tctacaaaag aagttctaga tgccactctt
     4381 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     4441 gacggcggtg gatccggcgg tccgaagaag aagagaaagg tgtaccccta cgatgttcct
     4501 gattatgcgg gctatcctta tgacgtacca gactatgctg gtagctaccc gtatgatgta
     4561 cccgattacg ctggctccgc tgcaccagcc gccaagaaga agaagctgga ttttgaaggt
     4621 acctaatagt gagtcgagct gcccgggttg acacgcaagc tcagtcctag gtataatact
     4681 agtcgagacc aatctcggtc tcagttttag agctagaaat agcaagttaa aataaggcta
     4741 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctac acccagcttt
     4801 cttgtacaaa gttggcatta actcgaggct agagactaga aaaaggccga caagtcccgc
     4861 tccgctgaag atcctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4921 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5161 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5221 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5281 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5341 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5401 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5461 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5521 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5581 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5641 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5701 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5761 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5821 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5881 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5941 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6001 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6061 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6121 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6181 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6241 gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6301 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6361 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6421 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6481 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6541 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6601 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6661 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6721 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6781 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6841 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6901 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6961 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7021 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7081 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7141 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7201 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7261 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7321 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7381 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     7441 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7501 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7561 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     7621 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     7681 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     7741 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     7801 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     7861 gaacatgccc ggttactgga acgggaattc cgggctttac actttatgct tccggctcgc
     7921 attatgtgtc gacttgtgag cggataacaa tttcacacag gaaacagcta tgcctcaaca
     7981 gcagcaaatg caacctccca attcaagtgc ggacaacaac cctttgcaac agcaatcatc
     8041 acaaaatacc gtaccaaacg tcctcaacca aattaaccaa atcttttctc cagaggagca
     8101 acgcagctta ttacaagaag ccatcgaaac ctgcaagaat tttgaaaaaa cacaattgta
     8161 agtctcggct ccaagaagga gatatact
//
