LOCUS       sequence-331089-       17915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17915)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 17915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU55
FEATURES             Location/Qualifiers
     source          1..17915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8101..8121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8146..12063
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             12064..12111
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    12623..12747
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12772..12777,12778..12795,12796..12802)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             12856..13536
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(12922..12941)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             13856..14161
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     14040..14059
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(14202..14326)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             16580..17275
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(17274..17525)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17535..17552)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17535..17551)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17544..17566)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    17747..17771
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcggtaccg aataaaagca tttgcgtttg gtttatcatt gcgtttatac aaggacagag
     6781 atccactgag ctggaatagc ttaaaaccat tatcagaaca aaataaacca ttttttgtta
     6841 agaatcagag catagtaaac aacagaaaca acctaagaga ggtaacttgt ccaagaagat
     6901 agctaattat atctatttta taaaagttat catagtttgt aagtcacaaa agatgcaaat
     6961 aacagagaaa ctaggagact tgagaatata cattcttgta tatttgtatt cgagattgtg
     7021 aaaatttgac cataagttta aattcttaaa aagatatatc tgatctaggt gatggttata
     7081 gactgtaatt ttaccacatg tttaatgatg gatagtgaca cacatgacac atcgacaaca
     7141 ctatagcatc ttatttagat tacaacatga aatttttctg taatacatgt ctttgtacat
     7201 aatttaaaag taattcctaa gaaatatatt tatacaagga gtttaaagaa aacatagcat
     7261 aaagttcaat gagtagtaaa aaccatatac agtatatagc ataaagttca atgagtttat
     7321 tacaaaagca ttggttcact ttctgtaaca cgacgttaaa ccttcgtctc caataggagc
     7381 gctactgatt caacatgcca atatatacta aatacgtttc tacagtcaaa tgctttaacg
     7441 tttcatgatt aagtgactat ttaccgtcaa tcctttccca ttcctcccac taatccaact
     7501 ttttaattac tcttaaatca ccactaagct agtaacgcct atcatgaatt agctctacta
     7561 aatctagcaa cctttcaaat ttgcagtatt gcaggtgtct ctgtgtcttt aaaatagttg
     7621 ccttatgatt tcttcggttt caagatgatc aaatagttat agatttcatg ctcacacatg
     7681 ctcattagat gtgtacatac tttacttacc caaatctatt ttctcgcaaa gattttgatg
     7741 gtaaagctga tttggttcta ttgaactaaa tcaaacgagt ttcagactga gtgattctaa
     7801 tccggcccat tagcccctaa acagacccac taattacgca gcttttaata gagtaattac
     7861 acctagttta cccactaaac cactaagcac taattatctc acaatctaat gagcttccct
     7921 cgtaattact tgggctttca ctctaccatt tatttgtaac agtcaagtct ctactgtctc
     7981 tatataaact ctctaaagtt aacacacaat tctcatcaca aacaaatcaa ccaaagcaac
     8041 ttctactctt tcttctttcg accttatcaa tctgttgaga aggtaccaat tcggcgcgcc
     8101 cctaagaaga agcggaaggt tggtattcac ggggtgcctg cggctacgca gttcgagggg
     8161 ttcaccaacc tctaccaggt cagcaagacg ctgcggttcg agctgattcc gcagggcaag
     8221 accctcaagc acatccagga gcagggcttt atcgaggagg acaaagcgcg gaacgaccac
     8281 tacaaggagc tcaagccgat catcgaccgg atctacaaga cctacgcgga ccagtgcctg
     8341 cagctcgtgc agctcgactg ggagaacctc tcggccgcca tcgactccta ccgcaaggag
     8401 aagacggagg agacacgcaa cgcgctcatc gaagaacagg ctacgtatcg caacgctatc
     8461 cacgactact tcatcgggcg cacagataat ctcaccgacg ccatcaacaa gcgccacgcc
     8521 gaaatctata aggggctgtt taaggccgag ctgttcaatg gcaaagtgct gaagcaactg
