LOCUS       sequence-331003-        8986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-dpcoCas9 fused with TV activator for transcriptional 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang
            Y, Cheng Y, Shih PM, Qi Y
  TITLE     CRISPR-Act3.0 for highly efficient multiplexed gene activation in 
            plants.
  JOURNAL   Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. 
            Epub 2021 Jun 24.
  PUBMED    34168320
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00953-7"; journalName: "Nat Plants"; date: 
            "2021-07"; volume: "7"; issue: "7"; pages: "942-953"
COMMENT     Sequence Label: pYPQ-dpcoCas9-TV
FEATURES             Location/Qualifiers
     source          1..8986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             345..1136
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1229..1817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1718..1737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1971..1988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2147..2174)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2266..2352)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2401..2423
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2415..2432
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2416..2432
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2448..2547
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2569..2616
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2623..2643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2668..4104,4294..6957)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          4105..4293
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             6958..7005
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8089..8238
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8251..8400
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      8423..8675
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8720..8843
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8860..8879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8861..8879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8884..8900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8884..8900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
       61 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
      121 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      181 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      241 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      301 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
      361 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      421 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      481 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      541 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      601 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      661 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      721 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      781 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      841 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
      901 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
      961 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1021 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1081 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     1141 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     1201 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1861 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1921 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1981 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2041 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2101 caattaatac gcgtaccgcg agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     2161 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     2221 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     2281 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     2341 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgcttgca tggatgtttt
