LOCUS       sequence-331061-       17548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 17548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU205
FEATURES             Location/Qualifiers
     source          1..17548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7710..7730
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7755..11672
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             11673..11720
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    12232..12356
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12381..12386,12387..12404,12405..12411)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             12465..13145
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(12531..12550)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             13465..13770
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     13649..13668
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(13811..13935)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(16189..16191,16192..16194,16195..16908)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(16189..16191,16192..16194,16195..16905)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(16234..16255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(16495..16514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16842..16863
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(16907..17157)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17168..17185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17168..17184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17177..17199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    17380..17404
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcaaaaatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
     6781 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
     6841 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
     6901 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
     6961 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
     7021 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
     7081 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
     7141 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
     7201 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
     7261 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
     7321 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
     7381 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
     7441 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
     7501 ctgttatatc atttttttta ttaattgtgt atatatatat gtgcatagat ctggattaca
     7561 tgattgtgat tatttacatg attttgttat ttacgtatgt atatatgtag atctggactt
     7621 tttggagttg ttgacttgat tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg
     7681 ttatgtatgt gcagcgaatt cggcgcgccc ctaagaagaa gcggaaggtt ggtattcacg
     7741 gggtgcctgc ggctacgcag ttcgaggggt tcaccaacct ctaccaggtc agcaagacgc
     7801 tgcggttcga gctgattccg cagggcaaga ccctcaagca catccaggag cagggcttta
     7861 tcgaggagga caaagcgcgg aacgaccact acaaggagct caagccgatc atcgaccgga
     7921 tctacaagac ctacgcggac cagtgcctgc agctcgtgca gctcgactgg gagaacctct
     7981 cggccgccat cgactcctac cgcaaggaga agacggagga gacacgcaac gcgctcatcg
     8041 aagaacaggc tacgtatcgc aacgctatcc acgactactt catcgggcgc acagataatc
     8101 tcaccgacgc catcaacaag cgccacgccg aaatctataa ggggctgttt aaggccgagc
     8161 tgttcaatgg caaagtgctg aagcaactgg gcaccgtcac gaccacagag catgagaacg
     8221 ccctcctccg gtcgttcgac aagtttacga catactttag cggcttttac gagaaccgca
     8281 aaaacgtgtt cagcgccgag gacattagca ccgccatccc gcataggatc gtgcaagaca
     8341 acttcccgaa gttcaaggag aactgccaca tcttcacccg cctcatcacg gccgtgccgt
     8401 ccctccgcga gcacttcgag aacgtgaaga aggccatcgg gatcttcgtg tccacctcga
     8461 tcgaagaggt gttctccttc cccttctaca atcagctgct gacgcagacc cagatcgacc
     8521 tctacaacca gctcctcggc ggcatctccc gcgaggccgg caccgagaag atcaagggcc
     8581 tgaacgaggt gctcaatctc gccatccaga agaacgacga aaccgcgcac atcattgcct
     8641 cactcccaca taggtttatc cccctgttta agcagatcct ctccgaccgc aacacgttgt
     8701 ccttcatcct cgaggagttc aagagcgacg aggaggtcat ccagtccttc tgcaagtaca
     8761 agaccctcct ccgcaacgag aatgtgctcg aaaccgccga ggcgctgttc aatgagctca
     8821 atagcatcga tctcacccac atcttcatct cccataagaa gctggaaacc atctccagcg
     8881 ccctgtgcga ccactgggac accctccgca acgccctcta cgagcggcgc atcagcgagc
     8941 tcaccggcaa gatcacgaag tcggcgaaag agaaagtgca aaggagcctc aagcacgagg
     9001 acattaacct gcaggagatc atctccgccg cgggcaagga gctgtccgag gctttcaagc
     9061 agaagacctc ggagatcctc tctcacgccc acgcggccct ggaccagccg ctgccgacga
     9121 ccctgaaaaa gcaagaggaa aaggaaatcc tcaagtccca gctggacagc ctcctggggc
     9181 tgtaccacct tctcgactgg ttcgccgtgg acgagtccaa cgaggtcgat ccggagttta
     9241 gcgcccgcct caccgggatc aagcttgaga tggaacctag cttgagcttc tataataagg
     9301 cgcgcaatta cgcgaccaag aagccgtatt ccgtggagaa gttcaagctg aacttccaaa
