LOCUS       sequence-330969-        9342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QuasAr2-mOrange2 voltage sensor (plasma membrane).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Werley CA, Boccardo S, Rigamonti A, Hansson EM, Cohen AE
  TITLE     Multiplexed Optical Sensors in Arrayed Islands of Cells for 
            multimodal recordings of cellular physiology.
  JOURNAL   Nat Commun. 2020 Aug 4;11(1):3881. doi: 10.1038/s41467-020-17607-5.
  PUBMED    32753572
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-08-4"; volume: "11(1):3881. doi"; pages: " 
            10.1038/s41467-020-17607-5"
COMMENT     Sequence Label: pMOS009: QuasAr2-mOrange2 voltage sensor (plas...
FEATURES             Location/Qualifiers
     source          1..9342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1483..1983
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1902..1920
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1995..2390
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2684..2987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2988..3191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3142..3162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3214..3238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3273..4031
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             4038..4745
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     protein_bind    complement(4859..4883)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4885..4926
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4968..5556
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5021..5041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5439..5450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5628..5861
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5939..6060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5976..5995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6030..6049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6100..6235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6255..6274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6256..6274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6284..6301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6284..6300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6293..6315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6442..6897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6529..6548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6739..6760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6923..7027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7028..7096,7097..7888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7246..7265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8059..8647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8548..8567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8801..8818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8935..8956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8971..8976,8977..8994,8995..9001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9009..9025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9014..9036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9033..9049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9033..9049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9068..9088
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9070..9088
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9116..9342
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
     2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
     2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctgg
     3241 cgccggaacc aattcagtcg actggatcca ccatggtaag tatcgctctg caggctggtt
     3301 acgacctact gggtgacggc agacctgaaa ccctgtggct gggcatcggc actctgctga
     3361 tgctgattgg aaccttctac tttctggtcc gcggatgggg agtcaccgat aaggatgccc
     3421 gggaatatta cgctgtgact atcctggtgt ccggaatcgc atccgccgca tatctgtcta
     3481 tgttctttgg tatcgggctt actgaggtgt ccgtcggggg cgaaatgttg gatatctatt
     3541 atgccaggta cgcccagtgg ctgtttacca ccccacttct gctgctgcac ctggcccttc
     3601 tcgctaaggt ggatcgggtg accatcggca ccctggtggg tgtggacgcc ctgatgatcg
     3661 tcactggcct catcggagcc ttgagccaca cggccatagc cagatacagt tggtggttgt
     3721 tctctacaat ttgcatgata gtggtgctct atgttctggc tacatccctg cgatctgctg
     3781 caaaggagcg gggccccgag gtggcatcta cctttaacac cctgacagct ctggtcttgg
     3841 tgctgtggac cgcttaccct atcctgtgga tcataggcac tgagggcgct ggcgtggtgg
     3901 gcctgggcat cgaaactctg ctgtttatgg tgttggacgt gactgccaag gtcggctttg
     3961 gctttatcct gttgagatcc cgggctattc tgggcgacac cgaggcacca gaacccagtg
     4021 ccggtgccga tacgttgatg gtgagcaagg gcgaggagta taacatggcc atcatcaagg
     4081 agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg
     4141 agggcgaggg cgagggccgc ccctacgagg gctttcagac cgctaagctg aaggtgacca
     4201 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcatttcacc tacggctcca
     4261 aggcctacgt gaagcacccc gccgacatcc ccgactactt caagctgtcc ttccccgagg
     4321 gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg cgtggtgacc gtgacccagg
     4381 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     4441 cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     4501 tgtaccccga ggacggtgcc ctgaagggca agatcaagat gaggctgaag ctgaaggacg
     4561 gcggccacta cacctccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     4621 ccggcgccta catcgtcgac atcaagttgg acatcacctc ccacaacgag gactacacca
     4681 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     4741 acaagagtag aatcacaagc gaaggcgagt acatccccct ggatcaaata gacataaatg
     4801 taggtggatt ttgttatgag aatgaataag aattcgcggc cgcactcgag atatctagac
     4861 ccagctttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
     4921 tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
     4981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5341 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5521 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     5581 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5641 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5821 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     5881 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6061 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6121 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6181 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6241 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6421 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6781 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6901 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6961 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7021 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7081 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7141 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7201 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7261 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7321 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7381 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7441 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7501 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7561 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7621 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7681 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7741 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7801 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7861 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7921 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7981 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8161 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8701 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8761 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8821 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8881 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8941 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9001 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9061 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     9121 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     9181 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     9241 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     9301 tgcagagata ttgtatttaa gtgcctagct cgatacataa ac
//
