LOCUS       sequence-330972-       13289 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of PmcCAST proteins (except TniQ). Two BsaI 
            sites for spacer cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13289)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 13289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS22: pHelper(PmcCAST)_entry_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..13289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        270..288
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    289..313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      4212..4259
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4405..4423
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4424..4448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        9772..9790
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    9791..9815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9924..9968
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      10020..10067
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10188..10979)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(10980..11071)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11119..11857)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(12067..13149)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     13111..13130
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(13150..13227)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(13273..13289,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtgagtc gtggctttat gccgttaggc gttgctcaaa gagacctctg
      121 gtctcagtga gtcgtggctt tatgccgtta ggcgttgctc aagatccgaa ttcgagctcg
      181 gcgcgcctgc aggtcgacaa gcttgcggcc gcataatgct taagtcgaac agaaagtaat
      241 cgtattgtac acggccgcat aatcgaaatt aatacgactc actatagggg aattgtgagc
      301 ggataacaat tccccatctt agtatattag ttaagtataa gaaggagata tacatatgac
      361 agctatatta attcaaagtg aggaatatgt agacttaaca tttaagctaa gaggcgcacc
      421 gattcctctt gacaacggtt atcttaccta cgctgcctta tcccgtattt gtcccccact
      481 acatgaacta aagtctattg ggattcatcc gattgctgga ataccgacca ggaacaattt
      541 gcttgagcta actgctcaat ctcgtctaaa aattcggatt tatcatcagc aaattccttt
      601 aatttatcct tatttagcgg gtcaagcttt tcatataggt caaaattttt atcaactaga
      661 tatccccgac tacaaaccgt taatttcttc agaaagtgtc tattccagat tagtgataat
      721 taaagggttc caagactcta ctaactttat tgaagctgtg caacggcaaa tggataattt
      781 aggaatacaa ggaaaaattg aacttcttac ccgacaagat ggaacacctc aacgaagaca
      841 attaaccatc aataaagaag ggaaacagtt taaagttaga ggatttggcg taaaaatcag
      901 tgaactcaat cctgaagatt ccttaacctt gcaagaacag ggaattggcg ggaaacgaaa
      961 gatgatgtgt ggaatatttg tcccagcgac tcgcagcaag gaagaagagg aaacctgatg
     1021 atagtctcta cccaacccaa aatttcccta tctctccatg ctgcggatac gacaataatg
     1081 caccgcgtcg gaatgaccgg actttacatg actttaaagc gattggaaaa gcaatatccc
     1141 ttatctcgtc aacggggagg acatatatca tggtttttga ctgctgatac tattgaatta
     1201 ttctgggaag gaagtgattt catcgcctta tcttggttaa ttaatgagtc ttttcaatta
     1261 gatgatacag gtttaattca tttagtagga ttagacaatg atcgaataga tttaaggcag
     1321 aaaatccata ttcatgaggg aatatgcggt gtttttctac gtcttaataa gttttatcaa
     1381 gcaggagaga taattaacac tgaactaagg tttgaagaaa aacaagtcga gtatcaatat
     1441 aaatccctaa cttggtacgc acatcaaacc tttgcagaga agttatgcga ggcagatacc
     1501 caacaactca gacatgatta cattcaaata accagttggt tgtatttagg gggaattgtt
     1561 cgccatgctc ggacgcagaa tactacaaaa ctagaagaga aacctgaata cgctttagca
     1621 ttattatttg taccagtagt ttgtcattat tgcttacttc atataccatc agaagactta
