LOCUS       sequence-331068-        3451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-175a-4X
FEATURES             Location/Qualifiers
     source          1..3451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(338..355)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(338..354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(347..369)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(563..582)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     682..704
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(742..760)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        828..932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(933..1001,1002..1793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1151..1170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1964..2552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2453..2472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2706..2723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2840..2861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2876..2881,2882..2899,2900..2906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2914..2930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2919..2941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2938..2954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2938..2954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcttaaacgc acgtacggaa tcagatccgg taccctgctt ttttttttca caatcccggt
       61 gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacggatt
      121 cggatctggt accctgcttt tttttttcac aatcccggtg ccgaggccgc tcaattggtc
      181 gtagacagct ctagcaccgc ttaaacgcac gtacggaatc agatccggta ccctgctttt
      241 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
      301 taaacgcacg tacggattcg gatccggtac cgaattcact ggccgtcgtt ttacaacgtc
      361 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      421 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
      481 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
      541 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      601 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      661 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
      721 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      781 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1141 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1441 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2881 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2941 gaaacagcta tgaccatgat tacgccaagc ttaaaaaaag atctggtacc ctgctttttt
     3001 ttttcacaat cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta
     3061 aacgcacgta cggaatcaga tccggtaccc tgcttttttt tttcacaatc ccggtgccga
     3121 ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac ggattcggat
     3181 ctggtaccct gctttttttt ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga
     3241 cagctctagc accgcttaaa cgcacgtacg gaatcagatc cggtaccctg cttttttttt
     3301 tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac
     3361 gcacgtacgg attcggatct ggtaccctgc tttttttttt cacaatcccg gtgccgaggc
     3421 cgctcaattg gtcgtagaca gctctagcac c
//