     9361 tgcccaccct ggccagcggg tgggacgtta acaaagagaa gaacaacgga gccattcttt
     9421 tcgtgaaaaa tgggttgtat tatttgggaa ttatgccgaa acaaaaaggc aggtacaagg
     9481 cgctcagctt cgagccaact gagaagacct ccgagggctt cgacaagatg tactacgatt
     9541 attttcctga cgctgcaaag atgataccga agtgcagcac tcagcttaag gcggtgacgg
     9601 cgcactttca gacccatacc acccccatcc tcctctccaa caacttcatc gagccgctcg
     9661 agatcaccaa ggagatatac gatctgaata atccagaaaa ggaacccaag aagttccaga
     9721 ccgcctacgc caagaagacg ggcgatcaaa aggggtatag agaggcgctc tgcaagtgga
     9781 tcgacttcac gcgcgatttc ctcagcaagt acaccaagac aacctccatc gatctctctt
     9841 ccctccgccc ctcttcccag tacaaggacc tcggggagta ctacgccgaa ctcaacccac
     9901 tcctgtatca catctcgttt cagcgtatcg cggaaaagga gatcatggac gccgtcgaaa
     9961 ccggcaagtt gtatcttttc caaatctata acaaggactt cgcgaagggc caccacggga
    10021 agccaaacct gcacaccctc tactggacag gcctcttcag cccggaaaat ctcgcgaaga
    10081 cgagcataaa gctgaacggc caggcagaac tcttctacag gccgaagtcc aggatgaagc
    10141 gcatggctca tcgcctcggt gagaagatgc tgaacaagaa gctgaaagat caaaagacgc
    10201 caatccctga tacactgtat caggagctgt acgattacgt gaaccaccgc ctctcacacg
    10261 acctcagcga cgaggcccgc gcgctcctgc caaacgtcat cacgaaggag gtcagccacg
    10321 agatcataaa ggatcggcgg tttacctctg acaagttctt tttccatgtc cccatcacgc
    10381 tgaactacca ggccgcgaat agcccgtcca agttcaacca gcgggtcaac gcgtatctca
    10441 aggagcaccc agagacaccc ataatcggga ttgaccgggg ggagcggaac ctcatctaca
    10501 tcaccgtcat cgactccacc ggaaagattc tcgagcaacg gtcgctcaat accatccagc
    10561 agttcgacta ccagaagaag ctcgacaacc gggagaagga acgcgtcgcc gcgaggcagg
    10621 cctggtccgt agtgggcacg atcaaagacc tgaagcaggg ctatctcagc caggtcatcc
    10681 atgagatagt ggatctcatg atccactacc aagccgtcgt ggtcctcgag aatctcaatt
    10741 tcggattcaa atccaagcgc acaggcatcg ccgagaaggc ggtgtaccaa cagttcgaga
    10801 aaatgcttat cgacaagctc aattgcctgg tgctcaagga ctatccggcg gagaaggtcg
    10861 ggggggtcct caatccgtat cagctgaccg accagtttac gtcatttgcg aagatgggca
    10921 cccagagcgg cttccttttc tatgtcccgg ccccatatac ctcaaagatt gatcccttga
    10981 ccggattcgt ggacccgttt gtctggaaga ccatcaagaa ccatgagtcg cgtaagcatt
    11041 tcctggaggg tttcgacttc ctgcactatg atgtaaaaac cggagacttc atcctgcatt
    11101 tcaagatgaa tcggaacctc tccttccagc ggggactccc tggcttcatg cccgcttggg
    11161 atatcgtttt tgagaaaaat gaaacccaat tcgacgccaa aggcacgcct ttcatcgcgg
    11221 gcaagaggat tgtccctgta attgagaacc atagattcac cgggcgttac cgtgacctgt
    11281 accccgcaaa cgaactcatc gccctcctgg aggagaaagg catcgttttc cgcgacgggt
    11341 caaatatcct ccccaaactg ctcgagaacg atgatagcca cgctattgac acgatggtag
    11401 cgctcatcag atccgtgctg caaatgagaa attcaaatgc tgccactggg gaggattaca
    11461 tcaactcccc tgtgcgtgat ctcaatggcg tgtgcttcga ttctagattt cagaatcctg
    11521 agtggccgat ggatgccgat gctaacggcg cataccacat agcattgaaa ggacaactgt
    11581 tgttgaacca tctcaaggag agcaaggacc ttaagctgca gaacggcatc agcaaccagg
    11641 attggcttgc ctatatccaa gagctccgca ataagcgtcc tgctgccacc aaaaaggccg
    11701 gacaggctaa gaaaaagaag tgaggcgcgc cgagctccag gcctcccagc tttcgtccgt
    11761 atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc ccacacacca
    11821 gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct atcatttgtt
    11881 ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa tgcaaatgga
    11941 tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat tattatctgt
    12001 tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt gatattaagg
    12061 tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct tgaattggtt
    12121 tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa gtaattatgc
    12181 aatcaagtta agtacagtat aggcttgagc tccctaggcc tgttatccct aacaagtttg
    12241 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt
    12301 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg
    12361 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
    12421 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
    12481 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
    12541 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
    12601 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
    12661 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
    12721 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
    12781 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
    12841 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
    12901 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
    12961 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
    13021 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
    13081 atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag gatccggctt
    13141 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
    13201 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
    13261 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
    13321 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
    13381 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