     1681 aaagaaagga aaccccatcg ctacttggtg gtgattccag aaataaagga ttttgaagac
     1741 gcttctcaaa ggagatggcg attacagcaa ttagaaacta aacagtttca tgttagtagc
     1801 cttggggaag caggattact ttattacagc ttagacgata ttcagcctga agtggcctac
     1861 tatcaagctt gtcaagtttg gttatatgaa aaaacaaata aagcttctcg tcaacggaca
     1921 ttgatgagta tagaagaaat taaaattgat aagaatattt taatcactta tcaacaggtg
     1981 caaaaatact ttaaaactaa ttatcagata attaaatata agcaaatttt tattaaggtc
     2041 aatccgattc gctccctgat tgctgataat ttagtgaaag gaatccattg gtggtctaat
     2101 ttttgggaaa aattggtgat agaggattca aaagaatatt tatttaacca gttattttcc
     2161 aatcgagaag gattcatcat aatggcagag aatagtgaag aagacaagca gtatcttatt
     2221 tttattaagg tatttcagca agctatgaag ggcaactttg ctaaaattta tgccaaaaca
     2281 gaggaaggta aagatcctcc aattaagaag aaagttgaac gattaagggc agagctaaac
     2341 tattgttatg atgaattgtc gtttaaggaa tatttgtctg actttttagt aagaggggga
     2401 ttaaataagt attttaatga gcatcaggaa gagattgcac tactaattaa aaaatcacct
     2461 tggcaagaaa taagaatttg gtcattgtta gcgatcgcca gttataaacc caaagataaa
     2521 ctcactaata gggatgatag ttcattgtca aacaatcaaa aactagaaga agtgaatgat
     2581 gactcagaag aagaatgaca gtaatatccc aaattattat ctttacggaa cagtcctcac
     2641 tcgttatggt ttagcttcct taaatcatga cattagacga ggaaataaga ctattctgca
     2701 aaaaggctat tggaataatg gtaaaattca ttcttttgta ggttcaagtg caattcgttg
     2761 ggcgttacgt ttttatcttc aaaagcaagg ttatttagtt aatcgagtct gggatgagga
     2821 agaacatatt aatcggttaa caagtgaaga tttcgatcca gaaaaatttt atgatgatga
     2881 tatttttggg tttgctttat tagagtctgc tgaaacagag gaggacactt caacaacaaa
     2941 gagaaaaaag aaacaaacaa agaccagcac tcctaatcag cgaatgggtg ctttagggat
     3001 gaatatggct gtttcgctaa cgccttatga tggtgcagtt aagttagggg caaaaagtgg
     3061 cagagagaag gatagtacat ctctccattt tacagaatat catgcaactc gatatcaata
     3121 ttattttgga attgatgcca ctcatctcaa agatttttcg cggattttac ctatgataga
     3181 tgggattatg aatctgccca aggtaggagg gagtagtaat atttttaatt atcctttctg
     3241 tcccgatagt ttggtatttc aatggacaaa tcattttgct tcatatattt cctattgctt
     3301 tgaatattgc gatcctaaga gtaaggaagc aaaactttca caagagttta tagatgaagt
     3361 ggaatgcgga cagattgacc ctagtaaatt gtggattggg ggaacaattg ttaaggattt
     3421 acagcaattg gataattttg aaagttcccc tttgaacaag gcgcatattt atcgtaatcg
     3481 aaatgaaatg attgaagctt tgaaaacagt cattaaaaga gatttgggct tggaagaatc
     3541 aaaatgattg caccattaat tctctatttg gatgttccat ttacgacttt tcgggagtcc
     3601 catgcaaggg aaatgggaaa aacctatcct gttcctcctc cagcgacagt gtatgggatg
     3661 ttgttgtctt tggtaggaga aacgaatgtc tatcgccact gtggggtaga attggccatt
     3721 gcaatgttat ctagccccaa aaagtcgcga attttgcgtc aaatgagacg gtttaagaat
     3781 gctgatttta gccatccaga gaatgttatt ccctgttatc aagaaatttt gtctaatctg
     3841 aagtgtttga tttgggttcg ttctgacgag gaaaagatac aaccaagttt aagggagagg
     3901 atacagttag cgtttgacca tcctgagcta gtaagacggt ttggttgttt atttttggga
     3961 gaaagtgacc agttgattaa aacgatcaaa cttgcaaggg aagattatct agagggagtg
     4021 agacagtggg cgattagaga taatcgaggc aggttaactt taccctattg ggttgaccat
     4081 gtggggtcaa gaaatacccg atttttgagg tatcggattg aggaaatgga tagattatca
     4141 cctcctgatt tagcctggac aatggttcaa tctccgattt gacctaggct gctgccaccg
     4201 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     4261 tgaaacctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     4321 taaaccagca atagacataa gcggctattt aacgaccctc gggatctcga cgctctccct
     4381 tatgcgactc ctgcattagg aaattaatac gactcactat aggggaattg tgagcggata
     4441 acaattcccc tgtagaaata attttgttta actttaataa ggagatatac catgaaagag
     4501 caatttgaac aatgggttgc tcgccttcaa atatccgagg cagggcaaaa aattattgag
