LOCUS       sequence-331050-       20592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Clone encoding rabbit ryanodine receptor in mammalian expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20592)
  AUTHORS   Nakai J, Sekiguchi N, Rando TA, Allen PD, Beam KG
  TITLE     Two regions of the ryanodine receptor involved in coupling with 
            L-type Ca2+ channels.
  JOURNAL   J Biol Chem. 1998 May 29;273(22):13403-6.
  PUBMED    9593671
REFERENCE   2  (bases 1 to 20592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-05-29"; volume: "273(22)"; pages: "13403-6"
COMMENT     Sequence Label: pCIneo RYR-1
FEATURES             Location/Qualifiers
     source          1..20592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2384..2393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    15183..15304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15194..15213)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     15248..15267
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      15490..15945
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15577..15596)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15787..15808
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        16062..16419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        16062..16253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(16085..16105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      16270..16405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             16470..17264
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(16524..16543)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     17134..17153
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    17328..17376
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(17417..17436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17536..17558
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(17596..17614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17682..17786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17787..17855,17856..18647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(18005..18024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      18818..19406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     19307..19326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        19617..19996
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        19997..20208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     20159..20179
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     20205..20229
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          20369..20501
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     20484..20503
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        20546..20564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      join(20587..20592,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggtgacg gaggagaggg cgaggacgaa gtccagttcc tgcggacgga cgacgaggtg
       61 gtcctgcagt gcagcgccac cgtcctcaag gagcagctga agctgtgcct ggccgccgag
      121 ggcttcggca accgcctctg cttcctggag cccaccagca acgcccagaa tgtgcccccc
      181 gacctggcca tctgctgctt caccctggag cagtccctgt cggtccgcgc cctgcaggag
      241 atgctggcca acaccgtgga ggccggcgtg gagtcatccc agggcggggg acacaggaca
      301 ctcctgtatg gccatgccat cctgctccgg catgcacaca gccgcatgta cctgagctgc
      361 ctcacgacct cgcgctccat gactgacaag ctggcttttg acgtgggact gcaggaggat
      421 gcaacaggag aggcctgttg gtggaccatg cacccagcct ccaagcagag atcggaagga
