LOCUS       sequence-331054-         336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of yeast Ash1 truncation (encodes amino acids 
            420-500).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Martin EW, Holehouse AS, Grace CR, Hughes A, Pappu RV, Mittag T
  TITLE     Sequence Determinants of the Conformational Properties of an 
            Intrinsically Disordered Protein Prior to and upon Multisite 
            Phosphorylation.
  JOURNAL   J Am Chem Soc. 2016 Nov 30;138(47):15323-15335. doi: 
            10.1021/jacs.6b10272. Epub 2016 Nov 17.
  PUBMED    27807972
REFERENCE   2  (bases 1 to 336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.6b10272"; journalName: "J Am Chem Soc"; date: 
            "2016-11-30- 30"; volume: "138"; issue: "47"; pages: "15323-15335"
COMMENT     Sequence Label: Ash1
FEATURES             Location/Qualifiers
     source          1..336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(250..270)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(273..297)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(307..324)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcacacgcac actcttgtgg tgtgacgagt gggtgatgag ccactggacc ttctacttcc
       61 ctttttcgtt atgcttgaat ttgatgatct tcgagggctt tgagggggag agtcgagagc
      121 aaatctatga taattgggtg accttgggct tggagtgtat gccttgggac gcacaggtga
      181 ggatgatctt gatctcatct tgccactttt cgtcggtgtg gagggagatg gagatgaaga
      241 tgcagaggcg ccctgaaaat acaggttttc gaagcctgct tttttgtaca aacttgttga
      301 ttcgaggtga tggtgatggt gatggtagta cgacat
//
