LOCUS       sequence-331038-       12212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Vaccari Cardoso B, Shevelkin AV, Terrillion C, Mychko O, Mosienko V,
            Kasparov S, Pletnikov MV, Teschemacher AG
  TITLE     Reducing l-lactate release from hippocampal astrocytes by 
            intracellular oxidation increases novelty induced activity in mice.
  JOURNAL   Glia. 2021 Jan 5. doi: 10.1002/glia.23960.
  PUBMED    33400321
REFERENCE   2  (bases 1 to 12212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Glia. 2021 
            Jan 5. doi: 10.1002/glia.23960."
COMMENT     Sequence Label: pTYF-sGFAP-IRES-tdTomato
FEATURES             Location/Qualifiers
     source          1..12212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    11..132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(22..41)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     76..95
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(375..917)
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             complement(981..1421)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(1458..1476)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1522..1560)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2793..4223
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(4311..4331)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4729..4740)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5042..5266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5399..5418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(6440..6457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6611..7199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6691..6710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7370..8161,8162..8230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8231..8335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8403..8421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8459..8481)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9901..9921
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9964..10144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11526..11759
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12021..12138
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagtcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
       61 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      121 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      181 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcat
      241 gaacagactg tgaggactga ggggcctgaa atgagccttg ggactgtgaa tctaaaatac
      301 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaattgga tctacttatt
      361 caagatcggc cgctggagct gatctgactc agcagagcag agaagtccat gtccgcaatg
      421 gaggagaagt cttcatctcc ggagagacca ttgggaagcc ccgaggtccc caggggtgtg
      481 ggagctgggt cagggggcct ctgggaccct gtcaccaggc gagttatagc ttcagggtac
      541 tccatcagca tgggctcagc tgtggagtga gacatggaca caccctggtt caggagctgc
      601 tgaaactctg agttgtccac agatgccagg tctgtgaaca ctcctgggtc tgtgctgttg
      661 ccaagcaagg cccccaagtc ttcatcagca tcaaactgca ggtgcagcag ggcttccgac
      721 agcgtgcctt ccccagcctg ggtgctcttt ggaacaggtg cagacaggga ctggggagga
      781 cccggggtta gaactggggc aggagctggg ggctgagcca gaggtaccat ggctgaggaa
      841 gggaccatgg tctgggcaag gactggggca gaggacggtg ctaaggccag ggcctggttt
      901 gagatctgcc cagaaggaaa caccatggtg agctctgcag aagcttctag aattccagcc
      961 cgggcggatc ccaattccgg cgatacagtc aactgtcttt gacctttgtt actactctct
     1021 tccgatgatg atgtcgcact tattctatgc tgtctcaatg ttagaggcat atcagtctcc
     1081 actgaagcca atctatctgt gacggcatct ttattcacat tatcttgtac aaataatcct
     1141 gttaacaatg cttttatatc ctgtaaagaa tccattttca aaatcatgtc aaggtcttct
     1201 cgaggaaaaa tcagtagaaa tagctgttcc agtctttcta gccttgattc cacttctgtc
     1261 agatgtgccc tagtcagcgg agaccttttg gttttgggag agtagcgaca ctcccagttg
     1321 ttcttcagac acttggcgca cttcggtttt tctttggagc acttgagctt tttaagtcgg
     1381 caaatatcgc atgcttgttc gatagaagac agtagcttca tctttcagga ggctagctta
     1441 gatcccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcgctagcg
     1501 gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctaac
     1561 gcgctagcct tgcatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc
     1621 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagactc tagagggacg
     1681 cgtaacatat cctggtgtgg agtaggggac gctgctctga cagaggctcg ggggcctgag
     1741 ctggctctgt gagctgggga ggaggcagac agccaggcct tgtctgcaag cagacctggc
     1801 agcattgggc tggccgcccc ccagggcctc ctcttcatgc ccagtgaatg actcaccttg
     1861 gcacagacac aatgttcggg gtgggcacag tgcctgcttc ccgccgcacc ccagcccccc
     1921 tcaaatgcct tccgagaagc ccattgagca gggggcttgc attgcacccc agcctgacag
