LOCUS       sequence-331039-        5706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-169a-16X
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(932..948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(932..948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(945..967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    956..972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(980..986,987..1004,1005..1010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1025..1046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1163..1180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1334..1922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1414..1433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2093..2884,2885..2953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2716..2735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2954..3058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3126..3144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3182..3204)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3304..3323
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3517..3539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3531..3548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3532..3548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattggtcgt agacagctct agcaccgctt aaacgcacgt acggaatcag atcctgcagc
       61 tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
      121 cgcttaaacg cacgtacgga ttcagatcct gcagcttttt tttttcacaa tcccggtgcc
      181 gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt acggaatcag
      241 atcctgcagc tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca
      301 gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagcttttt tttttcacaa
      361 tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt aaacgcacgt
      421 acggaatcag atcctgcagc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
      481 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagcttttt
      541 tttttcacaa tcccggtgcc gaggccgctc aattggtcgt agacagctct agcaccgctt
      601 aaacgcacgt acggaatcag atcctgcagc tttttttttt cacaatcccg gtgccgaggc
      661 cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct
      721 gcagcttttt tttttcacaa tcccggtgcc gaggccgctc aattggtcgt agacagctct
      781 agcaccgctt aaacgcacgt acggaatcag atcctgcagc tttttttttt cacaatcccg
      841 gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgga
      901 ttcagatctg cagaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2221 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2701 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3061 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3121 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     3181 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     3241 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     3301 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     3361 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga attcgagctc ggtacccggg gatcctgcag cttttttttt tcacaatccc
     3601 ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgg
     3661 aatcagatcc tgcagctttt ttttttcaca atcccggtgc cgaggccgct caattggtcg
     3721 tagacagctc tagcaccgct taaacgcacg tacggattca gatcctgcag cttttttttt
     3781 tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac
     3841 gcacgtacgg aatcagatcc tgcagctttt ttttttcaca atcccggtgc cgaggccgct
     3901 caattggtcg tagacagctc tagcaccgct taaacgcacg tacggattca gatcctgcag
     3961 cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
     4021 ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt ttttttcaca atcccggtgc
     4081 cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg tacggattca
     4141 gatcctgcag cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
     4201 agctctagca ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt ttttttcaca
     4261 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
     4321 tacggattca gatcctgcag cttttttttt tcacaatccc ggtgccgagg ccgctcaatt
     4381 ggtcgtagac agctctagca ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt
     4441 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     4501 taaacgcacg tacggattca gatcctgcag cttttttttt tcacaatccc ggtgccgagg
     4561 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgg aatcagatcc
     4621 tgcagctttt ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc
     4681 tagcaccgct taaacgcacg tacggattca gatcctgcag cttttttttt tcacaatccc
     4741 ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgg
     4801 aatcagatcc tgcagctttt ttttttcaca atcccggtgc cgaggccgct caattggtcg
     4861 tagacagctc tagcaccgct taaacgcacg tacggattca gatcctgcag cttttttttt
     4921 tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac
     4981 gcacgtacgg aatcagatcc tgcagctttt ttttttcaca atcccggtgc cgaggccgct
     5041 caattggtcg tagacagctc tagcaccgct taaacgcacg tacggattca gatcctgcag
     5101 cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
     5161 ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt ttttttcaca atcccggtgc
     5221 cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg tacggattca
     5281 gatcctgcag cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
     5341 agctctagca ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt ttttttcaca
     5401 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
     5461 tacggattca gatcctgcag cttttttttt tcacaatccc ggtgccgagg ccgctcaatt
     5521 ggtcgtagac agctctagca ccgcttaaac gcacgtacgg aatcagatcc tgcagctttt
     5581 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     5641 taaacgcacg tacggattca gatcctgcag cttttttttt tcacaatccc ggtgccgagg
     5701 ccgctc
//
