LOCUS       sequence-331097-       16751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Final destination vector for single Gateway LR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Ursache R, Fujita S, Denervaud Tendon V, Geldner N
  TITLE     Combined fluorescent seed selection and multiplex CRISPR/Cas9 
            assembly for fast generation of multiple Arabidopsis mutants.
  JOURNAL   Plant Methods. 2021 Oct 30;17(1):111. doi: 
            10.1186/s13007-021-00811-9.
  PUBMED    34717688
REFERENCE   2  (bases 1 to 16751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2021-10-30"; volume: "17(1):111. doi"; pages: " 
            10.1186/s13007-021-00811-9"
COMMENT     Sequence Label: pRU320
FEATURES             Location/Qualifiers
     source          1..16751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2245..3318
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3384..3578
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3814..3836)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3922..4062
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3977..3996
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4077..4094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4248..4836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4328..4347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5082..5873)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6394..6418
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    6595..6616
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6631..6636,6637..6654,6655..6661)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6669..6685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6674..6696
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6693..6709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6693..6709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7732..10890
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNE
                     GRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEE
                     FSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGE
                     VRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFG
                     WKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKF
                     QIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKE
                     IIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKA
                     INLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSI
                     KVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENA
                     KYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVK
                     QEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRF
                     SVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKER
                     NKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEI
                     FITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKD
                     NDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKK
                     DNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKN
                     LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLL
                     NRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQ
                     IIKKG"
     CDS             10903..10923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    11435..11559
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(11584..11589,11590..11607,11608..11614)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             11668..12348
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(11734..11753)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             12668..12973
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     12852..12871
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(13014..13138)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(15392..15394,15395..15397,15398..16111)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(15392..15394,15395..15397,15398..16108)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(15437..15458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(15698..15717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     16045..16066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      complement(16110..16360)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(16371..16388)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16371..16387)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16380..16402)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    16583..16607
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
     2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
     2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
     2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
     2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
     2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
     2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
     2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