      481 gagaaggtcc gcgtcgggga cgacctcatc cttgtcagcg tctcctccga gcgctacctg
      541 cacctgtcga cggccagtgg ggagctccag gttgatgcct ccttcatgca gaccctgtgg
      601 aacatgaacc ccatctgctc ctgctgtgaa gagggctacg tgactggggg ccacgtcctc
      661 cgcctcttcc acggacacat ggacgagtgc ttgactattt ctgctgctga cagcgacgac
      721 cagcgcaggc ttgtctacta cgagggggga gctgtgtgca cgcacgcccg ctccctctgg
      781 aggctggaac ccctgagaat cagctggagt ggaagccacc tgcgctgggg ccagccactg
      841 cggatccggc atgtcaccac cggacggtac ctggcactca ccgaggacca gggcctggtg
      901 gtggtcgatg cctgcaaggc ccacaccaag gccacctcct tctgtttccg tgtctccaag
      961 gagaagctgg acacggcccc caagcgagac gtggagggca tgggcccgcc tgagatcaag
     1021 tacggggagt cgctgtgctt tgtgcagcac gtggcctcgg gcctgtggct cacctacgcc
     1081 gcccctgacc ccaaggccct gcggctgggc gtgctgaaga agaaggccat tctgcaccag
     1141 gagggccaca tggacgatgc actgttcctg acccgctgcc agcaggagga gtcccaggct
     1201 gcccgcatga tccacagcac tgccggcctg tacaaccagt tcatcaaggg cctggacagc
     1261 ttcagcggga agccccgggg ctcagggccg ccggctggcc ctgcgctgcc catcgaggcc
     1321 gtcatcctga gcctgcagga cctcattggc tacttcgagc cgccctcgga ggagctgcag
     1381 cacgaggaaa agcagagcaa gctgcgcagc ctgcgcaacc gccagagcct cttccaggag
     1441 gaggggatgc tctccctggt cctgaactgc atcgaccgcc tgaacgtcta caccaccgct
     1501 gcccactttg ccgagtatgc aggggaagag gcagctgagt cctggaaaga gattgttaac
     1561 ctactctacg aactcctggc ctctctgatc cggggcaatc gtgccaactg tgccctcttc
     1621 tccacaaact tggactgggt ggtcagcaaa ctggaccggc tggaggcctc gtctggcatc
     1681 ctggaggtgc tgtactgtgt cctgattgag agtcccgagg tgctgaacat catccaggag
     1741 aaccacatca agtccatcat ctccctcctg gacaagcatg ggaggaacca caaggttctg
     1801 gatgtgctgt gctccctgtg tgtgtgcaat ggcgtggctg tgcgctccaa ccaagatctc
     1861 atcaccgaga atctgctccc tggccgtgag ctgctgctgc agacaaacct tatcaactac
     1921 gtcaccagca tccgccccaa catcttcgtg ggccgagccg agggctccac gcagtatggc
     1981 aaatggtact ttgaggtgat ggtggacgag gtggttccat ttctgacagc tcaggccacc
     2041 catctgcggg tgggctgggc cctcaccgag ggctacagcc cctaccctgg cggcggcgag
     2101 ggctggggcg gcaatggggt cggcgatgac ctctactcct atggctttga cgggctgcac
     2161 ctctggacag gacacgtggc acgcccggtg acctcccccg ggcagcacct cctggcccct
     2221 gaggacgtgg tcagctgctg cctggacctc agcgtgccgt ccatctcctt ccgcatcaat
     2281 ggctgccccg tgcagggtgt ctttgaggcc ttcaacctgg atgggctctt cttccctgtg
     2341 gtcagcttct cggctggtgt caaggtgcgg tttctccttg gcggccgcca tggtgaattc
     2401 aagttcctgc ccccacccgg ctacgcccca tgccatgagg cagtgctccc gcgagagcga
     2461 ctccgtcttg agcccatcaa ggagtacaga cgggaagggc cgcgggggcc ccacctggtg
     2521 ggccccagcc gctgcctctc gcacactgac tttgtgccct gcccggtgga caccgtccag
     2581 atcgtcctgc ctccccacct ggagcgcatc cgtgagaagc tggcagagaa catccatgaa
     2641 ctctgggcgc tgacgcgcat tgagcaaggc tggacctatg gcccggttcg ggatgacaac
     2701 aagaggctgc acccgtgtct tgtgaacttc cacagccttc cggagcccga gaggaattac
     2761 aacctgcaga tgtcgggcga gactctcaag actctgctgg cgctgggctg ccacgtgggc
     2821 atggccgacg agaaggcgga ggacaacctg aagaagacaa agctgcccaa gacgtatatg
     2881 atgagcaatg ggtacaagcc agccccgctg gacttgagcc atgtgcgact gacacccgca
     2941 cagacgacac tggtggaccg gctggcagag aatgggcaca acgtgtgggc acgagaccga
     3001 gtggcccagg gctggagcta cagcgccgtg caggacatcc cggctcgccg gaaccctcgc
     3061 cttgtgccct accgcctgct ggacgaggcc accaagcgca gcaaccggga cagcctctgc
     3121 caggccgtgc gcaccctgct gggctatggc tacaacatcg agccacctga ccaggagccc
     3181 agtcaggtgg agaaccagtc tcgctgggac cgggtgcgca tcttccgggc agagaaatcc
