LOCUS       sequence-331035-       13351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial lactate oxidase (LOx) enzyme in astrocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13351)
  AUTHORS   Vaccari Cardoso B, Shevelkin AV, Terrillion C, Mychko O, Mosienko V,
            Kasparov S, Pletnikov MV, Teschemacher AG
  TITLE     Reducing l-lactate release from hippocampal astrocytes by 
            intracellular oxidation increases novelty induced activity in mice.
  JOURNAL   Glia. 2021 Jan 5. doi: 10.1002/glia.23960.
  PUBMED    33400321
REFERENCE   2  (bases 1 to 13351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Glia. 2021 
            Jan 5. doi: 10.1002/glia.23960."
COMMENT     Sequence Label: pTYF-sGFAP-LOx-IRES-tdTomato
FEATURES             Location/Qualifiers
     source          1..13351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    11..132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(22..41)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     76..95
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(375..917)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(981..1421)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(1458..1476)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1522..1560)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3932..5362
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(5450..5470)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5868..5879)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6181..6405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6538..6557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(7579..7596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7750..8338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7830..7849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8509..9300,9301..9369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9370..9474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9542..9560
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9598..9620)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11040..11060
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11103..11283
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12665..12898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    13160..13277
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagtcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
       61 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      121 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      181 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcat
      241 gaacagactg tgaggactga ggggcctgaa atgagccttg ggactgtgaa tctaaaatac
      301 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaattgga tctacttatt
      361 caagatcggc cgctggagct gatctgactc agcagagcag agaagtccat gtccgcaatg
      421 gaggagaagt cttcatctcc ggagagacca ttgggaagcc ccgaggtccc caggggtgtg
      481 ggagctgggt cagggggcct ctgggaccct gtcaccaggc gagttatagc ttcagggtac
      541 tccatcagca tgggctcagc tgtggagtga gacatggaca caccctggtt caggagctgc
      601 tgaaactctg agttgtccac agatgccagg tctgtgaaca ctcctgggtc tgtgctgttg
      661 ccaagcaagg cccccaagtc ttcatcagca tcaaactgca ggtgcagcag ggcttccgac
      721 agcgtgcctt ccccagcctg ggtgctcttt ggaacaggtg cagacaggga ctggggagga
      781 cccggggtta gaactggggc aggagctggg ggctgagcca gaggtaccat ggctgaggaa
      841 gggaccatgg tctgggcaag gactggggca gaggacggtg ctaaggccag ggcctggttt
      901 gagatctgcc cagaaggaaa caccatggtg agctctgcag aagcttctag aattccagcc
      961 cgggcggatc ccaattccgg cgatacagtc aactgtcttt gacctttgtt actactctct
     1021 tccgatgatg atgtcgcact tattctatgc tgtctcaatg ttagaggcat atcagtctcc
     1081 actgaagcca atctatctgt gacggcatct ttattcacat tatcttgtac aaataatcct
     1141 gttaacaatg cttttatatc ctgtaaagaa tccattttca aaatcatgtc aaggtcttct
     1201 cgaggaaaaa tcagtagaaa tagctgttcc agtctttcta gccttgattc cacttctgtc
     1261 agatgtgccc tagtcagcgg agaccttttg gttttgggag agtagcgaca ctcccagttg
     1321 ttcttcagac acttggcgca cttcggtttt tctttggagc acttgagctt tttaagtcgg
     1381 caaatatcgc atgcttgttc gatagaagac agtagcttca tctttcagga ggctagctta
     1441 gatcccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcgctagcg
     1501 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctaac
     1561 gcgctagcct tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
     1621 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagagggacg
     1681 cgtaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg ggggcctgag
     1741 ctggctctgt gagctgggga ggaggcagac agccaggcct tgtctgcaag cagacctggc
     1801 agcattgggc tggccgcccc ccagggcctc ctcttcatgc ccagtgaatg actcaccttg
     1861 gcacagacac aatgttcggg gtgggcacag tgcctgcttc ccgccgcacc ccagcccccc
     1921 tcaaatgcct tccgagaagc ccattgagca gggggcttgc attgcacccc agcctgacag
     1981 cctggcatct tgggataaaa gcagcacagc cccctagggg ctgcccttgc tgtgtggcgc
     2041 caccggcggt ggagaacaag gctctattca gcctgtgccc aggaaagggg atcaggggat
     2101 gcccaggcat ggacagtggg tggcaggggg ggagaggagg gctgtctgct tcccagaagt
     2161 ccaaggacac aaatgggtga ggggagagct ctccccatag ctgggctgcg gcccaacccc
     2221 accccctcag gctatgccag ggggtgttgc caggggcacc cgggcatcgc cagtctagcc
     2281 cactccttca taaagccctc gcatcccagg agcgagcaga gccagagcag gttggagagg
     2341 agacgcatca cctccgctgc tcgcactagc gctaccggac tcagatctcg agctcaagct
     2401 tcgaagccgc caccatgaac aacaacgaca tcgagtacaa cgcccctagc gagatcaagt
     2461 acatcgacgt ggtcaacacc tacgacctgg aagaggaagc cagcaaggtt gtccctcacg
     2521 gcggcttcaa ctatatcgct ggcgctagcg gagatgagtg gaccaagaga gccaacgaca
     2581 gagcctggaa gcacaagctg ctgtacccta gactggccca ggatgtggaa gcccctgaca
     2641 cctctacaga gatcctggga cacaagatca aggccccttt catcatggcc cctatcgccg
     2701 ctcatggact ggctcacaca acaaaagagg ccggcacagc cagagccgtg tctgagtttg
     2761 gcaccatcat gagcatcagc gcctacagcg gcgccacctt cgaggaaatc tctgagggac
     2821 tgaacggcgg acccagatgg ttccaaatct acatggccaa ggacgaccag cagaacagag
     2881 acatcctgga cgaggccaag tctgatggcg ccacagccat catcctgaca gccgactcta
     2941 ccgtgtccgg caacagagat agggacgtga agaacaagtt cgtgtacccc ttcggcatgc
     3001 ccatcgtgca gagatacctg agaggcacag ccgagggcat gagcctgaac aacatctacg
     3061 gcgccagcaa gcagaagatc agccccaggg acatcgaaga gatcgccgga cattctggcc
     3121 tgcctgtgtt cgtgaagggc atccagcatc ctgaggacgc cgacatggct atcaagagag
     3181 gcgcttccgg aatctgggtg tccaaccatg gtgccagaca gctgtacgag gcccctggct
     3241 ctttcgacac actgcctgct atcgccgagc gcgtgaacaa gagggtgcca atcgtgttcg
     3301 atagcggcgt gcggagaggc gagcatgtgg ctaaagctct tgcctctggc gccgatgttg
     3361 tggctcttgg tagacccgtg ctgttcggac tggcacttgg aggatggcag ggcgcttaca
     3421 gcgtgctgga ctacttccag aaagacctga ccagagtgat gcagctgacc ggctctcaga
     3481 acgtcgagga cctgaaaggc ctggacctgt tcgacaaccc ctacggctac gagtactgag
     3541 atatctgcag tcgacggtac cagatggggg tcctgggccc cagggtgtgc agccactgac
     3601 ttggggactg ctggtggggt agggatgagg gagggagggg cattgtgatg tacagggctg
     3661 ctctgtgaga tcaagggtct cttaagggtg ggagctgggg cagggactac gagagcagcc
     3721 agatgggctg aaagtggaac tcaaggggtt tctggcacct acctacctgc ttcccgctgg
     3781 ggggtgggga gttggcccag agtcttaaga ttggggcagg gtggagaggt gggctcttcc
     3841 tgcttcccac tcatcttata gctttctttc cccagatccg aattcgagat ccaaaccaag
     3901 ggtaccgcgg gcccgggatc caccggtcgc catggtgagc aagggcgagg aggtcatcaa
     3961 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
     4021 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     4081 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
     4141 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
     4201 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
     4261 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     4321 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     4381 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     4441 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     4501 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     4561 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     4621 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     4681 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     4741 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4801 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     4861 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     4921 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     4981 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     5041 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     5101 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     5161 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     5221 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     5281 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     5341 catggacgag ctgtacaagt aggcggccgc cccgttattt gcactctgtt cctgttaatc