     4561 aaagttcgct catctgagcc atcccgtcgc gttggtggcg gtagcaagaa tgtttcggga
     4621 cgctacccta gtcgcaagat gggagtcacg attcaatttg agtctcatcg ggttgaactg
     4681 ccaattatct atcagttaga acatgataaa gatgttctag aattctacga tcaaccaccg
     4741 caaattaaac ttgactatca agcaagtaac ggacgtagat taggcgtttt acacacccca
     4801 gatttatttg tcatccgaac caattcggct ggttgggaag agtgtaaaac tgagcaggat
     4861 ttgaaaaaac tagcagagaa aaaccccaat cgttactttt actctgaaga aaataatcaa
     4921 tggcattcct tgccaggaga agtttatgct aaccagtttg ggctttatca ccgcattcat
     4981 tcagatcgcg aaatcaattg ggttctgcaa cgtaatttgc aatttttaga agattattat
     5041 cgctcagaat ctctagtagt ggaagaagcg atcgctcaat cattacttgc aattgtgtca
     5101 tctcaaccag gaatcacttt agcagaatta ctcaatcggt caacaggagc taaatctgac
     5161 gatatctaca ccttaattat tcaagagcaa atctatattg atttaaatgc tacaccacta
     5221 gctgaaccag aaagatgtct aatcttccgt gacgagcaaa ctgctagtgc ttatagatta
     5281 atggttgagc aaccaagtgt aagtcttccg gctatctctc ctgtagtcaa tattgttact
     5341 ggagcaactg ttagttggga cggcaaaggt ttaaatatca tccatgttgg agaaacagag
     5401 gttatacttg gcgcagaaaa taatcagtta attgaactca aaaaagctat atttgagaat
     5461 ctggttcgcc aaggaaaaat cacaagtctc caaacaccag aaaaaacagc tattagcaca
     5521 gaatcttggc agcgatttca tcaagctagt cctgaagacc aagcagaagc ccttgaacgc
     5581 tacaaaacta tcgaacctta cttaaacgga catcctccag aaaacgaaac aataccagcc
     5641 cgtaccattc gtcactggaa agctaaatat ttaattgcaa ttcaaaaata cggttgcggc
     5701 tacattgggc ttttgtccca tcgcagtgtc aaaggtaatc gacagcgcaa gctgcccgaa
     5761 gacaccttag caattatgga gaagtttatt ttagaagact atgaaactct taaacaaaaa
     5821 agaatgtggg aggttcacgc ggctttagtt cgagcttgcg aagaagctgg tgtaattgct
     5881 ccgagctata aagcatttac caaagaagta caacgccgga ctggttacga acagactaaa
     5941 aaacgccaag gtcgtcgtgc cgcttatcag catgaatcct tctattggga gcttgctata
     6001 actaccccaa gacacggcga tcgccctttt gaaattgggc atatagatca tactgagtta
     6061 gatgtagagc tagtttgctc tgatacaggt cgcaatcttg gcagaccttg ggcaacattt
     6121 ctcgtagatg cttattcacg gcgattgctg gcagtttacc taacgtttga ctcaccctca
     6181 tatcgttctt gcttaatggt gctaagaatt tgtgtcaagc gtcatggcag gcttccccaa
     6241 attgtagtgg tagataacgg tgcagagttt cacagcgttt actttgaaac actactagct
     6301 acttttgaat gtacaaaaaa gcaaagaccc ccagccaaag ctcgttttgg ttcggtttgc
     6361 gaacgcctat ttggaacttc caatactcaa tttgtccata acttacttgg taatacacaa
     6421 atcactcgta atgtccgcca agtcactaag tcagttaacc ctaaaaatct agctgtatgg
     6481 acacttggtt tactttatga atatctttgt gcttgggctt acgaagttta tgatacagac
     6541 gaacatccgg cacttttcca gagtccgagg gatgcgtttg ctgcgggtat ggcgattgga
     6601 ggcagtcggg ttcaccgcat gattccttac gatgaaaact ttcagattct taccctacct
     6661 acaacctcag agggaaaagc aaaagttcaa gttggacgcg gcataaaaat taactacata
     6721 tactactggt ctaatagttt ccgcgatcct caaattgaaa atacctctgt gcagataaga
     6781 tacgacccgt ttaatattgg catcgcttac gcctttgttc gaggacagtg ggtacagtgt
     6841 atctctcaat attatgcaga actccaaggt cgttcagaaa aagaactgaa gttagctagt
     6901 atcgagttac gcaaacacta ttcaaatcat acacaacaat ctaaagtttc ggctaagaat
     6961 ttagcagaat ttttagcatc ggtagaagct caagaagctc tattagaaca gcgttcctat
     7021 gatgcagaag ttaaggaagt ctttagggta atcgagggtg gaaaagcgac aacaagccgt
     7081 aacgaagaac caaaacttat tcaagttact tttgccaata ccgattttca agcggatgaa
     7141 gatgaagcga tcgtcccaga aacacttgta gtgtatgagg agttttaact atgacaaaat
     7201 ctactggatt tccgcttgaa ctcctcacac ggcctgctac agaaaggctt gcttactttg
     7261 aaaattacac cgtagcgcat cctcgactta aagaagtcta tgaaattctc atgcgaacga
     7321 ttgccgaacc agcaggagct tcatttatct ttgtttacgg tgctagtggt gttggtaaaa