     1981 cctggcatct tgggataaaa gcagcacagc cccctagggg ctgcccttgc tgtgtggcgc
     2041 caccggcggt ggagaacaag gctctattca gcctgtgccc aggaaagggg atcaggggat
     2101 gcccaggcat ggacagtggg tggcaggggg ggagaggagg gctgtctgct tcccagaagt
     2161 ccaaggacac aaatgggtga ggggagagct ctccccatag ctgggctgcg gcccaacccc
     2221 accccctcag gctatgccag ggggtgttgc caggggcacc cgggcatcgc cagtctagcc
     2281 cactccttca taaagccctc gcatcccagg agcgagcaga gccagagcag gttggagagg
     2341 agacgcatca cctccgctgc tcgcactagc gctaccggac tcagatctcg agctcaagct
     2401 tcgatctgca gtcgacggta ccagatgggg gtcctgggcc ccagggtgtg cagccactga
     2461 cttggggact gctggtgggg tagggatgag ggagggaggg gcattgtgat gtacagggct
     2521 gctctgtgag atcaagggtc tcttaagggt gggagctggg gcagggacta cgagagcagc
     2581 cagatgggct gaaagtggaa ctcaaggggt ttctggcacc tacctacctg cttcccgctg
     2641 gggggtgggg agttggccca gagtcttaag attggggcag ggtggagagg tgggctcttc
     2701 ctgcttccca ctcatcttat agctttcttt ccccagatcc gaattcgaga tccaaaccaa
     2761 gggtaccgcg ggcccgggat ccaccggtcg ccatggtgag caagggcgag gaggtcatca
     2821 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     2881 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     2941 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     3001 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     3061 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     3121 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     3181 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     3241 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     3301 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     3361 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     3421 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     3481 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     3541 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     3601 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3661 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     3721 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     3781 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     3841 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     3901 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     3961 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     4021 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     4081 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     4141 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     4201 gcatggacga gctgtacaag taggcggccg ccccgttatt tgcactctgt tcctgttaat
     4261 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4321 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4381 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4441 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4501 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4561 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4621 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4681 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4741 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4801 cttcgccctc agacgagtcg gatctccctt tgggccgcct atcgattagg gataacaggg
     4861 taacggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4921 aagaaaaggg gggactggaa gggctaattc actcccaggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc ataaagctcg
     5041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5281 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctgtgcggg agaacggagt
     5461 tctattatga ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac
     5521 ttagtgtgta gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt
     5581 tgcgccgagt cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg
     5641 ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc
     5701 ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta
     5761 gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt ttttattttt
     5821 gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca cagctcactg
     5881 cagcctcaac gtcgtaagct caagcgatcc tcccacctca gcctgcctgg tagctgagac
     5941 tacaagcgac gccccagtta atttttgtat ttttggtaga ggcagcgttt tgccgtgtgg
     6001 ccctggctgg tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc caaagtgctg
     6061 ggacaaccgg ggccagtcac tgcacctggc cctaaaccat aatttctaat cttttggcta
     6121 atttgttagt cctacaaagg cagtctagtc cccaggcaaa aagggggttt gtttcgggaa
     6181 agggctgtta ctgtctttgt ttcaaactat aaactaagtt cctcctaaac ttagttcggc
     6241 ctacacccag gaatgaacaa ggagagcttg gaggttagaa gcacgatgga attggttagg
     6301 tcagatctct ttcactgtct gagttataat tttgcaatgg tggttcaaag actgcccgct