     2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
     2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
     2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
     2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
     2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
     3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
     3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
     3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
     3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
     3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
     3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
     3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
     3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
     3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
     3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
     3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
     3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
     3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
     3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
     4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
     4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aacgcttgag ttaagccgcg
     5041 ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac attatttgcc gactaccttg
     5101 gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag
     5161 cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg
     5221 gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt
     5281 actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag
     5341 tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca
     5401 ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt
     5461 gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca
     5521 agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac
     5581 ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct
     5641 ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca
     5701 agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc
     5761 actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg
     5821 ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttcccc catgatgttt
     5881 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     5941 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacccca
     6001 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     6061 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag cttacgaacc
     6121 gaacaggctt atgtccactg ggttcgtgcc cgaattgatc acaggcagca acgctctgtc
     6181 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     6241 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     6301 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     6361 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
     6421 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
     6481 cgaattgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6541 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6601 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6661 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     6721 ttcaaaaatt acggatatga atataggcat atccgtatcc gaattatccg tttgacagct
     6781 agcaacgatt gtacaattgc ttctttaaaa aaggaagaaa gaaagaaaga aaagaatcaa
     6841 catcagcgtt aacaaacggc cccgttacgg cccaaacggt catatagagt aacggcgtta
     6901 agcgttgaaa gactcctatc gaaatacgta accgcaaacg tgtcatagtc agatcccctc
     6961 ttccttcacc gcctcaaaca caaaaataat cttctacagc ctatatatac aaccccccct
     7021 tctatctctc ctttctcaca attcatcatc tttctttctc tacccccaat tttaagaaat
     7081 cctctcttct cctcttcatt ttcaaggtaa atctctctct ctctctctct ctctgttatt
     7141 ccttgtttta attaggtatg tattattgct agtttgttaa tctgcttatc ttatgtatgc
     7201 cttatgtgaa tatctttatc ttgttcatct catccgttta gaagctataa atttgttgat
     7261 ttgactgtgt atctacacgt ggttatgttt atatctaatc agatatgaat ttcttcatat
     7321 tgttgcgttt gtgtgtacca atccgaaatc gttgattttt ttcatttaat cgtgtagcta
     7381 attgtacgta tacatatgga tctacgtatc aattgttcat ctgtttgtgt ttgtatgtat
     7441 acagatctga aaacatcact tctctcatct gattgtgttg ttacatacat agatatagat
     7501 ctgttatatc atttttttta ttaattgtgt atatatatat gtgcatagat ctggattaca
     7561 tgattgtgat tatttacatg attttgttat ttacgtatgt atatatgtag atctggactt
     7621 tttggagttg ttgacttgat tgtatttgtg tgtgtatatg tgtgttctga tcttgatatg
     7681 ttatgtatgt gcagcgaatt cggcgcgcct gtgcagcgaa ttcggcgcgc catgaagagg
     7741 aactacatcc tcggactcga tatcggaatc acctctgtgg gatacggaat catcgattac
     7801 gagactaggg atgtgatcga tgctggtgtg agactcttca aagaggctaa cgttgagaac
     7861 aacgagggaa gaaggtctaa gaggggagct agaaggctca agagaagaag aaggcacaga
     7921 atccagaggg tgaagaagct cctcttcgat tacaacctcc tcaccgatca ctctgagctt
     7981 tctggaatca acccttacga ggcaagagtg aagggactct ctcagaagtt gtctgaggaa
     8041 gagttctctg ctgctttgct ccaccttgct aagagaaggg gtgtgcataa cgtgaacgag
     8101 gtggaagagg ataccggaaa cgagctttct accaaagagc agatctctag gaactctaag
     8161 gctctcgaag agaagtacgt ggcagagctt cagcttgaga gactcaagaa agatggtgag
     8221 gtgaggggat ctatcaacag gttcaagacc tctgattacg tgaaagaagc taagcagctc
