LOCUS       sequence-331004-        9424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-dpcoCas9 fused with EDLL activator for transcriptional 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang
            Y, Cheng Y, Shih PM, Qi Y
  TITLE     CRISPR-Act3.0 for highly efficient multiplexed gene activation in 
            plants.
  JOURNAL   Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. 
            Epub 2021 Jun 24.
  PUBMED    34168320
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00953-7"; journalName: "Nat Plants"; date: 
            "2021-07"; volume: "7"; issue: "7"; pages: "942-953"
COMMENT     Sequence Label: pYPQ-dpcoCas9-EV2.1
FEATURES             Location/Qualifiers
     source          1..9424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(165..192)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(284..370)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     419..441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     433..450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     434..450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    466..565
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             587..634
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             641..661
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(686..2122,2312..4975)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2123..2311
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             4976..5023
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5138..5191
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5195..5581
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             5582..5602
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5645..5665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5669..5689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5693..5713
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5714..5863
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             5864..5884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5888..5908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5909..6265
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6290..6859
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      6879..7131
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    7176..7299
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7316..7335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7317..7335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7340..7356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7340..7356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7787..8578
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8671..9259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9160..9179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9413..9424,1..6)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatacgc gtaccgcgag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
      181 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
      241 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
      301 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
      361 gttttatttg atgcctggca gttccctact ctcgcgttaa cgcttgcatg gatgttttcc
      421 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
      481 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
      541 ccaactttgt acaaaaaagc aggctccgaa ttcgcccttc accatggatt acaaggatga
      601 tgatgataag gattacaagg atgatgatga taagatggct ccaaagaaga agagaaaggt
      661 tggaatccac ggagttccag ctgctgataa gaagtactct atcggacttg ccatcggaac
      721 caactctgtt ggatgggctg ttatcaccga tgagtacaag gttccatcta agaagttcaa
      781 ggttcttgga aacaccgata gacactctat caagaagaac cttatcggtg ctcttctttt
      841 cgattctgga gagaccgctg aggctaccag attgaagaga accgctagaa gaagatacac
      901 cagaagaaag aacagaatct gctaccttca ggaaatcttc tctaacgaga tggctaaggt
      961 tgatgattct ttcttccaca gacttgagga gtctttcctt gttgaggagg ataagaagca
     1021 cgagagacac ccaatcttcg gaaacatcgt tgatgaggtt gcttaccacg agaagtaccc
     1081 aaccatctac caccttagaa agaagttggt tgattctacc gataaggctg atcttagact
     1141 tatctacctt gctcttgctc acatgatcaa gttcagagga cacttcctta tcgagggaga
     1201 ccttaaccca gataactctg atgttgataa gttgttcatc cagcttgttc agacctacaa
     1261 ccagcttttc gaggagaacc caatcaacgc ttctggagtt gatgctaagg ctatcctttc
     1321 tgctagactt tctaagtctc gtagacttga gaaccttatc gctcagcttc caggagagaa
