LOCUS       sequence-330973-       15689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins. Two BsaI sites for spacer 
            cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15689)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 15689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS4: pHelper(AvCAST)_entry
FEATURES             Location/Qualifiers
     source          1..15689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        325..343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    344..368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      4347..4394
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4543..4561
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4562..4586
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10366..10384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    10385..10409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      12398..12445
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12647..13438)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13439..13530)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13578..14316)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(14526..15608)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15570..15589
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15609..15686)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg
      121 agatatacca tggtgcttta acattagatg tcgttaggcg ttgagcagag acctctggtc
      181 tcgtgcttta acattagatg tcgttaggcg ttgagcagat ccgaattcga gctcggcgcg
      241 cctgcaggtc gacaagcttg cggccgcata atgcttaagt cgaacagaaa gtaatcgtat
      301 tgtacacggc cgcataatcg aaattaatac gactcactat aggggaattg tgagcggata
      361 acaattcccc atcttagtat attagttaag tataagaagg agatatacat atgattattg
      421 atagtaactt agttacgcag cttccctcag aatcatcttc aaagccaaga gagcttttaa
      481 tgtctcagtt tccatatatg gatttaactt ttagccttac gggtgagaca ttacctttcg
      541 accacggcta tgaattattt tctgcgatcg cccactttga acccaaacta catacattag
      601 acacgttggg aattcagacc attgcaggta ttcccaagga tggtgtaatt aatctgaccc
      661 aaaattctag gcttcgtatt cggataccag taaatcaggt acgtttggtt tatccattgg
      721 cgggtaagtc actgagaatt ggcaagcata caattcgttt gggtattcct gacatctgtt
      781 tgcttcaacc tgcggagaaa ctgcgatcgc gcattgtggt aataagaggc tatgaagaac
      841 ctgaaagttt tttagtcgta gcgcagcgtc agctagaaca actgggtatc caagcaattg
      901 ctagcattcc caccaaagcc aatggcaaac caatacgtag gactatcaaa attaagcgat
      961 ttacagtcgt gggttttgga ttagaagtta taaatttaag cgatgaagac tcactgaggt
     1021 tgcaaataca tggtgtagga ggaaagcaaa agatgggttg tggactcttc atgcctatta
     1081 aggaaaggca atagcaatta ctcgaaagca gatgatgaag agagcagata accccaaaat
     1141 tcagcttaat ctcggcgatc ctagtatgac tttattgcat cgtgctgggg ttgcaggact
     1201 ttggatgacg ctcaaaaaac tggaaaaaat ctatccaaca cctgctgaac gtattggtaa
     1261 tttaacttgg ttgctaaccc ctcactatat caatcttgat tgggaaggac aggatttcac
     1321 agttttagac tggttgctga agcaatcatt tcaaatcagc gatgagggtt taatttccct
     1381 gacagggtta gattctcaaa acatggatat tcaaaatcaa ataattatcc atcaaggtat
     1441 cacagccact tttacacaac ataataaatt ttttaagtct gcgggtcaac aatctaagca
     1501 actaattatt gatggtattc aggttagggt tgattataag aaagctgctt cctatgctca
     1561 tcaacatttt gctaaacatt tatgtgacca gtacggacaa ttgctaaaag agccaattgg
     1621 tatcagggga tggctctatc caggagcagt agttcgccat tatgctttta aagaacaaac
     1681 agcatttgaa gaaaaagcag aatatgctct agcgttgttg ttcgctccta tggcttgtca