     8281 ctcaaggtgc agaaggctta ccatcagctc gatcagtctt tcatcgatac ctacatcgat
     8341 cttctcgaga ctagaaggac ctactacgag ggacctggtg aaggatctcc attcggatgg
     8401 aaggatatca aagaatggta cgagatgctc atgggacact gcacttactt ccctgaagaa
     8461 ctcagatctg tgaagtacgc ttacaacgct gatctctaca acgctcttaa cgatctcaac
     8521 aacctcgtga tcaccaggga tgagaacgag aagcttgagt actacgagaa gttccagatc
     8581 atcgagaacg tgttcaagca aaagaagaag cctaccctca agcagatcgc taaagagatc
     8641 cttgttaacg aggaagatat caagggatac agggtgacct ctaccggaaa gcctgagttc
     8701 actaacctca aggtttacca cgatattaag gatattaccg ctagaaaaga gattattgag
     8761 aacgctgagc ttctcgatca aatcgctaag atccttacca tctaccagtc atctgaggat
     8821 atccaagagg aattgaccaa cctcaactca gagcttaccc aagaagagat cgagcagatc
     8881 agtaacttga agggttacac cggaacccac aacctctcac tcaaggctat caacctcatc
     8941 ctcgatgagc tttggcacac caacgataac cagatcgcaa tcttcaacag actcaagctc
     9001 gtgcctaaga aagtggatct ctctcagcag aaagagattc ctaccaccct cgtggatgat
     9061 ttcatcctct cacctgttgt gaagagatct ttcatccagt ctatcaaggt gatcaacgct
     9121 attatcaaga aatacggact ccctaacgat atcatcatcg agttggctag ggaaaagaac
     9181 tcaaaggatg ctcaaaagat gatcaacgag atgcagaaga gaaacaggca gaccaacgag
     9241 aggatcgaag agatcatcag gaccaccgga aaagagaacg ctaagtacct cattgagaag
     9301 atcaagctcc acgatatgca agagggaaag tgcctctact ctctcgaggc tatccctctt
     9361 gaggatctcc ttaacaaccc tttcaactac gaagtggatc acatcatccc tagatctgtg
     9421 tctttcgata actctttcaa caacaaggtg ctcgtgaagc aagaggaaaa ctctaagaag
     9481 ggtaacagga cccctttcca gtacctctct tcatctgatt ctaagatctc atacgagact
     9541 ttcaagaagc acatcctcaa cctcgctaag ggaaagggta ggatctctaa gacaaagaaa
     9601 gagtaccttc tcgaggaaag ggatatcaac agattctctg ttcagaagga tttcatcaac
     9661 agaaaccttg tggataccag gtacgctacc aggggtctta tgaaccttct caggtcttac
     9721 ttcagggtga acaaccttga tgtgaaggtg aagtcaatca acggtggatt cacctcattc
     9781 cttagaagaa aatggaagtt taagaaagag aggaacaagg gttataagca ccacgctgag
     9841 gatgctctca ttatcgctaa cgcagatttc attttcaaag agtggaagaa gttggataag
     9901 gctaaaaagg tgatggaaaa ccagatgttc gaggaaaagc aggctgagtc tatgcctgag
     9961 atcgagactg agcaagagta caaagaaatc ttcatcaccc ctcaccagat caagcacatc
    10021 aaagatttca aggattacaa gtactctcac agagtggata agaagccaaa cagagagctt
    10081 atcaacgata ccctctactc aaccagaaag gatgataagg gaaacaccct catcgtgaac
    10141 aatctcaacg gactctacga taaggataac gataagctca agaagctcat taacaagtct
    10201 ccagagaagt tgctcatgta ccaccacgat cctcagacct accaaaagct caagctcatc
    10261 atggaacagt acggtgatga gaagaaccct ctctacaagt attacgagga aaccggtaac
    10321 tacctcacca agtactcaaa aaaggataat ggacctgtga tcaagaagat taagtactac
    10381 ggaaacaagc tcaacgcaca cctcgatatc accgatgatt accctaacag tagaaacaag
    10441 gtggtgaagc tttcactcaa gccttacagg ttcgatgtgt acctcgataa cggtgtgtac
    10501 aagttcgtga ccgtgaagaa cctcgatgtt atcaaaaaag aaaactacta tgaggttaac
    10561 tctaagtgct acgaggaagc taagaagttg aagaagatct ctaaccaggc agagttcatt
    10621 gcttcattct acaacaacga tcttatcaag attaacggtg agctttacag ggttatcgga
    10681 gtgaacaacg atttgctcaa caggatcgag gtgaacatga ttgatatcac ctacagggaa
    10741 tacctcgaga atatgaacga taagaggcct cctagaatca tcaagactat cgcttctaag
    10801 acccagagta tcaagaagta ctctaccgat atcctcggaa acctctacga ggttaagtca
    10861 aagaagcacc ctcagatcat taagaaggga tcaagggctg atcctaagaa gaagaggaag
    10921 gtttgaggcg cgccgagctc caggcctccc agctttcgtc cgtatcatcg gtttcgacaa
    10981 cgttcgtcaa gttcaatgca tcagtttcat tgcccacaca ccagaatcct actaagtttg
    11041 agtattatgg cattggaaaa gctgttttct tctatcattt gttctgcttg taatttactg
    11101 tgttctttca gtttttgttt tcggacatca aaatgcaaat ggatggataa gagttaataa
    11161 atgatatggt ccttttgttc attctcaaat tattattatc tgttgttttt actttaatgg
    11221 gttgaattta agtaagaaag gaactaacag tgtgatatta aggtgcaatg ttagacatat
    11281 aaaacagtct ttcacctctc tttggttatg tcttgaattg gtttgtttct tcacttatct
    11341 gtgtaatcaa gtttactatg agtctatgat caagtaatta tgcaatcaag ttaagtacag
    11401 tataggcttg agctccctag gcctgttatc cctaacaagt ttgtacaaaa aagctgaacg
    11461 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
    11521 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
    11581 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
    11641 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
    11701 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
    11761 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
    11821 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
    11881 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
    11941 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
    12001 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
    12061 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
    12121 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
    12181 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
    12241 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
    12301 tgcgatgagt ggcaggcggg gcgtaatcta gaggatccgg cttactaaaa gccagataac
    12361 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
    12421 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