     3241 tacacagtgc agagtggccg ctggtacttc gagtttgagg ccgtcaccac aggcgagatg
     3301 cgagtgggct gggcgaggcc cgagctgcgg cctgacgtcg agctgggagc ggatgagctg
     3361 gcctacgtct tcaacgggca ccgtggtcag cgctggcact tgggcagtga gccgttcggg
     3421 cggccctggc agtcaggcga tgtcgtcggc tgcatgatcg acctcaccga gaacaccatc
     3481 atcttcaccc tcaacggcga ggtcctcatg tctgactcgg gctccgaaac agccttccgg
     3541 gagatcgaga ttggggacgg cttcctgcct gtctgcagct tgggacctgg ccaggtgggt
     3601 cacctgaacc tgggccagga tgtgagctcc ctgcggttct ttgccatctg cggcctccag
     3661 gaaggctttg agccattcgc catcaacatg cagcgccctg tcaccacgtg gttcagcaaa
     3721 agccttcccc agtttgagcc tgtgccccct gaacacccgc actatgaggt ggcgcgcatg
     3781 gatggcactg tggacacgcc cccctgtctg cgcctggctc accgcacctg gggctcccag
     3841 aacagcctgg tggagatgct gttcctccgg ctgagccttc cggtccagtt ccatcagcac
     3901 ttccgttgca ccgcaggggc cacgcccttg gcacctcccg gcctgcaacc cccggcggag
     3961 gacgaggccc gggcagcaga acccgaccct gactacgaaa acctgcgccg ctcagctggg
     4021 ggctggggcg aggctgaagg gggcaaagag ggaactgcca aggaggggac acccgggggc
     4081 accccccagc ctggggtgga ggcccagccc gtcagggcgg agaacgagaa ggatgccacc
     4141 acggagaaga acaagaagag agggttctta ttcaaggcca agaaggccgc catgatgact
     4201 cagccaccag ccacccctgc tctgccccgg ctccctcacg acgtggtgcc tgctgacaac
     4261 cgcgatgacc ccgagatcat cctcaacacc accacgtact attactccgt gagggtcttc
     4321 gcgggtcagg agcccagctg cgtatgggtg ggctgggtca cccccgacta ccatcagcac
     4381 gacatgaact ttgacctcag caaggtccgg gcagtgacgg tgaccatggg ggatgaacaa
     4441 ggcaacgtcc acagcagcct caagtgcagc aactgctaca tggtgtgggg tggggacttc
     4501 gtgagccctg ggcagcaggg ccggatcagc cacacggacc ttgtcattgg ctgcctggtg
     4561 gacctggcca ctggcttaat gaccttcacg gccaatggca aagagagcaa cacctttttc
     4621 caggtggaac ccaacacaaa gctctttcct gccgtgtttg tcctgcccac gcaccagaac
     4681 gtcatccaat ttgagctggg gaagcagaag aacatcatgc cgctgtccgc cgccatgttc
     4741 ctgagcgagc gcaagaaccc agcgccacag tgtccaccac ggctggaggt gcagatgctg
     4801 atgcccgtgt cctggagccg catgcccaac cacttcctgc aggtggagac gcggcgcgct
     4861 ggcgagcgcc ttggctgggc cgtgcagtgc caggatcccc tgaccatgat ggcgctgcac
     4921 attccggagg agaaccggtg catggacatc ctggagctgt ccgagcgcct ggacctgcag
     4981 cgcttccact cgcacacgct gcgcctctac cgcgccgtgt gcgccctggg caacaaccgt
     5041 gtggcgcatg ctctgtgcag ccacgtggac caggcgcagc tgctgcacgc gctggaggac
     5101 gcgcacctgc ccggccccct gcgcgcgggc tactacgacc tcctcatcag catccacctg
     5161 gaaagtgcct gccgcagccg gcgctccatg ctctccgagt acatcgtgcc gctcacgccc
     5221 gagacccgcg ccatcacgct cttcccgccg ggaaggaagg ggggaaacgc ccgccgccac
     5281 ggcctgcccg gcgtcggcgt caccacctcg ctgcggcctc cgcaccattt ctcccctccg
     5341 tgcttcgtgg ccgccctgcc cgccgccggg gtagcggaag ccccggcccg cctcagccct
     5401 gccattcctc tggaggccct gcgcgacaag gcgctgagga tgctgggaga ggccgtgcgc
     5461 gatggcgggc agcacgcccg cgaccccgtc gggggctccg tggagttcca gttcgtgccg
     5521 gtgctcaagc tcgtgtccac cctgctggtg atgggcatct ttggcgatga ggatgtgaag
     5581 cagatcttaa agatgataga acctgaagtt ttcaccgagg aagaggagga ggaagaagag
     5641 gaggaggagg aagaagagga ggaagaggag gacgaagagg aaaaggaaga ggacgaggag
     5701 gaagaggaga aggaagatgc tgaaaaagag gaagaggagg ccccggaagg ggaaaaagaa
     5761 gatttggagg aggggctgct ccagatgaag ttgccggagt ccgtgaagct acagatgtgc
     5821 aacctcctgg agtatttctg tgaccaagag ctacagcatc gagtggagtc cctggcggcc
     5881 ttcgcagagc gctacgtgga caagctccag gccaaccagc gcagccgcta cgccctcctc
     5941 atgagagcct tcaccatgag tgcagctgag accgcaaggc gcacccgcga gttccgctcc