     1741 atattttgtg ctgcgatcgc acaccgaacc atcacataca aactatgttt tggttattcc
     1801 tgaagtaatt gatttagaac tttatgcaca ttttttgtgg tgtttgggta acttagatta
     1861 taaacatttt cacgtatcta gcttaggaga tgctggatta aaatttctta attataaaac
     1921 aacacaaatg cctacttcca attttttaaa acgatgtcaa ataatctttt ttagtacaaa
     1981 agcttggtca gaacagcaaa aaactcgtgc agaaatagca atcatagaat taaattcttc
     2041 agttaattat ctttataagc ttagttgtat ttgttttcca gaatgtcaaa ttgttttata
     2101 taaaaatcaa cattatttgc ttccaagttt aataaaagga ataatcgcca ataatctagc
     2161 aatgggtcgg tcttggtggg ctaattggat ttcatttatt aaaaataaaa aatcatttaa
     2221 acaatttact gatgagtata aaggaattaa taagatgatt caggactcag aatgggatat
     2281 agaagctcaa aagctattta ttcaagcttg tcatgaagca ttaagaaaaa tatatgccaa
     2341 aatttatagc agaacaaaag aaggtgaata cgctcaaata gaacgagaaa atacacgcat
     2401 tttatctcag ttaaaacgct gcaccaatac tgacaatttc cgaaaattta ttgcagagtt
     2461 ttggggaagg gcagggcaaa tagcaattct ggaagaacat tggatggaat tacttccatt
     2521 gactacaggg ataggcaatt ggaaagtggc cagggactta acttttattg caatggctag
     2581 ctacccaaag aataaaataa cagaggtaga ggtaaaacca tcacaaatat cggagctaaa
     2641 tactgaataa acttcatatt ttttgatttg attaagaaca aaatattaat tgtttaggta
     2701 atggtggaat gtttcatctg tttggtaata ttttgactag ttatggaacc gccgctaata
     2761 atcgtggtga aaatgaaggt aataccacta cgttacaaaa attaatttgg aaaggtgaag
     2821 ttcactctac tgtttcatct gaggcaattc gttgggcatt gcgatattat tggcaaaatg
     2881 ctggctatcc tgtgaataga cgttgggatg aaaatgctca accagtaccc gatcacatct
     2941 tgcaagatcc taattttgat gatattcgct tcattgatga tgatgtcttg ggatttatgc
     3001 aagcggaagc agcaaaagtt gaagcagcag ttgaatccga agttgaaatc cagaactcaa
     3061 ctcaagatga ggacaaaaaa ctttctcaag gtaaggagaa agaaactgca aaacgtaagc
     3121 gtaaggaaaa acctaaaggg aaaataacta ctagacgagg tgtattagaa gtcactcgtg
     3181 cagtttcaac aattccttat gctggtgatc ttactttcaa tgctatgagt ggaaagaaag
     3241 gacgaacatc tctttattct acagaagttc atgctactcg atatcaatat ggcttcgcat
     3301 taacacccaa tagcctcaaa gataaatctc gcattaatgc tgtcctcgat agcttaattt
     3361 ctattggaga agtggcagga aatcacgctc gttttctcta tgatttttct cctgatagca
     3421 tcatattacg gtggacacac gatttctcac ctcggttact ttactgtttt gaagaagatg
     3481 aacttagaaa tatatcaaca ccggatttag taaggcgggt agaatctgga gatatagacc
     3541 ctaaagaatt atggatagga ggggcaattt ctagcactct ggaagattta ggggcaaatg
     3601 tgtttcccgg agtgaagaca acagttgaag cattaaaaca ggtgattatt gaagatttgc
     3661 agttgtcgtt aaggagttca tgatatgacg acaattgcct taaaagtaga agttcccata
     3721 gcttgttttc ggcagtctcg tgctagggaa tatggcgaaa cttaccctgt accaccacca
     3781 tcgacagtat atggaatgtt actatctctt gttggagaag tggatagata caaacactgc
     3841 ggtgtgaagc tggcgatcgc acttctatct gaaccagaaa agtcaactgt tattcgcacc
     3901 tttcaccgat ttaagacgaa gaatatccat gactctaaaa ataataaacc tgattatcag
     3961 gagttattaa ctaatataga atttatagtt tgggtggatg ctggagtaga taaagctaaa
     4021 ccaaatttag tggaacgttt agcagaggca ttgactaacc cggcttctat taataggttt
     4081 ggtggattat gtttaggtga aagtcgggat ttagtgaatg atgttaccct tttacctaaa
     4141 aattactatg cagaatcaat gcgatggtta attcgagatg aatatgggtt actaacttta
     4201 ccttactggg tagaccatgt aggttcgcgg ggaacgcgat ggctacgtta cgagattcag
     4261 aaatgtccag tctttcaacc acctgaatta tcttggactt cagttcaaag taattagcct
     4321 aggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     4381 tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg
     4441 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg
     4501 gatctcgacg ctctccctta tgcgactcct gcattaggaa attaatacga ctcactatag
     4561 gggaattgtg agcggataac aattcccctg tagaaataat tttgtttaac tttaataagg
     4621 agatatacca tggttagggg tagaagggaa tggactcaag ctaaatatga gcgatatata
     4681 aaagaaggtc gtggccgagg aagtggtaag cactataaac cttggcttac aatccaagat
     4741 gttccctcta aaggacgttc ttctagaact cctggttgga aaacaaatcg agtacatcat
     4801 ttcttctcag accatgaaaa acggctattt tatttgtttg agtggtcaga tatagtcaca
     4861 gatataagag agcaatttcc tttagacaat ctcgatctag ccatgagtat tgctacagac
     4921 ataggtataa agtaccctgt agatacacaa agtggcactc ctcacgtttt aacaagcgac
     4981 tttatgctta cagtcaaccg cgatgggaag cagatgcaga ttgcccggac tgttaagccg
     5041 tcaacagaac tggagaaaaa gcaagttaat gagaaattcg agatagaaag acaatattac
     5101 ttagcaaagg gtattgactg gggaattatc acagaaaaag aaattcccag gcttttagct
     5161 gaaaatgttg agtgggttca ttctgcttat aggctagaag caaattctga aatggacact
     5221 gcacaactga gtgaattgtc aacaatttta aaatctaagc ttcaggaaga agatacaact
     5281 ataaatagag taactactgc tttagacaaa gaaatgaata taaattccgg tacatcactt
     5341 tatgtattta aacatttaat tgctaggaaa gaaatccttg tggatctagt agcaataaaa
     5401 ttatctgctt gtccgtcaac taaagttatt gaaaaaataa tattttagac ccacatgata
     5461 ggtaaataat atggttaaag atttatttgt caatgattta atagagtgga ttgtatacca
     5521 aaacactcat atagtcgaaa gaattatttg gatagatgaa ggttacatta ttgcatttgt
     5581 tatagatatt aatgctaaga aaggtctacc tgaaccaagg aaaatatcag atattttaga
     5641 agctataact gaaggtattg cttttaagca gcaacaagat ccttgggcaa gaattattcg
     5701 ggatgaaaat ttgacagata aggaaaagga atacagagat aaagcttggg agataatttc
     5761 atcattaatt atagaagaac catcaatata ttatagaaat tttagaggat cgatggttaa
     5821 agaagcggtt gaaaagtaca acgaagaaag ggataaagat aaattagtag aaaaaaccgt
     5881 ctatggttat ttgagaagat tttggcagag aggaaaaact ataaatgctc tcatacctga
     5941 ttttatcaat tcatcaggta agggcaagat tcgaggacaa tcgggcaaga agagaggaag
     6001 accaagaaaa tatgcacatg acccagatat tggtgaaggt gtaaatgtta cagaagaaga
     6061 tagaaagatt tttagagtat ctataagcaa attttacaat aatccaaaag aaaactactt
     6121 aacaacagcc tatgaattaa tggtgaaaga atattacaag gaaggaatta gatatgacga
     6181 gaatggtgtt caaaaatcta tattaacccc aactgataaa atacctacaa ttactcaatt
     6241 caaatactgg tatcaacttg aacaggatga tgttaccaaa actttaacat ctcgaaaagg
     6301 agccaagcga gtagctttag agaatagagc aattttggga aactctaaga tggaaactat
     6361 tggccctggt tctagatatc aaattgatgc cacagttgct gatgtatatt tagtttcaaa
     6421 atataaccgt gactggataa ttggtagacc agttatctat gtaattattg atgtttttag
     6481 tagaatgatt acaggtgttt atgtcggttt agaaggccca tcttggatag gagcaatgat
     6541 ggcactagcc aatgtagcta cagataaaac cctgttttgc caggaatata acattaatat
     6601 tactgaaaat gaatggcctt gcaatcaaat gccagatgca attttaggag ataggggaga
     6661 attagcaggt atgacagtag aaacattaat ccctaatctt tatgttcgta ttgaaaatgc
     6721 agcttcctat agagcagatt ggaaagggtt agtagaaaga cactttcgta taattcatga
     6781 atatgtaaaa ccttttttac ctggttatgt ggatacggat tttaggcaaa gaggtgcaag
     6841 ggattatagg cttgatggca aacttgatat agatcaattt actgagatta ttattcgttt
     6901 aattgttcac cataacaata aacctcttac tgaatatgaa agagatgaag caatgattgc
     6961 tgatgatacc cctcctatac ctagagagtt gtggaagtgg ggtatcgcca atcgttctgg
     7021 cagactcaga actttttctg aagatattgt caaattgaac ttgatgccta ctgagaaagc
     7081 aactattact gctaaaggca tcaaaatgag aggtaaggag atgtactata cctgtgatag
     7141 tgctaaaaat gagcaatggt ttgaaaaagc tagaagtaat ctcctttcta agtcagaaaa