    12481 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
    12541 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
    12601 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
    12661 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
    12721 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
    12781 tgcacgtctg ctgtcagata aagtcccccg tgaactttac ccggtggtgc atatcgggga
    12841 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
    12901 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
    12961 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
    13021 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
    13081 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttc
    13141 gataattccg atccagccta ggcccgggcc tgaggacgcg tccatggtta attaagacgt
    13201 ccggaccgac tagtggatcc tctagagtcg acctgcaggc atgcaagctt cttcaagtgt
    13261 atgtaggtat agtaacatga acaagaacag actcaagtac aagatcgcat acgaaaatgg
    13321 aaatggcaat gtcacttcca cataatcaaa cacgaatcct catatcaaca aggcctgaga
    13381 ttctaactag ctcataacaa cttagccaat agttacttga gactaccaaa tgtatgtaga
    13441 actaaagact aagggacaga gagttcgtct aaacaggtga atctagtcgt tgttatctaa
    13501 taaacaattc agccccaaat gcagaacaca catagagctc tctattgatt caaattacga
    13561 tctgatactg ataacgtcta gatttttagg gttaaagcaa tcaatcacct gacgattcaa
    13621 ggtggttgga tcatgacgat tccagaaaac atcaagcaag ctctcaaagc tacactcttt
    13681 gggatcatac tgaactctaa caacctcgtt atgtcccgta gtgccagtac agacatcctc
    13741 gtaactcgga ttgtgcacga tgccatggct atacccaacc tcggtcttgg tcacaccagg
    13801 aactctctgg taagctagct ccactcccca gaaacaaccg gcgccaaatt gcgcgaattg
    13861 ctgacctgaa gacggaacat catcgtcggg tccttgggcg attgcggcgg aagatgggtc
    13921 agcttgggct tgaggacgag acccgaatcc gagtctgttg aaaaggttgt tcattgggga
    13981 tttgtatacg gagattggtc gtcgagaggt ttgagggaaa ggacaaatgg gtttggctct
    14041 ggagaaagag agtgcggctt tagagagaga attgagaggt ttagagagag atgcggcggc
    14101 gatgagcgga ggagagacga cgaggacctg cattatcaaa gcagtgacgt ggtgaaattt
    14161 ggaactttta agaggcagat agatttatta tttgtatcca ttttcttcat tgttctagaa
    14221 tgtcgcggaa caaattttaa aactaaatcc taaatttttc taattttgtt gccaatagtg
    14281 gatatgtggg ccgtatagaa ggaatctatt gaaggcccaa acccatactg acgagcccaa
    14341 aggttcgttt tgcgttttat gtttcggttc gatgccaacg ccacattctg agctaggcaa
    14401 aaaacaaacg tgtctttgaa tagactcctc tcgttaacac atgcagcggc tgcatggtga
    14461 cgccattaac acgtggccta caattgcatg atgtctccat tgacacgtga cttctcgtct
    14521 cctttcttaa tatatctaac aaacactcct acctcttcca aaatatatac acatcttttt
    14581 gatcaatctc tcattcaaaa tctcattctc tctagtaaac aagaacaaaa aaatggcgga
    14641 tacagctaga ggaacccatc acgatatcat cggcagagac cagtacccga tgatgggccg
    14701 agaccgagac cagtaccaga tgtccggacg aggatctgac tactccaagt ctaggcagat
    14761 tgctaaagct gcaactgctg tcacagctgg tggttccctc cttgttctct ccagccttac
    14821 ccttgttgga actgtcatag ctttgactgt tgcaacacct ctgctcgtta tcttcagccc
    14881 aatccttgtc ccggctctca tcacagttgc actcctcatc accggttttc tttcctctgg
    14941 agggtttggc attgccgcta taaccgtttt ctcttggatt tacaagtaag cacacattta
    15001 tcatcttact tcataatttt gtgcaatatg tgcatgcatg tgttgagcca gtagctttgg
    15061 atcaattttt ttggtcgaat aacaaatgta acaataagaa attgcaaatt ctagggaaca
    15121 tttggttaac taaatacgaa atttgaccta gctagcttga atgtgtctgt gtatatcatc
    15181 tatataggta aaatgcttgg tatgatacct attgattgtg aataggtacg caacgggaga
    15241 gcacccacag ggatcagaca agttggacag tgcaaggatg aagttgggaa gcaaagctca
    15301 ggatctgaaa gacagagctc agtactacgg acagcaacat actggtgggg aacatgaccg
    15361 tgaccgtact cgtggtggcc agcacactac tatggtgagc aagggcgagg agctgttcac
    15421 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
    15481 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
    15541 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
    15601 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
    15661 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
    15721 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
    15781 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
    15841 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
    15901 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
    15961 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
    16021 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
    16081 cactctcggc atggacgagc tgtacaagtg atctagtaac atagatgaac cgcgcgcgat
    16141 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
    16201 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
    16261 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
    16321 ttcaatctta agaaacttta ttgccaaatg tttgaacgat gaagcttggc actggccgtc
    16381 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    16441 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    16501 cagttgcgca gcctgaatgg cgaatgagct tgagcttgga tcagattgtc gtttcccgcc
    16561 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
    16621 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
    16681 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtacttta aagtacttta
    16741 aagtacttta a
//