     6001 ccgccccagg agcagatcaa catgctcttg cacttcaaag atgaggcaga tgaggaagac
     6061 tgccctctcc ccgaggacat tcgacaggac ttgcaggact ttcatcaaga cctgctggcg
     6121 cactgtggaa tccagctgga gggggaggag gaggagccgg aggaggagac cagcctgagc
     6181 agccgcctcc ggagcctgtt ggagacggtg cggctggtga agaagaagga ggaaaaaccc
     6241 gaggaggagc tgccagccga ggagaaaaaa ccccagtccc tgcaggagct ggtgtcccac
     6301 atggtggtgc gctgggccca ggaggactac gtgcagagcc ctgagctggt gcgggccatg
     6361 ttcagcctcc tgcaccggca gtacgatggg ctcggcgagc tgctgcgcgc cctgccccgg
     6421 gcctacacca tctcgccgtc ctctgtggag gacaccatga gcctgctcga gtgcctcggc
     6481 cagatccgct cgctgctcat cgtgcagatg ggcccccagg aggagaacct catgatccag
     6541 agcattggga acatcatgaa taacaaagtc ttctaccaac acccgaacct gatgagggcc
     6601 ctgggcatgc atgagaccgt catggaggtc atggtgaacg tcctgggggg cggcgaaacc
     6661 aaggagatcc gcttccccaa gatggtgaca agctgctgcc gcttcctctg ctacttctgc
     6721 cgcatcagcc ggcagaacca gcgctccatg tttgaccacc tgagctacct gctggagaac
     6781 agcggcatcg gcctgggtat gcaaggctcc acgcccctgg atgtggctgc cgcctccgtt
     6841 atcgacaaca acgagctggc cttggcgttg caggagcagg acctggaaaa ggtcgtgtcc
     6901 tacctggcag gctgtggcct ccaaagctgc cccatgctcc tggccaaggg ctacccggac
     6961 atcggctgga acccctgcgg tggggagcgc tacctggact tcctgcgctt cgctgtcttt
     7021 gttaacggcg agagcgtgga ggagaacgcc aacgtggtgg tgcggctgct catccgcaaa
     7081 cctgagtgct tcgggcctgc gctgcggggt gagggcggct cggggctgct ggccgccatc
     7141 gaggaggcca tccgcatctc tgaggacccc gcgcgggacg gcccaggcgt ccgcagggac
     7201 cggcggcgcg agcactttgg ggaggagccc cctgaagaaa accgcgtgca cctgggccac
     7261 gccatcatgt ccttctatgc tgccttgatc gacctgctgg gccgctgtgc tccggagatg
     7321 catctgatcc aagccggcaa gggtgaggcc ctgcgaatcc gcgccatcct tcgctccctc
     7381 gtgcccctgg acgacctcgt gggcatcatc agcctcccgc tgcagatccc caccctgggc
     7441 aaagacgggg ccctggtgca gccgaagatg tcggcctcct tcgtgccgga ccacaaggca
     7501 tccatggtgc tcttcctgga ccgcgtgtac ggcattgaga accaggactt cctgctgcac
     7561 gtgctggacg tggggttcct tcccgacatg agagctgccg cctcgctgga cacggccact
     7621 ttcagcacca cggagatggc gctggcgctg aaccgctacc tgtgcctggc tgtgctgccg
     7681 ctcatcacca agtgtgcgcc gctctttgcg ggcacggagc accgtgccat catggtggac
     7741 tcgatgctgc acaccgtgta ccgcctgtcg cgcggccgct cgctcaccaa ggcgcagcgc
     7801 gacgtcatcg aggactgtct catggcgctg tgcaggtaca tccgcccgtc catgctgcag
     7861 cacctgctgc ggcgcctggt gttcgacgtg cccatcctga acgagttcgc caagatgccg
     7921 ctcaagctcc tcaccaacca ctacgagcgc tgctggaagt actactgcct ccccacgggc
     7981 tgggccaact tcggggtcac ctcggaggag gagctgcacc tcacacgcaa actcttctgg
     8041 ggcatcttcg actctctggc ccacaagaag tacgaccagg agctgtaccg catggccatg
     8101 ccttgtctgt gcgccatcgc gggggccctg cctcccgact acgtggacgc ttcgtactct
     8161 tccaaggcgg agaaaaaggc cacagtggat gctgaaggca actttgatcc ccggcctgtg
     8221 gagaccctca atgtgatcat cccggagaag ctcgactcct tcatcaacaa gtttgccgaa
     8281 tacacgcacg agaagtgggc cttcgacaag atccagaaca actggtcgta cggagagaac
     8341 gtggatgagg aactgaagac ccaccccatg ctgaggccct acaagacctt ttcggagaag
     8401 gacaaagaga tctaccgctg gcccatcaag gagtccctga aggccatgat tgcctgggag
     8461 tggacaatcg agaaggccag ggaaggcgag gaggagagga cggagaagaa aaagacacgg
     8521 aagatttccc agactgccca gacctacgat ccccgagaag gctacaaccc gcagcccccc
     8581 gacctcagcg gagtcaccct gtctcgggag ttgcaggcca tggccgaaca actggcggaa
     8641 aactaccata acacctgggg gagaaagaag aagcaggagc tggaagccaa gggcggcggg
     8701 acccaccccc tgctggtccc ctacgacacg ctcacggcca aggagaaagc gcgagaccga