     7201 atcattgatt gtatcttatg acatcagaaa gccaaacttc atttatcttc catctagtga
     7261 tggtagagat tttgaaaaat gttttcttat tgacttagaa gggcggtatg ctaataaaaa
     7321 ctttcatgat attgaatatc tgcttgctta tgaagaattg caaagacaga aaagccaagg
     7381 taaacagcta caagaaaagg tagatttaat agctgatatt gaaagcatag tagatagagc
     7441 agaaaaaatg actgatgctt ctcaatatca taatttaagt aagagcaaga aagtttctgg
     7501 aataagagat aatagggctt ttgaaaaggc taaacgacga gagaatgaag gatttgaatt
     7561 agcaattaca gataatcaga gcagttttca ggttgcagag agtacagcag aaacctccga
     7621 acagccagaa tcactacagc caaaccatct gaatcttctt aagaaaaagc gacaggagcg
     7681 aaaacgtgga cagaattgag agtgaactgg ttaaaattcc taatggagaa ctagcagtta
     7741 ttgccagata tatagatcaa aaactgccag aatacaacac taatccatta attcaggcat
     7801 tacctcctat cctatcagca gcagaattta tagataaagt agccagagaa ccaaattttg
     7861 atgaacaaga gagagagcta gaaccacatt atagatttca ttgtattgag aggttatcta
     7921 gatattttga ccctcaaaat aaaactcttg aattacaaaa agttatttgt acgcttatca
     7981 tgacaggcta tctgtcaagg aaccttttaa aacctgaata tgcaaatcgc tctaggcaaa
     8041 tttataatgc aataggtaat ggaggaggta aaaatttaga aaactatgtt aatcttccta
     8101 catctgcgtc cggcttaact ctaattggcc catcaggtat tggaaagact acaaatttca
     8161 aaaatattct tgagctttat ccccaaataa tttttcatcc tgattatagt gtttaccaaa
     8221 tagtttggct gcgggtagat tgccctaaaa ctttaaaggg gttatgtata gatacttttg
     8281 caggtatcga taaattgtta ggaactaatt tattaaagaa atttaaccca gcacgcacat
     8341 ctgaagatta tctattggct caacttgcac aaacagcaca cactcatcat ctaggagttt
     8401 tggtgattga tgaaatacaa aatttagcaa ctgcaaaaag aaatagagac gaaatattaa
     8461 attttttagt aaagctcgat aatactattg gaattccagt tattcgtgta ggtacgaatg
     8521 aagccattag ttttcttcaa ggtaatctta gaaatgctag aagaaatact ggagaaggaa
     8581 gtagaatgtg ggatagaatg gtaaataatg atgaatggtg ttacttttta gaaggaatat
     8641 gggaatacca atggacaaaa acacctgttc atttctcgga tgaaataaaa gaagtttttt
     8701 atgatgaaag ccagggaatt attgatattg ctataaaact ttacaaaatg gttcaatgga
     8761 gagcgatttc aataggtgac caagaaatta ttacggttga tctgattcgt caagtagcta
     8821 aagaaggatt gtatttagta aagccaatgc tagatgcaat taggttaggg gacagagagt
     8881 ggatgattaa atataaggat attgctcctc tcgatattac ggaatatcac aatagatgtg
     8941 tatctgagat tgatgctaaa gatttgacag agattaagag attagctaaa aagcagcaga
     9001 atcagcagaa agtatctccg aagctcaatt atgtaattag tgagctgttg aaattggaag
     9061 tagaatctgg taaagcaaag gaatgtgcag aacaaattgt ttatagtcat gaagatccag
     9121 acattccaat tccatcttta gttaataagg catatgctct tgctttgcaa ggatgtcaag
     9181 taaatgagag tcttgctaaa acaagtacca ggaaagctaa attaaagaca aagtaccaag
     9241 agaatgatat tcggcgaatt gtcgagaatg ccaagaaaga caaaaaatct gcatacgagg
     9301 atttgaaatt agcaggtata atcaaaaatc cagttgaaga ttttatcaat atttagcttc
     9361 taaatgctta gttttttccc aactctgtat cccgatgaac ttttatatag cactttagct
     9421 agataccata ttcggagtgg aaataaaagt ttcaagcaaa ctgacttaga gctattcggt
     9481 tacagttccc aacaaatatg caaaattacc ttaactaata atttaaatta tttagtgaaa
     9541 aacttacctt tacgctccca gcaaaaagtt gagaatttac tacaaaatca tactttatat
     9601 tccttttatg caactttatt aatgccgcag gaggcttggc tgctgaaaaa ctcaatgagt
     9661 aataaattca gtgggtctat actagatttt gctaaagtgg ctaataattc tagtgatgat
     9721 tcaaaaaaat ttttaaaatt ttgccctgaa tgtttagaga aagataccca aaagtatggt
     9781 gaaccttact ggcatagaat acatcaagtc cctggcattt tagtttgtcc aattcatcgt
     9841 gttgttttac acaatagctg tatagaagtt gaatcgaaag gcatacatta tcatgctgcc
     9901 agcatagata attgtttatt agcaaataat gtgaaaaaat tgacaaataa aactgtagaa
