LOCUS       sequence-331094-       17650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 17650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU51
FEATURES             Location/Qualifiers
     source          1..17650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7710..11813
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7710..11813
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11826..11846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    12358..12482
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12507..12512,12513..12530,12531..12537)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             12591..13271
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(12657..12676)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             13591..13896
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     13775..13794
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(13937..14061)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             16315..17010
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      complement(17009..17260)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(17270..17287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(17270..17286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17279..17301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    17482..17506
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcaaaaatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
     6781 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
     6841 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
     6901 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
     6961 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
     7021 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
     7081 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
     7141 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
     7201 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
     7261 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
     7321 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
     7381 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
     7441 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
     7501 ctgttatatc atttttttta ttaattgtgt atatatatat gtgcatagat ctggattaca
     7561 tgattgtgat tatttacatg attttgttat ttacgtatgt atatatgtag atctggactt
     7621 tttggagttg ttgacttgat tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg
     7681 ttatgtatgt gcagcgaatt cggcgcgcca tggataagaa gtactctatc ggactcgata
     7741 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga
     7801 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc
     7861 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa
     7921 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg
     7981 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata
     8041 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga
     8101 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc
     8161 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg
     8221 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga
     8281 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta
     8341 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg
     8401 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta
     8461 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct
     8521 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc
     8581 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg
     8641 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg
     8701 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt
     8761 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag
     8821 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg
     8881 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc
     8941 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc
     9001 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact
     9061 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg
     9121 aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt
     9181 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
     9241 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
     9301 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
     9361 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
     9421 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
     9481 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
     9541 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
     9601 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
     9661 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
     9721 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
     9781 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
     9841 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
     9901 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
     9961 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
    10021 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
    10081 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
    10141 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
    10201 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
    10261 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
    10321 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
    10381 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
    10441 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
    10501 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
    10561 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
    10621 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
    10681 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
    10741 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
    10801 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
    10861 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
    10921 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
    10981 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
    11041 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
    11101 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
    11161 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
    11221 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
    11281 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
    11341 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
    11401 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
    11461 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
    11521 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
    11581 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
    11641 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
    11701 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
    11761 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaaggg
