LOCUS       sequence-331016-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Scaﬀold design for studying ubiquitin interacting proteins by 
            cryo-EM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P, Hsu SD
  TITLE     Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy
            Aided by a Small Scaffold Protein.
  JOURNAL   Biochemistry. 2021 Apr 13;60(14):1075-1079. doi: 
            10.1021/acs.biochem.0c00961. Epub 2021 Mar 15.
  PUBMED    33719392
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.0c00961"; journalName: "Biochemistry"; date: 
            "2021-04-13- 13"; volume: "60"; issue: "14"; pages: "1075-1079"
COMMENT     Sequence Label: pRSFDuet1-His-TEV-OTC_d1_Ub
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..262)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 cagcatcgtc gacttagcat tatgcggccg ctcaaccacc acgaagtctc aacacaagat
       61 gaagagtaga ctccttttga atattgtagt cagacaaagt acgtccatct tccagctgct
      121 tgccagcaaa gatcagtctc tgctgatcag gaggaattcc ttccttatcc tggatcttgg
      181 cctttacatt ttctatcgta tccgagggtt caacctcgag ggtgatggtc ttccccgtaa
      241 gggttttcac gaaaatctgc atactgagcg tcgcgaccat caccgctttg atagtatgca
      301 tacggttttc cgcctgatca aaaacaatgc tggcggcaga ttcgaagacc tcatcagtga
      361 cttccatacc gccatgtagg ccaaattctt ccgccatttt cttgccaagc gtcgtttggt
      421 cgtcatgaaa cgcgggcagg cagtggagga atttgacctc cgggttaccg gtcaactgca
      481 tcatcttgct gttcacctga tattcacgca gcaatgcaat ccgttccgcc catttctctt
      541 ttgcttcccc catcgacacc cacacatcgg tatagataaa gtcagcacct tcaactccct
      601 tcgcgacatc ttcagtcagc gtaatattcc caccattttg ctgtgccagg gcgcggcatt
      661 ccgtaaccag cgcagcttcc ggccagcacg cttgtggcgc gaccagacgc aaatccagac
      721 cggtaagcgc cgcagcttcg agcatcgaat tgcccatgtt gttacgcgcg tcacctgcat
      781 agaccagcgt catttcgttg aacgctttgc cgggcaaatg ctcctgcatg gtgagaagat
      841 ccgccagcag ctgcgtggga tggaactcat tggtcaggcc attccatacc ggcacgctag
      901 cgtattccgc cagtgtttcg acaatctcct gaccatagcc gcgatactga ataccgtcat
      961 acatgcgacc aagcacgcgg gcagtgtctt taatcgactc tttatgacca atctggctgc
     1021 cgcttggccg agataagtaa cgcgagcacc cctggtcata tgcggcaact tcgaaaagaa
     1081 g
//
