LOCUS       sequence-331077-        3395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-nonpalindromic-169an-4X
FEATURES             Location/Qualifiers
     source          1..3395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(369..386)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(369..385)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..400)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(594..613)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     713..735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(773..791)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        859..963
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(964..1032,1033..1824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1182..1201)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1995..2583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2484..2503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2737..2754
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2871..2892
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2907..2912,2913..2930,2931..2937)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2945..2961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2950..2972
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2969..2985
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2969..2985
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaaaa cagcatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
       61 cgcttaaacg cacgtacgcg ctgtctaccg cgttttaacc gccactagaa gcgcttacta
      121 gtctccaggc acgtgtgaga ccggcacatg caaaaaaaaa acgagctcgc aggagcgcgg
      181 tacccaaaaa aaaaacagca tcccggtgcc gaggccgctc aattggtcgt agacagctct
      241 agcaccgctt aaacgcacgt acgcgctgtc taccgcgttt taaccgccac tagaagcgct
      301 tactagtctc caggcacgtg tgagaccggc acatgcaaaa aaaaaacgag ctcaaaggat
      361 ccgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      421 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      481 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      541 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      601 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      661 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      721 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      781 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      841 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      901 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      961 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1021 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1081 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1141 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1201 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1261 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1321 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1381 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1441 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1501 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1561 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1621 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1681 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1741 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1801 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1861 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1921 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1981 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2041 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2101 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2161 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2221 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2281 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2341 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2401 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2461 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2521 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2581 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2641 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2701 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2761 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2821 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2881 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2941 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     3001 cttaaaaaaa gatctaaagg tacccaaaaa aaaaacagca tcccggtgcc gaggccgctc
     3061 aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc taccgcgttt
     3121 taaccgccac tagaagcgct tactagtctc caggcacgtg tgagaccggc acatgcaaaa
     3181 aaaaaacgag ctcgcaggag cgcggtaccc aaaaaaaaaa cagcatcccg gtgccgaggc
     3241 cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgcg ctgtctaccg
     3301 cgttttaacc gccactagaa gcgcttacta gtctccaggc acgtgtgaga ccggcacatg
     3361 caaaaaaaaa acgagctcgc aggagcgcgg taccc
//