     7381 caactttgcg actccgcgtt gagcagaaat taacagaact agcattacct aaattggaaa
     7441 gcgatcgcgc tcgtgttcct gttgttggta ttgaagctat agcgccagaa tcgagatact
     7501 tcaactggaa agaatactac acacgcgcct taattacttt ggaagaacca ttaatcgatc
     7561 acaagtttga ttacggtgtg cgtggtatct ctcgcgataa ttttggcaaa attaatgttg
     7621 agtcgaaagt agttgcacct gctctacgca gagctttaga aaatgctctc atccatcgtc
     7681 atccagacgt attttttgta gatgaagcac aacattttgg taaggtggca agcggataca
     7741 aactacaaga tcaattagac tgtctcaaat ctttagcaaa tatgacaggc atcttgcact
     7801 gtctattagg aacatacgaa ctacttacat tccgtaattt gagcggtcaa ctctcacgtc
     7861 gtagtgttga tattcatttc cgccgttatt gtgctgatag cccagaagat gtacaggctt
     7921 ttaagagtgt gttgttaact tttcaacagc atctgccact agcagaaact ccaaatttag
     7981 tcgatcattg ggaatatttt tacgaacgta ctttaggatg tattggcaca ctgaaagatt
     8041 ggctgaagcg tgttcttagt gatgctttgg atcgagaagc aacaacaatc actctcaaag
     8101 atttacaaaa acgtgctttg tcggttgctc agtgtcagaa aatgttcaag gaaattcaag
     8161 agggtgaacg acaattatct gaaacagaag cagatgtgca aaacttacgc tctgcattag
     8221 gacttggcgc aaaaccaatt gtgctacccg aagaaacccc aaaaactact cgtccgccag
     8281 gaaaggttgg caaacgcaaa cctaaaagag atcccatcgg agtgcagcaa gatgttagct
     8341 agtgtattag aaacatacga atcttggaat ttaaaaaagc cattaattcc acaacgtagt
     8401 cgcctctatc aacctcaacc tgtaggtatt gggactcctt acatagagag tttaacaggt
     8461 tatatcacac ggatagcaga gttgcatgga gttcttccag gagtcttgat gactagagaa
     8521 attgctccgc ttgtcaataa aatatatttc caaaatggag ctaatcgagg ttttcgtgaa
     8581 atattcaacc gtagtcaagc gttaaatggc atgggagaga tggcagcaga tttagttcaa
     8641 gtgttgcaaa aattgacgct tagagatgac ttgcgttttt taaccatgct gttttggtct
     8701 aacattttga ctccacgcaa tttatttcgt accagaaaag cttggtgtcc aatttgttat
     8761 caagagcggc atcaaaatgg gctggtagtc tatgagcaat tgctttggac aattaaccta
     8821 attacaattt gcccgcagca tcaaaaaccc ttggttgaac tttgccctca ctgtaatcat
     8881 gaatcacctt tgttaaactg gcgctcaaga cctggttact gttcaaagtg tggagagtgg
     8941 ttaggcgcaa atcaatgcct taagacattt acagatggtg aaggatcaat aaaattacag
     9001 ttggagtggc agtattggac tgccaatgtt gtaggagagc taattttagc ttctcaatgc
     9061 tttgaatctg ccccatcaaa agaaaatatt acaaaatcat taaatatagt tattgataaa
     9121 gttgcggaaa ataatgcagc tgcattttct cgtcttattg gagtaccaaa aaatagtttg
     9181 tggatgtggc agtctactaa aaccttgcca gagctaaata cacttctgaa aatttgttac
     9241 gaattagaga tatcgttggt agaatttctt actccaaaaa atctaattac taaatctttt
     9301 acaaaaatct cgcagaagca cctccaactt tctcgcacac caagggtatc tccaaaatct
     9361 ttcgaccaat atcaagtaaa agatgcttta ttagcaatac ttgcaggtaa tgaagaacca
     9421 ccaccaacaa tggaagaagt tggaaagcgt ttaggtcatc acaacagaac tatttctaga
     9481 cactttccag atttgtgtag tgctatttct gctaaatgtc gtaattacaa taaagcttgc
     9541 cgtctcaaaa gcatagaaaa actttgcagc gaagttagag agattgtcct tagccttaat
     9601 gctcagggtg tgtatcccac agaaggtcgt gtttgtgaat taatgccaaa tccggggtgt
     9661 tttagatata aacaagtccg cgctgctttt aatgatgcac gacgtgagtt tggtttgtaa
     9721 cttaagtcga acagaaagta atcgtattgt acacggccgc ataatcgaaa ttaatacgac
     9781 tcactatagg ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat
     9841 aagaaggaga tatacatatg gcagatctca attggatatc ggccggccac gcgatcgctg
     9901 acgtcggtac cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca
     9961 tggactcgtc tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac
    10021 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg
    10081 catttgagaa gcacacggtc acactgcttc cggtagtgcc ctgaaccgac gaccgggtca
    10141 tcgtggccgg atcttgcggc ccctcggctt gaacgaattg ttagacatta tttgccgact
    10201 accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc tgcgcgcgag