     2401 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     2461 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     2521 tgccaacttt gtacaaaaaa gcaggctccg aattcgccct tcaccatgga ttacaaggat
     2581 gatgatgata aggattacaa ggatgatgat gataagatgg ctccaaagaa gaagagaaag
     2641 gttggaatcc acggagttcc agctgctgat aagaagtact ctatcggact tgccatcgga
     2701 accaactctg ttggatgggc tgttatcacc gatgagtaca aggttccatc taagaagttc
     2761 aaggttcttg gaaacaccga tagacactct atcaagaaga accttatcgg tgctcttctt
     2821 ttcgattctg gagagaccgc tgaggctacc agattgaaga gaaccgctag aagaagatac
     2881 accagaagaa agaacagaat ctgctacctt caggaaatct tctctaacga gatggctaag
     2941 gttgatgatt ctttcttcca cagacttgag gagtctttcc ttgttgagga ggataagaag
     3001 cacgagagac acccaatctt cggaaacatc gttgatgagg ttgcttacca cgagaagtac
     3061 ccaaccatct accaccttag aaagaagttg gttgattcta ccgataaggc tgatcttaga
     3121 cttatctacc ttgctcttgc tcacatgatc aagttcagag gacacttcct tatcgaggga
     3181 gaccttaacc cagataactc tgatgttgat aagttgttca tccagcttgt tcagacctac
     3241 aaccagcttt tcgaggagaa cccaatcaac gcttctggag ttgatgctaa ggctatcctt
     3301 tctgctagac tttctaagtc tcgtagactt gagaacctta tcgctcagct tccaggagag
     3361 aagaagaacg gacttttcgg aaaccttatc gctctttctc ttggacttac cccaaacttc
     3421 aagtctaact tcgatcttgc tgaggatgct aagttgcagc tttctaagga tacctacgat
     3481 gatgatcttg ataaccttct tgctcagatc ggagatcagt acgctgatct tttccttgct
     3541 gctaagaacc tttctgatgc tatccttctt tctgacatcc ttagagttaa caccgagatc
     3601 accaaggctc cactttctgc ttctatgatc aagagatacg atgagcacca ccaggatctt
     3661 acccttttga aggctcttgt tagacagcag cttccagaga agtacaagga aatcttcttc
     3721 gatcagtcta agaacggata cgctggatac atcgatggag gagcttctca ggaggagttc
     3781 tacaagttca tcaagccaat ccttgagaag atggatggaa ccgaggagct tcttgttaag
     3841 ttgaacagag aggatcttct tagaaagcag agaaccttcg ataacggatc tatcccacac
     3901 cagatccacc ttggagagct tcacgctatc cttcgtagac aggaggattt ctacccattc
     3961 ttgaaggata acagagagaa gatcgagaag atccttacct tcagaatccc atactacgtt
     4021 ggaccacttg ctagaggaaa ctctcgtttc gcttggatga ccagaaagtc tgaggagacc
     4081 atcacccctt ggaacttcga ggaggtaagt ttctgcttct acctttgata tatatataat
     4141 aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg
     4201 tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt
     4261 tctaatatat gaccaaaatt tgttgatgtg caggttgttg ataagggagc ttctgctcag
     4321 tctttcatcg agagaatgac caacttcgat aagaaccttc caaacgagaa ggttcttcca
     4381 aagcactctc ttctttacga gtacttcacc gtttacaacg agcttaccaa ggttaagtac
     4441 gttaccgagg gaatgagaaa gccagctttc ctttctggag agcagaagaa ggctatcgtt
     4501 gatcttcttt tcaagaccaa cagaaaggtt accgttaagc agttgaagga ggattacttc
     4561 aagaagatcg agtgcttcga ttctgttgaa atctctggag ttgaggatag attcaacgct
     4621 tctcttggaa cctaccacga tcttttgaag atcatcaagg ataaggattt ccttgataac
     4681 gaggagaacg aggacatcct tgaggacatc gttcttaccc ttaccctttt cgaggataga
     4741 gagatgatcg aggagagact caagacctac gctcaccttt tcgatgataa ggttatgaag
     4801 cagttgaaga gaagaagata caccggatgg ggtagacttt ctcgtaagtt gatcaacgga
     4861 atcagagata agcagtctgg aaagaccatc cttgatttct tgaagtctga tggattcgct
     4921 aacagaaact tcatgcagct tatccacgat gattctctta ccttcaagga ggacatccag
     4981 aaggctcagg tttctggaca gggagattct cttcacgagc acatcgctaa ccttgctgga
     5041 tctccagcta tcaagaaggg aatccttcag accgttaagg ttgttgatga gcttgttaag
     5101 gttatgggta gacacaagcc agagaacatc gttatcgaga tggctagaga gaaccagacc
     5161 acccagaagg gacagaagaa ctctcgtgag agaatgaaga gaatcgagga gggaatcaag
     5221 gagcttggat ctcaaatctt gaaggagcac ccagttgaga acacccagct tcagaacgag
     5281 aagttgtacc tttactacct tcagaacgga agagatatgt acgttgatca ggagcttgac
     5341 atcaacagac tttctgatta cgatgttgat gccatcgttc cacagtcttt cttgaaggat
     5401 gattctatcg ataacaaggt tcttacccgt tctgataaga acagaggaaa gtctgataac
     5461 gttccatctg aggaggttgt taagaagatg aagaactact ggagacagct tcttaacgct
     5521 aagttgatca cccagagaaa gttcgataac cttaccaagg ctgagagagg aggactttct
     5581 gagcttgata aggctggatt catcaagaga cagcttgttg agaccagaca gatcaccaag
     5641 cacgttgctc agatccttga ttctcgtatg aacaccaagt acgatgagaa cgataagttg