    13441 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
    13501 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
    13561 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
    13621 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
    13681 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
    13741 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
    13801 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
    13861 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
    13921 cttgtacaaa gtggttcgat aattccgatc cagcctaggc ccgggcctga ggacgcgtcc
    13981 atggttaatt aagacgtccg gaccgactag tggatcctct agagtcgacc tgcaggcatg
    14041 caagcttctt caagtgtatg taggtatagt aacatgaaca agaacagact caagtacaag
    14101 atcgcatacg aaaatggaaa tggcaatgtc acttccacat aatcaaacac gaatcctcat
    14161 atcaacaagg cctgagattc taactagctc ataacaactt agccaatagt tacttgagac
    14221 taccaaatgt atgtagaact aaagactaag ggacagagag ttcgtctaaa caggtgaatc
    14281 tagtcgttgt tatctaataa acaattcagc cccaaatgca gaacacacat agagctctct
    14341 attgattcaa attacgatct gatactgata acgtctagat ttttagggtt aaagcaatca
    14401 atcacctgac gattcaaggt ggttggatca tgacgattcc agaaaacatc aagcaagctc
    14461 tcaaagctac actctttggg atcatactga actctaacaa cctcgttatg tcccgtagtg
    14521 ccagtacaga catcctcgta actcggattg tgcacgatgc catggctata cccaacctcg
    14581 gtcttggtca caccaggaac tctctggtaa gctagctcca ctccccagaa acaaccggcg
    14641 ccaaattgcg cgaattgctg acctgaagac ggaacatcat cgtcgggtcc ttgggcgatt
    14701 gcggcggaag atgggtcagc ttgggcttga ggacgagacc cgaatccgag tctgttgaaa
    14761 aggttgttca ttggggattt gtatacggag attggtcgtc gagaggtttg agggaaagga
    14821 caaatgggtt tggctctgga gaaagagagt gcggctttag agagagaatt gagaggttta
    14881 gagagagatg cggcggcgat gagcggagga gagacgacga ggacctgcat tatcaaagca
    14941 gtgacgtggt gaaatttgga acttttaaga ggcagataga tttattattt gtatccattt
    15001 tcttcattgt tctagaatgt cgcggaacaa attttaaaac taaatcctaa atttttctaa
    15061 ttttgttgcc aatagtggat atgtgggccg tatagaagga atctattgaa ggcccaaacc
    15121 catactgacg agcccaaagg ttcgttttgc gttttatgtt tcggttcgat gccaacgcca
    15181 cattctgagc taggcaaaaa acaaacgtgt ctttgaatag actcctctcg ttaacacatg
    15241 cagcggctgc atggtgacgc cattaacacg tggcctacaa ttgcatgatg tctccattga
    15301 cacgtgactt ctcgtctcct ttcttaatat atctaacaaa cactcctacc tcttccaaaa
    15361 tatatacaca tctttttgat caatctctca ttcaaaatct cattctctct agtaaacaag
    15421 aacaaaaaaa tggcggatac agctagagga acccatcacg atatcatcgg cagagaccag
    15481 tacccgatga tgggccgaga ccgagaccag taccagatgt ccggacgagg atctgactac
    15541 tccaagtcta ggcagattgc taaagctgca actgctgtca cagctggtgg ttccctcctt
    15601 gttctctcca gccttaccct tgttggaact gtcatagctt tgactgttgc aacacctctg
    15661 ctcgttatct tcagcccaat ccttgtcccg gctctcatca cagttgcact cctcatcacc
    15721 ggttttcttt cctctggagg gtttggcatt gccgctataa ccgttttctc ttggatttac
    15781 aagtaagcac acatttatca tcttacttca taattttgtg caatatgtgc atgcatgtgt
    15841 tgagccagta gctttggatc aatttttttg gtcgaataac aaatgtaaca ataagaaatt
    15901 gcaaattcta gggaacattt ggttaactaa atacgaaatt tgacctagct agcttgaatg
    15961 tgtctgtgta tatcatctat ataggtaaaa tgcttggtat gatacctatt gattgtgaat
    16021 aggtacgcaa cgggagagca cccacaggga tcagacaagt tggacagtgc aaggatgaag
    16081 ttgggaagca aagctcagga tctgaaagac agagctcagt actacggaca gcaacatact
    16141 ggtggggaac atgaccgtga ccgtactcgt ggtggccagc acactactat ggtgagcaag
    16201 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
    16261 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
    16321 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
    16381 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
    16441 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
    16501 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
    16561 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
    16621 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
    16681 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
    16741 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
    16801 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
    16861 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgatc tagtaacata
    16921 gatgaaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
    16981 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
    17041 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
    17101 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatgaa
    17161 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    17221 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    17281 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgagcttga gcttggatca
    17341 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
    17401 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
    17461 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
    17521 tactttaaag tactttaaag tactttaa
//