     8581 ggcaccgtca cgaccacaga gcatgagaac gccctcctcc ggtcgttcga caagtttacg
     8641 acatacttta gcggctttta cgagaaccgc aaaaacgtgt tcagcgccga ggacattagc
     8701 accgccatcc cgcataggat cgtgcaagac aacttcccga agttcaagga gaactgccac
     8761 atcttcaccc gcctcatcac ggccgtgccg tccctccgcg agcacttcga gaacgtgaag
     8821 aaggccatcg ggatcttcgt gtccacctcg atcgaagagg tgttctcctt ccccttctac
     8881 aatcagctgc tgacgcagac ccagatcgac ctctacaacc agctcctcgg cggcatctcc
     8941 cgcgaggccg gcaccgagaa gatcaagggc ctgaacgagg tgctcaatct cgccatccag
     9001 aagaacgacg aaaccgcgca catcattgcc tcactcccac ataggtttat ccccctgttt
     9061 aagcagatcc tctccgaccg caacacgttg tccttcatcc tcgaggagtt caagagcgac
     9121 gaggaggtca tccagtcctt ctgcaagtac aagaccctcc tccgcaacga gaatgtgctc
     9181 gaaaccgccg aggcgctgtt caatgagctc aatagcatcg atctcaccca catcttcatc
     9241 tcccataaga agctggaaac catctccagc gccctgtgcg accactggga caccctccgc
     9301 aacgccctct acgagcggcg catcagcgag ctcaccggca agatcacgaa gtcggcgaaa
     9361 gagaaagtgc aaaggagcct caagcacgag gacattaacc tgcaggagat catctccgcc
     9421 gcgggcaagg agctgtccga ggctttcaag cagaagacct cggagatcct ctctcacgcc
     9481 cacgcggccc tggaccagcc gctgccgacg accctgaaaa agcaagagga aaaggaaatc
     9541 ctcaagtccc agctggacag cctcctgggg ctgtaccacc ttctcgactg gttcgccgtg
     9601 gacgagtcca acgaggtcga tccggagttt agcgcccgcc tcaccgggat caagcttgag
     9661 atggaaccta gcttgagctt ctataataag gcgcgcaatt acgcgaccaa gaagccgtat
     9721 tccgtggaga agttcaagct gaacttccaa atgcccaccc tggccagcgg gtgggacgtt
     9781 aacaaagaga agaacaacgg agccattctt ttcgtgaaaa atgggttgta ttatttggga
     9841 attatgccga aacaaaaagg caggtacaag gcgctcagct tcgagccaac tgagaagacc
     9901 tccgagggct tcgacaagat gtactacgat tattttcctg acgctgcaaa gatgataccg
     9961 aagtgcagca ctcagcttaa ggcggtgacg gcgcactttc agacccatac cacccccatc
    10021 ctcctctcca acaacttcat cgagccgctc gagatcacca aggagatata cgatctgaat
    10081 aatccagaaa aggaacccaa gaagttccag accgcctacg ccaagaagac gggcgatcaa
    10141 aaggggtata gagaggcgct ctgcaagtgg atcgacttca cgcgcgattt cctcagcaag
    10201 tacaccaaga caacctccat cgatctctct tccctccgcc cctcttccca gtacaaggac
    10261 ctcggggagt actacgccga actcaaccca ctcctgtatc acatctcgtt tcagcgtatc
    10321 gcggaaaagg agatcatgga cgccgtcgaa accggcaagt tgtatctttt ccaaatctat
    10381 aacaaggact tcgcgaaggg ccaccacggg aagccaaacc tgcacaccct ctactggaca
    10441 ggcctcttca gcccggaaaa tctcgcgaag acgagcataa agctgaacgg ccaggcagaa
    10501 ctcttctaca ggccgaagtc caggatgaag cgcatggctc atcgcctcgg tgagaagatg
    10561 ctgaacaaga agctgaaaga tcaaaagacg ccaatccctg atacactgta tcaggagctg
    10621 tacgattacg tgaaccaccg cctctcacac gacctcagcg acgaggcccg cgcgctcctg
    10681 ccaaacgtca tcacgaagga ggtcagccac gagatcataa aggatcggcg gtttacctct
    10741 gacaagttct ttttccatgt ccccatcacg ctgaactacc aggccgcgaa tagcccgtcc
    10801 aagttcaacc agcgggtcaa cgcgtatctc aaggagcacc cagagacacc cataatcggg
    10861 attgaccggg gggagcggaa cctcatctac atcaccgtca tcgactccac cggaaagatt
    10921 ctcgagcaac ggtcgctcaa taccatccag cagttcgact accagaagaa gctcgacaac
    10981 cgggagaagg aacgcgtcgc cgcgaggcag gcctggtccg tagtgggcac gatcaaagac
    11041 ctgaagcagg gctatctcag ccaggtcatc catgagatag tggatctcat gatccactac
    11101 caagccgtcg tggtcctcga gaatctcaat ttcggattca aatccaagcg cacaggcatc
    11161 gccgagaagg cggtgtacca acagttcgag aaaatgctta tcgacaagct caattgcctg
    11221 gtgctcaagg actatccggc ggagaaggtc gggggggtcc tcaatccgta tcagctgacc
    11281 gaccagttta cgtcatttgc gaagatgggc acccagagcg gcttcctttt ctatgtcccg
    11341 gccccatata cctcaaagat tgatcccttg accggattcg tggacccgtt tgtctggaag
    11401 accatcaaga accatgagtc gcgtaagcat ttcctggagg gtttcgactt cctgcactat
    11461 gatgtaaaaa ccggagactt catcctgcat ttcaagatga atcggaacct ctccttccag
    11521 cggggactcc ctggcttcat gcccgcttgg gatatcgttt ttgagaaaaa tgaaacccaa