     9961 acactttcaa tactggcaca tgatattaat tggctaattc atagcagttt tgcctttcaa
    10021 ggtttggctt ggcttcgcgc tcagtaccag cgttacttat tggataaagg actgctaaaa
    10081 acctttgctg gtggcaagtt taagtttaat gattgtggtt ttgcccagct tatatttgag
    10141 ttttatggtc aagaatcctt agaagccctc aatccaaagc tgacccaaaa tcaaggtaaa
    10201 tatttttctc actgcttgtt tggatgcgac ttaaacccag caattgaccg cataactcat
    10261 atcttaatga taaaattttt agcaaattct atagaagagt tctttcagtc ataacttaag
    10321 tcgaacagaa agtaatcgta ttgtacacgg ccgcataatc gaaattaata cgactcacta
    10381 taggggaatt gtgagcggat aacaattccc catcttagta tattagttaa gtataagaag
    10441 gagatataca tggtgagttt tttccctcaa ccttatccag atgaaatttt atatagtgtt
    10501 attgctcgtt atcacattag aagtggcaat acgagtccaa aaattacatt acaagattta
    10561 tttaagtcac agactactgt ggcgacagta gatttaccat cgaaccttag taatttaatt
    10621 aaaaacctac aatttatctc aaattaccaa gttgaagagt tgatatataa acatactctc
    10681 taccctttat atagcccttt tttgccagca aacaggtcta gtcaggtact ggaatctatg
    10741 aaaggagatt atgggggaga tattcataca agagccggaa ttatggcaag ctcaattact
    10801 atgccgaaat atttgcggtt ttgtccaact tgctgggaag aagacttaaa aaattatggc
    10861 gaaccttact ggcatcgcat ccatcaaata ccagggattt tagtttgtcc atttcatggt
    10921 gaagttttgc aagatagcct agctccttta catggtttta ataagcatga atattatgca
    10981 gctagtacag acaactgttc tattactaaa gaacccagta ttcttagccc tgacacactg
    11041 aaaaaattac taatattagc caaagatttt tggtggttac tcaatagtga aataccttgc
    11101 caagagccac agtggtttcg caaacagtac acaaatctga tgattgagca gggttttgca
    11161 actccaactg gtcgaattta ccagaacaaa ctactagata attttttgtt cttttatggt
    11221 caagaaatgc tcagtgctgt tgattcaatg gtaaatcata atgattcaaa caactgggtt
    11281 actagcatcg tcaggaaata taggaaatct ttccatcctc ttagacatct gctaatgatg
    11341 cgttttttga aaacatcagt agaggaattt tttcaaaaag atactgaata caagcctttt
    11401 ggcgaacccc cctggttgtg tctcaatgct gccgcagaac attatctaca accagtgatt
    11461 accaatttag tcattacttt atgctgcgat acaaaaaagc ctgtcggtac tttttcttgt
    11521 gcttgcggaa tggtttattg ccggactggg cctgatgaaa cggatgaaga caaagttcgc
    11581 attggtaaag tcaaggcatt cggtcaaata tgggagcaaa agctgaaaga attggtggaa
    11641 gttgaaaagt tgggtttgcg ggagacagcc agaagattaa atgttgatgc cagaactatc
    11701 aaacgttatg tatctcgtct aaaattgaca acttattggc agactagaac agaaaatgac
    11761 tctgtaggct tacagggagt cccagaaata gatcccaatt ttgcaactga actcaaatta
    11821 aaacacaggc aaaattggat ggctttacaa gtacagaacc ctgaagcttc aaagacaact
    11881 ctgagaaaac tagtaccagc cacctatatt tggctataca ggaatgacag agaatggctc
    11941 aatcaaaact cacccacttt acaagttgcc gttccttccg ttgtgagagt tgattggcat
    12001 gagcgagatc agcaaattct cgctcaggtt caagatgcag tgcgatcgct acttaaggct
    12061 gatataccag agaaaatttc tattagtagg gtagggaaaa ctatcggctt acttgcatta
    12121 ctcgaaaaac atctcgacca gatgccttta acaaaggctt atttggaatc agtcgttgaa
    12181 actgtagaag aatttcagat gcgccggatc aagtgggcaa tcaagctgct tgatgaatgt
    12241 ggagaagaaa ttatgcgctg gaaggtagtc agagttgctg ctttgcgaga ggattgctcc
    12301 caaagagtca gtgcatttct ggaaagtgaa ctggagaaag catacaagaa agagaggtga
    12361 ttaattaacc taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
    12421 taaacgggtc ttgaggggtt ttttgctgaa acctcaggca tttgagaagc acacggtcac
    12481 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
    12541 accctgccct gaaccgacga caagctgacg accgctgccc tgaaccgacg accgggtcat
    12601 cgtggccgga tcttgcggcc cctcggcttg aacgaattgt tagacattat ttgccgacta
