LOCUS       sequence-331073-         480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of human hnRNPA1-A Low complexity domain 
            including amino acids 186-320, hexapeptide including aa 259-264 
            deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, 
            Grace CR, Soranno A, Pappu RV, Mittag T
  TITLE     Valence and patterning of aromatic residues determine the phase 
            behavior of prion-like domains.
  JOURNAL   Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653.
  PUBMED    32029630
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-02-7- 7"; volume: "367"; issue: 
            "6478"; pages: "694-699"
COMMENT     Sequence Label: A1-LCD deltahexa
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaaa acctgtattt tcagggcagc atggctagtg cttcatccag ccaaagaggt
      121 cgaagtggtt ctggaaactt tggtggtggt cgtggaggtg gtttcggtgg gaatgacaac
      181 ttcggtcgtg gaggaaactt cagtggtcgt ggtggctttg gtggcagccg tggtggtggt
      241 ggatatggtg gcagtgggga tggctataat ggatttggta atgatggaag caattttgga
      301 ggtggtggaa attacaacaa tcagtcttca aattttggac ccatgaaggg aggaaatttt
      361 ggaggcagaa gctctggccc ctatggcggt ggaggccaat actttgcaaa accacgaaac
      421 caaggtggct atggcggttc cagcagcagc agtagctatg gcagtggcag aagattttaa
//