     5701 atcagagagg ttaaggttat caccttgaag tctaagttgg tttctgattt cagaaaggat
     5761 ttccagttct acaaggttag agagatcaac aactaccacc acgctcacga tgcttacctt
     5821 aacgctgttg ttggaaccgc tcttatcaag aagtacccaa agttggagtc tgagttcgtt
     5881 tacggagatt acaaggttta cgatgttaga aagatgatcg ctaagtctga gcaggagatc
     5941 ggaaaggcta ccgctaagta cttcttctac tctaacatca tgaacttctt caagaccgag
     6001 atcacccttg ctaacggaga gatcagaaag agaccactta tcgagaccaa cggagagacc
     6061 ggagagatcg tttgggataa gggaagagat ttcgctaccg ttagaaaggt tctttctatg
     6121 ccacaggtta acatcgttaa gaaaaccgag gttcagaccg gaggattctc taaggagtct
     6181 atccttccaa agagaaactc tgataagttg atcgctagaa agaaggattg ggacccaaag
     6241 aagtacggag gattcgattc tccaaccgtt gcttactctg ttcttgttgt tgctaaggtt
     6301 gagaagggaa agtctaagaa gttgaagtct gttaaggagc ttcttggaat caccatcatg
     6361 gagcgttctt ctttcgagaa gaacccaatc gatttccttg aggctaaggg atacaaggag
     6421 gttaagaagg atcttatcat caagttgcca aagtactctc ttttcgagct tgagaacgga
     6481 agaaagagaa tgcttgcttc tgctggagag cttcagaagg gaaacgagct tgctcttcca
     6541 tctaagtacg ttaacttcct ttaccttgct tctcactacg agaagttgaa gggatctcca
     6601 gaggataacg agcagaagca gcttttcgtt gagcagcaca agcactacct tgatgagatc
     6661 atcgagcaaa tctctgagtt ctctaagaga gttatccttg ctgatgctaa ccttgataag
     6721 gttctttctg cttacaacaa gcacagagat aagccaatca gagagcaggc tgagaacatc
     6781 atccaccttt tcacccttac caaccttggt gctccagctg ctttcaagta cttcgatacc
     6841 accatcgata gaaaaagata cacctctacc aaggaggttc ttgatgctac ccttatccac
     6901 cagtctatca ccggacttta cgagaccaga atcgatcttt ctcagcttgg aggagataag
     6961 agaccagctg ctaccaagaa ggctggacag gctaagaaga agaagggaga cggctctgga
     7021 tcggggtcgg gttctggctc agtcgacttg cttgatccgg ggacaccaat ggacgcggac
     7081 ctggtggctt catcgaccgt ggtttgggaa caggacgccg atccattcgc cgggaccgcc
     7141 gatgactttc ctgcttttaa tgaggaagag ttggcttggc tgatggaact cctgccgcag
     7201 ggcggctcag ggggtctcct tgaccccggc acccccatgg acgctgacct cgttgcaagt
     7261 tcgacggttg tttgggagca agatgcagat ccgtttgcgg gtacagctga tgactttcca
     7321 gccttcaacg aagaggagct ggcatggctt atggagcttc tgcctcaggc cagaggaggg
     7381 tcgggtgggc tcttggatcc tgggacgccg atggatgccg accttgtcgc gtcctctact
     7441 gtggtttggg agcaggacgc cgacccgttc gctggtactg ctgatgactt tccggccttt
     7501 aatgaggagg aactcgcttg gctcatggag ttgttgccac aagggggtag tggcggtctt
     7561 ctggacccgg gaacgcctat ggatgctgac ttggtggcat cgagcaccgt cgtctgggaa
     7621 caggatgcgg acccgtttgc tggtaccgct gatgattttc cggcatttaa cgaggaagaa
     7681 ttggcgtggc tcatggaatt gcttccccag gcgagagggg gttccggtgg cttgctggat
     7741 ccgggaacgc ctatggatgc cgatctcgtg gcgtcgtcaa ccgtggtctg ggagcaagac
     7801 gctgacccgt ttgccggcac agcagatgat tttccagctt tcaatgagga agaactggca
     7861 tggttgatgg aattgcttcc acagggaggg agcggaggcc tcctcgatcc tggaacacct
     7921 atggacgcag acctggttgc ctcttcaact gtcgtgtggg aacaagatgc agacccgttc
     7981 gcaggtacag ctgatgactt ccctgccttt aatgaggagg aactcgcgtg gctcatggaa
     8041 cttttgcccc aagcacgcgg tggctctggg ggtggcggat cgggaggaga tgcgctggac
     8101 gatttcgact tggacatgtt gggctcagac gctttggacg actttgatct cgacatgctt
     8161 gggtccgacg cattggatga tttcgacctt gacatgcttg gttccgacgc actcgatgac
     8221 ttcgatcttg atatgctcgc ccgcggatct gacgctctcg acgacttcga tcttgacatg
     8281 ttgggctcag atgcgctcga cgactttgat ctcgatatgt tgggtagcga cgcactcgat
     8341 gactttgact tggatatgct ggggagcgac gccttggacg atttcgatct ggacatgctg
     8401 taaaagcttt gataggacgt ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     8461 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     8521 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     8581 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     8641 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttgatccccc ctcgacagct
     8701 tccggaaagg gcgaattcgc aactttgtat acaaaagttg aacgagaaac gtaaaatgat
     8761 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     8821 acacaacata tccagtcact atgccatcca gctgatatcc cctatagtga gtcgtattac
     8881 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     8941 gcacaagata aaaatatatc atcatgcctc ctctggacca gccagg
//