    12661 ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg
    12721 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
    12781 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
    12841 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
    12901 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
    12961 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
    13021 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
    13081 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
    13141 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
    13201 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
    13261 catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc
    13321 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
    13381 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatac
    13441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    13501 tatttgaatg tatttagaaa aataaacaaa tagctagctc actcggtcgc tacgctccgg
    13561 gcgtgagact gcggcgggcg ctgcggacac atacaaagtt acccacagat tccgtggata
    13621 agcaggggac taacatgtga ggcaaaacag cagggccgcg ccggtggcgt ttttccatag
    13681 gctccgccct cctgccagag ttcacataaa cagacgcttt tccggtgcat ctgtgggagc
    13741 cgtgaggctc aaccatgaat ctgacagtac gggcgaaacc cgacaggact taaagatccc
    13801 caccgtttcc ggcgggtcgc tccctcttgc gctctcctgt tccgaccctg ccgtttaccg
    13861 gatacctgtt ccgcctttct cccttacggg aagtgtggcg ctttctcata gctcacacac
    13921 tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca agaactcccc
    13981 gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa cccggaaaag
    14041 cacggtaaaa cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt
    14101 agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag
    14161 atttggttgc tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact
    14221 taaccttcga tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg
    14281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc gctctagatt
    14341 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
    14401 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
    14461 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
    14521 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    14581 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc
    14641 agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag
    14701 cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg
    14761 atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg
    14821 cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc
    14881 agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac
    14941 atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat
    15001 ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc
    15061 gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca
    15121 tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga
    15181 acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg
    15241 atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg
    15301 ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta
    15361 atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc
    15421 agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc
    15481 gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc
    15541 acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact
    15601 ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga
    15661 aaggttttgc gccattcgat ggtgtccgg
//