     8761 gagaaggccc aggagctgct gaagttcctg cagatgaacg gctacgcggt caccagaggc
     8821 ctgaaggaca tggagctgga cacgtcttcc attgagaagc ggttcgcctt tggcttcctg
     8881 cagcagttgc tgcgctggat ggacatttcg caggagttca ttgcccacct ggaggctgtg
     8941 gtcagcagtg gacgagtgga gaagtcccca cacgaacagg agattaaatt ctttgccaag
     9001 atcctgctcc ctttgattaa ccagtacttc accaaccact gcctctactt cctgtccaca
     9061 cccgccaaag tgctgggcag tggtggccat gcctccaaca aggagaagga gatgatcacc
     9121 agcctcttct gcaaactcgc tgcccttgtc cgccaccgag tctctctctt cggcacagac
     9181 gccccggctg tggtcaactg ccttcacatc ctggcacgct ccctggatgc caggacggtg
     9241 atgaagtcgg gccccgagat cgtcaaggcc ggcctccgct ccttctttga gagcgcctcg
     9301 gaggacatcg agaagatggt ggagaacttg cggctgggca aggtgtccca ggcgcgcacg
     9361 caggtgaagg gcgtgggcca gaacctcacc tacaccaccg tggccctgct gcccgtcctc
     9421 accacgctct tccagcacat cgcccagcac cagttcggcg acgacgtcat cctggatgac
     9481 gtgcaggtgt cttgctaccg aacattgtgc agcatctact ctctggggac caccaagaac
     9541 acttatgtgg aaaagctacg gccagccctt ggagagtgcc tggcccgcct ggcagcggcc
     9601 atgcctgtgg ccttcctgga gcctcagctg aacgagtaca acgcttgctc cgtgtacacc
     9661 accaagtctc cccgggagcg agccatcctg gggctcccca acagcgtgga ggagatgtgt
     9721 cctgacatcc cggtgctgga tcggctcatg gcagacatcg gggggctggc cgagtccggg
     9781 gcccgctaca cggagatgcc gcacgtcatc gagatcacgc tgcccatgct ctgcagctac
     9841 ctgccccgct ggtgggagcg cgggcccgag gcgccccctc ccgccctgcc tgccggcgcc
     9901 cccccgccct gcacggctgt cacctccgac cacctcaact cactcttggg gaacatcttg
     9961 cgcatcattg tcaacaacct gggcattgac gaggccacct ggatgaagcg gcttgctgtg
    10021 ttcgcccagc ccatcgtgag ccgggcgcgg cctgagctcc tgcattccca cttcatcccc
    10081 accatcgggc ggctgcgcaa gcgggcaggc aaggtggtgg ccgaggagga gcagctgcgg
    10141 ctggaggcca aggccgaggc cgaggagggc gagctcctgg tgcgggacga gttctctgtg
    10201 ctctgccggg acctgtacgc cctctacccg ctgctcatcc gctacgtgga caacaacagg
    10261 gcgcactggc tgacggagcc caacgccaac gccgaggagc tgttcaggat ggtgggcgag
    10321 atcttcatct actggtccaa gtcccacaac ttcaagcgtg aggagcagaa ctttgtggtc
    10381 cagaacgaga tcaacaacat gtccttcctg acggctgaca gcaagagcaa gatggccaag
    10441 gcgggagacg cacagtcggg cggctcggac caggaacgca ccaagaagaa gcgccggggg
    10501 gacaggtact ccgtgcagac gtcactgatc gtggccacgc tgaagaagat gctgcccatt
    10561 ggcctgaaca tgtgtgcgcc caccgaccag gacctcatca tgctggccaa gacccgctac
    10621 gccctgaaag acacagacga ggaggtccgg gaatttctgc agaacaacct ccaccttcag
    10681 ggaaaggtcg aaggctcccc gtccctgcgc tggcagatgg cgctgtaccg gggcctcccc
    10741 ggccgcgagg aggacgccga cgacccggag aaaatcgtgc gcagggtcca agaagtgtca
    10801 gcggtgctct atcacctgga acagaccgag cacccctaca agtccaagaa ggccgtgtgg
    10861 cacaagctgt tgtccaagca gcgccggcga gccgtggtgg cctgcttccg tatgacgccc
    10921 ctgtacaacc tgcccacgca ccgggcctgt aacatgttct tggagagcta caaagccgcc
    10981 tggatcctga ccgaagatca cagtttcgag gaccgcatga tagatgatct ttcaaaagct
    11041 ggggagcagg aggaggagga ggaggaagtg gaggagaaga agccagaccc cctgcaccag
    11101 ctggtcctgc acttcagccg cacggccctg actgaaaaga gcaaactgga cgaggactac
    11161 ctgtacatgg catatgccga catcatggcc aagagctgcc acctggagga gggaggggag
    11221 aacggtgaag ctgaggaaga ggaggttgag gtctcctttg aggagaaaga gatggagaag
    11281 cagaggctcc tctaccagca gtcacggctg cacacgcgcg gggccgccga gatggtgctg
    11341 cagatgatca gcgcctgcaa aggagagaca ggtgccatgg tgtcctccac cctgaagctg
    11401 ggcatctcca tcctcaatgg aggcaatgcc gaggtgcagc agaaaatgct ggattatctg
    11461 aaggacaaga aggaagtcgg cttcttccag agcatccagg cgctgatgca gacatgcagc
