LOCUS       sequence-331066-        1883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, 
            I217K, Y353E).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, 
            Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA,
            Mittag T
  TITLE     Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation 
            of Active, Phase-Separated Compartments.
  JOURNAL   Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 
            10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20.
  PUBMED    30244836
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08.027"; journalName: "Mol Cell"; date: 
            "2018-10-4- 4"; volume: "72"; issue: "1"; pages: "19-36.e8"
COMMENT     Sequence Label: SPOP-V5 (BTB+BACK)
FEATURES             Location/Qualifiers
     source          1..1883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     100..119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        100..118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             241..282
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1439..1456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1445..1669
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1802..1821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
       61 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      121 gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
      181 gtgtggtgga attctgcaga tatcatgggt aagcctatcc cgaattctgc agatatcatg
      241 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgatgtcaag ggttccaagt
      301 cctccacctc cggcagaaat gtcgagtggt cccgttgccg agagctggtg ctatacacag
      361 atcaaggtag tgaagttctc ctacatgtgg accatcaata actttagttt ttgccgggag
      421 gaaatgggtg aagtcataaa aagttcaact ttttcatcag gagccaatga taaactgaaa
      481 tggtgtttgc gagtaaaccc gaaagggcta gatgaagaaa gcaaagatta cctgtcgctt
      541 tacctgttgt tggtcagctg tccaaagagt gaagttcggg caaagttcaa gttctccatc
      601 ctgaatgcca agggagaaga aaccaaagct atggagagtc agcgagctta taggttcgtg
      661 caaggcaaag actggggatt caaaaaattc atccgcagag attttctctt ggacgaggcc
      721 aatgggcttc tccctgatga caagctcacc ctcttctgtg aggtgagtgt agtacaagac
      781 tctgtcaata tttctgggca gaatactatg aacatggtaa aggttcctga atgccgggac
      841 gcagatgagg acggagggga ttgggagaac tctcgattca ccgactgctg cttgtgtgtg
      901 gctggtcaag aattccaggc tcacaaggct aagttagcag ctcgttctcc agtcttcagt
      961 gccatgtttg aacatgaaat ggaggagagc aaaaagaatc gggttgagat taacgacgtg
     1021 gagcctgagg tgtttaagga gatgatgtgc ttcatctaca cggggaaggc gccgaacctc
     1081 gacaagatgg ctgacgactt gctggcagct gccgataagt acgccctgga gcgtctgaag
     1141 gtcatgtgtg aggacgccct ctgcagtaac ctctctgtgg agaacgctgc agagattctc
     1201 atcctggctg acctccacag cgcagatcag ctgaagacgc aggcagttga tttcatcaac
     1261 tatcatgctt cggatgtctt ggagacctct gggtggaagt caatggtggt gtcacatccc
     1321 cacttagtgg cagaagcaga gcgttctctg gcttcagcac agtgcccttt cctggggccc
     1381 ccacgcaaac gcctgaagca atcctaatct agagggcccg tttaaacccg ctgatcagcc
     1441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     1501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1561 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1621 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     1681 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     1741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1861 ctaaatcggg ggctcccttt agg
//