    11821 ctgatcctaa gaagaagagg aaggtttgag gcgcgccgag ctccaggcct cccagctttc
    11881 gtccgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt cattgcccac
    11941 acaccagaat cctactaagt ttgagtatta tggcattgga aaagctgttt tcttctatca
    12001 tttgttctgc ttgtaattta ctgtgttctt tcagtttttg ttttcggaca tcaaaatgca
    12061 aatggatgga taagagttaa taaatgatat ggtccttttg ttcattctca aattattatt
    12121 atctgttgtt tttactttaa tgggttgaat ttaagtaaga aaggaactaa cagtgtgata
    12181 ttaaggtgca atgttagaca tataaaacag tctttcacct ctctttggtt atgtcttgaa
    12241 ttggtttgtt tcttcactta tctgtgtaat caagtttact atgagtctat gatcaagtaa
    12301 ttatgcaatc aagttaagta cagtataggc ttgagctccc taggcctgtt atccctaaca
    12361 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
    12421 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
    12481 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
    12541 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
    12601 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
    12661 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
    12721 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
    12781 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
    12841 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
    12901 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
    12961 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
    13021 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
    13081 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
    13141 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
    13201 tgcttaatga attacaacag tactgcgatg agtggcaggc ggggcgtaat ctagaggatc
    13261 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag
    13321 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt
    13381 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata
    13441 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct
    13501 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct
    13561 cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga
    13621 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga
    13681 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc ccgtgaactt
    13741 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
    13801 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
    13861 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc
    13921 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg
    13981 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
    14041 agctttcttg tacaaagtgg ttcgataatt ccgatccagc ctaggcccgg gcctgaggac
    14101 gcgtccatgg ttaattaaga cgtccggacc gactagtgga tcctctagag tcgacctgca
    14161 ggcatgcaag cttcttcaag tgtatgtagg tatagtaaca tgaacaagaa cagactcaag
    14221 tacaagatcg catacgaaaa tggaaatggc aatgtcactt ccacataatc aaacacgaat
    14281 cctcatatca acaaggcctg agattctaac tagctcataa caacttagcc aatagttact
    14341 tgagactacc aaatgtatgt agaactaaag actaagggac agagagttcg tctaaacagg
    14401 tgaatctagt cgttgttatc taataaacaa ttcagcccca aatgcagaac acacatagag
    14461 ctctctattg attcaaatta cgatctgata ctgataacgt ctagattttt agggttaaag
    14521 caatcaatca cctgacgatt caaggtggtt ggatcatgac gattccagaa aacatcaagc
    14581 aagctctcaa agctacactc tttgggatca tactgaactc taacaacctc gttatgtccc
    14641 gtagtgccag tacagacatc ctcgtaactc ggattgtgca cgatgccatg gctataccca
    14701 acctcggtct tggtcacacc aggaactctc tggtaagcta gctccactcc ccagaaacaa
    14761 ccggcgccaa attgcgcgaa ttgctgacct gaagacggaa catcatcgtc gggtccttgg
    14821 gcgattgcgg cggaagatgg gtcagcttgg gcttgaggac gagacccgaa tccgagtctg
    14881 ttgaaaaggt tgttcattgg ggatttgtat acggagattg gtcgtcgaga ggtttgaggg
    14941 aaaggacaaa tgggtttggc tctggagaaa gagagtgcgg ctttagagag agaattgaga
    15001 ggtttagaga gagatgcggc ggcgatgagc ggaggagaga cgacgaggac ctgcattatc
    15061 aaagcagtga cgtggtgaaa tttggaactt ttaagaggca gatagattta ttatttgtat
    15121 ccattttctt cattgttcta gaatgtcgcg gaacaaattt taaaactaaa tcctaaattt
    15181 ttctaatttt gttgccaata gtggatatgt gggccgtata gaaggaatct attgaaggcc
    15241 caaacccata ctgacgagcc caaaggttcg ttttgcgttt tatgtttcgg ttcgatgcca
    15301 acgccacatt ctgagctagg caaaaaacaa acgtgtcttt gaatagactc ctctcgttaa
    15361 cacatgcagc ggctgcatgg tgacgccatt aacacgtggc ctacaattgc atgatgtctc
    15421 cattgacacg tgacttctcg tctcctttct taatatatct aacaaacact cctacctctt
    15481 ccaaaatata tacacatctt tttgatcaat ctctcattca aaatctcatt ctctctagta
    15541 aacaagaaca aaaaaatggc ggatacagct agaggaaccc atcacgatat catcggcaga
    15601 gaccagtacc cgatgatggg ccgagaccga gaccagtacc agatgtccgg acgaggatct
    15661 gactactcca agtctaggca gattgctaaa gctgcaactg ctgtcacagc tggtggttcc
    15721 ctccttgttc tctccagcct tacccttgtt ggaactgtca tagctttgac tgttgcaaca
    15781 cctctgctcg ttatcttcag cccaatcctt gtcccggctc tcatcacagt tgcactcctc
    15841 atcaccggtt ttctttcctc tggagggttt ggcattgccg ctataaccgt tttctcttgg
    15901 atttacaagt aagcacacat ttatcatctt acttcataat tttgtgcaat atgtgcatgc
    15961 atgtgttgag ccagtagctt tggatcaatt tttttggtcg aataacaaat gtaacaataa
    16021 gaaattgcaa attctaggga acatttggtt aactaaatac gaaatttgac ctagctagct
    16081 tgaatgtgtc tgtgtatatc atctatatag gtaaaatgct tggtatgata cctattgatt
    16141 gtgaataggt acgcaacggg agagcaccca cagggatcag acaagttgga cagtgcaagg
    16201 atgaagttgg gaagcaaagc tcaggatctg aaagacagag ctcagtacta cggacagcaa
    16261 catactggtg gggaacatga ccgtgaccgt actcgtggtg gccagcacac tactatgagc
    16321 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
    16381 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
    16441 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
    16501 atgtacggca gcagaacctt catcaaccac acccagggca tccccgactt ctttaagcag
    16561 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
    16621 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
    16681 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
    16741 aacaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg
    16801 aagctcgtgg gcgggggcca cctgatctgc aacttcaaga ccacatacag atccaagaaa
    16861 cccgctaaga acctcaagat gcccggcgtc tactatgtgg accacagact ggaaagaatc
    16921 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc
    16981 gacctcccta gcaaactggg gcacaagtga tctagtaaca tagatgacac cgcgcgcgat
    17041 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
    17101 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
    17161 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
    17221 ttcaatctta agaaacttta ttgccaaatg tttgaacgat aagcttggca ctggccgtcg
    17281 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    17341 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    17401 agttgcgcag cctgaatggc gaatgagctt gagcttggat cagattgtcg tttcccgcct
    17461 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
    17521 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
    17581 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa agtactttaa
    17641 agtactttaa
//