     5401 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5461 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5521 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5581 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5641 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5701 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5761 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5821 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5881 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5941 ttcgccctca gacgagtcgg atctcccttt gggccgccta tcgattaggg ataacagggt
     6001 aacggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6061 agaaaagggg ggactggaag ggctaattca ctcccagggt ctctctggtt agaccagatc
     6121 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca taaagctcga
     6181 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6241 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6301 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6361 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6421 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     6481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctgtgcggga gaacggagtt
     6601 ctattatgac tcaaatcagt ctccccaagc attcggggat cagagttttt aaggataact
     6661 tagtgtgtag ggggccagtg agttggagat gaaagcgtag ggagtcgaag gtgtcctttt
     6721 gcgccgagtc agttcctggg tgggggccac aagatcggat gagccagttt atcaatccgg
     6781 gggtgccagc tgatccatgg agtgcagggt ctgcaaaata tctcaagcac tgattgatct
     6841 taggttttac aatagtgatg ttaccccagg aacaatttgg ggaaggtcag aatcttgtag
     6901 cctgtagctg catgactcct aaaccataat ttcttttttg tttttttttt tttatttttg
     6961 agacagggtc tcactctgtc acctaggctg gagtgcagtg gtgcaatcac agctcactgc
     7021 agcctcaacg tcgtaagctc aagcgatcct cccacctcag cctgcctggt agctgagact
     7081 acaagcgacg ccccagttaa tttttgtatt tttggtagag gcagcgtttt gccgtgtggc
     7141 cctggctggt ctcgaactcc tgggctcaag tgatccagcc tcagcctccc aaagtgctgg
     7201 gacaaccggg gccagtcact gcacctggcc ctaaaccata atttctaatc ttttggctaa
     7261 tttgttagtc ctacaaaggc agtctagtcc ccaggcaaaa agggggtttg tttcgggaaa
     7321 gggctgttac tgtctttgtt tcaaactata aactaagttc ctcctaaact tagttcggcc
     7381 tacacccagg aatgaacaag gagagcttgg aggttagaag cacgatggaa ttggttaggt
     7441 cagatctctt tcactgtctg agttataatt ttgcaatggt ggttcaaaga ctgcccgctt
     7501 ctgacaccag tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7561 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8161 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8401 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8461 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8521 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8581 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8641 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8701 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8761 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8821 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8881 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8941 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9001 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9061 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9121 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9181 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9241 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9301 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9361 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9421 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9481 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9541 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     9601 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     9661 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     9721 ggcatcagag cagattgtac kgrgmkkgcw ymactatgcg gcatcagagc agattgtact