    10261 gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat gacgggctga
    10321 tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg cgcgattttg
    10381 ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg ctcatcgcca
    10441 gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc aaatagatcc
    10501 tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc aacgctatgt
    10561 tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg ctcgaagata
    10621 cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt agctggataa
    10681 cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg gagaatctcg
    10741 ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg ccgcgttgtt
    10801 tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg cttcaggccg
    10861 ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag atggcgctcg
    10921 atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc ttccctcata
    10981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    11041 atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg ctacgctccg
    11101 ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga ttccgtggat
    11161 aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg tttttccata
    11221 ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca tctgtgggag
    11281 ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac ttaaagatcc
    11341 ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct gccgtttacc
    11401 ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat agctcacaca
    11461 ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc
    11521 cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa
    11581 gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag aggatttgtt
    11641 tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac tggaaggaca
    11701 gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc cccaactgac
    11761 ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc aaaagattac
    11821 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac cgctctagat
    11881 ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt
    11941 gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
    12001 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
    12061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    12121 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
    12181 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
    12241 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
    12301 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt ccgcaccaac
    12361 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
    12421 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
    12481 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
    12541 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
    12601 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
    12661 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
    12721 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
    12781 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
    12841 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
    12901 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
    12961 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
    13021 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
    13081 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
    13141 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
    13201 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
    13261 gcattaggaa attaatacga ctcactata
//
