LOCUS       sequence-331057-        3724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-359d-3X
FEATURES             Location/Qualifiers
     source          1..3724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(714..731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(714..730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(723..745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(939..958)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1058..1080
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1118..1136)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1204..1308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1309..1377,1378..2169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1527..1546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2340..2928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2829..2848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3082..3099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3216..3237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3252..3257,3258..3275,3276..3282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3290..3306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3295..3317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3314..3330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3314..3330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg
       61 gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
      121 aattgagcgg cctcggcacc gggatgcgtt ttttttttca tactcgagca tgcttttttt
      181 tttcatgtgc cggtctcaca cgtgcctgga gactagtaag cgcttctagt ggcggttaaa
      241 acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga
      301 gcggcctcgg caccgggatg cgtttttttt ttcgtttgca gcggtaccgc tttggacgtt
      361 ttttttttca tgtgccggtc tcacacgtgc ctggagacta gtaagcgctt ctagtggcgg
      421 ttaaaacgcg gtagacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
      481 attgagcggc ctcggcaccg ggatgcgttt tttttttcat actcgagcat gctttttttt
      541 ttcatgtgcc ggtctcacac gtgcctggag actagtaagc gcttctagtg gcggttaaaa
      601 cgcggtagac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
      661 cggcctcggc accgggatgc gttttttttt tcgtttgcag cggtaccgaa ttcactggcc
      721 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      781 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      841 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
      901 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      961 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1021 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1081 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     1141 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1201 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1261 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1321 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1381 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1441 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1501 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1561 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1621 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1681 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1741 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1801 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1861 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1921 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1981 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2041 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2101 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2161 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2221 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2281 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2341 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2401 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2461 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2521 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2581 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2641 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2701 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2761 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2821 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2881 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2941 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3001 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3061 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3121 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3181 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3241 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     3301 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg taccgctttg
     3361 gacgtttttt ttttcatgtg ccggtctcac acgtgcctgg agactagtaa gcgcttctag
     3421 tggcggttaa aacgcggtag acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     3481 cgaccaattg agcggcctcg gcaccgggat gcgttttttt tttcatactc gagcatgctt
     3541 ttttttttca tgtgccggtc tcacacgtgc ctggagacta gtaagcgctt ctagtggcgg
     3601 ttaaaacgcg gtagacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     3661 attgagcggc ctcggcaccg ggatgcgttt tttttttcgt ttgcagcggt accgctttgg
     3721 acgt
//