    11581 ttcgacgcca aaggcacgcc tttcatcgcg ggcaagagga ttgtccctgt aattgagaac
    11641 catagattca ccgggcgtta ccgtgacctg taccccgcaa acgaactcat cgccctcctg
    11701 gaggagaaag gcatcgtttt ccgcgacggg tcaaatatcc tccccaaact gctcgagaac
    11761 gatgatagcc acgctattga cacgatggta gcgctcatca gatccgtgct gcaaatgaga
    11821 aattcaaatg ctgccactgg ggaggattac atcaactccc ctgtgcgtga tctcaatggc
    11881 gtgtgcttcg attctagatt tcagaatcct gagtggccga tggatgccga tgctaacggc
    11941 gcataccaca tagcattgaa aggacaactg ttgttgaacc atctcaagga gagcaaggac
    12001 cttaagctgc agaacggcat cagcaaccag gattggcttg cctatatcca agagctccgc
    12061 aataagcgtc ctgctgccac caaaaaggcc ggacaggcta agaaaaagaa gtgaggcgcg
    12121 ccgagctcca ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg ttcgtcaagt
    12181 tcaatgcatc agtttcattg cccacacacc agaatcctac taagtttgag tattatggca
    12241 ttggaaaagc tgttttcttc tatcatttgt tctgcttgta atttactgtg ttctttcagt
    12301 ttttgttttc ggacatcaaa atgcaaatgg atggataaga gttaataaat gatatggtcc
    12361 ttttgttcat tctcaaatta ttattatctg ttgtttttac tttaatgggt tgaatttaag
    12421 taagaaagga actaacagtg tgatattaag gtgcaatgtt agacatataa aacagtcttt
    12481 cacctctctt tggttatgtc ttgaattggt ttgtttcttc acttatctgt gtaatcaagt
    12541 ttactatgag tctatgatca agtaattatg caatcaagtt aagtacagta taggcttgag
    12601 ctccctaggc ctgttatccc taacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
    12661 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
    12721 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
    12781 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
    12841 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
    12901 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
    12961 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
    13021 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
    13081 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
    13141 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
    13201 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
    13261 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
    13321 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
    13381 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
    13441 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
    13501 caggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt
    13561 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa
    13621 aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt
    13681 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
    13741 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc
    13801 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca
    13861 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
    13921 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
    13981 gtcagataaa gtcccccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
    14041 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
    14101 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
    14161 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt
    14221 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
    14281 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga taattccgat
    14341 ccagcctagg cccgggcctg aggacgcgtc catggttaat taagacgtcc ggaccgacta
    14401 gtggatcctc tagagtcgac ctgcaggcat gcaagcttct tcaagtgtat gtaggtatag
    14461 taacatgaac aagaacagac tcaagtacaa gatcgcatac gaaaatggaa atggcaatgt
    14521 cacttccaca taatcaaaca cgaatcctca tatcaacaag gcctgagatt ctaactagct
    14581 cataacaact tagccaatag ttacttgaga ctaccaaatg tatgtagaac taaagactaa
    14641 gggacagaga gttcgtctaa acaggtgaat ctagtcgttg ttatctaata aacaattcag
    14701 ccccaaatgc agaacacaca tagagctctc tattgattca aattacgatc tgatactgat
    14761 aacgtctaga tttttagggt taaagcaatc aatcacctga cgattcaagg tggttggatc
