LOCUS       sequence-331015-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Scaﬀold design for studying ubiquitin interacting proteins by 
            cryo-EM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P, Hsu SD
  TITLE     Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy
            Aided by a Small Scaffold Protein.
  JOURNAL   Biochemistry. 2021 Apr 13;60(14):1075-1079. doi: 
            10.1021/acs.biochem.0c00961. Epub 2021 Mar 15.
  PUBMED    33719392
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.0c00961"; journalName: "Biochemistry"; date: 
            "2021-04-13- 13"; volume: "60"; issue: "14"; pages: "1075-1079"
COMMENT     Sequence Label: pRSFDuet1-His-TEV-OTC_d1_Ub
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             54..74
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tttcgaatac catgggcagc agccatcacc atcatcacca cggcagcagc ggtgaaaacc
       61 tgtattttca gggatccatg tccgggtttt atcataagca tttcctgaaa ttactcgatt
      121 tcacgccagc tgaactcaac agcctgctgc agttagccgc gaagctgaaa gccgataaga
      181 aaagcggtaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag
      241 actcgactcg tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgcgtta
      301 cttatctcgg cccaagcggc agccagattg gtcataaaga gtcgattaaa gacactgccc
      361 gcgtgcttgg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
      421 aaacactggc ggaatacgct agcgtgccgg tatggaatgg cctgaccaat gagttccatc
      481 ccacgcagct gctggcggat cttctcacca tgcaggagca tttgcccggc aaagcgttca
      541 acgaaatgac gctggtctat gcaggtgacg cgcgtaacaa catgggcaat tcgatgctcg
      601 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaagcg tgctggccgg
      661 aagctgcgct ggttacggaa tgccgcgccc tggcacagca aaatggtggg aatattacgc
      721 tgactgaaga tgtcgcgaag ggagttgaag gtgctgactt tatctatacc gatgtgtggg
      781 tgtcgatggg ggaagcaaaa gagaaatggg cggaacggat tgcattgctg cgtgaatatc
      841 agtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
      901 gcccgcgttt catgacgacc aaacgacgct tggcaagaaa atggcggaag aatttgggcc
      961 tacatggcgg tatgggaagt cactgatgag tcttcgaatc tgccgccagc attgtttttg
     1021 atcagcggaa aaccgtatgc atactatcaa agcggtgatg gtcgcgacgc tcagtat
//
