LOCUS       sequence-331074-        5674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-167a-16X
FEATURES             Location/Qualifiers
     source          1..5674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3042..3058)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3042..3058)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3055..3077)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3066..3082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3090..3096,3097..3114,3115..3120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3135..3156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3273..3290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3444..4032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3524..3543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4203..4994,4995..5063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4826..4845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5064..5168)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5236..5254
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5292..5314)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5414..5433
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5627..5649
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5641..5658
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5642..5658
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cccggggatc ctgcagtttt ttttttcaca atcccggtgc cgaggccgct caattggtcg
       61 tagacagctc tagcaccgct taaacgcacg tacggaatca gatcctgcag tttttttttt
      121 cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg
      181 cacgtacgga ttcagatcct gcagtttttt ttttcacaat cccggtgccg aggccgctca
      241 attggtcgta gacagctcta gcaccgctta aacgcacgta cggaatcaga tcctgcagtt
      301 ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg
      361 cttaaacgca cgtacggatt cagatcctgc agtttttttt ttcacaatcc cggtgccgag
      421 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gaatcagatc
      481 ctgcagtttt ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc
      541 tagcaccgct taaacgcacg tacggattca gatcctgcag tttttttttt cacaatcccg
      601 gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgga
      661 atcagatcct gcagtttttt ttttcacaat cccggtgccg aggccgctca attggtcgta
      721 gacagctcta gcaccgctta aacgcacgta cggattcaga tcctgcagtt ttttttttca
      781 caatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca
      841 cgtacggaat cagatcctgc agtttttttt ttcacaatcc cggtgccgag gccgctcaat
      901 tggtcgtaga cagctctagc accgcttaaa cgcacgtacg gattcagatc ctgcagtttt
      961 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
     1021 taaacgcacg tacggaatca gatcctgcag tttttttttt cacaatcccg gtgccgaggc
     1081 cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgga ttcagatcct
     1141 gcagtttttt ttttcacaat cccggtgccg aggccgctca attggtcgta gacagctcta
     1201 gcaccgctta aacgcacgta cggaatcaga tcctgcagtt ttttttttca caatcccggt
     1261 gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacggatt
     1321 cagatcctgc agtttttttt ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga
     1381 cagctctagc accgcttaaa cgcacgtacg gaatcagatc ctgcagtttt ttttttcaca
     1441 atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg
     1501 tacggattca gatcctgcag tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     1561 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagtttttt
     1621 ttttcacaat cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta
     1681 aacgcacgta cggattcaga tcctgcagtt ttttttttca caatcccggt gccgaggccg
     1741 ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacggaat cagatcctgc
     1801 agtttttttt ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc
     1861 accgcttaaa cgcacgtacg gattcagatc ctgcagtttt ttttttcaca atcccggtgc
     1921 cgaggccgct caattggtcg tagacagctc tagcaccgct taaacgcacg tacggaatca
     1981 gatcctgcag tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca
     2041 gctctagcac cgcttaaacg cacgtacgga ttcagatcct gcagtttttt ttttcacaat
     2101 cccggtgccg aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta
     2161 cggaatcaga tcctgcagtt ttttttttca caatcccggt gccgaggccg ctcaattggt
     2221 cgtagacagc tctagcaccg cttaaacgca cgtacggatt cagatcctgc agtttttttt
     2281 ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     2341 cgcacgtacg gaatcagatc ctgcagtttt ttttttcaca atcccggtgc cgaggccgct
     2401 caattggtcg tagacagctc tagcaccgct taaacgcacg tacggattca gatcctgcag
     2461 tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
     2521 cgcttaaacg cacgtacgga atcagatcct gcagtttttt ttttcacaat cccggtgccg
     2581 aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta cggattcaga
     2641 tcctgcagtt ttttttttca caatcccggt gccgaggccg ctcaattggt cgtagacagc
     2701 tctagcaccg cttaaacgca cgtacggaat cagatcctgc agtttttttt ttcacaatcc
     2761 cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg
     2821 gattcagatc ctgcagtttt ttttttcaca atcccggtgc cgaggccgct caattggtcg
     2881 tagacagctc tagcaccgct taaacgcacg tacggaatca gatcctgcag tttttttttt
     2941 cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac cgcttaaacg
     3001 cacgtacgga ttcagatctg cagaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     3061 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3181 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3241 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3301 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3361 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3421 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4021 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4621 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5161 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     5221 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     5281 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     5341 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     5401 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     5461 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
     5521 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     5581 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     5641 tgtaaaacga cggccagtga attcgagctc ggta
//
