LOCUS       sequence-331018-        1299 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Scaﬀold design for studying ubiquitin interacting proteins by 
            cryo-EM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Chiu YH, Ko KT, Yang TJ, Wu KP, Ho MR, Draczkowski P, Hsu SD
  TITLE     Direct Visualization of a 26 kDa Protein by Cryo-Electron Microscopy
            Aided by a Small Scaffold Protein.
  JOURNAL   Biochemistry. 2021 Apr 13;60(14):1075-1079. doi: 
            10.1021/acs.biochem.0c00961. Epub 2021 Mar 15.
  PUBMED    33719392
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.0c00961"; journalName: "Biochemistry"; date: 
            "2021-04-13- 13"; volume: "60"; issue: "14"; pages: "1075-1079"
COMMENT     Sequence Label: pRSFDuet1-His-TEV-OTC_d1_Ub
FEATURES             Location/Qualifiers
     source          1..1299
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1069..1296
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 atgggcagca gccatcacca tcatcaccac ggcagcagcg gtgaaaacct gtattttcag
       61 ggatccatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt cacgccagct
      121 gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa aagcggtaaa
      181 gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt
      241 acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgcgttac ttatctcggc
      301 ccaagcggca gccagattgg tcataaagag tcgattaaag acactgcccg cgtgcttggt
      361 cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga aacactggcg
      421 gaatacgcta gcgtgccggt atggaatggc ctgaccaatg agttccatcc cacgcagctg
      481 ctggcggatc ttctcaccat gcaggagcat ttgcccggca aagcgttcaa cgaaatgacg
      541 ctggtctatg caggtgacgc gcgtaacaac atgggcaatt cgatgctcga agctgcggcg
      601 cttaccggtc tggatttgcg tctggtcgcg ccacaagcgt gctggccgga agctgcgctg
      661 gttacggaat gccgcgccct ggcacagcaa aatggtggga atattacgct gactgaagat
      721 gtcgcgaagg gagttgaagg tgctgacttt atctataccg atgtgtgggt gtcgatgggg
      781 gaagcaaaag agaaatgggc ggaacggatt gcattgctgc gtgaatatca ggtgaacagc
      841 aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct gcccgcgttt
      901 catgacgacc aaacgacgct tggcaagaaa atggcggaag aatttggcct acatggcggt
      961 atggaagtca ctgatgaggt cttcgaatct gccgccagca ttgtttttga tcaggcggaa
     1021 aaccgtatgc atactatcaa agcggtgatg gtcgcgacgc tcagtaaaat gcagattttc
     1081 gtgaaaaccc ttacggggaa gaccatcacc ctcgaggttg aaccctcgga tacgatagaa
     1141 aatgtaaagg ccaagatcca ggataaggaa ggaattcctc ctgatcagca gagactgatc
     1201 tttgctggca agcagctgga agatggacgt actttgtctg actacaatat tcaaaaggag
     1261 tctactcttc atcttgtgtt gagacttcgt ggtggttga
//