     6361 tctgacacca gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7201 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8341 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     8401 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     8461 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8521 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     8581 cggcatcaga gcagattgta ctgagagkgc wymactatgc ggcatcagag cagattgtac
     8641 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     8701 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8761 cttcgctatt acgccagggg aggcagagat tgcagtaagc tgagatcgca gcactgcact
     8821 ccagcctggg cgacagagta agactctgtc tcaaaaataa aataaataaa tcaatcagat
     8881 attccaatct tttcctttat ttatttattt attttctatt ttggaaacac agtccttcct
     8941 tattccagaa ttacacatat attctatttt tctttatatg ctccagtttt ttttagacct
     9001 tcacctgaaa tgtgtgtata caaaatctag gccagtccag cagagcctaa aggtaaaaaa
     9061 taaaataata aaaaataaat aaaatctagc tcactccttc acatcaaaat ggagatacag
     9121 ctgttagcat taaataccaa ataacccatc ttgtcctcaa taattttaag cgcctctctc
     9181 caccacatct aactcctgtc aaaggcatgt gccccttccg ggcgctctgc tgtgctgcca
     9241 accaactggc atgtggactc tgcagggtcc ctaactgcca agccccacag tgtgccctga
     9301 ggctgcccct tccttctagc ggctgccccc actcggcttt gctttcccta gtttcagtta
     9361 cttgcgttca gccaaggtct gaaactaggt gcgcacagag cggtaagact gcgagagaaa
     9421 gagaccagct ttacaggggg tttatcacag tgcaccctga cagtcgtcag cctcacaggg
     9481 ggtttatcac attgcaccct gacagtcgtc agcctcacag ggggtttatc acagtgcacc
     9541 cttacaatca ttccatttga ttcacaattt ttttagtctc tactgtgcct aacttgtaag
     9601 ttaaatttga tcagaggtgt gttcccagag gggaaaacag tatatacagg gttcagtact
     9661 atcgcatttc aggcccgaga gctgcatccg gagtatctag atggagttcc gcgttacata
     9721 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9781 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggagtt
     9841 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
     9901 cgcaaatggg cggtaggcgt gtactctaga aggtctatat aagcagagct cgtttagtga
     9961 accgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10021 ccactgctta agcctcaata aagcttgcct tgagtgctca aagtagtgtg tgcccgtctg
    10081 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10141 agcagtggcg cccgaacagg gacttgaaag cgaaagtaaa gccagaggag atctctcgac
    10201 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctgcagagta
    10261 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcggta
    10321 ttaagcgggg gagaattaga taaatgggaa aaaattcggt taaggccagg gggaaagaaa
    10381 caatataaac taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    10441 cctggccttt tagagacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    10501 cttcagacag gatcagaaga acttagatca ttatataata caatagcagt cctctattgt
    10561 gtgcatcaaa ggatagatgt aaaagacacc aaggaagcct tagataagat agaggaagag
    10621 caaaacaaaa gtaagaaaaa ggcacagcaa gcagcagctg acacaggaaa caacagccag
    10681 gtcagccaaa attaccctat agtgcagaac ctccaggggc aaatggtaca tcaggccata
    10741 tcacctagaa ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa
    10801 gtaataccca tgttttcagc attatcagaa ggagccaccc cacaagattt aaataccatg
    10861 ctaaacacag tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag
    10921 gaagctgcag aatgggatag attgcatcca gtgcatgcag ggcctattgc accaggccag
    10981 atgagagaac caaggggaag tgacatagca ggaactacta gcaaattaag agaacaattt
    11041 ggaaataata aaacaataat ctttaagcaa tcctcaggag gggacccaga aattgtaacg
    11101 cacagtttta attgtggagg ggaatttttc tactgtaatt caacacaact gtttaatagt
    11161 acttggttta atagtacttg gagtactgaa gggtcaaata acactgaagg aagtgacaca
    11221 atcacactcc catgcagaat aaaacaattt ataaacatgt ggcaggaagt aggaaaagca
    11281 atgtatgccc ctcccatcag tggacaaatt agatgttcat caaatattac tgggctgcta
    11341 ttaacaagag atggtggtaa taacaacaat gggtccgaga tcttcagacc tggaggaggc
    11401 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    11461 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    11521 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    11581 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg atatagtgca gcagcagaac
    11641 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    11701 aaacagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    11761 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    11821 tggagtaata aatctctgga acagatttgg aataacatga cctggatgga gtgggacaga
    11881 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    11941 gaaaagaatg aacaagaatt attggaatta gataaatggg cttgcggccg gaattcacaa
    12001 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    12061 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    12121 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttgggcggcc
    12181 tagggataac agggtaatga tatctctgac cg
//
