LOCUS       sequence-331079-         499 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of human hnRNPA1-A Low complexity domain 
            including amino acids 186-320, aromatic amino acids. TYR and PHE 
            shuffled into 5 clusters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, 
            Grace CR, Soranno A, Pappu RV, Mittag T
  TITLE     Valence and patterning of aromatic residues determine the phase 
            behavior of prion-like domains.
  JOURNAL   Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653.
  PUBMED    32029630
REFERENCE   2  (bases 1 to 499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-02-7- 7"; volume: "367"; issue: 
            "6478"; pages: "694-699"
COMMENT     Sequence Label: A1-LCD Perfect Mix
FEATURES             Location/Qualifiers
     source          1..499
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(413..433)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(436..460)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(470..487)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttaacgacg accgctaaaa cctgagcttg agctgctaaa gcttccacca ccaccttgat
       61 aattacgcgg ttttgcctga aaaccaccgc cacctgaacc aaaaccgctg ctacgaccgc
      121 cataattacc acctttcatc ggaaagccat tgctgctctg attatagtta ttgccatcat
      181 ttgaaaatcc gccaccgcca ttgctaaaac catcattacc gccattataa ccatcaccac
      241 taccgccaaa accacctccg ccacggctat aaccgccacc accgcgacca aagctattac
      301 caccacggcc aaaattatca ttgccaccac cataaccacc acgaccacca ccaaaattac
      361 cgctaccgct acgataacca cgctggctgc tgctaaatgc gcttgccatg ctaccctgaa
      421 aatacaggtt ttcgaagcct gcttttttgt acaaacttgt tgattcgagg tgatggtgat
      481 ggtgatggta gtacgacat
//