    14821 atgacgattc cagaaaacat caagcaagct ctcaaagcta cactctttgg gatcatactg
    14881 aactctaaca acctcgttat gtcccgtagt gccagtacag acatcctcgt aactcggatt
    14941 gtgcacgatg ccatggctat acccaacctc ggtcttggtc acaccaggaa ctctctggta
    15001 agctagctcc actccccaga aacaaccggc gccaaattgc gcgaattgct gacctgaaga
    15061 cggaacatca tcgtcgggtc cttgggcgat tgcggcggaa gatgggtcag cttgggcttg
    15121 aggacgagac ccgaatccga gtctgttgaa aaggttgttc attggggatt tgtatacgga
    15181 gattggtcgt cgagaggttt gagggaaagg acaaatgggt ttggctctgg agaaagagag
    15241 tgcggcttta gagagagaat tgagaggttt agagagagat gcggcggcga tgagcggagg
    15301 agagacgacg aggacctgca ttatcaaagc agtgacgtgg tgaaatttgg aacttttaag
    15361 aggcagatag atttattatt tgtatccatt ttcttcattg ttctagaatg tcgcggaaca
    15421 aattttaaaa ctaaatccta aatttttcta attttgttgc caatagtgga tatgtgggcc
    15481 gtatagaagg aatctattga aggcccaaac ccatactgac gagcccaaag gttcgttttg
    15541 cgttttatgt ttcggttcga tgccaacgcc acattctgag ctaggcaaaa aacaaacgtg
    15601 tctttgaata gactcctctc gttaacacat gcagcggctg catggtgacg ccattaacac
    15661 gtggcctaca attgcatgat gtctccattg acacgtgact tctcgtctcc tttcttaata
    15721 tatctaacaa acactcctac ctcttccaaa atatatacac atctttttga tcaatctctc
    15781 attcaaaatc tcattctctc tagtaaacaa gaacaaaaaa atggcggata cagctagagg
    15841 aacccatcac gatatcatcg gcagagacca gtacccgatg atgggccgag accgagacca
    15901 gtaccagatg tccggacgag gatctgacta ctccaagtct aggcagattg ctaaagctgc
    15961 aactgctgtc acagctggtg gttccctcct tgttctctcc agccttaccc ttgttggaac
    16021 tgtcatagct ttgactgttg caacacctct gctcgttatc ttcagcccaa tccttgtccc
    16081 ggctctcatc acagttgcac tcctcatcac cggttttctt tcctctggag ggtttggcat
    16141 tgccgctata accgttttct cttggattta caagtaagca cacatttatc atcttacttc
    16201 ataattttgt gcaatatgtg catgcatgtg ttgagccagt agctttggat caattttttt
    16261 ggtcgaataa caaatgtaac aataagaaat tgcaaattct agggaacatt tggttaacta
    16321 aatacgaaat ttgacctagc tagcttgaat gtgtctgtgt atatcatcta tataggtaaa
    16381 atgcttggta tgatacctat tgattgtgaa taggtacgca acgggagagc acccacaggg
    16441 atcagacaag ttggacagtg caaggatgaa gttgggaagc aaagctcagg atctgaaaga
    16501 cagagctcag tactacggac agcaacatac tggtggggaa catgaccgtg accgtactcg
    16561 tggtggccag cacactacta tgagcgagct gattaaggag aacatgcaca tgaagctgta
    16621 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
    16681 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
    16741 cttcgacatc ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca
    16801 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
    16861 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
    16921 cctcatctac aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca
    16981 gaagaaaaca ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct
    17041 ggaaggcaga agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt
    17101 caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta
    17161 tgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca
    17221 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca agtgatctag
    17281 taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc
    17341 tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa
    17401 cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat
    17461 aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga
    17521 acgataagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    17581 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    17641 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg agcttgagct
    17701 tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
    17761 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
    17821 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
    17881 atcaaagtac tttaaagtac tttaaagtac tttaa
//