     1381 gaagaacgga cttttcggaa accttatcgc tctttctctt ggacttaccc caaacttcaa
     1441 gtctaacttc gatcttgctg aggatgctaa gttgcagctt tctaaggata cctacgatga
     1501 tgatcttgat aaccttcttg ctcagatcgg agatcagtac gctgatcttt tccttgctgc
     1561 taagaacctt tctgatgcta tccttctttc tgacatcctt agagttaaca ccgagatcac
     1621 caaggctcca ctttctgctt ctatgatcaa gagatacgat gagcaccacc aggatcttac
     1681 ccttttgaag gctcttgtta gacagcagct tccagagaag tacaaggaaa tcttcttcga
     1741 tcagtctaag aacggatacg ctggatacat cgatggagga gcttctcagg aggagttcta
     1801 caagttcatc aagccaatcc ttgagaagat ggatggaacc gaggagcttc ttgttaagtt
     1861 gaacagagag gatcttctta gaaagcagag aaccttcgat aacggatcta tcccacacca
     1921 gatccacctt ggagagcttc acgctatcct tcgtagacag gaggatttct acccattctt
     1981 gaaggataac agagagaaga tcgagaagat ccttaccttc agaatcccat actacgttgg
     2041 accacttgct agaggaaact ctcgtttcgc ttggatgacc agaaagtctg aggagaccat
     2101 caccccttgg aacttcgagg aggtaagttt ctgcttctac ctttgatata tatataataa
     2161 ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta
     2221 gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc
     2281 taatatatga ccaaaatttg ttgatgtgca ggttgttgat aagggagctt ctgctcagtc
     2341 tttcatcgag agaatgacca acttcgataa gaaccttcca aacgagaagg ttcttccaaa
     2401 gcactctctt ctttacgagt acttcaccgt ttacaacgag cttaccaagg ttaagtacgt
     2461 taccgaggga atgagaaagc cagctttcct ttctggagag cagaagaagg ctatcgttga
     2521 tcttcttttc aagaccaaca gaaaggttac cgttaagcag ttgaaggagg attacttcaa
     2581 gaagatcgag tgcttcgatt ctgttgaaat ctctggagtt gaggatagat tcaacgcttc
     2641 tcttggaacc taccacgatc ttttgaagat catcaaggat aaggatttcc ttgataacga
     2701 ggagaacgag gacatccttg aggacatcgt tcttaccctt acccttttcg aggatagaga
     2761 gatgatcgag gagagactca agacctacgc tcaccttttc gatgataagg ttatgaagca
     2821 gttgaagaga agaagataca ccggatgggg tagactttct cgtaagttga tcaacggaat
     2881 cagagataag cagtctggaa agaccatcct tgatttcttg aagtctgatg gattcgctaa
     2941 cagaaacttc atgcagctta tccacgatga ttctcttacc ttcaaggagg acatccagaa
     3001 ggctcaggtt tctggacagg gagattctct tcacgagcac atcgctaacc ttgctggatc
     3061 tccagctatc aagaagggaa tccttcagac cgttaaggtt gttgatgagc ttgttaaggt
     3121 tatgggtaga cacaagccag agaacatcgt tatcgagatg gctagagaga accagaccac
     3181 ccagaaggga cagaagaact ctcgtgagag aatgaagaga atcgaggagg gaatcaagga
     3241 gcttggatct caaatcttga aggagcaccc agttgagaac acccagcttc agaacgagaa
     3301 gttgtacctt tactaccttc agaacggaag agatatgtac gttgatcagg agcttgacat
     3361 caacagactt tctgattacg atgttgatgc catcgttcca cagtctttct tgaaggatga
     3421 ttctatcgat aacaaggttc ttacccgttc tgataagaac agaggaaagt ctgataacgt
     3481 tccatctgag gaggttgtta agaagatgaa gaactactgg agacagcttc ttaacgctaa
     3541 gttgatcacc cagagaaagt tcgataacct taccaaggct gagagaggag gactttctga
     3601 gcttgataag gctggattca tcaagagaca gcttgttgag accagacaga tcaccaagca
     3661 cgttgctcag atccttgatt ctcgtatgaa caccaagtac gatgagaacg ataagttgat
     3721 cagagaggtt aaggttatca ccttgaagtc taagttggtt tctgatttca gaaaggattt
     3781 ccagttctac aaggttagag agatcaacaa ctaccaccac gctcacgatg cttaccttaa
     3841 cgctgttgtt ggaaccgctc ttatcaagaa gtacccaaag ttggagtctg agttcgttta
     3901 cggagattac aaggtttacg atgttagaaa gatgatcgct aagtctgagc aggagatcgg
     3961 aaaggctacc gctaagtact tcttctactc taacatcatg aacttcttca agaccgagat
     4021 cacccttgct aacggagaga tcagaaagag accacttatc gagaccaacg gagagaccgg
     4081 agagatcgtt tgggataagg gaagagattt cgctaccgtt agaaaggttc tttctatgcc
     4141 acaggttaac atcgttaaga aaaccgaggt tcagaccgga ggattctcta aggagtctat
     4201 ccttccaaag agaaactctg ataagttgat cgctagaaag aaggattggg acccaaagaa
     4261 gtacggagga ttcgattctc caaccgttgc ttactctgtt cttgttgttg ctaaggttga
     4321 gaagggaaag tctaagaagt tgaagtctgt taaggagctt cttggaatca ccatcatgga
     4381 gcgttcttct ttcgagaaga acccaatcga tttccttgag gctaagggat acaaggaggt
     4441 taagaaggat cttatcatca agttgccaaa gtactctctt ttcgagcttg agaacggaag
     4501 aaagagaatg cttgcttctg ctggagagct tcagaaggga aacgagcttg ctcttccatc
     4561 taagtacgtt aacttccttt accttgcttc tcactacgag aagttgaagg gatctccaga
     4621 ggataacgag cagaagcagc ttttcgttga gcagcacaag cactaccttg atgagatcat
     4681 cgagcaaatc tctgagttct ctaagagagt tatccttgct gatgctaacc ttgataaggt
     4741 tctttctgct tacaacaagc acagagataa gccaatcaga gagcaggctg agaacatcat
     4801 ccaccttttc acccttacca accttggtgc tccagctgct ttcaagtact tcgataccac
     4861 catcgataga aaaagataca cctctaccaa ggaggttctt gatgctaccc ttatccacca