    11521 gtcctggatc tcaatgcctt cgagagacag aacaaggcag aaggcctggg catggtgaac
    11581 gaggatggaa ccgtcatcaa tcgccagaac ggagagaagg ttatggccga tgacgaattc
    11641 acacaagatc tgttccgatt tctgcagctg ctctgtgagg ggcacaacaa cgatttccaa
    11701 aactacttac ggacacagac gggcaacacc accacgatta acatcattat ctgcacagtg
    11761 gactacctcc tgcggctgca ggagtccatc agcgacttct actggtacta ctccggcaag
    11821 gatgtcattg aggagcaggg caagaggaac ttctccaagg ccatgtccgt ggccaaacag
    11881 gtgttcaaca gcctcaccga gtacatccag ggcccctgca ccgggaacca gcagagcctg
    11941 gcgcacagcc gcctctggga cgccgtggtg ggattcctgc acgtgtttgc ccacatgatg
    12001 atgaagctcg ctcaggactc gagccagatc gagctgttga aggagctgct ggacctgcag
    12061 aaggacatgg tggtgatgct gctgtcccta ctggaaggga acgtggtgaa cggcatgatt
    12121 gcccggcaga tggtggacat gctggtggag tcctcgtcca acgtggagat gatcctcaag
    12181 ttcttcgaca tgttcctgaa actcaaggac atcgtgggct ccgaggcctt ccaggactac
    12241 gtcaccgatc cccgtggcct catctccaaa aaggacttcc agaaggccat ggacagccag
    12301 aagcagttca ctgggccgga aatccagttt ctgctctcgt gctccgaagc cgacgagaat
    12361 gagatgatca acttcgagga gttcgccaac cgcttccagg agccagcccg ggacatcggc
    12421 ttcaacgtgg ccgtgctgct gaccaacctg tcggagcacg tgccgcacga cccgcgcctg
    12481 cgcaacttcc tggagctggc tgagagcatc cttgagtact tccggcccta cctgggccgc
    12541 atcgagatca tgggcgcctc ccgccgcatc gagcgcatct acttcgagat ctcagagacc
    12601 aaccgcgccc agtgggagat gccccaggtc aaggagtcca agcgccagtt catcttcgac
    12661 gtggtcaacg agggcgggga ggccgagaag atggagctgt tcgtgagctt ctgcgaggac
    12721 accatcttcg agatgcagat cgccgcgcag atctccgagc ccgagggcga gcccgaggcc
    12781 gacgaggacg agggcatggg cgaggcggcc gccgagggcg ccgaggaggg cgcggcgggc
    12841 gccgagggcg cggcgggcac ggtggcggcg ggcgccacgg cgcggctggc ggcggccgcg
    12901 gcccgggcgc tgcgcggcct cagctaccgc agcctgcggc ggcgcgtgcg gcggctgcgg
    12961 cggctcacgg cgcgcgaggc ggccacggcg ctggccgcgc tgctctgggc cgtggtggcg
    13021 cgcgcgggcg cggcgggcgc gggcgcggcg gcgggcgcgc tgcgcctgct ctggggctcg
    13081 ctcttcggcg gcggcctggt ggagggcgcc aagaaggtga cggtgaccga gctgctggcc
    13141 ggcatgcccg atcccaccag cgacgaggtg cacggcgagc agccggccgg gcccggcggc
    13201 gacgcggacg gcgcgggcga gggcgaaggc gagggcgacg cggccgaggg tgacggcgat
    13261 gaggaggtgg cgggtcacga ggccggcccg ggcggtgccg agggggtcgt ggccgtggcc
    13321 gacgggggcc ccttccggcc cgagggcgct ggcggccttg gggacatggg cgacaccacg
    13381 ccggcggaac cgcccacgcc cgagggctcc ccgatcctca agaggaagct gggggtggat
    13441 ggagaagagg aggagctggt tccagaaccg gagcccgagc ccgagcctga gcctgagaaa
    13501 gccgatgagg agaacgggga gaaggaggaa gtccccgagg ccccaccaga accccccaag
    13561 aaagcccctc cctcacctcc tgcaaagaag gaggaagccg gaggcgccgg catggagttc
    13621 tggggagaac tggaagtgca gagggtgaag ttcttgaact acctctcccg gaacttctac
    13681 accctgcgct tccttgccct cttcttggcg tttgccatca acttcatcct gctgttttat
    13741 aaggtctcag actctccacc aggggaggat gacatggagg gctcggcagc aggggacctg
    13801 gcgggtgcag ggtccggggg cggctccggc tggggctcgg gggccggcga ggaggcagag
    13861 ggggacgagg acgagaacat ggtatactac ttcctggagg agagcacggg ctacatggag
    13921 ccggccctgt ggtgcctgag cctgctgcac acgctggtgg ccttcctctg catcatcggc
    13981 tacaactgcc tcaaggtgcc cctggtgatc ttcaagcggg agaaggagct ggcacgaaag
    14041 ctggagtttg acggcctgta catcacagag cagcctgggg acgacgacgt gaagggccag
    14101 tgggaccggc tggtgctcaa cacgccgtct ttccccagca actactggga caagtttgtc
    14161 aagcggaagg tcctggacaa acacggggac atcttcgggc gggagcggat tgccgagctg
    14221 ctgggcatgg acctggcctc gctggagatc acggcccaca acgagcgcaa gcccgacccc