     9781 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9841 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     9901 ttcgctatta cgccagggga ggcagagatt gcagtaagct gagatcgcag cactgcactc
     9961 cagcctgggc gacagagtaa gactctgtct caaaaataaa ataaataaat caatcagata
    10021 ttccaatctt ttcctttatt tatttattta ttttctattt tggaaacaca gtccttcctt
    10081 attccagaat tacacatata ttctattttt ctttatatgc tccagttttt tttagacctt
    10141 cacctgaaat gtgtgtatac aaaatctagg ccagtccagc agagcctaaa ggtaaaaaat
    10201 aaaataataa aaaataaata aaatctagct cactccttca catcaaaatg gagatacagc
    10261 tgttagcatt aaataccaaa taacccatct tgtcctcaat aattttaagc gcctctctcc
    10321 accacatcta actcctgtca aaggcatgtg ccccttccgg gcgctctgct gtgctgccaa
    10381 ccaactggca tgtggactct gcagggtccc taactgccaa gccccacagt gtgccctgag
    10441 gctgcccctt ccttctagcg gctgccccca ctcggctttg ctttccctag tttcagttac
    10501 ttgcgttcag ccaaggtctg aaactaggtg cgcacagagc ggtaagactg cgagagaaag
    10561 agaccagctt tacagggggt ttatcacagt gcaccctgac agtcgtcagc ctcacagggg
    10621 gtttatcaca ttgcaccctg acagtcgtca gcctcacagg gggtttatca cagtgcaccc
    10681 ttacaatcat tccatttgat tcacaatttt tttagtctct actgtgccta acttgtaagt
    10741 taaatttgat cagaggtgtg ttcccagagg ggaaaacagt atatacaggg ttcagtacta
    10801 tcgcatttca ggcccgagag ctgcatccgg agtatctaga tggagttccg cgttacataa
    10861 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    10921 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggagttt
    10981 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
    11041 gcaaatgggc ggtaggcgtg tactctagaa ggtctatata agcagagctc gtttagtgaa
    11101 ccgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    11161 cactgcttaa gcctcaataa agcttgcctt gagtgctcaa agtagtgtgt gcccgtctgt
    11221 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    11281 gcagtggcgc ccgaacaggg acttgaaagc gaaagtaaag ccagaggaga tctctcgacg
    11341 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tgcagagtac
    11401 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcggtat
    11461 taagcggggg agaattagat aaatgggaaa aaattcggtt aaggccaggg ggaaagaaac
    11521 aatataaact aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    11581 ctggcctttt agagacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    11641 ttcagacagg atcagaagaa cttagatcat tatataatac aatagcagtc ctctattgtg
    11701 tgcatcaaag gatagatgta aaagacacca aggaagcctt agataagata gaggaagagc
    11761 aaaacaaaag taagaaaaag gcacagcaag cagcagctga cacaggaaac aacagccagg
    11821 tcagccaaaa ttaccctata gtgcagaacc tccaggggca aatggtacat caggccatat
    11881 cacctagaac tttaaatgca tgggtaaaag tagtagaaga gaaggctttc agcccagaag
    11941 taatacccat gttttcagca ttatcagaag gagccacccc acaagattta aataccatgc
    12001 taaacacagt ggggggacat caagcagcca tgcaaatgtt aaaagagacc atcaatgagg
    12061 aagctgcaga atgggataga ttgcatccag tgcatgcagg gcctattgca ccaggccaga
    12121 tgagagaacc aaggggaagt gacatagcag gaactactag caaattaaga gaacaatttg
    12181 gaaataataa aacaataatc tttaagcaat cctcaggagg ggacccagaa attgtaacgc
    12241 acagttttaa ttgtggaggg gaatttttct actgtaattc aacacaactg tttaatagta
    12301 cttggtttaa tagtacttgg agtactgaag ggtcaaataa cactgaagga agtgacacaa
    12361 tcacactccc atgcagaata aaacaattta taaacatgtg gcaggaagta ggaaaagcaa
    12421 tgtatgcccc tcccatcagt ggacaaatta gatgttcatc aaatattact gggctgctat
    12481 taacaagaga tggtggtaat aacaacaatg ggtccgagat cttcagacct ggaggaggcg
    12541 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    12601 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    12661 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    12721 caatgacgct gacggtacag gccagacaat tattgtctga tatagtgcag cagcagaaca
    12781 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    12841 aacagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    12901 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    12961 ggagtaataa atctctggaa cagatttgga ataacatgac ctggatggag tgggacagag
    13021 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    13081 aaaagaatga acaagaatta ttggaattag ataaatgggc ttgcggccgg aattcacaaa
    13141 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    13201 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    13261 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tgggcggcct
    13321 agggataaca gggtaatgat atctctgacc g
//