     4921 gtctatcacc ggactttacg agaccagaat cgatctttct cagcttggag gagataagag
     4981 accagctgct accaagaagg ctggacaggc taagaagaag aagggagacg gctctggatc
     5041 ggggtcgggt tctggctcag tcgacgaagt atttgagttc gagtacctgg acgataaggt
     5101 tcttgaggag ctcttggaca gtgaagagcg taaaagagag ggcagaggaa gtcttctaac
     5161 atgcggtgac gtggaggaga atcccggccc tatggcgtca aatttcacgc agtttgtttt
     5221 ggttgataac ggcgggactg gcgacgttac agtagctcca tcaaattttg cgaacggagt
     5281 cgctgagtgg attagctcaa attcaaggtc ccaggcctac aaggttacct gttctgttag
     5341 gcagagttct gcgcaaaaaa gaaaatatac catcaaggtt gaagtcccta aagttgcaac
     5401 acaaacagtc ggtggtgttg agctccctgt ggcagcctgg agatcttact taaacatgga
     5461 gctaacaatt ccaatattcg ctacaaactc tgattgtgaa ctgattgtta aggcgatgca
     5521 aggtctcttg aaagatggaa accctatacc gtccgctatc gcagctaaca gcggtatcta
     5581 tcctaagaag aagaggaagg ttggctctgg atcggggtcg ggttctggct caggatccgg
     5641 taccccaaaa aagaagagaa aggtcgaccc gaagaagaaa aggaaagtcg accctaaaaa
     5701 aaaacgcaaa gttgatgcgc ttgacgactt tgacttggac atgttgggat cagatgccct
     5761 cgacgacttt gatctcgata tgcttggttc ggacgcgctt gacgatttcg atcttgacat
     5821 gctcggttct gatgcacttg atgacttcga tttggacatg ctgcccaaaa agaaaagaaa
     5881 ggtggatcca aagaagaaac gcaaagtccc tactcaggct ggggaaggga ctctgagtga
     5941 agctctcctg caacttcagt tcgacgacga ggacttgggt gcccttctcg ggaacagcac
     6001 cgacccggct gttttcactg acctcgcgtc tgttgataat tccgaattcc aacagctttt
     6061 gaatcaagga attcctgttg ccccacatac tactgaaccc atgctgatgg aatatcccga
     6121 agcaataact agactggtga ccggtgcgca gcggccgcca gacccagctc cagccccgct
     6181 gggtgcacca ggactgccga atggcttgct gtcaggcgat gaagacttta gttcgatcgc
     6241 tgatatggac ttctctgcac tgcttattaa ttccaggtct tctggtagtc gggactctcg
     6301 cgagggtatg ttcttgccaa aaccagaggc tggatcggca atatctgatg tttttgaggg
     6361 acgggaggtc tgccagccga aacgcatccg cccgtttcac ccgccaggta gcccctgggc
     6421 aaaccgccca ttgcccgcct cgcttgcccc aacccccacc ggtcctgttc acgaacccgt
     6481 cggatctctg accccagccc ctgttccaca gccgttggac ccagccccag cagtcacccc
     6541 ggaggccagt catctcctgg aggacccaga tgaagagacc agtcaggcag ttaaggcttt
     6601 gcgggaaatg gctgacaccg tcatacctca gaaagaagaa gcagccattt gcggacagat
     6661 ggatctgtct catccccctc cccggggtca ccttgatgag ctgacaacaa cgctggagag
     6721 tatgacggaa gatcttaact tggactcacc tctgaccccg gaactcaacg aaatattgga
     6781 tacatttttg aacgatgagt gtctgcttca tgcaatgcac atcagtaccg gactgagtat
     6841 atttgatact agcttgttta agctttgata ggacgtccga tcgttcaaac atttggcaat
     6901 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     6961 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     7021 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     7081 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattga
     7141 tcccccctcg acagcttccg gaaagggcga attcgcaact ttgtatacaa aagttgaacg
     7201 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     7261 tacataatac tgtaaaacac aacatatcca gtcactatgc catccagctg atatccccta
     7321 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     7381 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct ggaccagcca
     7441 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     7501 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     7561 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     7621 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     7681 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     7741 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     7801 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     7861 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     7921 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     7981 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     8041 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     8101 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     8161 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     8221 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     8281 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     8341 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     8401 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     8461 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     8521 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     8581 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     8641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9301 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9421 agtg
//