    14281 ccgccggggc tgctgacctg gctcatgtcc atcgatgtca agtaccagat ctggaagttc
    14341 ggggtcatct tcacggacaa ctcgttcctg tacctgggct ggtacatggt gatgtccctc
    14401 ctgggccact acaacaactt cttctttgcc gcccacctgc tggacatcgc catgggggtc
    14461 aagacgctgc gtaccatcct ctcctctgtc acccacaatg ggaaacagct ggtgatgact
    14521 gtgggcctcc tggccgtcgt ggtctacctg tacactgtgg tggccttcaa cttcttccgc
    14581 aagttctaca acaagagcga ggacgaggac gagccggaca tgaagtgcga tgacatgatg
    14641 acgtgctacc tgttccacat gtacgtgggc gtccgggctg gcggaggcat cggggacgag
    14701 atcgaggacc cagcgggcga tgaatacgag ctctaccggg tggtcttcga catcaccttc
    14761 ttcttcttcg tcattgtcat cctgctggcc atcatccagg gtctgattat cgacgccttc
    14821 ggcgagctcc gagaccagca ggagcaagtg aaggaagata tggagaccaa atgcttcatc
    14881 tgcgggattg gcagtgacta cttcgatacc acgccgcacg gcttcgagac ccacacgcta
    14941 gaggagcaca atctggccaa ttacatgttc ttcttgatgt atctgataaa caaggacgag
    15001 acggagcaca cgggccagga gtcctacgtc tggaagatgt atcaggagag gtgctgggac
    15061 ttcttccccg ccggcgactg cttccgcaag cagtacgagg accagctgag ctgatctagt
    15121 tctagagtcg acccgggcgg ccgcttccct ttagtgaggg ttaatgcttc gagcagacat
    15181 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    15241 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    15301 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
    15361 tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc cgataaggat cgatccgggc
    15421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
    15481 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    15541 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    15601 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    15661 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    15721 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    15781 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    15841 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    15901 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
    15961 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
    16021 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
    16081 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    16141 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    16201 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    16261 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    16321 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    16381 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttgattc ttctgacaca
    16441 acagtctcga acttaaggct agagccacca tgattgaaca agatggattg cacgcaggtt
    16501 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
    16561 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
    16621 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
    16681 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
    16741 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
    16801 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
    16861 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
    16921 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
    16981 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
    17041 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
    17101 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
    17161 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
    17221 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
    17281 cgaaatgacc gaccaagcga cgcccaacct gccatcacga tggccgcaat aaaatatctt
    17341 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagcgata aggatccgcg
    17401 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
    17461 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
    17521 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
    17581 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    17641 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    17701 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    17761 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    17821 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    17881 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    17941 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    18001 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    18061 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    18121 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    18181 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    18241 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    18301 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    18361 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    18421 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    18481 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    18541 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    18601 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    18661 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    18721 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    18781 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    18841 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    18901 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    18961 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    19021 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    19081 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    19141 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    19201 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    19261 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    19321 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    19381 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    19441 ccttttgctg gccttttgct cacatggctc gacagatctt caatattggc cattagccat
    19501 attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
    19561 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca
    19621 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    19681 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    19741 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    19801 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    19861 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    19921 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
    19981 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
    20041 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    20101 ccaaaatcaa cgggactttc caaaatgtcg taacaactgc gatcgcccgc cccgttgacg
    20161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
    20221 cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc taacgcagtc
    20281 agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa ggtagccttg
    20341 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
    20401 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
    20461 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
    20521 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc tcgagaattc
    20581 acgcgtgccg cc
//
