LOCUS       sequence-331062-        3711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-345c-3X
FEATURES             Location/Qualifiers
     source          1..3711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(129..146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(129..145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(138..160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(354..373)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     473..495
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(533..551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        619..723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(724..792,793..1584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(942..961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1755..2343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2244..2263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2497..2514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2631..2652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2667..2672,2673..2690,2691..2697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2705..2721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2710..2732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2729..2745
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2729..2745
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga
       61 gcggcctcgg caccgggatg cgtttttttt tcagcggtac cggatccccg ggtaccgagc
      121 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      181 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      241 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      301 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      361 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      421 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      481 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2581 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2641 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2701 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2761 cttggagatc tggtaccgct gttttttttt catgtgccgg tctcacacgt gcctggagac
     2821 tagtaagcgc ttctagtggc ggttaaaacg cggtagacag cgcgtacgtg cgtttaagcg
     2881 gtgctagagc tgtctacgac caattgagcg gcctcggcac cgggatgcgt tttttttttc
     2941 atactcgagc atgctttttt ttttcatgtg ccggtctcac acgtgcctgg agactagtaa
     3001 gcgcttctag tggcggttaa aacgcggtag acagcgcgta cgtgcgttta agcggtgcta
     3061 gagctgtcta cgaccaattg agcggcctcg gcaccgggat gcgttttttt ttcagcggta
     3121 ccgctgtttt tttttcatgt gccggtctca cacgtgcctg gagactagta agcgcttcta
     3181 gtggcggtta aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct agagctgtct
     3241 acgaccaatt gagcggcctc ggcaccggga tgcgtttttt ttttcatact cgagcatgct
     3301 tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg
     3361 gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
     3421 aattgagcgg cctcggcacc gggatgcgtt tttttttcag cggtaccgct gttttttttt
     3481 catgtgccgg tctcacacgt gcctggagac tagtaagcgc ttctagtggc ggttaaaacg
     3541 cggtagacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg
     3601 gcctcggcac cgggatgcgt tttttttttc atactcgagc atgctttttt ttttcatgtg
     3661 ccggtctcac acgtgcctgg agactagtaa gcgcttctag tggcggttaa a
//
